1.A new technique of corrective rhinoplasty for deflected bony dorsum.
Soon Jae YANG ; Kyu Yoon LEE ; Jong Sup PARK
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1992;19(5):847-854
No abstract available.
Rhinoplasty*
2.A clinical studies on Wilson's disease.
Won Kyu LEE ; Ki Sup CHUNG ; Chang Jun COE
Journal of the Korean Pediatric Society 1989;32(11):1496-1502
No abstract available.
Hepatolenticular Degeneration*
3.Correlation of Nitric Oxide Concentrations in Follicular Fluid with the Ovarian Response as well as the Age in Women Undergoing Controlled Ovarian Hyperstimulation.
Wook Hyun KWON ; Dong Hyung LEE ; Kyu Sup LEE
Korean Journal of Obstetrics and Gynecology 2005;48(4):996-1003
OBJECTIVE: To elucidate the correlation of nitric oxide (NO) concentrations in follicular fluid (FF) with the ovarian response as well as the age in women undergoing controlled ovarian hyperstimulation (COH). METHODS: This study was performed in 72 cycles of whom underwent COH for in vitro fertilization at infertility clinic of Changwon Hanmaeum Hospital and Pusan National University Hospital from Aug., 2003 to Dec., 2003. Each FF was collected at the oocyte retrieval and NO concentrations in FF were assessed. We analyzed the correlation of NO concentrations in FF with the ovarian response as well as the age in women undergoing COH. RESULTS: Among 72 cycles, the clinical pregnancy rate was 37.5% (27cycles). There were no significant differences in patient's age, ampules of gonadotropins used, estradiol level on the day of hCG administration, the number of oocytes retrieved, and fertilization rate between pregnant and nonpregnant groups. However, negative correlation was found between the follicular size and NO concentrations in FF. No significant correlation was found between age and NO concentrations in FF. Comparing NO concentrations in FF between pregnant and nonpregnant women by age, NO concentrations in FF were higher at nonpregnant women, significantly those of them more than 40 years old. CONCLUSION: These results suggest that NO concentrations in FF cannot be a marker for the ovarian response, and have no correlation with all parameters of COH including patients' age with exception for the follicular size.
Adult
;
Busan
;
Estradiol
;
Female
;
Fertilization
;
Fertilization in Vitro
;
Follicular Fluid*
;
Gonadotropins
;
Gyeongsangnam-do
;
Humans
;
Infertility
;
Nitric Oxide*
;
Oocyte Retrieval
;
Oocytes
;
Pregnancy Rate
4.Reconstruction of the lower leg with free latissimus dorsi myocutaneous flap.
Kyu Yoon LEE ; Yong Bae KIM ; Young Man LEE ; Soon Jae YANG ; Chong Sup PARK
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1991;18(1):101-106
No abstract available.
Leg*
;
Myocutaneous Flap*
;
Superficial Back Muscles*
5.Role of Leukemia Inhibitory Factor in the Effect of Co-Culture on Preimplantation Embryo Develpement.
Kyu Sup LEE ; Sang Woo KIM ; Yong Jin NA ; Young Ah LEE ; Ha Jung KIM ; Sung Kyu JANG
Korean Journal of Obstetrics and Gynecology 2000;43(7):1216-1222
OBJECTIVE: To assess the effect of recombinant human leukemia inhibitory factor on in vitro development of 1-cell ICR mouse embryo. MATERIALS AND METHOD: ICR mice were superovulated with PMSG/hCG and 1-cell stage mouse embryos were recruited. 1-cell mouse embryo were cocultured on human oviductal cells in a CO2 incubator (coculture group) and were cultured on 0.4% BSA+HTF media (control group). And anti-hLIF Ab was added the cocultured group in a different concentration (1pg, 10pg, 100pg, 1ng) and developmental rate was compaired to the control group, and rhLIF was added to the preincubated 0.4% BSA+HTF media in a different concentration (2000U, 1000U, 100U, 10U) and its developmental rate was compaired to group which was cultured on 0.4% BSA+HTF media only. RESULT: 1. The cleavage rate of 2-cell mouse embryo co-cultured with human tubal epithelial cell was significantly higher than that of cultured with media alone (HTF with 0.4% BSA) (p<0.05). 2. When LIF antibody was added to the medium with human tubal epitherlial cell, the mouse embryo could not cleave more than 2-cell in 1 ng of LIF antibody, and less than 1 ng, the cleavage rate was lower than cultured without LIF antibody group(p<0.05). 3. Two cell blocked ICR mouse embryos were developed into four cells under LIF(p<0.05), but no further development was observed. CONCLUSIONS: These results shows that LIF enhances the development of preimplantation embryo, and when rhLIF is applicated in vitro, it has positive effects on the development of early mouse embryo and can help overcoming the two-cell block.
