1.Effect of Male Sex Hormones on Calcium Oxalate Nephrolithiasis in Ethylene Glycol-Treated Rats.
Young Tae MOON ; Seung Hwan YOON ; Mi Kyung KIM
Korean Journal of Urology 2001;42(3):273-278
PURPOSE: Sexual differences in the incidence and crystalline composition of urinary stones in humans are well-known, but it is unclear why men have a higher incidence of calcium oxalate stones than women. We investigated the effects of male sex hormones on stone formation using an ethylene glycol (EG) - induced urolithiasis model in rats. MATERIALS AND METHODS: Adult male Sprague-Dawley rats were divided into 4 groups, each containing 10 rats. One group of rats was left untreated and served as control. The other 3 groups were fed a 1% ethylene glycol (EG) lithogenic diet for 4 weeks. Among these, one group was non-castrated, one group was castrated and one group was non-castrated and given finasteride orally. Serum testosterone, creatinine, electrolytes, 24-hour urine levels of oxalate and citrate, and creatinine clearance were measured. The crystal deposits were examined by light and polarizing microscopes. RESULTS: Testosterone promoted calcium oxalate stone formation in EG - treated rats. Finasteride administration significantly decreased urinary oxalate excretion and calcium oxalate deposition, compared with controls. Urinary citrate was significantly decreased in EG-treated rats, but was not influenced by castration or administration of finasteride. There were no significant differences in serum concentrations of creatinine, sodium, or potassium among the control and experimental groups. CONCLUSIONS: Our data suggest that testosterone promotes calcium oxalate stone formation, and that dihydrotestosterone may be partially responsible for the exaggerated hyperoxaluria in EG-treated rats. Additionally, male sex hormones have a lesser influence on urinary citrate than oxalate.
Adult
;
Animals
;
Calcium Oxalate*
;
Calcium*
;
Castration
;
Citric Acid
;
Creatinine
;
Crystallins
;
Diet
;
Dihydrotestosterone
;
Electrolytes
;
Ethylene Glycol
;
Female
;
Finasteride
;
Gonadal Steroid Hormones*
;
Humans
;
Hyperoxaluria
;
Incidence
;
Male*
;
Nephrolithiasis*
;
Potassium
;
Rats*
;
Rats, Sprague-Dawley
;
Sodium
;
Testosterone
;
Urinary Calculi
;
Urolithiasis
2.A clinical study of seasonal affective disorder.
Kyung Kyu LEE ; Do Joon YOON ; Hwan Il CHANG
Journal of Korean Neuropsychiatric Association 1992;31(1):84-92
No abstract available.
Seasonal Affective Disorder*
;
Seasons*
3.Hereditary Nonpolyposis Colorectal Cancer.
Hwan Nam GUNG ; Seok Hwan LEE ; Sung Hwan HONG ; Choong YOON ; Kee Hyung LEE
Journal of the Korean Society of Coloproctology 1997;13(3):363-374
Hereditary nonpolyposis colorectal cancer(HNPCC) is an autosomal dominantly inherited disease associated with a marked increase in cancer susceptibility, especially cancer of the colorectum. The frequency of HNPCC in the general population is yet to be determined, but HNPCC may account for as much as 2% to 5% of colorectal cancer, Colorectal cancer in HNPCC differs from sporadic colorectal cancer by an early age of cancer onset, proximal predominance of colorectal cancer, an excess of synchronous and metachronous colorectal cancer, and excess extra-colonic cancers. We have found 5 HNPCC families since 1992 when we reported first HNPCC family (KHU-Hl) In order to register the patients of HNPCC and to review the clinicopathologic feature and appropriate management, we have analysed 5 HNPCC families. Five HNPCC families included 16 colorectal cancer patients(14 males and 2 females). The average age of first diagnosis was 39. Among 16 patients, 8 patient were operated at the KyungHee University hospital and their operative and pathologic records were available. Two synchronous and seven metachronous cancers were founded, so that eight patients had 15 colorectal cancer lesions. Ten cancers were located proximal to splenic flexure and five were distal. Partial resection of colon was performed in seven cases except one when the first diagnosis was made and recurrence was founded in 5 patients. Recurrence was treated by total colectomy in 3 cases and subtotal colectomy in two. In conclusion, we re-confirmed that HNPCC patient should be treated by no less than a subtotal colectomy because of high multiplicity and high recurrence rate of partial resection.
