1.Translocation of p53 Protein in Melanocytes and Malignant Melanoma Cells After UVB Irradiation.
Kyoung Hee CHOI ; Hye Jin KIM ; Yong CHOI ; Kyu Han KIM ; Kyoung Chan PARK
Annals of Dermatology 2001;13(3):133-138
BACKGROUND: UVB is responsible for most of the carcinogenic effects of sun exposure. OBJECTIVE: Aim of this study was to investigate the regulation and intracellular redistribution of p53 protein after UVB irradiation. METHODS: Cultured normal human melanocytes and G361 melanoma cells were used for west-ern blotting and confocal microscopic examination for determining expression and distribution of p53. RESULTS: UVB irradiation increased p53 expression in cultured normal human melanocytes and G361 malignant melanoma cells according to increasing doses of UVB. Furthermore, p53 moved from cytosol to nucleus after UVB irradiation. CONCLUSIONS: UVB irradiation induced overexpression and redistribution of p53 in cultured normal human melanocytes and G361 malignant melanoma cells.
Cytosol
;
Humans
;
Melanocytes*
;
Melanoma*
;
Solar System
2.Establishment of Embryonic Stem Cell Line from Isolated Blastomeres from Mouse Preimplantation Embryos.
Chun Kyu LIM ; Ji Hye SUNG ; Hye Won CHOI ; Jae Won CHO ; Mi Ra SHIN ; Jin Hyun JUN
Korean Journal of Fertility and Sterility 2006;33(1):25-34
OBJECTIVE: The aim of this study was to investigate whether embryonic stem (ES) cells can be established from isolated blastomeres of mouse embryos. METHODS: Blastomeres were separated from mouse (C57Bl/6J) 2- or 4-cell embryos. Isolated blastomeres or whole 4-cell embryos were co-cultured with mitosis-arrested STO feeder cells in DMEM supplemented with recombinant murine leukemia inhibitory factor and ES-qualified fetal bovine serum. After the tentative ES cell lines were maintained from isolated blastomeres or whole embryos, some of them were frozen and the others were sub-cultured continually. Characteristics of tentative ES cell lines as were evaluated for specific gene expressions with immunocytochemistry and RT-PCR. RESULTS: One ES cell line (3.0%) was established from isolated blastomere of 2-cell embryo and one cell line (4.0%) from isolated two blastomeres of 4-cell embryo. And five cell lines (16.7%) were established from whole 4-cell embryos. Both cell lines from isolated blastomere and whole embryo expressed mouse ES cells specific markers such as SSEA-1, Oct-4 and alkaline phosphatase. Marker genes of three germ layers were expressed from embryoid bodies of both cell lines. CONCLUSION: This study suggests that mouse ES cells could be established from isolated blastomeres, although the efficiency is lower than whole embryos. This animal model could be applied to establishment of autologous human ES cells from biopsied blastomeres of preimplantation embryos in human IVF-ET program.
Alkaline Phosphatase
;
Animals
;
Antigens, CD15
;
Blastocyst*
;
Blastomeres*
;
Cell Line
;
Embryoid Bodies
;
Embryonic Stem Cells*
;
Embryonic Structures
;
Feeder Cells
;
Gene Expression
;
Germ Layers
;
Humans
;
Immunohistochemistry
;
Leukemia Inhibitory Factor
;
Mice*
;
Models, Animal
3.A Case of Inflammatory Pseudotumor of the Urinary Biadder.
Hye Rim PARK ; Min Chul LEE ; Nack Kyu CHOI ; Young Euy PARK
Korean Journal of Pathology 1991;25(3):256-262
Inflammatory pseudotumor of the urinary bladder is a proliferative spindle cell lesion that microscopically may suggest a sarcoma but that are benign without a recent history of an operation. The first such case was reported by Roth, in 1980, and thereafter about seven more cases were reported in medical literatures. We reported a case of inflammatory pseudotumor of the urinary bladder mimicking leiomyosarcoma. Patient was a 36-year-old woman with complaint of painless total and gross hematuria for 3 weeks. Partial cystectomy specimen showed a well-demarcated nodular mass of yellow white color, involving the submucosal and muscular layers. Microscopic examination revealed proliferating bundles of spindle cells interspersed with infiltration of many inflammatory cells including eosinophils. Spindle cells were positive for vimentin on immunohistochemistry and corresponding to myofibroblasts on the electron microscopic examination.
Female
;
Humans
4.A Case of Spinal Cord Astrocytoma Associated with Hemorrhage.
Hyung Chun PARK ; Hye Young CHOI ; Kyu Man SHIN ; Seoung Yon BAEK ; Sun Wha LEE
Journal of the Korean Radiological Society 1994;31(3):411-414
Magnetic Resonance image(MRI) is used as the diagnostic modality for evaluation of suspected intramedullary tumors and differential diagnosis of these tumors at the spinal cord. We experienced intramedullary astrocytoma of cerviced cord with large syrinx and multiple peritumoral cysts consisted of subacute and chronic hemorrhage at the margin and within the syrinx and cysts on MRI.
Astrocytoma*
;
Diagnosis, Differential
;
Hemorrhage*
;
Magnetic Resonance Imaging
;
Spinal Cord*
5.MRI Evaluation of the Vascular Pattern in Intracranial Meningioma.
