Sixty cutaneous tumors of different types were investigated by means of immunoperoxidase technique(avidin-biotm-peroxidase complex method) with the use of antikeratin antibody. The results were as follows: 1. Squamous cell carcinomas, keratoacanthomas, and Bowen's disease exhibited strong reactivity with antikeratin antibody. 2. In squamous cell carcinomas and keratoacanthomas, the most heaviest staining patterns were detected in areas of keratinization and horn pearl formation. 3. Basal cell carcinomas and seborrheic keratosis were moderately stained, whereas Paget'a disease and metastatic carcinoma(oat cell carcinoma) did not contain keratin. 4. In some cases of basal cell carcinomas, the tumor cells were partially positive. 5. Malignant melanomas, nevi, malignant.lymphomas, neural tumors, hemangiomas, and dermatofibromas were uniformly negative.
Animals ; Bowen's Disease ; Carcinoma, Basal Cell ; Carcinoma, Squamous Cell ; Hemangioma ; Histiocytoma, Benign Fibrous ; Horns ; Keratoacanthoma ; Keratosis, Seborrheic ; Melanoma ; Nevus

No abstract available.
Acanthosis Nigricans* ; Vitiligo*

BACKGROUND: Amniotic membrane contains basement membrane components and several growth factor proteins. Therefore, transplantation of amniotic membrane might help epithelialization. OBJECTIVE: The purpose of this study was to compare efficacies of amniotic membrane patches (amniotic group), polyurethane film (TegadermTM, 3M, USA: wet group), and nonadherent dressing (dry group) in wound healing. METHOD: Six rabbits were included in this study. We made wound defects on the back of each rabbit. Amniotic membrane patches (amniotic group) was applied to each wound defect in the first group, polyurethane film (TegadermTM, 3M, USA: wet group) to the second group, and nonadherent dressings (dry group) to the third group. We compared the time taken for each wound defect to heal, and the gross and histopathologic change of the wound defect from baseline, over time. RESULTS: The results were as follows: 1. In the early phase, the amniotic membrane patch (amniotic group) resulted in a faster healing time than either the polyurethane film (TegadermTM, 3M, USA: wet group) or the nonadherent dressing (dry group). 2. On day 10, all groups achieved histological epithelialization. 3. In the late phase, the amniotic membrane patch (amniotic group) might inhibit collagen fiber arrangement and development of skin appendages. CONCLUSION: The amniotic membrane patch is effective in the treatment of rabbit wound defects. It can serve as a biological dressing in the early phase of wound defects.
Amnion* ; Bandages ; Basement Membrane ; Biological Dressings ; Collagen ; Polyurethanes ; Rabbits ; Skin* ; Wound Healing* ; Wounds and Injuries*

Palisaded encapsulated neuroma (PEN) is a clinically-distinctive, benign, cutaneous nerve sheath tumor. It usually occurs as a solitary, asymptomatic, skin-colored papule, and commonly affects the face of middle-aged adults. We report a case of PEN which developed on the lower lip of a 44-year-old Korean man. It was a 5mm-sized skin colored papule. Histopathologically, it consisted of uniform, broad, interlacing fascicles of spindle cells and was surrounded by a complete capsule in the dermis. On immunohistochemical staining, the tumor cells of the nodule were positive for S-100 protein and neural filaments, whil the capsule of the nodule was positive for epithelial antigen.
Adult ; Dermis ; Humans ; Lip* ; Neuroma* ; S100 Proteins ; Skin

In addition to cellular damage, ischemia-reperfusion (IR) injury induces substantial damage to the mitochondria and endoplasmic reticulum. In this study, we sought to determine whether impaired mitochondrial function owing to IR could be restored by transplanting mitochondria into the heart under ex vivo IR states. Additionally, we aimed to provide preliminary results to inform therapeutic options for ischemic heart disease (IHD). Healthy mitochondria isolated from autologous gluteus maximus muscle were transplanted into the hearts of Sprague–Dawley rats damaged by IR using the Langendorff system, and the heart rate and oxygen consumption capacity of the mitochondria were measured to confirm whether heart function was restored. In addition, relative expression levels were measured to identify the genes related to IR injury. Mitochondrial oxygen consumption capacity was found to be lower in the IR group than in the group that underwent mitochondrial transplantation after IR injury (p < 0.05), and the control group showed a tendency toward increased oxygen consumption capacity compared with the IR group. Among the genes related to fatty acid metabolism, Cpt1b (p < 0.05) and Fads1 (p < 0.01) showed significant expression in the following order: IR group, IR + transplantation group, and control group. These results suggest that mitochondrial transplantation protects the heart from IR damage and may be feasible as a therapeutic option for IHD.