Melanosomes were isolated from the human hair by graded centrifugation and identified by transmission and scanning electron microscopic examination. Melanosomes were separated from the keratinous structures by treating with strong NaOH solution for 15 hours. The keratinous structures were removed by centrifugation ai 2,500xg and 3,500xg for 10 minutes respectively at 0 ℃. The isolated melanosomes were collected by centrifugation at 7,800xg at 0 ℃. Scanning electron microscopic examination made it possible to evaluate the global structure of purified melanosomes.
Centrifugation ; Hair* ; Humans* ; Melanosomes* ; Microscopy, Electron, Scanning

ERCP was performed in 38 cases of clonorchiasis of the liver, which was confirmed by stool egg test withStoll's dilution method from Apr. 1979 to Oct. 1982. The resuls were as follows 1. The sex distribution ofclonorchiasis showed marked male predominance by 97.4% and age distrigution were higher in 5th and 6th decades. 2.Multiple small filling defects in the intrahepatic bile duct was the most characteristic finding in ERCP and widespread irregular stenosis, dilatation of terminal portion, disturbance of peripheral filling, fuziness andraggedness of intrahepatic duct were also noted. 3. The severity of clonorchiasis by ERCP were classifed asfollows; Grade I-7 cases (18.4%), GradeII-9 cases(23.7%) Grade III-12 cases (31.6%) and Grade IV-10 cases(26.3%)4. Chronic cholecystitis 10 cases(26.3%), Billary tree stone 7 cases (18.4%), malignancy of distal CBD 1 case(2.6%) were found with clonorchiasis by ERCP. 5. Three cases, treated with Niclofolan, showed marked improvementof pathology on follow-up ERCP.
Bile Ducts, Intrahepatic ; Cholangiopancreatography, Endoscopic Retrograde ; Cholecystitis ; Clonorchiasis ; Constriction, Pathologic ; Dilatation ; Follow-Up Studies ; Humans ; Liver ; Male ; Methods ; Niclofolan ; Ovum ; Pathology ; Sex Distribution ; Trees

STUDY DESIGN: This is a retrospective study for radiographically and clinically assessing nonunion after lumbar spine fusion. OBJECTIVES: We wanted to analyze the risk factors for nonunion that requires surgical treatment after lumbar spine fusion SUMMARY OF THE LITERATURE REVIEW: A diagnosis of the nonunion after lumbar spine fusion was made by using only the only radiologic images. The incidence of nonunion has been underreported because there are many asymptomatic patients. MATERIALS AND METHODS: The plain X-ray films were evaluated for 1317 patients who could be followed up more than 1 year after lumbar fusion. Nonunion was diagnosed at 1 year after fusion by instability seen on the flexion-extension radiograph and the clinical findings like as sustained pain and local tenderness at the surgical site. The risk factors we reviewed included age, the number of levels fused, associated diseases, smoking, alcohol drinking, the initial diagnosis, a previous history of spinal operation, infection, a clear zone and malposition of pedicle screws and metal failure. The relations between nonunion and the factors mentioned above were analyzed. RESULTS: Thirty-nine patients were diagnosed as having nonunion underwent reoperation and all had surgically confirmed nonunion. Smoking, infection and a previous history of spine operation had a significant influence on nonunion (p < 0.05). Clear zones persisting more than 1 year and metal failure also had a significant influence on nonunion (p < 0.05). Age, the number of fused levels, the initial diagnosis and alcohol drinking were not shown to influence the rate of nonunion (p < 0.05). CONCLUSION: A through clinical and radiologic evaluation is essential to diagnose nonunion, and this should not be done according to the radiologic images only. Risk factors such as a previous history of spine operation, infection, smoking, the development of a clear zone and metal failure all showed a statistically significant influence on nonunion. Additionally, preoperative and postoperative evaluation of these parameters is needed to achieve bone union.
Alcohol Drinking ; Humans ; Incidence ; Reoperation ; Retrospective Studies ; Risk Factors ; Smoke ; Smoking ; Spine ; X-Ray Film

