OBJECTIVE: This study assessed the usefulness of the AutoPap 300 QC System in identifying of detection false negative(DFN) smears in a slide population previously screened as "within normal limits" and compared the rate of identification of DFN to that using 10% random selection. MATERIALS AND METHODS: From December 1996 to August 1998, we utilized the AutoPap 300 QC System at 30% selection rate to select cases for manual review. During the study period, 1,040(83.4%) among 1,247 smears and 7,848(84.0%) among 9,343 smears were screened as "within normal limits" and were included for the AutoPap 300 QC System and 10% random selection studies, respectively. RESULTS: 357(34.3%) among 1,040 were selected by the AutoPap 300 QC System at 30% QC selection rate and reviewed manually. Overall, 63 among 357 were determined to be abnormal including 47 ASCUS, 2 AGUS, and 14 LSIL. 785(10%) among 7,848 were selected by 10% random selection and reviewed manually. 96 among 785 were determined to be abnormal including 69 ASCUS, 3 AGUS, and 24 LSIL. These results represented an increase in pick up rate of DFN(detection false negative) of 5.1 and 4.4 fold for ASCUS/AGUS and LSIL, respectively and of 1.5 and 1.3 fold when accounting for the volume differences measured. CONCLUSIONS: The study demonstrated that the AutoPap 300 QC System is superior to 10% human random screening for the identification of DFN smears. Further studies are required using variable selection rates and a larger number of smears to fully assess the value of the device in quality control mode.
Humans ; Mass Screening ; Quality Control ; Vaginal Smears

No abstract available.

During ideal physiologic occlusion in the intercuspal position, all molars are occluded simultaneously and each occlusal contact evenly transmits similar pressure. The purpose of this study is to analyze the occlusion in the mandibular bone fracture patients treated by open reduction. This patients were selected according to the following criteria: 1) complete dentition without any missing teeth, 2) relatively normal occlusion without original TMJ disorder. Occlusal analysis using T-scan system were performed in 22 patients and 20 normal subjects. Our results were followed: 1. The number of occlusal contacts and the magnitude or occlusal force were increased with time. (p<0.05). 2. In the unilateral mandibular fracture patients, occlusal force and occlusal contacts were increased with time on the both sides. 3. There was significant difference in the number of occlusal contacts and occlusal force between postoperative 1 month and normal group(P<0.05), but there was no significant difference in the number of occlusal contacts and occlusal force between postoperative 6 month and normal group(p>0.05).
Bite Force ; Dentition ; Fractures, Bone ; Humans ; Mandibular Fractures* ; Molar ; Temporomandibular Joint Disorders ; Tooth

BACKGROUND: With the number of cancer survivors increasing, follow-up care to deal with problems related to their original cancer is needed. One of these is screening for a second primary cancer as cancer survivors are one of the high-risk groups for cancer occurrence. The purpose of this study was to assess the screening rates of major cancers in patients with a history of cancer in Korea. METHODS: Our data were from the 4th Korea National Health and Nutrition Examination Survey. We narrowed the data to include 11,169 adults aged 40 and 80 years. Participants were grouped according to their history of cancer and the time since cancer diagnosis into no cancer group (no cancer diagnosis), cancer survivor group (cancer diagnosis > or =5 years ago), and cancer follow-up group (cancer diagnosis <5 years ago). We estimated the screening acceptance rates of major cancers according to the cancer history and assessed the relationships between them. RESULTS: The cancer screening rates of stomach, breast, cervix, and colon were 42.4+/-4.9%, 45.5+/-5.6%, 42.1+/-6.4%, and 24.1+/-3.8% for the cancer survivor group and 45.6+/-5.2%, 61.9+/-6.2%, 48.8+/-7.0%, and 20.8+/-4.3% for the cancer follow-up group. A history of cancer diagnosis was not related to the acceptance rate of stomach and cervical cancer screening. Breast cancer screening (odd ratio [OR], 1.783; 95% confidence interval [CI], 1.044-3.048) increased only in the cancer follow-up group. At 5 years after a cancer diagnosis, only the screening rate for colon cancer (OR, 1.701; 95% CI, 1.119-2.588) persistently increased compared to individuals without a history of cancer. CONCLUSIONS: The screening for breast cancer was the only screening examination whose rate increased in the cancer follow-up group, with the significance disappearing in the cancer survivor group. Our results demonstrate that the screening rate for secondary cancers is below optimal in cancer patients in Korea.
Adult* ; Breast ; Breast Neoplasms ; Cervix Uteri ; Colon ; Colonic Neoplasms ; Diagnosis* ; Early Detection of Cancer ; Female ; Follow-Up Studies ; Humans ; Korea* ; Mass Screening* ; Neoplasms, Second Primary ; Nutrition Surveys ; Stomach ; Survivors ; Uterine Cervical Neoplasms