Colectomy
;
Colon
;
Colon, Transverse
;
Colorectal Neoplasms
;
Colorectal Neoplasms, Hereditary Nonpolyposis*
;
Diagnosis
;
Humans
;
Male
;
Recurrence
4.Identification of Oocyte-Specific Diva-Associated Proteins using Mass Spectrometry.
Se Jin YOON ; Jung Woong KIM ; Kyung Hee CHOI ; Sook Hwan LEE ; Kyung Ah LEE
Korean Journal of Fertility and Sterility 2006;33(3):189-198
OBJECTIVE: We previously described that Diva is highly expressed in matured metaphase II (MII) oocytes compared to immature germinal vesicle (GV) oocytes in mouse.1 We report here that the expression of Diva transcript as well as protein is oocyte-specific. To elucidate its physiological role in oocyte, the binding partner(s) of Diva has been identified by using immunoprecipitation (IP) followed by Mass Spectrometry. METHODS: NIH/3T3 cells were transiently transfected for 24 h with either empty vector for control or FLAG-tagged mouse Diva construct, and IP was performed with anti-FLAG antibody. The immuno-isolated complexes were resolved by SDS-PAGE on a 12% gel followed by Coomassie Blue staining. For in-gel digestion, 15 bands of interest were excised manually and digested with trypsin. All mass spectra were acquired at a positive reflector mode by a 4700 Proteomics Analyzer (Applied Biosystems, Framingham, MA). Proteins were identified by searching the NCBI nonredundant database using MASCOT Peptide Mass Fingerprint software (Matrixscience, London). RESULTS: Diva-associated complexes were formed in FLAG-tagged mouse Diva-overexpressed NIH/3T3 cells via IP using anti-FLAG-conjugated beads. Among the excised 15 bands, actin and actin-binding proteins such as tropomyosin, tropomodulin 3, and alpha-actinin were identified. Binding between Diva and actin or tropomyosin was confirmed by IP followed by Western blot analysis. Both bindings were also detected endogenously in mouse ovaries, indicating that Diva works with actin and tropomyosin. CONCLUSIONS: This is the first report that immuno-isolated Diva-associated complexes are related to actin filament of the cytoskeletal system. When we consider the association of Diva with actin and tropomyosin, oocyte-specific Diva may play a role in modulating the cytoskeletal system during oocyte maturation.
Actin Cytoskeleton
;
Actinin
;
Actins
;
Animals
;
Blotting, Western
;
Dermatoglyphics
;
Digestion
;
Electrophoresis, Polyacrylamide Gel
;
Female
;
Immunoprecipitation
;
Mass Spectrometry*
;
Metaphase
;
Mice
;
Microfilament Proteins
;
Oocytes
;
Ovary
;
Proteomics
;
Tropomodulin
;
Tropomyosin
;
Trypsin
5.Computed Tomography(CT) in Head Trauma Patients with Alert Mental Status : How Important are the Clinical Symptoms.
