To darify the cardiotoxic mechanism, the effect of bupivacaine on the cardiac function, especially in coronary flow rate was inveetigated in the isolated working rat heart. When administered into left atrium of the heart, lidocaine(10(-6)-10(-4) M) decreased heart rate in a dose-related fashion, whereae rate of coronary flow and mean coronary resistance tend to decrease and increase transiently, respectively. On the other hand, bupivacaine decreased the coronary flow rate and increased the mean coronary resistanee in a dose(10(-6)-10(-4) M) dependent manner. However, the decrement of heart rate by bupivacaine was not clear, but larger dose(10(-4) M) produced marked bradycardic effect. Bupivaeaine decreased the coronary flow and increased the mean coronary resistance in the isolated working heart, in which the heart rate and aortic pressure were kept constantly by electrical stimulation(3-6 pps, 0.5 mS, 20 V). These effects of bupivacaine were not influenced by 1 uM prazosin and 2 uM atropine pretreatment. But the bupivacaine effeets were completely abolished by 100 mM KC1 pretreatment and were inhibited markedly by 10(-7) M diltiazem, a Ca2+ -antagonist, pretreatment. From these results, it is suggested that the bupivacaine-induced coronary flow decrease is elicited via direct coronary vasoconstriction. And this vasoconstriction is due to the increments of intracellular Ca2+ concentration.
Animals ; Arterial Pressure ; Atropine ; Bupivacaine* ; Diltiazem ; Hand ; Heart Atria ; Heart Rate ; Heart* ; Prazosin ; Rats* ; Vasoconstriction

No abstract available.
Acute Kidney Injury* ; Nifedipine* ; Plasma* ; Renin*

We evaluated the effectiveness of combined extracapsular cataract extraction(ECCE), posterior chamber intraocullar lens implantation(PCIOL) and trabeculectomy with mitomycin C in patients with coexisting cataract and glaucoma. The charts were reviewed and compared of all patients who underwent combined procedure implantation without mitomycin C(group I) or with mitomycin C(group II) between 1990 and 1994 at Siloam Eye Hospital. A total of 44 eyes were included: 27 eyes in group I and 17 in group II. Intraocular pressure was controlled successfully in 66.6% of eyes in group I (mean IOP 15.9 +/- 6.6mmHg) and in 88.2% of eyes in group II (mean IOP 13.1 +/- 4.0mmHg). Fifteen of 27 eyes(55.6%) had a best corrected visual acuity of 20/40 or better in group I and thirteen of 17 eyes(76.5%) in group n at 6 months follow up. Complications included corneal haziness, posterior capsular opacity and encapsulated or flattened bleb in group I and shallow anterior chamber, hypotony, hyphema and bleb leakage in group II. The combined procedure with adjunctive mitomycin C appears to be a safe and effective in treating patients with coexisting cataract and glaucoma.
Anterior Chamber ; Blister ; Cataract Extraction* ; Cataract* ; Follow-Up Studies ; Glaucoma ; Humans ; Hyphema ; Intraocular Pressure ; Mitomycin* ; Trabeculectomy* ; Visual Acuity

This study was performed to determine whether peripapillary atrophy can be helpful in diagnosis of glaucoma and to investigate the correlation between peripapillary atrophy and optic disc head configuration in patients with primary open-angle glaucoma and normal-tension glaucoma. For all eyes, color polaroid optic disc photographs had been taken with Topcon retinal camera and reviewed. 93 eyes of normal subjects, 47 eyes of 47 patients with primary open-angle glaucoma and 47 eyes of 47 patients with normal-tension glaucoma were analyzed. Zone beta was detected more often in patients with normal-tension glaucoma and primary open-angle glaucoma (P<0.05). There was no significant difference in frequency of peripapillary atrophy between in patients with normal-tension glaucoma and primary open-angle glaucoma (P=0.071). In primary open-angle glaucoma,total,nasal and temporal neuroretinal rim area and total, inferior, nasal and temporal neuroretinal rim area/disc area ratio were smaller in patients with zone beta than without zone beta (P<0.05). The presence of zone beta was associated with neural tissue loss and progression of visual field in primary open-angle glaucoma (P<0.05). In conclusion,peripapillary atrophy, especially zone beta, is associated with glaucoma. Therefore, evaluation of peripapillary atrophy can be help-ful in diagnosing glaucoma. The presence of zone beta is associated with functional and structural optic nerve damage and is of predictive value in the future glaucomatous damage in primary open-angle glaucoma.
Atrophy* ; Diagnosis* ; Glaucoma ; Glaucoma, Open-Angle* ; Head ; Humans ; Optic Nerve ; Retinaldehyde ; Visual Fields

