No abstract available.
Blood Platelets* ; Fetal Growth Retardation* ; Platelet Count* ; Pregnancy*

Spirochetes were isolated from the midgut of Ixodes persulcatus ticks captured at Chungju, Korea and identified as Borrelia afzelii strains by polymerase chain reaction. To determine the pathogenicity of the B. afzelii strains isolated in Korea, the microbiological and pathological features of Lyme disease were observed in C3H/He mice after intraperitoneal inoculation of the fresh isolate of B. afzelii strain. The results are summarized as follows 1) The Borrelia were detected in the tissues of heart, spleen, kidney, urinary bladder and knee joint within 7 days after inoculation of infection by dark field microscopic examination. The isolation rate from heart, urinary bladder and joint was significantly higher than the rate from spleen, kidney, and blood samples. 2) The Borrelia was detected in heart muscle by indirect immunofluorescent antibody test. 3) Antibody to the Borrelia was detected as early as one week after inoculation. 4) The marked tropism of the Borrelia was observed in myocardial, urinary tract and joint tissue. The main pathological features are inflammation in tissues of heart, kidney, joint and urinary bladder. From these results, the Borrelia afzelii strain isolated in Korea were determined as pathogenic strain.
Animals ; Borrelia burgdorferi Group* ; Borrelia burgdorferi* ; Borrelia* ; Chungcheongbuk-do ; Heart ; Inflammation ; Ixodes ; Joints ; Kidney ; Knee Joint ; Korea* ; Lyme Disease ; Mice ; Myocardium ; Pathology ; Polymerase Chain Reaction ; Spirochaetales ; Spleen ; Ticks ; Tropism ; Urinary Bladder ; Urinary Tract ; Virulence*

No abstract available.
Ameloblastoma* ; Biopsy, Fine-Needle*

To study the biology of the endothelium and the media of the vascular wall, full layer vascular wall model was constructed in vitro. In the experimental vascular wall model, endothelial cell (EC)s were grown on a collagen lattice containing multilayers of smooth muscle cell (SMC)s and a EC-free portion was made by a cloning ring on the culture disc. As conditioned culture media of ECs-SMCs contain biologic mediators that may promote the growth of SMCs, the availability of this vascular wall model promptly us to examine the extent to which ECs regulate the migration and proliferation of SMCs when these cells are maintained with or without covering EC lining in coculture. Morphologic characteristics of full layer vascular wall model was a whitish, non-transparent membrane. Outer boundaries and the zone of no EC were thicker than that of central portion. By light microscope imaging, luminal surface was composed of EC monolayer, and SMCs and collagen fibers were distributed between the polyethylene terephtalate (PET) membrane and EC monolayer. SMCs and collagen fibers were mainly located near the PET membrane. Venous SMCs were densely infiltrated as compared to arterial SMCs. By scanning electron microscopy, EC monolayer and dense collagen fibers in the zone of no EC were clearly shown. On the effects of platelet derived growth factor (PDGF) in the proliferation of SMCs and modeling of full layer vascular wall model, no effect on SMC in the zone of EC covering was seen however, active migration and proliferation of SMCs were noted in the zone of no EC. Wall thickness was two times greater than that of control. On the effects of EGF, it was observed that EGF markedly stimulated migration of SMCs with or without EC coverings in contrast to the control group. On the effects of FGF, results were similar to the PDGF group. Results on the effect of IGF-1 were similar to the PDGF group. As conclusions, full layer vascular wall model in this study was proved to be a good laboratory model for basic vascular research. And SMCs migration and proliferation were more active in venous SMCs compared to arterial SMCs. The collagen fibers were also richer and the wall was more thickened. EGF was most the potent SMC stimulator. PDGF, FGF, and IGF-1 were moderate SMC stimulator in the zone of no EC covering. These results strongly support why intimal hyperplasia eventually occured in autogenous venous bypass graft.
Biology ; Clone Cells ; Cloning, Organism ; Coculture Techniques ; Collagen ; Culture Media, Conditioned ; Endothelial Cells ; Endothelium ; Epidermal Growth Factor ; Hyperplasia ; Insulin-Like Growth Factor I ; Membranes ; Microscopy, Electron, Scanning ; Muscle, Smooth ; Myocytes, Smooth Muscle ; Phenobarbital ; Platelet-Derived Growth Factor ; Polyethylene ; Transplants