Kyung Hwan KIM ; Kyung Ho LEE ; Won Yul KIM ; Young Chul YOON ; Hong Yong KIM
Journal of the Korean Society of Emergency Medicine 1997;8(4):564-570
STUDY OBJECTIVES: To identify the value of symptoms in head trauma patients with alert mental status requiring a head CT scan. METHODS: A retrospective study was performed at an emergency center over 8 months period. Patients(age > or = 7) were enrolled if they presented with a Glasgow Coma Scale score of 15 and underwent head CT after head trauma. The symptoms and signs were recorded in the entry forms prior to head CT The symptoms found in the patients with normal CT were compared with those in abnormal CT showing intracranial injuries. RESULTS: Among the 168 patients, traumatic intracranial abnormality was identified in 34(20.2%) by CT. Skull fracture was idendified in 8(4.8%) in normal CT, 17(10.1%) in abnormal CT group by simple X-ray and CT. In the normal CT group without skull fracture, 65(52.0%) had headache, 61(48.8%) had loss of consciousness( LOC), 43(34.4%) had vomiting, 24(19.2%) had nausea, 7(5.6%) had dizziness. In the abnormal CT group without skull fracture, 14(77.8%) had heacache, 9(50.0%) had LOC, 9(50.0%) had vomiting, 2(11.1%) had nausea, 2(11.1%) had dizziness. The patients with headache were significantly more common in the abnormal CT group. CONCLUSION: A head CT is required in alert head trauma patients with any symptoms and signs of intracranial injuries, especially with headache.
Craniocerebral Trauma*
;
Dizziness
;
Emergencies
;
Glasgow Coma Scale
;
Head*
;
Headache
;
Humans
;
Nausea
;
Retrospective Studies
;
Skull Fractures
;
Tomography, X-Ray Computed
;
Vomiting
6.The Effect of Small Doses of Oral Corticosteroids in Vitiligo Patients.
Tae Kee MOON ; Sung Bin IM ; Seung Kyung HANN ; Sung Hwan CHO ; Yoon Kee PARK
Korean Journal of Dermatology 1995;33(5):880-885
BACKGROUND: One of the most, probable pathogenesis of vitiliga is autoimmune. Systemic cor tico st,eroids suppress immunity and may arrest the progression of vitiligo and lead to repigmentation. OBJECTIVE: We have assessed the clinical effect of a oral small oral dose of corticosteroid to minimize side effects in vitiligo patients. METHODS: Thirty four patients(9;male, 25;female) with vitiligo were evaluated in this study. The patients took 7.5mg-20mg prednisolone initially for 2 months and then the dosage was tapered to half of the initial dosiat the 3rd month and half of dose of 3rd month for the last 4th month. We compared the effcct of treatment of vitiligo before and aft.er the study by photographs. and side efferts were issessed at. 1, 2, 3 and 4 month. RESULTS: The arrest of the progression of vitiligo was noticed in 79% of patients and repigmentation was noticed in 59% of patients which is statistically significant. The effect, of treatment according to extent, duiation, type, and site of vitiligo were not statistically significant. The side effects of treatment were minimal and did not affect the course of treatment. CONCLUSION: Small doses of iral corticosteroids are effective without any significant side effects in preventing progression and loiiduce repigmentation of active spreading vitiligo and generalized type of vitiligo that is difficult to treat with topical corticosteroids.
Adrenal Cortex Hormones*
;
Humans
;
Prednisolone
;
Vitiligo*
7.Epidermal Grafting after Removal of Recipient Epidermis by CO2 Laser Ablation in Vitiligo.
Han Kyoung CHO ; Seung Kyung HANN ; Jung Bin KIM ; Sung Hwan CHO ; Yoon Kee PARK
Korean Journal of Dermatology 1995;33(5):867-872
BACKGROUND: Epidermal grafting using cryotherapy for recipient sites is in widespread use. However the peripheral hypopigmented haloes that occur around the recipient sites require prolon gation of the treatment period. OBJECTIVE: We used a CO2 laser to remove the epidermis of the recipient sites for betteri results. METHODS: We treated lie localized vitiligo patients with CO2 laser to remove t.he epidermis and grafted suction blister rooves. We observed repigmentation and complications 1 month later. RESULTS & CONCLUSIONS : The superiority of this method is demonstrated by the fact 1) all prodedures can be completed on the day of operation 2) the incidence of hypertrophic scar and peripheral hypopigmented halos can be observed.The problems of this method are 1) uneven repigmentation of recipient. sites 2) hyperpigrnentation of recipient sites
Blister
;
Cicatrix, Hypertrophic
;
Cryotherapy
;
Epidermis*
;
Humans
;
Incidence
;
Lasers, Gas*
;
Suction
;
Transplants*
;
Vitiligo*
8.Reference(cut-off) values of serum total cholesterol for risk groupof atherosclerosis among normal adults in Korea.