Treponema strain PFB4G is a novel oral spirochete and one of the most frequently detected organisms in subgingival plaque samples from rapidly progressive periodontitis and adult periodontitis patients. In this study, a genomic library of Treponema strain PFB4G was constructed in lambdaZAP expression vector. One positive clone that carried a 2.6-kb fragment was identified by screening with chicken Ig Y (immunoglobulin yolk) antibody raised against whole bacterial sonicates. Nucleotide sequencing of the subclones revealed an open reading frame (ORF) lacking the 5'-end. This region was obtained by PCR amplification using a degenerative and a specific primer. A complete open reading frame of 1,770 bp was identified and the deduced polypeptide consisted of 590 amino acids with a molecular mass of 65 kDa. The polypeptide, designated as OmpTL, had a typical prokaryotic signal sequence (19 amino acids) with a potential cleavage site for signal peptidase I and showed a significant level of homology with the outer membrane proteins of other oral treponemes, especially with that of Treponema maltophilum. The isolation of the gene encoding an outer membrane protein may allow the study of their roles in future, possibly as adhesion, pore forming or induction of proinflammatory cytokine synthesis.
Amino Acids ; Chickens ; Chronic Periodontitis ; Clone Cells* ; Cloning, Organism* ; Genomic Library ; Humans ; Mass Screening ; Membrane Proteins* ; Membranes* ; Open Reading Frames ; Periodontitis* ; Polymerase Chain Reaction ; Protein Sorting Signals ; Sequence Analysis* ; Spirochaetales ; Treponema*

No abstract available.
Hemorrhoids*

Thirty-nine healthy pigs (28-32 days old) were purchased from a commercial swine farm and housed at swine pens of the College. The animals were vaccinated intramuscularly (1 ml) with an attenuated live hog cholera virus (HCV, LOM strain) and then boostered at 5 weeks after the first vaccination. The animals were divided into 4 experimental groups: 0.05% (w/w) PowerFeel-supplemented diet (T-1, n = 10); 3% (w/w) SuperFeed-supplemented diet (T-2, n = 10); diluted PowerFeel solution (1 : 500, v/v) as drinking water (T-3, n=9); control (n=10). PowerFeel is an original form of ionized alkali mineral complex (IAMC) and SuperFeed is a commercial product of IAMC. The subpopulation of lymphocyte in blood was assayed by a flow cytometry and HCV-specific antibody was determined by an indirect immunofluorescence assay. In IMAC-treated groups, the proportions of subpopulation expressing MHC-class II, CD2+, CD4+, CD8+, and surface IgM+ B lymphocytes were significantly decreased at 5-weeks after the first vaccination. Significant decreases were also observed in the proportions of MHC-class II, CD2+ and CD8+ lymphocyte at 3-weeks after the booster injection. The humoral immune responses in T-1 and T-2 groups were greater than those in T-3 or control group. These results suggest that IAMC-supplemented diets may have an HCV-specific immunostimulatory effect in pigs.
Animal Feed ; Animals ; Antibodies, Monoclonal/*blood/isolation & purification ; Antigens, CD2/blood ; B-Lymphocytes/immunology ; CD4-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/immunology ; Classical Swine Fever/*immunology ; Classical swine fever virus/*immunology ; Dietary Supplements ; Ions ; Lymphocyte Subsets/*immunology ; *Minerals ; Swine ; Vaccines, Attenuated/*administration & dosage ; Viral Vaccines/*administration & dosage

No abstract available.
Hyperbilirubinemia, Neonatal*

The electrocardiographic and echocardiographic analysis was made on 23 patients with atrial septal defect of ostium secundum type, who were confirmed by right heart catheterization under admission at hanyang University Hospital from january 1979 to July 1981. Following results were obtained: 1) In the 23 patients, 13 were male and 10 female. Their ages ranged from 6 to 45 years, mean age being 19.9 years. 2) In 18 patients(78.3%), the QRS axis was between +90degrees and +150degrees. No significant correlations could be demonstrated between QRS axis and some hemodynamic parameters(right ventricular systolic pressure, pulmonary artery mean pressure and pulmonic to systemic flow ratio), and also, between QRS axis and the size of right ventricular dimension index. 3) Right ventricular outflow tract hypertrophy, right ventricular hypertrophy and complete right bundle branch block pattern in Lead V3R or V1were observed in 95.7% of ostium secundum defect. No significant correlations could be found between types of QRS complexes and some hemodynamic parameters(right ventricular systolic pressure, pulmonary artery mean pressure and pulmonic to systemic flow ratio), and also, between types of QRS complex and the size of right ventricular dimension index. 4) The mean amplitude of S wave in precordial Lead V6was 7.6mm. There was a definite correlation between the amplitude of S wave in Lead V6and right ventricular systolic pressure. No significant correlations could be demonstrated betwen the amplitude of s wave in Lead V6and some hemodynamic parameters(pulmonary artery mean pressure and pulmonic to systemic flow ratio), and also, the size of right ventricular dimension index. 5) Right ventricular dimension index was increased in all cases but one. A significant correlation was found between the size of right ventricular dimension index and pulmonary artery mean pressure, whereas no significant correlation could be demonstrated between the size of right ventricular dimension index and pulmonic to systemic flow ratio. 6) 95.7% of 23 patients with ostium secudum defect showed paradoxical septal motion was proved not to be associated with other congenital heart disease by cardiac catheterization. The pulmonic to systemic flow ratio in groups of septal motion type A was significantly increased than that of normal septal motion group, whereas no significantl relation between types of septal motion and the size of right ventricular dimension index was observed.
Arteries ; Axis, Cervical Vertebra ; Blood Pressure ; Bundle-Branch Block ; Cardiac Catheterization ; Cardiac Catheters ; Echocardiography* ; Electrocardiography* ; Female ; Heart Defects, Congenital ; Heart Septal Defects, Atrial* ; Hemodynamics ; Humans ; Hypertrophy ; Hypertrophy, Right Ventricular ; Male ; Pulmonary Artery

No abstract available.
Colorectal Neoplasms*