OBJECTIVES: lschemic acute renal failure(ARF) is characterized by an abrupt and sustained decline in GFR within minutes to days after renal ischemia and not immediately reversed on restoration of renal blood flow. The typical delay of a few days to a few weeks suggests reversible parenchymal damage awaiting cell regeneration for functional recovery. Many potentially cell damaging factors, such as ATP depletion, plasma membrane phospholipid degradatian and superoxide-induced membrane damage, play a central part in ischemic injury. More recently, much attention has been focused on the role of calcium, especially ischemic cell injury and the possible therapeutic role of calcium channel blockers emerged from studies conducted several years ago. In the past, it was thought that activation of renin-angiotensin system plays a role in the pathogenesis of ARF. Now the role of angiotensin in human renal ischemia also appears to be controversial. The following study was done in order to investigate the effect of a calcium channel blocker, nifedipine, on gene expression of renin during acute ischemic renal injury. METHODS: The Sprague-Dawley rats were divided into 4 groups, group I(n=3) as the control, group II (n=3) as the sham operation group, group III(n=15) as the ischemic renal injury group without nifedipine pretreatment, and group IV(n=15) as the ischemic renal injury model by right nephrectomy and left renal artery clamping for 40 minutes with systemic nifedipine pretreatment(10mg/kg), 1n ischemic renal injury model(group III and IV), rats were further divided into three subgroups according to reperfusion time of 1,24,72 hours. The non-ischemic right kidney removed at the time of initial procedure served as paired control. Total renal RNA was extracted by Chomczynskis method and electrophoresis was done in a 1% agarose gel containing 2,2M formaldehyde. Northern was performed at 42degrees C with isotope labeled renin probe for 18 hours, Autoradiographs were obtained and quantitated by a densitometer measured at 530nm. RESULTS: 1) The expression of renin gene was markedly decreased after renal ischemia and slowly recovered to one half of the control level after 72 hours of reperfusion. 2) Renin gene expression pattern of ischemic renal injury with prior nifedipine treatment was similar to the ischemic group without nifedipine pretreatment. CONCLUSION: These findings suggest that the renin gene expression was markedly decreased after renal ischemia and slowly recovered. Systemic nifedipine pretreatment does not have a significant effect on gene expression pattern of renin in ischemic renal injury.
Adenosine Triphosphate ; Angiotensins ; Animals ; Calcium Channel Blockers ; Calcium Channels* ; Calcium* ; Cell Membrane ; Constriction ; Electrophoresis ; Formaldehyde ; Gene Expression* ; Humans ; Ischemia ; Kidney ; Membranes ; Nephrectomy ; Nifedipine ; Rats ; Rats, Sprague-Dawley ; Regeneration ; Renal Artery ; Renal Circulation ; Renin* ; Renin-Angiotensin System ; Reperfusion ; RNA ; Sepharose

HPV infection has been implicated strongly in the pathogenesis of human squamous cell carcinoma(SCC). We analysed a series of 28 surgically removed, invasive squamous cell carcinoma of the esophagus by polymerase chain reaction to detect HPV DNA using consensus primers and 8 type-specific primers of HPV (6, 11, 16, 18, 31, 33, 35, 51). HPV 6, 31, 35 or 51 DNA were detected in 20 out of 28 cases (71.4%) of the esophageal SCCs. HPV 51 was the most frequently detected type, occuring in 13 out of 28 cases (46.4%). p53 immunohistochemical staining was also performed to demonstrate any relationship to HPV DNA positivity. It showed positivity in 16 out of 28(57.1%) esophageal SCCs, and HPV DNA and p53 positivity were concurrently detected in 11 out of 28 cases of SCCs. There was no significant inverse relation between HPV DNA positivity and p53 expression(p>0.05). Our results supported HPV involvement in esophageal squamous cell carcinoma, and suggested there may be another pathway not related to the p53-binding pathway in the carcinogenesis of esophageal SCCs by HPV.
Humans

No abstract available.
Fetus* ; Humans ; Pregnancy, Twin*

No abstract available.
Aorta, Thoracic* ; Blood Flow Velocity* ; Female ; Hypertension, Pregnancy-Induced* ; Pregnancy ; Umbilical Arteries*

No abstract available.
Dislocations* ; Talus*

There are some errors in the published article. The authors would like to make corrections in the original version of the article.

