1.Cytokine production of peripheral blood mononuclear cells from atopic asthmatics.
Bin YOO ; Jae Kyoung PARK ; Hee Bom MOON ; Jeong Yeon SHIM ; Soo Jong HONG ; Yoo Sook CHO
Korean Journal of Allergy 1997;17(3):307-315
To investigate the imbalance of the cytokine production profile of T cells from atopic asthmatics, we measured concentrations of IL-4, IL-5 and IFN-y by ELISA method in the culture supernatants of peripheral blood mononuclear cells(PBMCs) and Derrnato-phagoides pteronyssinus(Der p) J-stimulated PBMCs from Der p-sensitized atopic asthmatics, Der p-sensitized healthy atopits, non-atopic asthmatics and healthy non-atopics. The suppressive effect of IFN-y on cytokine production of Der p J-stimulated PBMCs was also examined. The PBMCs from atopics showed higher IL-4 and IL-5 production in response to PHA +TPA and higher IFN-gamma production in response to Der p Jq compared with non-atopits. The Der p J-stimulated PBMCs from atopics showed a tendency of increased IL-5 production in response to Der p J and higher IL-4 and IL-5 production in response to PHA+TPA compared with non-atopics. IL-5 production of Der p J-stimulated PBMCs from atopics was suppressed by IFN It is suggested that an imbalance in IL-4, IL-5 and IFN-y production is a feature of the atopic state. The TH2 characteristics of allergen-stimulated PBMCs could be regulated by IFN-y.
Enzyme-Linked Immunosorbent Assay
;
Interleukin-4
;
Interleukin-5
;
T-Lymphocytes
2.A case of ovarian stomal tumor with minor sex cord element.
Yeon Jung YOON ; Kyoung Young SUH ; Ji Yeon LEE ; Jong Yoo KIM ; Shin Ae LEE
Korean Journal of Obstetrics and Gynecology 1993;36(7):2756-2761
No abstract available.
3.A Case of Tick Infestation in Chest Wall by Ixodes Nipponensis.
Jong Phil CHU ; Yoo Joung CHO ; Gil Sang JEONG ; Byoung Mun KO
Korean Journal of Infectious Diseases 1997;29(1):53-56
We report herein an unusual case of tick bite. A 56 year old woman complained of erythematous nodular skin lesion on the left upper chest. An excision biopsy specimen of the lesion showed darkish-red nodule, in which there was a tick. It was identified as an adult female of Ixodes nipponensis by the scanning electron microscopic examination.
Adult
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Biopsy
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Female
;
Humans
;
Ixodes*
;
Middle Aged
;
Skin
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Thoracic Wall*
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Thorax*
;
Tick Bites
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Tick Infestations*
;
Ticks*
4.Clinical Study on Cesarean Hysterectomy.
Jong Dae WHANG ; Sang Yun OH ; Jin Kyoung YOO ; Soon Ha YANG ; Je Ho LEE ; Cheong Rae ROH
Korean Journal of Perinatology 2000;11(3):315-319
No abstract available.
Hysterectomy*
5.Scanning Electron Microscopic Observation on the Microvasculature in Anterior Segment of the Rabbit Eye Injected with Endothelin-1.
Eun Kyoung PARK ; Jong Heun LEE ; Ji Myong YOO
Journal of the Korean Ophthalmological Society 1997;38(8):1336-1341
Using Mercox CL-2B, intravascular casting was performed follwoing intracameral injection with 2.0microgram/microliter of Endothelin-1(ET-1) in the rabbit eye. One hour after intracameral injection with ET-1 caused severe focal andsegmental constriction of major arterial circle(MAC). Obstruction of the small branch of MAC caused localized filling defect and decreased number of capillaries in the vascular cast. The diameters of vascular cast were reduced at about 22.2% in proximal MAC, 22.4% in distal MAC and 21.8% in first branch of MAC. This results were suggested that ET-1 may play a role in the regulation of blood flow in the anterior segment vasculature of the rabbit.
Capillaries
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Constriction
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Endothelin-1*
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Microvessels*
6.The Influence of Hypothermia on Transfusion Requirement in Patients Who Received Clopidogrel in Proximity to Off-Pump Coronary Bypass Surgery.