Animals
;
Blastocyst*
;
Coculture Techniques*
;
Embryonic Structures
;
Epithelial Cells
;
Humans
;
Incubators
;
Leukemia Inhibitory Factor*
;
Leukemia*
;
Mice
;
Mice, Inbred ICR
;
Oviducts
6.A Case of Partial Trisomy of 3p (Trisomy of 3p23).
Seo Jeong KIM ; In Kyu KIM ; Kyu Hyoung LEE ; Sook Hwan LEE ; Kyoung Sup CHA ; Sun Ja PARK
Journal of the Korean Pediatric Society 1994;37(7):1006-1010
Partial Trisomy of 3p (Trisomy of 3p2, dup (3) (p23-->pter)) is a characteristic syndrome of chromosomal duplication of distal part of 3p, but breakpoints seem to vary in location. This syndrome shows multiple congenital anomalies with severe mental retardation, characteristic craniofacial change and absence of other gross external abnormalities. The craniofacial dysmorphism includes frontal bossing and temporal indentation, square face, marked hypertelorism, thick and short nose, full lips and a large mouth with downturned corners. Congenital heart defect, most frequently ASD and VSD, are found in most patients. In the majority of patients, the 3p2 duplication is the unbalanced product of a parental autosomal translocation involving 3p2 and another chromosome. We report a case of female baby who has facial dysmorphism, ASD and hyptonia and was found to have 3p2 duplidation (46XX-9, +der(9)t (3:9)(p23:p24)) by chromosomal analysis. Also we found her father was a carrier of blanced translocation of 3p2 and chromosome 9p (46XY, t(3:9)(p23:p24)).
Chromosome Duplication
;
Fathers
;
Female
;
Heart Defects, Congenital
;
Humans
;
Hypertelorism
;
Intellectual Disability
;
Lip
;
Mouth
;
Nose
;
Parents
;
Trisomy*
7.Application of Cumulus Cells as Factors to Predict the Outcome of IVF-ET.
Kwang Dae KIM ; Ki Hyung KIM ; Yong Jin NA ; Kyu Sup LEE
Korean Journal of Fertility and Sterility 1999;26(3):419-432
OBJECTIVE: To establish the evaluation system of the quality of oocytes on the basis of the incidence of cumulus cells apoptosis, to investigate the relationships between the incidence of cumulus cells and the outcomes of IVF-ET. METHOD: Thirth-four cycles undergoing controlled ovarian hypersimulation for IVF-ET with tubal infertility (23 cycles) or unexplained infertility (11 cycles) were included in this study. Cumulus cell masses surrounding mature oocyte and co-culture of embryos with autologous cumulus cells during IVF-ET process. The incidence of apoptosis in cumulus cells was assessed by apoptosis detection kit fluorescein. The effect of co-culture using cumulus cells and the incidence of cumulus cells apoptosis. RESULTS: The results were as follows: 1. The incidence of apoptosis in cumulus cells markedly increased in patients aged 40 or over, while the fertilization rate was greatly decreased in those age group. 2. Apoptosis in cumulus cells was found in both the fertilized oocytes and unfertilized oocytes, but the incidence of apoptosis was higher in unfertilized oocytes. 3. There is no clear correlation between apoptosis in cumulus cells and the number of oocytes retrieved. However, the incidence of apoptosis was increased when the number of oocytes retrieved was 5 and fewer in comparison with 6~10. 4. Embryo grade was significantly affected by the incidence of apoptosis in cumulus cells. 5. Pregnancy rate of IVF-ET per cycle was 29.4%, and the pregnant group had the higher fertilization rate and a significantly lower incidence of apoptosis in cumulus cells compared with the nonpregnant group. 6. When cumulus cells were used as helper cells in the co-culture of the embryo, in vitro activity of cumulus cells based on morphological change and proliferation did not influence the quality of embryo, but was closely associated with the implantation rate and pregnancy rate, which was enhanced when morphological changes and proliferation of cumulus cells was more active. 7. This difference in the outcome of IVF-ET according to in vitro activity of cumulus cells used for co-cultue was not associated with the incidence of apoptosis in cumulus cells, but rather had likely relations with the different secretion pattern of protein, which may be an embryotrophic factor by cumulus cells. CONCLUSION: These results suggest that the incidence of apoptosis in cumulus cells can be used in predicting oocyte qualities and the outcomes of IVF-ET. And the effect of co-culture largely depends on the in vitro activity of cumulus cells as well.