OBJECTIVE: The purpose of the current study was to evaluate survival outcome according to the expression status of CD73 in patients with epithelial ovarian cancer. METHODS: A total of 167 patients with epithelial ovarian cancer were enrolled in the current study. For each patient, a retrospective review of medical records was conducted. Immunohistochemical staining for CD73, CD8, FoxP3, and CD68 was performed using tissue microarray made with paraffin embedded tissue block. RESULTS: Among the enrolled patients, 29.9% of patients (n=50) showed negative expression for CD73, whereas 70.1% of patients (n=117) showed positive expression for CD73. The CD73 positive group showed better prognosis compared to the CD73 negative group (5-year overall survival of CD73 positive group, 73.0%; that of CD73 negative group, 50.1%; p=0.023). CD73 was more frequently expressed in mucinous adenocarcinoma and clear cell carcinoma compared to serous or endometrioid adenocarcinoma. In addition, CD73 overexpressions were more frequently detected in patients with known good prognostic factors, i.e., low stage, well/moderate differentiation, negative peritoneal cytology, no lymphovascular involvement, and no macroscopic residual tumor after debulking surgery. There was significantly more infiltration of regulatory T cells in the CD73 negative group compared to the CD73 positive group. CONCLUSION: Good prognosis in patients with overexpression of CD73 may be due to that overexpression of CD73 was more frequently observed in epithelial ovarian cancer patients with known good prognostic factors. Therefore, this result means that favorable differentiation and stage have more influence on survival outcome than adverse effect of CD73 per se.
5'-Nucleotidase ; Adenocarcinoma, Mucinous ; Carcinoma, Endometrioid ; Humans ; Medical Records ; Neoplasm, Residual ; Neoplasms, Glandular and Epithelial ; Ovarian Neoplasms ; Paraffin ; Prognosis ; Retrospective Studies ; T-Lymphocytes, Regulatory

OBJECTIVE: The purpose of the current study was to evaluate survival outcome according to the expression status of CD73 in patients with epithelial ovarian cancer. METHODS: A total of 167 patients with epithelial ovarian cancer were enrolled in the current study. For each patient, a retrospective review of medical records was conducted. Immunohistochemical staining for CD73, CD8, FoxP3, and CD68 was performed using tissue microarray made with paraffin embedded tissue block. RESULTS: Among the enrolled patients, 29.9% of patients (n=50) showed negative expression for CD73, whereas 70.1% of patients (n=117) showed positive expression for CD73. The CD73 positive group showed better prognosis compared to the CD73 negative group (5-year overall survival of CD73 positive group, 73.0%; that of CD73 negative group, 50.1%; p=0.023). CD73 was more frequently expressed in mucinous adenocarcinoma and clear cell carcinoma compared to serous or endometrioid adenocarcinoma. In addition, CD73 overexpressions were more frequently detected in patients with known good prognostic factors, i.e., low stage, well/moderate differentiation, negative peritoneal cytology, no lymphovascular involvement, and no macroscopic residual tumor after debulking surgery. There was significantly more infiltration of regulatory T cells in the CD73 negative group compared to the CD73 positive group. CONCLUSION: Good prognosis in patients with overexpression of CD73 may be due to that overexpression of CD73 was more frequently observed in epithelial ovarian cancer patients with known good prognostic factors. Therefore, this result means that favorable differentiation and stage have more influence on survival outcome than adverse effect of CD73 per se.
5'-Nucleotidase ; Adenocarcinoma, Mucinous ; Carcinoma, Endometrioid ; Humans ; Medical Records ; Neoplasm, Residual ; Neoplasms, Glandular and Epithelial ; Ovarian Neoplasms ; Paraffin ; Prognosis ; Retrospective Studies ; T-Lymphocytes, Regulatory

