Increased melanin pigmentation following ultraviolet irradiat.ion is due to increasing tyrosinase activity and multiplicatian of functioning melanocytes. After UV-irradiation, the size of melanocytes increases, and melanocyte dendrites elongatc, and branch. In this experiment, we induced the activation of melanocyts in the epidermis of C57BL black mice by ultraviolet-B(UVB) irradiation and observcd ihe effect of azelaic acid and retinoic acid on the UVB activated epidermal melanocytes. Sixty C57BL black mice were irradiated by UVB 100mJ/cm daily for 10 days, and then azeiaic acid and retinoic acid were topically applied daily for 7 weeks. For the estimation of morphologic change of epidermal melanocytes, light microscopic observation with split DOPA stain was performed at the end of the 1st, 3rd, 5th and 7th week of topical application. The results are summerized as follows : 1. The number, size and circumference of DOPA-positive epidermal melanocytes were significantly decreased in 20% azelaic acid applied group and 30% azelaic acid and 0.05% retinoic acid applied group. 2. In 20% azelaic;i.cid and 0.05% retinoic acid applied group, the number, size and circumference of DOPA-positive epidermal melanocytes were nore significantly decreased than in 20% azelaic acid applied group. In summary, the present study suggets that azelaic acid act as a depigmenting agent on epidermal melanocyte; and such depigmenting effect of azelaic acid was increased by addition of retinoic acid.
Animals ; Dendrites ; Dihydroxyphenylalanine ; Epidermis ; Melanins ; Melanocytes* ; Mice* ; Monophenol Monooxygenase ; Pigmentation ; Tretinoin*

No abstract available.
Acne Vulgaris* ; Rabbits*

Linear porokeratosis is a rare variant of porokeratosis and usually occurs in childhood. A 37-year-old female is presented with linear porokeratosis on the posterior aspeet of the right lower extremity exhibiting the classical histopathologic criteria of the disease. Treatment with an oral etretinate resulted in a remission of the lesions.
Acitretin ; Adult ; Etretinate ; Female ; Humans ; Lower Extremity ; Porokeratosis*

A case of "Follicular Occlusion Triad" associated with acne conglobata, hidradenitis suppurativa and perifolliculitis capitis abscedens et suffodiens in 40-year-old male patient is presented with brief review of literature. Widespread acne occured on the face k upper back at the age of 16. Several years later, firm & tender erythematous poorly demarcated nodules developed on the back, buttock and axilla. Also, erythematous nodules developed on temporooccipital area of the scalp. Thereafter, the skin lesions become fluctuant cystic. Some of the skin lesions healed leaving severe hypertrophic scars, and purulent materials expressed from some of persistent lesions. The patient was trested with systemic 13-cis-retinoic acid, antibiotics, corticosteroids injection and incision & drainage with considerable improvement.
Acne Vulgaris ; Adrenal Cortex Hormones ; Adult ; Anti-Bacterial Agents ; Axilla ; Buttocks ; Cicatrix, Hypertrophic ; Drainage ; Hidradenitis Suppurativa ; Humans ; Isotretinoin ; Male ; Scalp ; Skin

Comedones were induced at external ears of rabbits by daily application of 50% oleic acid in paraffin oil for 3 weeks. 13 cis-retinoic acid(RA) solution(0.1%)wae apllied to experimentally induced comedones. The morphologic changes around the epithelial lining of comedones were observed by transmission electron microscopy to define the effect of RA on comedones. In follicles treated with RA, the results were summarized as follows: l) The thickness of the stratum corneum of the epithelium was markedly decreased with widening of intercellular spaces. 2)The number of desomosomes and desmosoma1 bodies between horny cells was decreased. 3) Abnormally appeared tight junction in the stratum corneum and disappearance of membrane coating granules in the straurn granulosum were reversed to normal state, disappearance of tight junction and increment of membrane coating granule respectively by the application of RA. These observations suggest that RA has comedolytir. effect by decreasing cohesive- ness between horny cells.
Ear, External ; Epithelium ; Extracellular Space ; Membranes ; Microscopy, Electron, Transmission ; Oleic Acid ; Paraffin ; Rabbits ; Tight Junctions ; Tretinoin* ; Vitamin A* ; Vitamins*

We present two cases of angiosarcoma occuring on the scalp of aged person. The case 1 was a 74 year-old male who have had purplish colored, slightly raised irregular surfaced plsque on frontal scalp. The other case was a 69-year-old man who have had purplish nodule with satellite lesions on the right frontal area. The cases were confirmed by clinical picture, histologic findings. Treatment was not done because of refuse by patient.
Aged ; Hemangiosarcoma* ; Humans ; Male ; Scalp