Apoptosis
;
Coculture Techniques
;
Cumulus Cells*
;
Embryonic Structures
;
Fertilization
;
Fluorescein
;
Humans
;
Incidence
;
Infertility
;
Oocytes
;
Pregnancy Rate
;
T-Lymphocytes, Helper-Inducer
8.Clinical and Cytogenetic Cytogenetic Studies in Primary Amenorrhea.
Ook Hwan CHOI ; Kyu Sup LEE ; Man Soo YOON ; Won Whe KIM
Korean Journal of Obstetrics and Gynecology 1998;41(11):2730-2738
The purpose of this study was to determine the frequency of chromosomal or genetic causes of primary amenorrhea, and was made to assess the etiology of disorders in those patients whose chromosome appeared normal. Sixty eight patients with primary amenorrhea were evaluated clinically and cytogenetically, which were refered to our Cytogenetic Laboratory in Department of Obstetrics and Gynecology, Pusan National University Hospital, from Aug. 1988 to Dec. 1996. The results were as follows. l. Out of 68 cases with primary amenorrhea, 40 cases (58.9%) had the normal chromosome constitutions and 28 cases (41.1%) had the abnormal chromosome constitutions including 46, XY. 2. Turner's syndrome was found in 25 cases (36.7%), consisting of 11 cases (16.1%) of 45, X, 3 cases (4.3%) of 46, X, i (Xq), 1 case (1.5%) of 46, X, inv (X), 1 case (1.5%) of 46, X, del (Xq), 1 case (1.5%) of 46, X, del (Xp), 1 case (1.5%) of 46, X, tel (Xq), 1 case (1.5%) of 45, X/46, XX, 1 case (1.5%) of 45, X/46, XY, 1 case (1.5%) of 45, X/47, XXX, 2 cases (2.9%) of 45, X/46, X, del (Xq), I case (1.5%) of 45, X/46, X, del (Xq), 1 case (1.5%) of 45, X/46, X, r (X). 3. 3 cases (4.3%) had the 46, XY chromosome constitution consisting of 2 cases (2.9%) of testicular feminization syndrome and 1 case (1.5%) of pure gonadal dysgenesis. 4. Among 40 patients whose chromosome are normal, the etiologies of amenorrhea were assumed to be caused by 11 cases (27.5%) of hypogonadotropic hypogonadism (idiopathic), 10 cases (25.0%) of congenital absence of vagina, 5 cases (12.5%) of pure gonadal dysgenesis in order of frequency.
Amenorrhea*
;
Androgen-Insensitivity Syndrome
;
Busan
;
Constitution and Bylaws
;
Cytogenetics*
;
Female
;
Gonadal Dysgenesis
;
Gynecology
;
Humans
;
Hypogonadism
;
Male
;
Obstetrics
;
Turner Syndrome
;
Vagina
9.A case of congenital intracranial teratoma.
Kwang Dae KIM ; Sul Mi KANG ; Yeoung Cheul CHOI ; Jae Gon CHO ; Kyu Sup LEE
Korean Journal of Obstetrics and Gynecology 1993;36(7):1632-1638
No abstract available.
Teratoma*
10.Cytologic Smear to Evaluate the Endocervical Canal after Cervical Conization.
Man Soo YOON ; Kyu Sup LEE ; Ook Hwan CHOI ; Won Whe KIM
Korean Journal of Obstetrics and Gynecology 2000;43(6):1002-1007
OBJECTIVE: To evaluate the impact of the cytologic smear of the endocervical canal immediately after cervical conization on the rate of residual cervical lesions. METHOD: A retrospective review of 229 patients who underwent cervical conization for high grade cervical intraepithelial neoplasia or microinvasive cancer followed by hysterectomy. Endocervical Papanicolaou smear with a cytologic brush performed immediately after cervical conization in all 229 patients. RESULTS: Fifty-four of 229 patients undergoing cervical conization followed by hysterectomy had residual lesion in the hysterectomy specimen. The prevalence rate of residual lesion according to the margin status of conization specimen as well as the results of the postconization Papanicolaou smear of the endocervical canal was compared. Forty-one of 78 patients (52.6 %) with positive margins had residual lesion compared with thirteen of 151 patients (8.6 %) with negative margins. Twenty-six of 34 patients (76.5 %) with positive postcone cytology had residual lesion compared with twenty-eight of 195 patients (14.4 %) with negative postcone cytology. CONCLUSIONS: Postcone cytologic smear of the endocervical canal as well as margin status are useful in predicting residual lesion after conization.
Cervical Intraepithelial Neoplasia
;
Cervix Uteri
;
Conization*
;
Female
;
Humans
;
Hysterectomy
;
Papanicolaou Test
;
Prevalence
;
Retrospective Studies