Dermatoglyphics has been studied in various diseases including mongolism, ruhella syndrome, congenital heart disease, selected neurologic diseases and other disorders. However, reports of derrnatoglyphic studies in patients with leprosy have not been evcountered a lot. Although Ieprasy is an infectious disease due to the hfycobacterium leprae, the hereditary susceptibility of the host to the organism is proposed as an additional predisposing factor. Thia concept has been proposed to reconcile the low incidence of lisease among large numbers of contacts. Most reports delving into the role of heredity in leprosy have been related ta epidemiological studies. Prasad and Mohamad suggest, on the basis of data related to rnultiple patient families, that the acquisition of leprosy may be determined genetically on the assumption of incomplete dominance of genes; on the other hand, Spickett suggested that if there is any genetic effect, it is but a component af the familial effect. This study of dennatoglyphics in 77 male leprosy patients was undcataken to determine if there might be significance in the analysis of dermal patterns af the hands as related to the hereditary susceptibility of individuals to the disease comparing with those of 234 healthy Korean soldiers. Results are as follows: 1. In the analysis of the total fingertip ridges, there is significant difference (P<0.01) between the leprosy (137.66+4.7) and the control group (152.93+9.50). 2. A aignificant statistical difference between the leprosy and the control group (14.73+2.79) does not exist with respect to the pattern intensity, however that af the borderline group of leprosy (12.00+5.09)is decreased (P<0.025). 3. In the incidence of simian lines, the leprosy group (5.20%) shows lower incidence rate than that of control group (8.98%). 4. As for the a-b ridge count, it is decreased significantly (P<0.05) in the left hand of leprosy group (34.71+0.87) than that of the eontrol group (37.11+0.52), Also there is significant difference(P<0. 05) in the right hands of tuberculoid type of leprosy (34.64+1.38). 5. With respect to the propoetional distance (mm.) between the distal flexion crease of the wrist to the base of the middle finger with the distance (mm.) of the same point of the wrist to the axial triradius, a significant statistical difference between the leprosy and the control group does not exist, but significantly longer distance (mm.) exists in the tuberculoid type of leprosy (P<0.05). 6. No significant fingertip pattern differences are noted between the leprosy and the control group.
Causality ; Communicable Diseases ; Dermatoglyphics* ; Down Syndrome ; Epidemiologic Studies ; Fingers ; Hand ; Heart Defects, Congenital ; Heredity ; Humans ; Incidence ; Korea* ; Leprosy* ; Male ; Military Personnel ; Wrist

PURPOSE: To demonstrate the characterization calretinin-immunoreactive displaced amacrine cells in the ganglion cell layer using immunohistochemistry and electron microscopy. METHODS: For immunohistochemistry, sections from guinea pig retina were incubated with mouse monoclonal antibody directed against calretinin. For double label studies, sections were incuated in mixture of mouse monoclonal anti-calretinin or rabbit polyclonal anti-calretinin with following antibodies: goat polyclonal anti-ChAT, rabbit polyclonal anti-GABA, mouse monoclonal anti-GABAA receptor alpha1, beta2/3. Sections were analyzed using Bio-rad Radiance Plus confocal scanning microscope. Stained sections from three guinea pig were observed with transmission electron microscope. RESULTS: Calretinin immunoreactivity was present in displaced amacrine cells and ganglion cells gaving rise to processes ramified in the inner part of the inner plexiform layer in stratum 4. The same stratum was also occupied by the dendrites of ON-cholinergic amacrine cells. Double-labeling demonstrated that dendrites and cell bodies of displaced amacrine cells colocalized with ON-cholinergic amacrine cells and dendrites of ganglion cells directly overlapped with dendrites of ON-cholinergic amacrine cells. The synaptic connectivity was identified by electron microscopy. Ganglion cell dendrites received synaptic input from ON-cholinergic amacrine cell. GABAA receptor beta2/3 subunit bands cofaciculates the dendrites of displaced amacrine cell and ganglion cell that are juxtapose to the alpha1 subunit of GABAA receptor. CONCLUSIONS: These results indicate that ON-cholinergic amacrine cells modulate calretinin-labeled ganglion cell via GABAA receptor beta2/3 in the guinea pig retina.
Amacrine Cells ; Animals ; Calcium-Binding Protein, Vitamin D-Dependent ; Dendrites ; Electrons ; Ganglion Cysts ; Goats ; Guinea ; Guinea Pigs ; Immunohistochemistry ; Mice ; Microscopy, Electron ; Retina