PURPOSE: A prospective comparison was made between imaging with Tc-99m pertechnetate (Tc-99m) and Ioine-131 (I-131) for the detection of residual and metastatic tissue after total thyroidectomy in patients with well-differentiated thyroid carcinoma. MATERALS AND METHODS: Initially our patients had imaging with Tc-99m, followed by I-131 within 3 days. The study included 21 patients who had ablation with high dose of I-131 ranging from 100 mCi to 150 mCi. Planar and pinhole images were acquired for both Tc-99m and I-131. Diagnostic images of Tc-99m and I-131 were compared with post-therapy images. Degree of uptake on Tc-99m and I-131 images was scored by four point scale and compared. RESULTS: The results of the Tc-99m study were: 16 of 19 studies (84%) were positive on simple planar images, but 19 of 20 studies (95%) were positive on pinhole images. Conventional I-131 diagnostic imaging on the other hand showed that all studies (100%) were positive on both planar and pinhole images. There was a significant difference in degree of uptake between Tc-99m and I-131 planar images (p<0.05). Only one case of Tc-99m scintigraphy was negative on both planar and pinhole studies (false negative). There was no distant metastasis on the therapeutic I-131 images. CONCLUSION: Tc-99m scan using pinhole in certain clinical situations is an alternative to the I-131 scan in detecting thyroid or lymph node metastasis prior to the first ablative treatment after thyroidectomy for well-differentiated thyroid carcinoma.
Diagnostic Imaging ; Hand ; Humans ; Lymph Nodes ; Neoplasm Metastasis ; Prospective Studies ; Radionuclide Imaging* ; Sodium Pertechnetate Tc 99m* ; Thyroid Gland* ; Thyroid Neoplasms* ; Thyroidectomy

No abstract available.
Female* ; Humans ; Transplants*

No abstract available.
Teratoma* ; Turner Syndrome*

Recently, we experienced the conservative management of ovarian cyst torsion using transvaginal ultrasonography-guided aspiration in 39 years old woman at 9 weeks of gestational age. The patient had a 7.3 cm-sized ovarian cyst suspected torsion. The symptom was acute lower abdominal pain. Transvaginal cyst aspiration was performed twice. The symptom was resolved after the procedures. The full term delivery was done by vaginal route and the ovarian cyst disappeared.
Abdominal Pain ; Adult ; Female ; Gestational Age ; Humans ; Ovarian Cysts* ; Pregnancy*

PURPOSE: To observe the histopathological changes in the periodontal tissues of mandibular molars in streptozotocin-induced diabetic rats after irradiation. MATERIALS AND METHODS: The male Sprague-Dawley rats weighing approximately 250 gm were divided into four groups: control, diabetes, irradiation, and diabetes- irradiation groups. Diabetes mellitus was induced in the rats by injecting streptozotocin. Rats in the control and irradiation groups were injected with citrate buffer only. After 5 days, the head and neck region of the rats in irradiation and diabetes-irradiation groups were irradiated with a single absorbed dose of 10 Gy. All the rats were sacrificed at 3, 7, 14, 21, and 28 days after irradiation. The specimen including the mandibular molars were sectioned and observed using a histopathological method. RESULTS: In the diabetes group, osteoclastic activity was observed in the alveolar bone and the root throughout the period of experiment. Also, osteoblastic and fibroblastic activities were markedly decreased. In the irradiation group, the osteoclasts were observed in the alveolar bone and the dilated capillaries were increased in the early experimental phases. However, vigorous osteoblastic activity was noted in the late experimental phases. In the diabetes-irradiation group, osteoclastic activity in the alveolar bone and the root was observed in the early experimental phases. However, there were no resorption and osteoblastic activity in the alveolar bone and the root in the late experimental phases, and obvious atrophic change of fibrous tissues was noted. CONCLUSION: This experiment suggests that osteoblastic activity was caused by irradiation in the late experimental phases, but atrophic change of the periodontal ligament tissues was induced after irradiation in diabetic state.
Animals ; Capillaries ; Citric Acid ; Diabetes Mellitus ; Fibroblasts ; Head ; Humans ; Male ; Molar ; Neck ; Osteoblasts ; Osteoclasts ; Periodontal Ligament ; Periodontium ; Rats* ; Rats, Sprague-Dawley ; Streptozocin

The purpose of this study was to established the characteristic radiographic features in salivary gland diseases by means of sialography and scintigraphy. Sialograms and scintigrams with diseases of salivary gland were examined. In this group were 5 salivary stones, 14 sialadenitis, 17 Sj gren's syndromes and 8 benign tumors. The obtained results were as follows; 1. In the configuration of the shape of main duct, those revealed that modified curvilinear and curvilinear types were predominant in Sj gren's syndromes but reverse sigmoid and angular types were in sialolithiasis and sialadenitis combined with sialodochitis. 2. In the configuration of the course of main duct, those revealed that smooth types were predominant in sialadenitis and irregular types were predominant in Sj gren's syndromes and benign tumors and irregular types were seen in all salivary stones and sialadenitis combined with sialodochitis, 3. In the type of intraglandular pattern, those revealed that destructive changes of salivary duct system and parenchyma were severe in sialadenitis and salivary stones and predominantly severe in Sj gren's syndromes. 4. The function of salivary gland was decreased severely in Sj gren's syndrome. and also decrease in salivary stone and sialadenitis. In benign tumor, the uptake of radioisotope was not seen in lesion and the function of salivary gland decreased in its remaining normal parenchyma.
Colon, Sigmoid ; Radionuclide Imaging ; Salivary Ducts ; Salivary Gland Calculi ; Salivary Gland Diseases ; Salivary Glands ; Sialadenitis ; Sialography*