At the time in vitro fertilization and embryo transfer, patients with unsuitable endometrium recovered by hormone. However, the overtreatment of hormone causes indispositionly the uterus internal secretion and finally induces endometriosis. Therefore, this study was done to inverstigate the effects of interleukin-2, which was known to differentiator and proliferator of T cells, on proliferation of the endometrial stromal cells in vitro. We have exammined the effects of interleukin-2, on the proliferation of bovine endometrial stromal cells in vitro, assessed by ['H]-thymidine incorporation and MTT assay methods. Results indicate that we isolated endometrial stromal cells from bovine uterus and established in vitro culture system. And interleukin-2 showed distint stimulatory effect on proliferation of the established stromal cells. These stimulative effects were not affected by estrogen and progesterone indirectly. In conclusion, these data imply that interleukin-2 may proliferate bovine endometrial stromal, cells, and it provides clue for understanding of direct actions of cytokines on the endometriat cells.
Cytokines ; Embryo Transfer ; Endometriosis ; Endometrium ; Estrogens ; Female ; Fertilization in Vitro ; Humans ; Interleukin-2 ; Interleukins* ; Progesterone ; Stromal Cells* ; T-Lymphocytes ; Uterus

The thioureylene drugs, propylthiouracil and methylmercaptoimidazol(MMI), exert their antithyroid effect primarily through inhibition of thyroid peroxidase-catalyzed iodination of thyroglobulin. Recently the interest about the effect to the thyroglobulin synthesis of these drugs have been increasing. So we studied the MMI effect to the thyroglobulin synthesis in cultured porcine thyroid cells. Porcine thyroid cells were isolated by sequential trypsinization in the presence of EGTA, seeded at high density(1X10^6 cells/cm^2) and cultured. One week later, MMI was added in different concentrations(0, 0.2, 1, 5mM) with TSH only or with 4H(b-TSH, Insulin, Transferrin, Hydrocortisone) or without hormone. Medias were collected after 24 hours and compared the amount of thyroglobulin secreted. And also pulse-labeling were performed with S^35 cysteine/methionine(1-2uCi/well) for 30, 60, 90min at the same conditions.There was no significant change in the amount of the secreted thyroglobulin by MMI, and there was no significant change in the pulse-labeled interacellular thyroglobulin by MMI. And also there was no significant change in the secretion of TSH-stimulated thyroglobulin by MMI. So we conclude that MMI has no effect on the thyroglobulin synthesis in cultured porcine thyroid cells and also MMI has no effect on the TSH-stimulated thyroglobulin synthesis in cultured porcine thyroid cell.
Antithyroid Agents ; Egtazic Acid ; Halogenation ; Insulin ; Methimazole ; Propylthiouracil ; Thyroglobulin ; Thyroid Gland ; Transferrin ; Trypsin

The amount of thyroglobulin synthesized from thyroid cells and stored in colloid space in very important in thyroid hormone synthesis. The thyroglobulin synthesis is mainly regulated by TSH secreted from the pituitary gland. But recently there were some reports about the possibility that iodine regulated the thyroid protein synthesis. So our studied were conducted to determined whether iodine could have inhibitory effect on thyroglobulin synthesis and methimazole could abolish the inhibitory effect of idoine.Porcine thyroid cells were isolated by sequential trypsinization in the presence of EGTA, seeded at high density(1X10^6 cells/cm^2) and cultured. One week later, Nal was added in different concentrations(10^-7, 10^-6, 10^-5, 10^-4M). 24hour medias were collected and checked the amount of thyroglobulin secreted. And also pulse-labeling were performed with[^35S] cysteine/methionine(1-2 uCi/well) for 1 hour at the same conditions. We used 3mM methimazole and 10^-4M NaI to observe the blocking effect of methimazol in iodine.The extracellular thyroglobulin secretion was significantly decreased by iodine in dose dependent manner(82.4%, 80.7%, 76.8% and 73.1% of control). And also intracellular thyroglobulin synthesis was significantly decreased by iodide in dose dependent manner(100.5%, 83.4%, 82.3% and 79.4% of control). The inhibitory effect of iodide was abolished by methimazole(74.7% to 101.3% of control). These data indicate that high iodide inhibit the thyroglobulin synthesis and secretion from the thyroid cells, and furthermore autoregulation by iodide may include thyroglobulin synthesis. And also this effect is dependent on the generation of an organic form of iodine because methimazole abolish the inhibitory effect of iodide.
Colloids ; Egtazic Acid ; Homeostasis ; Iodine ; Methimazole ; Pituitary Gland ; Thyroglobulin ; Thyroid Gland ; Trypsin

The Vogt-Koyanagi-Harada's syndrome (V-K-H) is chronic bilateral exudative uveitis associated with whitening of the hair and eye-lashes and various signs of meningeal iritation. Wereviewed some of the characteristic clinical findings of V-K-H syndrome as well as the histopathologic feature.
Hair ; Uveitis