Mi Hye KIM ; Kyung Sub SHINN ; Hyo Sun CHOI ; Kyu Ho CHOI ; Il Gwon YANG ; Han Jin LEE
Journal of the Korean Radiological Society 1994;30(2):225-229
PURPOSE: The purpose of this study was to assess the usefulness of MRI in the evaluation of vascular changes of the meningioma. MATERIALS AND METHODS: We retrospectively studied the MRI findings in 20 cases of intracranial meningiomas. The images were assessed for vascular hilum, vascular rim, sinus invasion, and vascular encasement. Cerebral angiograms were obtained in 15 cases and compared with MRI findings. RESULTS: MRI was as accurate as angiography in detection of vascular hilum(MRl=8/20, Anglogram=9/15), vascular rim(MRl=14/20, Angiogram=9/15) and dural sinus invasion(MRl=4/6, Angiogram=5/6). MRI was superior to angiography in detection of vascular encasement(MRl=2/3, Angiogram=0/2) and cavernous sinus invasion(MRI=3/3, Angiogram=0/2). CONCLUSION: MRI is an excellent noninvasive imaging modality in evaluating vascular changes of omas.
Angiography
;
Cavernous Sinus
;
Magnetic Resonance Imaging*
;
Meningioma
;
Retrospective Studies
6.The accuracy of computed tomography in the staging of carcinoma of the uterine cervix.
Kyu Young LEE ; Ckan Kyu PARK ; Chan PARK ; Hye Ree SUNG ; Nam Jong CHOI ; Jae Wook KIM ; Dong Hee CHOI ; Su Nyung KIM
Korean Journal of Obstetrics and Gynecology 1993;36(7):1120-1126
No abstract available.
Cervix Uteri*
;
Female
7.Imaging Findings of Osler-Weber-Rendu Disease Involving the Liver.
Dong Ho LEE ; Jin Wook CHUNG ; Jae Hyung PARK ; In Kyu YU ; Hye Kyung YOON ; Guk Myeong CHOI
Journal of the Korean Radiological Society 1995;32(6):943-946
A 62-year-old woman with hereditary hemorrhagic telangiectasia(HHT) or Osler-Weber-Rendu disease involving the liver is presented. Imaging findings including color Doppler sonograph and CT findings are described.
Female
;
Humans
;
Liver*
;
Middle Aged
8.Anterior clinoid process and optic strut in Koreans.
Hye Yeon LEE ; In Hyuk CHUNG ; Byoung Young CHOI ; Kyu Sung LEE
Yonsei Medical Journal 1997;38(3):151-154
The anterior clinoid process and the optic strut are often removed during operation on the anterior part of the cavernous sinus. Therefore it is important for neurosurgeons to verify their dimensions and variations. The purpose of this study was to investigate the dimension and the variation of the anterior clinoid process and to describe the locational variation of the optic strut. Seventy-three skulls of Korean adults were used. The average length, basal width and thickness of the anterior clinoid process were 9.18 +/- 1.55, 9.63 +/- 1.49 and 5.32 +/- 1.07 mm, respectively. The average thickness of the optic strut was 2.9 +/- 1.15 mm and it was commonly attached to anterior two-fifths of the anterior clinoid process. The complete caroticoclinoid canal was observed in 4.1%, however it was incomplete in 11.6%. The incidence of a caroticoclinoid canal in Koreans was relatively low compared with other races.
Adult
;
Cadaver
;
Cavernous Sinus/anatomy & histology*
;
Human
;
Korea/ethnology
;
Mongoloid Race*
;
Sphenoid Bone/anatomy & histology*
9.Reliability of the Single Cell PCR analysis for Preimplantation Genetic Diagnosis of Single Gene Disorders.
Hye Won CHOI ; Hyoung Song LEE ; Chun Kyu LIM ; Mi Kyoung KOONG ; Inn Soo KANG ; Jin Hyun JUN
Korean Journal of Fertility and Sterility 2005;32(4):293-300
No abstract available.
Epidermolysis Bullosa
;
Muscular Dystrophy, Duchenne
;
Ornithine Carbamoyltransferase
;
Polymerase Chain Reaction*
;
Preimplantation Diagnosis*
10.Clinical Value of New Staging Systems for Multiple Myeloma.
Jung Hye CHOI ; Jae Hoon YOON ; Seong Kyu YANG
Cancer Research and Treatment 2007;39(4):171-174
PURPOSE: We wanted to investigate the validity of the recently introduced Southwest Oncology Group (SWOG) staging system and the International Staging System (ISS) by comparing both systems with the widely accepted Durie/Salmon (DS) system for multiple myeloma patients. MATERIALS AND METHODS: Between 1992 and 2005, 85 multiple myeloma patients (men: women 41:44, median age: 63 years (range: 36~87)) with available baseline values of albumin and beta2-microglobulin were enrolled. The clinical and laboratory data were retrospectively obtained. RESULTS: According to the ISS, 11 patients were stage I (12.9%), 30 patients stage II (35.3%) and 44 patients stage III (51.8%). The median survivals of the ISS stages I, II and III were 78.6 months, 31.8 months and 15.1 months, respectively (p=0.015). The DS staging system was not able to predict the survival. For the SWOG staging system, 14 patients were stage I (16.4%), 27 patients stage II (31.8%), 27 patients stage III (31.8%) and 17 patients were stage IV (20.0%). The median survivals of the SWOG staging system stage I, II, III and IV were 78.6 months, 31.8 months, 11.6 months and 24.8 months, respectively (p=0.0075). CONCLUSION: The ISS staging system showed better reliability, simplicity and predictability for survival than the DS and SWOG staging systems for multiple myeloma patients.
Female
;
Humans
;
Multiple Myeloma*
;
Retrospective Studies