Bo Ra LEE ; Jong Wook SONG ; Young Lan KWAK ; Kyoung Jong YOO ; Jae Kwang SHIM
Yonsei Medical Journal 2014;55(1):224-231
PURPOSE: Hypothermia adversely affects the coagulation that could be of clinical significance in patients receiving clopidogrel. We evaluated the influence of hypothermia on transfusion requirements in patients undergoing isolated off-pump coronary artery bypass surgery (OPCAB) who continued clopidogrel use within 5 days of surgery. MATERIALS AND METHODS: Protocol-based, prospectively entered data of 369 patients were retrospectively reviewed. The time-weighted average of intraoperative temperatures and the temperature upon ICU admission (TWA-temp) was assessed. Patients were divided into normothermia (> or =36degrees C, n=224) and hypothermia (<36degrees C, n=145) group. The transfusion requirement for perioperative blood loss was assessed and compared. RESULTS: Patients with hypothermia were older and had lower body surface area (BSA) than patients with normothermia. Age and BSA adjusted transfusion requirement was significantly larger in the hypothermia group [patients requiring transfusion: 64% versus 48%, p=0.003; number of units: 0 (0-2) units versus 2 (0-3) units, p=0.002]. In multivariate analysis of predictors of perioperative multiple transfusion requirements, hypothermia was identified as an independent risk factor along with age, female gender, BSA, chronic kidney disease, and congestive heart failure. CONCLUSION: Hypothermia was associated with increased transfusion requirement in patients undergoing OPCAB who received clopidogrel in proximity to surgery. Considering the high prevalence and the possibility of hypothermia being a modifiable risk factor, aggressive measures should be undertaken to maintain normothermia in those patients.
Aged
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Coronary Artery Bypass, Off-Pump/*methods
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Female
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Humans
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Hypothermia/*physiopathology
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Male
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Middle Aged
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Retrospective Studies
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Ticlopidine/*analogs & derivatives/therapeutic use
7.Predictive Performance of Glycated Hemoglobin for Incident Diabetes Compared with Glucose Tolerance Test According to Central Obesity
Suji YOO ; Jaehoon JUNG ; Hosu KIM ; Kyoung Young KIM ; Soo Kyoung KIM ; Jungwha JUNG ; Jong Ryeal HAHM ; Jong Ha BAEK
Endocrinology and Metabolism 2020;35(4):873-881
Background:
To examine whether glycated hemoglobin (HbA1c) test would be a suitable screening tool for detecting high-risk subjects for diabetes compared to oral glucose tolerance test (OGTT) according to accompanied central obesity.
Methods:
In this prospective population-based cohort study, both OGTT and HbA1c tests were performed and continued every 2 years up to 12 years among individuals with non-diabetic state at baseline (aged 40 to 69 years, n=7,512). Incident diabetes was established by a doctor, HbA1c ≥6.5%, and/or fasting plasma glucose (FPG) ≥126 mg/dL, and/or 2-hour postprandial glucose (2hPG) level based on OGTT ≥200 mg/dL. Discriminative capacities of high HbA1c (≥5.7%) versus high 2hPG (≥140 mg/dL) for predicting incident diabetes were compared using Cox-proportional hazard regression and C-index.
Results:
During the median 11.5 years of follow-up period, 1,341 (17.6%) developed diabetes corresponding to an incidence of 22.1 per 1,000 person-years. Isolated high 2hPG was associated with higher risk for incident diabetes (hazard ratio [HR], 4.29; 95% confidence interval [CI], 3.56 to 5.17) than isolated high HbA1c (HR, 2.79; 95% CI, 2.40 to 3.26; P<0.05). In addition, high 2hPG provided better discriminatory capacity than high HbA1c (C-index 0.79 vs. 0.75, P<0.05). Meanwhile, in subjects with central obesity, the HR (3.95 [95% CI, 3.01 to 5.18] vs. 2.82 [95% CI, 2.30 to 3.46]) and discriminatory capacity of incident diabetes (C-index 0.75 vs. 0.75) between two subgroups became comparable.
Conclusion
Even though the overall inferior predictive capacity of HbA1c test than OGTT, HbA1c test might plays a complementary role in identifying high risk for diabetes especially in subjects with central obesity with increased sensitivity.
8.Pattern of Cervical Neck Lymph Node Metastasis in Papillary Thyroid Carcinoma according to Tumor Size.
Gun KIM ; Hyun Jong KANG ; Kyoung Sik PARK ; Nam Sun PAIK ; Young Bum YOO
Korean Journal of Endocrine Surgery 2010;10(4):256-260
PURPOSE: Prognosis of papillary thyroid carcinoma (PTC) is very favorable, but PTC frequently invade adjacent soft tissue and metastases to cervical lymph node. We evaluated the pattern of cervical neck lymph node metastasis in PTC according to tumor size. METHODS: From August 2005 to January 2009, 353 patients were underwent surgery for PTC. Among these patients, total thyroidectomy with cervical neck lymph node dissection were done in 266 patients. We subdivided patients into four groups according to size and compared the clinicopathologic characters between groups. And we confirmed the factors affecting central neck node metastasis. RESULTS: The mean age of patients of diagnosis was 49.1 years and female to male ratio was 5.8:1. Cervical lymph node metastasis were in 47.0% of the total cases. Cervical lymph node metastases and invasion to adjacent structure increased with tumor size. But, there were no significant differences in tumor size, invasion to adjacent structure, multifocality or bilaterality according to cervical lymph node metastasis. Early diagnostic age and sexuality were significantly related to cervical lymph node metastasis of PTC. CONCLUSION: PTC showed the aggressiveness with increasing tumor size. Tumor size was not related to cervical lymph node metastasis. These findings suggest that tumor size can help treat PTC, can`t be used by prediction factor of cervical lymph node metastasis.