Three hundred renal transplantations were performed by the transplantation team in the Kosin Medical College from December, 1984 to August, 1993. Prognosis and complications with affecting factors and demographic data were analysed and the results were as follows; 1. In 300 recipients, 206 cases were male and 94 cases were female. 146 cases were male and 154 cases were female in 300 donors 2. All 300 cases were living transplantations ; among them, 207 cases were related and 93 cases were unrelated donors. 3. The most common underlying disease of recipients was chronic glomerulonephritis (89%). 4. In 263 donors, the left kidney was selected for graft. 5. For donor nephrectomy, flank incision( anterior incision) was used in all cases and among them 12th transcostal incision was made in 134 cases. 6. For ureteroneocystostomy, modified MacKinnon`s method was performed in 297 cases and Politano-Leadbetter procedure was used in only 3 cases. 7. Postoperative urologic complications occurred in 26 cases( 8.3%) ; perirenal hematoma, 16 cases for which revision was done; urine leakage, 8 cases for which 6 cases needed revision. 8. Complications of donor nephrectomy occurred in 8 cases( 2.6%) ; retroperitoneal hematoma, 2 cases; pneumothorax, 2 cases; pleural effusion, atelectasis, ulcer perforation and stress ulcer, one case respectively. 9. The survival rate for the graft was 91.6% for one year, 88.1 % for 2 years, 81.5% for 3 years, 77.7% for 4 years and 74.3% for 5 years. The survival rates of the recipients were 94.4%, 93.6%, 91.4%, 89.7% and 89.7% according to each year respectively.
Female ; Glomerulonephritis ; Hematoma ; Humans ; Kidney ; Kidney Transplantation* ; Male ; Nephrectomy ; Pleural Effusion ; Pneumothorax ; Prognosis ; Pulmonary Atelectasis ; Survival Rate ; Tissue Donors ; Transplants ; Ulcer ; Unrelated Donors

Sodium nitroprusside given by intravenous drip has been used to control hypertensive crises since 1929. Its evanescent action makes it suitable for controlled hypotension during general anesthesia with enflurane. The patients (n=10) were all undergoing Cotrel-Dubousset instrumentation for the operative treatment of scoliosis or spinal stenosis. Hemodynamic variables were obtained during administration of sodium nitroprusside. Mean arterial pressure was decreased to 50-60mmHg, while cardiac output, central venous pressure, systemic vascular resistance showed only minor changes. The average dosage of sodium nitroprusside (ug/kg/ min), plotted against age, showed a significant correlation (p<0.05, r= -0.63). The amonut of whole blood transfused during operation was 5.7+/-2.2 units (mean+/-SD) and postoperative hemoglobin and hematocrit (11.19+/-1.21g/dl, 33.49+/-3.79%, respectively, mean+/-SD) were singificantly lowered than preoperative hemoglobin and hematocrit (12.94+/-1.78g/dl, 37.22+/-4.88%, respectively, mean+/-SD) (hemoglobin: p<0.004, hematoorit: p<0.006).
Anesthesia, General* ; Arterial Pressure ; Cardiac Output ; Cardiovascular System* ; Central Venous Pressure ; Enflurane* ; Hematocrit ; Hemodynamics ; Humans ; Hypotension* ; Hypotension, Controlled ; Infusions, Intravenous ; Nitroprusside* ; Scoliosis ; Sodium* ; Spinal Stenosis ; Vascular Resistance

The muscle relaxants produce a dose-dependent effect that is usually mediated through an interaction between drug concentration in the vicinity of the receptors and receptor occupancy. Unfortunately, neither of these two variables is easy to measure the concentration of a relaxant drug at the neuromuscular junction, especeially in anesthetized humans, so indirect estimates must be made. Therefore, plasma concentration of a relaxant drugs may be taken as a reasonable estimate of the concentration at the effector site related directly to the dose given and of the degree of deporalization at the acetylcholine receptors occupancy. Immediately after the intravenous bolus injection of succinylcholine, there is almost instantaneous mixing into blood and penetrating into the neuromuscular junction and hydrolyzed rapidly by an enzyme within one or two circulation of succinylcholine in the body, and is so rapid that it is generally not detectable with conventional venous blood sampling within one or two minute intervals. This study conform the response of the time related plasma concentration of succinylcholine dose given by nerve stimulation. The twitch response of the adductor pollicis muslce was monitored using supramaximal TOF stimulation of the ulnar nerve at the left wrist by ABM (Datex Co.) and twitch response was recorded from release of tourniquet at 1, 2, 3 and 4 minutes after succinylcholine was given into the right arm after blood flow was occlude with tourniquet at left upper arm (study group) and from administration of succinylcholine without blood flow occlusion (control group). Conclusively the time duration from release of tourniquet 1, 2, 3 and 4 minutes after injection of succinylcholine to 75% T(1) recovery were 73.2%, 31.5%, 15.2% and 0% of the non-occluded arm respectively. Comparing with over 90% depression of twitch height until 4 minute after succinylcholine given, plama concentration at 4 minute after succinylcholine given (study group) did not make any significantly change of twitch height (p>0.05).
Arm ; Depression ; Humans ; Neuromuscular Junction ; Plasma ; Receptors, Cholinergic ; Succinylcholine* ; Tourniquets ; Ulnar Nerve ; Wrist

