1.Expression of type IV collagen and laminin genes by skin fibroblasts in culture.
Korean Journal of Dermatology 1992;30(3):317-324
Basement membrane zone gene expression by fibroblast cultures was examined by molecular hybridizations with human sequence specific cDNAs corresponding to type 1V procollagen and laminin subunit polypeptides. Northern transfer analysis with total RNA revealed the presence of specific mRNA transcripts for al (IV) and a2 (1V) chains of type 1V procollagen as well as Bl and B2 chains of laminin. Laminin A chain mRNAs were not detected using the same RNA preparations. The molecular size of al (1V) and a2 (1V) procollagen mRNA revealed 6.8kb and 6.7kb, respectively. The molecular size of Bl chain of laminin revealed 5.6kb, and B2 chain revealed 8.2 and 5.5kb polymorphic transcripts. In slotblot analysis using densitometer, steady-state levels of type IV procollagen and laminin mRNAs indicated that they were in relatively low abunclance, as compaired with type I procollagen mRNA. Quantitative levels of al (IV) and laminin Bl chaii mRNAs were more abundant than those of a2 (IV) and laminin B2 mRNAs. The mRNA ratio of al (IV)/a2 (lV) and laminin Bl/B2 were 1.9 and 1.5, respectively. These results demonstrate evidence for differential regulation of the expression of different basement membrane zone molecules during the formation of basement membrane.
Basement Membrane
;
Collagen Type I
;
Collagen Type IV*
;
DNA, Complementary
;
Fibroblasts*
;
Gene Expression
;
Humans
;
Laminin*
;
Peptides
;
Procollagen
;
RNA
;
RNA, Messenger
;
Skin*
2.Multiple polyposis coli.
Jeong Heum BAEK ; Kyu Young JUN
Journal of the Korean Society of Coloproctology 1993;9(1):9-18
No abstract available.
Adenomatous Polyposis Coli*
3.The clinical reviews of the diverticular diseases.
Myung Kyun KIM ; Kyu Young JUN
Journal of the Korean Society of Coloproctology 1992;8(3):201-206
No abstract available.
4.Strangulated obturator hernia.
Young Seok PARK ; Yong Il KIM ; Kyu Young JUN
Journal of the Korean Surgical Society 1991;40(1):132-135
No abstract available.
Hernia, Obturator*
5.A clinical analysis of 311 cases of hemorrhoids.
Geon Seok LEE ; Sung Joon KWON ; Kyu Young JUN
Journal of the Korean Society of Coloproctology 1993;9(2):171-177
No abstract available.
Hemorrhoids*
6.Histopathological review of low anterior resection for rectal cancer.
Heung Woo LEE ; Sung Joon KWON ; Kyu Young JUN
Journal of the Korean Society of Coloproctology 1993;9(2):135-142
No abstract available.
Rectal Neoplasms*
7.A clinical analysis of 311 cases of hemorrhoids.
Geon Seok LEE ; Sung Joon KWON ; Kyu Young JUN
Journal of the Korean Society of Coloproctology 1993;9(2):171-177
No abstract available.
Hemorrhoids*
8.Histopathological review of low anterior resection for rectal cancer.
Heung Woo LEE ; Sung Joon KWON ; Kyu Young JUN
Journal of the Korean Society of Coloproctology 1993;9(2):135-142
No abstract available.
Rectal Neoplasms*
9.Hepatic resection for metastases from colorectal carcinoma.
Yu Gyo JUNG ; Kyu Young JUN ; Kwang Soo LEE
Journal of the Korean Society of Coloproctology 1993;9(2):97-106
No abstract available.
Colorectal Neoplasms*
;
Neoplasm Metastasis*
10.Comparative analysis of operative procedures in rectal prolapse between perineal and abdominal approach.
Ho Jin PARK ; Byung Jo SO ; Kyu Young JUN
Journal of the Korean Surgical Society 1991;40(3):354-359
No abstract available.
Rectal Prolapse*
;
Surgical Procedures, Operative*
Result Analysis
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