Jong Seok PARK ; Dong Yoon KO ; Kyung Hwan CHO ; Myung Ho HONG ; Soon Duck KIM
Journal of the Korean Academy of Family Medicine 1992;13(2):152-163
No abstract available.
Adult*
;
Atherosclerosis*
;
Cholesterol*
;
Humans
;
Korea*
9.Repair of Microform Cleft Lip with Minimal Incision.
Byung Doo MIN ; Seung Ha PARK ; Eul Sik YOON ; Sang Hwan KOO ; Woo Kyung KIM
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1999;26(5):834-837
Microform cleft lip is a mild form of incomplete cleft lip, also known as a minimal occult, abortive, forme fruste cleft lip. However, it has no definition and few methods have been reported for its correction. A microform cleft lip is characterized as the incomplete union of the superficial portion of the orbicularis oris muscle. It is more prominent during facial expression than in a resting state. We confined microform cleft lip in our study to the absence of philtral skin change and a contracted position on the top of cupid's bow. During the past 5 years, 17 patients of microform cleft lip were operated on. We corrected the defect of the upper vermilion border and nostril sill with minimal incision, and repaired the underlying lip musculature in superficial discontinuity. Reduction of the widened alar base was performed. Deformed alar cartilage was dissected via rim incision, and suspended in a medial and upper direction with pull-out sutures. The most important thing is precise repair of the superficial portion of the separated orbicularis oris muscle via minimal incision, and it is best to operate after 1-year of age for accurate repair. The results were satisfactory and the parents were also satisfied. The advantages of this procedure are as follows: 1. Less visible, minimal scar on upper lip 2. Simultaneous correction of vermillion notching, deformed cupid's bow and nasal deformity. 3. Eversion of philtral ridge due to tenting effect of horizontal mattress suture 4. Philtral elongation effect by reduction of alar base and Z-plasty of cupid's bow.
Cartilage
;
Cicatrix
;
Cleft Lip*
;
Congenital Abnormalities
;
Facial Expression
;
Humans
;
Lip
;
Microfilming*
;
Parents
;
Skin
;
Sutures
10.Criminal liability of medical professionals.
Journal of the Korean Medical Association 2013;56(8):655-664
The Medical Service Act and many other laws regulate the actions of medical professionals. Receiving rebates from pharmaceutical companies has been criticized as unethical but not punished until 2011. However, it is now strongly forbidden. Unlicensed acts of medical care, which include providing medical care beyond the scope of the license or giving directions to an unlicensed person to practice medical care, are strictly punished even in the case of licensed medical professionals. It recently became an issue whether a doctor who wrote prescriptions to patients after examining them over the telephone violated the Medical Service Act. In addition, it is necessary to pay special attention to the administration of propofol since it recently became a major controversy. Furthermore, public health doctors are legally forbidden to work outside of a public health institution. The number of regulations on the medical industry is increasing every day. New laws usually toughen up punishment for those violating regulations. There is a legal maxim that says, "Ignorance of law excuses no one." Therefore, it is necessary for medical professionals to steadily study and become familiar with applicable laws and related criminal cases to prevent themselves from becoming criminally liable.
Criminals
;
Humans
;
Jurisprudence
;
Licensure
;
Prescriptions
;
Propofol
;
Public Health
;
Punishment
;
Social Control, Formal
;
Telephone