STATEMENT OF PROBLEM: The introduction of "The Long-term Care Insurance System"(a public nursing care insurance scheme) is scheduled from July in 2008. Lately, the importance of oral health care had increased. Introduction and establishment of the methodology (nursing care procedure) based on professional dental knowledge is inevitable. PURPOSE: The purpose of this study was to rouse recognition of the importance of dental care in the long term elderly care in nursing homes, with implementation of the new insurance law. MATERIAL AND METHODS: Visited two institutions for the elderly, Yudang Village and Sungjiwon located in Suwon city to investigate the present conditions in terms of (1) the detailsof the institution, (2) the activities concerning with dental care in the institution, and (3) the consciousness and recognition regarding dental care of the staffs. RESULTS: In two institutions, under the present conditions, oral cleaning (including the cleaning of denture) for residents was operated with no professional advices and limited professional dental care. It was found that there was very little awareness of aspiration pneumonia. The members of staff however, did recognizethe necessity of professional maintenance and management of oral care in daily nursing care, and that many residents hold dental problems. They were very eager in introducing the methodology (nursing care procedure) in dental care in nursing homes.
Aged ; Asian Continental Ancestry Group ; Consciousness ; Dental Care ; Dentists ; Humans ; Insurance ; Insurance, Long-Term Care ; Jurisprudence ; Korea ; Long-Term Care ; Nursing Care ; Nursing Homes ; Oral Health ; Pneumonia, Aspiration

To see the usefulness of CT in evaluation of response to treatment in patients with mediastinal tuberculous lymphadenitis (MTL), we analyzed the initial CT patterns and follow-up CTs or serial plain radiographs during 18 months antituberculous chemotheraphy in 58 consecutive patients. CT patterns of MTL at the beginning of treatment were categorized into solid type (n=8), low density with peripheral rim enhancement type (n=36), extranodal extension type (n=9) and calcified type (n=5). According to the response to treatment, each patients was categorized into prompt response group (response within the first three months and no residual lymph node after one year), slow response group (response after three months but residual lesion after one year), no response group (no change in size during 18 months treatment) and temporal increase group (temporal increase in size during the treatment but ultimately improved with chemotheraphy). Among 29 cases of prompt response group, 20 cases had large low-density areas, 6 cases had diffuse or extensive node involvement with extranodal extension or tracheal compression. Two patients with acquired immune derfciency syndrome belonged to this group. Fifteen cases of slow response group had low-density nedes in 7 patients and small solid or extranodal-extension nodes in 6 patients. They were associated with disseminated pulmonary tuberculosis, generalized lymphadenopathy or other organ(pericardium, brain, bone or abdomen) involvement. In 5 cases of no response group, there were 3 cases of calcified node and 2 cases of small solid nodes. Nine cases of temporal increase group included low density type of MTL only. And they were associated with disseminated pulmonary of endobronchial tuberculosis. In conclusion, response to antituberculous chemotherapy could be predicted in patients with MTL on the basis of CT findings before treatment.
Brain ; Drug Therapy ; Follow-Up Studies ; Humans ; Lymph Nodes ; Lymphatic Diseases ; Tuberculosis ; Tuberculosis, Lymph Node* ; Tuberculosis, Pulmonary