Diagnosis
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Female
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Humans
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Lymph Node Excision
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Lymph Nodes*
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Male
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Neck*
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Neoplasm Metastasis*
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Prognosis
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Sexuality
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Thyroid Gland*
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Thyroid Neoplasms*
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Thyroidectomy
9.The Anti-Inflammatory Effect of Arginine-Vasopressin on Lipopolysaccharide-Induced IkappaBalpha/Nuclear Factor-kappaB Cascade.
Jisoo PARK ; Eun Young EO ; Kyoung Hee LEE ; Jong Sun PARK ; Jae Ho LEE ; Chul Gyu YOO ; Choon Taek LEE ; Young Jae CHO
Korean Journal of Critical Care Medicine 2015;30(3):151-157
BACKGROUND: Arginine vasopressin (AVP) is widely used as a vasopressor agent. Some recent studies have suggested that AVP may exert an immunomodulatory effect. However, the mechanism about the anti-inflammatory effect of AVP is not well known. We investigated the effect of AVP on the ihibitor of kappa B (IkappaBalpha)/nuclear factor-kappa B (NF-kappaB) pathway in RAW 264.7 cells. METHODS: Cultured RAW 264.7 cells were pretreated with AVP and stimulated with lipopolysaccharide (LPS). To evaluate the effect of AVP on inflammatory cytokines, the concentration of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) were assessed by an enzyme-linked immunosorbent assay technique. The expression of IkappaBalpha and nuclear translocation of NF-kappaB p65 were measured by Western blotting, and IkappaB kinase (IKK) activity was analyzed by an in vitro immune complex kinase assay. To confirm the AVP effect on IkappaBalpha/NF-kappaB cascade and via V2 receptor, we added tolvaptan (V2 receptor antagonist) after AVP pretreatment. RESULTS: The increase of IL-6 and TNF-alpha in LPS-stimulated RAW 264.7 cells was suppressed by a treatment with AVP. Pretreatment of AVP inhibited increasing of IKK activity and IkappaBalpha degradation induced by LPS in RAW 264.7 cells. Furthermore, LPS induced and NF-kappaB transcription was inhibited by AVP pretreatment. The observed changes in IKK activity, IkappaBalpha degradation and NF-kappaB transcription by AVP was abolished by tolvaptan treatment. CONCLUSIONS: Our results suggest that AVP showed anti-inflammatory effect on LPS-induced IkappaBalpha/NF-kappaB cascade in mouse macrophages via V2 receptors.
Animals
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Antigen-Antibody Complex
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Arginine Vasopressin
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Blotting, Western
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Cytokines
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Enzyme-Linked Immunosorbent Assay
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I-kappa B Kinase
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Interleukin-6
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Macrophages
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Mice
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NF-kappa B
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Phosphotransferases
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Receptors, Vasopressin
;
Tumor Necrosis Factor-alpha
10.Factors Associated with Self-reported Fatigue: from Korean National Health and Nutrition Examination Survey 2001.
Jong Im SONG ; Eun Mi AHN ; Kyoung Woo KIM ; Hyun Kyung GWAK ; Min Seon PARK ; Sang Ho YOO
Journal of the Korean Academy of Family Medicine 2007;28(11):835-844
BACKGROUND: Fatigue is one of the common chief complaints in primary care setting. It causes disability in daily activity and lowers quality of life. This study was intended to investigate the information about fatigue useful in outpatient clinic. METHODS: The data was obtained from the Korean Health and Nutrition Survey 2001. Logistic regression analysis was performed to examine the association between self- reported fatigue and several factors including sociodemographic factors, life style factors, stress, depression, and worry about health. RESULTS: The prevalence of fatigue was low in unmarried status, more significantly in men when compared with those in married status. No significant association was found between fatigue and occupation, education level, monthly income, smoking, and alcohol consumption. The risk of fatigue in the exercising group more than 4 times per week was significantly low in women. The risk of fatigue in the group of sleeping duration less than 5 hours was significantly higher than the group of sleeping more than 9 hours. Compared with unemployed state, the daily working duration of more than 9 hours increased the risk for fatigue significantly. Stress perception, sadness or depression during the past 1 year and worry about health were strongly associated with fatigue. CONCLUSION: When evaluating and managing patients with fatigue, we should search for information about the patients' lifestyle such as exercise, daily work hours, sleeping as well as stress perception and depressive mood. Especially among the lifestyle, inquiring about regular exercise and promoting it may be important in order to manage patients with fatigue.
Alcohol Drinking
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Ambulatory Care Facilities
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Depression
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Education
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Fatigue*
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Female
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Humans
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Life Style
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Logistic Models
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Male
;
Nutrition Surveys*
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Occupations
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Prevalence
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Primary Health Care
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Quality of Life
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Single Person
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Smoke
;
Smoking