Fourty-one glaucoma triple surgeries(Group I: combined trabeculectomy, planned extracapsular cataract extraction(P-ECCE) 27 cases and Group II: phacoemulsification and posterior chamber intraocular lens(PC IOL) implantation 14 cases) were reviewed. Preoperatively, the two groups did not differ significantly in terms of age, sex, or mean intraocular pressure(IOP). As comparaed with Group I, the Group II had earlier visual rehabilitation, and less postoperative IOP spikes(P<0.05), and needed less postoperative antiglaucoma medications. Other postoperative complications occurred more commonly in the Group I: fibrinous iritis, posterior capsular opacity, encapsulated or flattened bleb, uncontrolled IOP etc. Compared to group II, these differences probably are attitutable primarily to the small incision used in phacoemulsification, which is associated with sustained anterior chamber depth during the operation, and to the reduction of tissue dissection, reducing the stimulus to wound healing and filtration failure.
Anterior Chamber ; Blister ; Cataract Extraction* ; Cataract* ; Fibrin ; Filtration ; Glaucoma* ; Humans ; Iritis ; Phacoemulsification* ; Postoperative Complications ; Rehabilitation ; Trabeculectomy ; Wound Healing

The Nerve Fiber Layer Anlyzer (LDT Inc., U.S.A.) is a scanning laser polarimetric ophthalmoscopy that uses the polarizing properties of the retinal nerve fiber layer. I performed 10 consecutive measurements from 15degrees peripapillary area in 10 normal eyes and 10 glaucomatous eyes to evaluate the reproducibility of the instrument. The nerve fiber layer thickness was are 76.8+/-9.0m for normal subjects and 81.5+/-8.4m for galucoma patients. The mean coeffitient of variation of the 1.5 disc diameters (15degrees) for normal subjects was 11.6(3.14-11.27) and for glaucoma eyes was 10.4(2.4612.46). This results indicate that the reproducibility of NFA was good in measuring the retinal nerve fiber layer thickness. Further investigation seems warranted to compare normal and glaucomatous eyes.
Glaucoma ; Humans ; Nerve Fibers* ; Ophthalmoscopy ; Retinaldehyde*

The author has measured refractive error and corneal curvature in 500 eyes of 250 patients who visited the Department of Ophthalmology, Ewha Womans University Hospital, with decreased visual acuity as chief complaint, for 6 months from Sep. 1, 1984 through Feb. 28, 1985, and found the following conclusions: First, as for the means of each corneal horizontal curvature and vertical curvature, the mean of horizontal curvature was 7.7876 +/- 0.9791mm; that of vertical curvature was 7.7217 +/- 0.8527mm in right cornera, while the mean of horizontal curvature was 7.8628 +/- 0.7295mm; that of vertical curvature was 7.5986 +/- 1.2349mm in the left cornea. Second, as for the comparison of corneal curvature by sex, it was highly significant(p<0.05) that the means of each corneal horizontal curvature and vertical curvature were always larger in the case of male than that of female. Third, as for the comparison by each age group(age groups under 25 years, from 25 through 44 years, and from 45 years up respectively), there were no significant differences(0 0.05) in respective means of each corneal horizontal curvature and vertical curvature among age groups. Fourth, as the result of this study on correlation between corneal curvature and refractive error, its correlation is 0.32% to 4.66%, showing no significant correlation between them.
Cornea ; Female ; Humans ; Male ; Ophthalmology ; Refractive Errors* ; Visual Acuity

PURPOSE: To evaluate the difference of retinal nerve fiber layer measurements as obtained by Nerve Fiber Analyser according to visual field defects in subjects with large Cup-to-Disc ratio (C/D ratio>or=0.6) and to determine which predictable parameters are useful in glaucoma diagnosis. METHODS: Fifty one normal subjects, 17 patients with glaucoma, 18 glaucoma suspects based on optic disc appearance and visual field defect participated. Nerve fiber layer thickness assessments using scanning laser polarimeter (Nerve Fiber Analyzer II software version 2.1.; Laser Diagnostic Technologies, Inc., San Diego, California, U.S.A.) were measured in normal subjects, patients with glaucoma, and glaucoma suspects. Measured nerve fiber layer thickness parameters were compared each other. RESULTS: Superior to inferior ratio (S/I ratio) was only statistically significant parameter to differentiate glaucoma from glaucoma suspects having glaucomatous optic nerve head. CONCLUSIONS: Superior to inferior ratio (S/I ratio) is useful parameter to predict differentiating patients with glaucoma from glaucoma suspects.
California ; Diagnosis ; Glaucoma* ; Humans ; Nerve Fibers* ; Optic Disk ; Retinaldehyde* ; Scanning Laser Polarimetry ; Visual Fields