Henoch-Sch nlein purpura(HSP) occurs frequently in childhood and is a few occurrence in adults. The occurrence of nephrotic syndrome is rare in HSP patients and the association of renal disease with HSP is very important to prospect longterm clinical outcome. The causes of HSP have been diversely reported such as foods, drugs, sensitivity to infections, vaccination, insect bite, cold exposure etc and recently immune complex is studing as representative cause of HSP, but nothing is verified of definite mechanism of HSP until now. The occurrence of HSP associated with cancer has been rarely reported in the literature but there is no report in Korea. The patient was 31 year-old woman with stomach cancer who was operated in our hospital. 5 months after operation of stomach cancer, the massive proteinuria, generalized edema were developed and thereafter abdominal pain, generalized purpuric lesion on extremities were also occurred. We have done kidney biopsy and biopsy findings were compatible with HSP pathologically. After administration of prednisolone, spontaneous bacterial peritonitis was occurred suddenly, patient was recovered after ceasation of administration of prednisolone and proper antibiotic therapy but patient died of cachexia 3 months after diagnosis of HSP. We report a case of HSP presenting as nephrotic syndrome in patient with stomach cancer and complicated with spontaneous bacterial peritonitis.
Abdominal Pain ; Adult ; Antigen-Antibody Complex ; Biopsy ; Cachexia ; Diagnosis ; Edema ; Extremities ; Female ; Humans ; Insect Bites and Stings ; Kidney ; Korea ; Nephrotic Syndrome* ; Peritonitis* ; Prednisolone ; Proteinuria ; Purpura* ; Stomach Neoplasms* ; Stomach* ; Vaccination

Congenital M llerian anomalies of uterus are so infrequently encountered and endometriosis, primary infertility, hematometra, and urinary tract anomalies are claimed to be common complaints in women with unicornuate uterus. We report one case of rudimentary horn associated with agenesis of right kidney and pelvic endometriosis which is presented with brief review of literature about anomaly of the female genital tract.
Animals ; Endometriosis* ; Female ; Hematometra ; Horns* ; Humans ; Infertility ; Kidney* ; Urinary Tract ; Uterus

In order to investigate the fundus fundings in myopic eyes and to clarify the correlation between the refractive error and the myopic fundus changes, 7968 eyes of school children with refractive error in Seoul were selected and the observation of ophthalmoscopic features of crescent of optic nerve, cup to disc(C/D) ratio and the spontaneous venous pulsation were attempted. The result of the study were summarized as follows: 1. The optic nerve crescent was counted in 2643 eyes(33.2%) among 7968 eyes and the temporal crescent was the most frequent localization(72.8%) in 2643 eyes. 2. The incidence of the optic nerve crescent was increased in proportion to the degree of myopia regardless of its localization type, however the incidence of the crescent was decreased at -7.0 D or higher myopes. 3. The mean horizontal C/D ratio was 0.24 +/- 0.13 and the C/D ratio of 0.4 or more were common in the myopia of -2.0 D or more. The correlation between the C/D ratio and the refractive error appeared statistically significant(p<0.05). 4. In most of eyes(98.6%), difference of the C/D ratio between right and left eye was 0.2 or less. 5. The spontaneous venous pulsation on the optic disc was observed in 594 of 7968 eyes(7.5%).
Child ; Financial Management ; Humans ; Incidence ; Myopia ; Optic Nerve ; Refractive Errors* ; Seoul

Forty eight cases of strabismus were treated with an injection of Botulinum toxin A(Oculinum(R)) into the extraocular muscle under electromyographic control. There were 18 cases of esotropia, 14 of exotropia, 2 of hypertropia, 13 of unila teral abducens nerve palsy and 1 eyeball deviation even after the removal of old intraorbital metalic foreign body. All of the patients were followed up at least 6 months after the injection. By the final visit, the mean correction of preinjection deviation was 52.7% in esotropia, and 56.8% in exotropia, but there was no statistical significance between the two groups. Two patients of hypertropia obtained poor alignment chan ges after the injection. Among 13 patients with sixth nerve palsy, 10 who received injection only obtained mean 53.2% of correction and 3 patients who underwent both the injection and Jensen operation obtained 71.6%. In the one case of old intraorbital foreign body, lateral and inferior deviation before the injection were 60 P.D. and 20 P.D. respectively. After 5 months of injection, it improved to orthophoria and diplopia disappeared at primary position. The most common complication was ptosis, and it occurred in 19 patients(39.5%). Induced vertical deviation had the longest mean duration, lasting for 71.1 dyas. Twelve of 48 patients did not show any complications. No systemic complications were observed in any of the patients.
Abducens Nerve Diseases ; Botulinum Toxins* ; Diplopia ; Esotropia ; Exotropia ; Foreign Bodies ; Humans ; Strabismus*