1.Effect of Stress on the Expression of Rho-Kinase and Collagen in Rat Bladder Tissue.
Hana YOON ; Donghyun LEE ; Kyemin CHUN ; Hyunsuk YOON ; Jaeyeong YOO
Korean Journal of Urology 2010;51(2):132-138
PURPOSE: We examined the effect of stress on the pathophysiology of bladder stability in terms of enzyme levels, Rho-kinase, and bladder relaxation. MATERIALS AND METHODS: A total of 48 female Sprague-Dawley rats were studied in scheduled stress environments for 7, 14, and 28 days; 24 rats were in the control group and 24 rats were in the test (stressed) group. RESULTS: Estrogen decreased significantly whereas testosterone and dopamine increased significantly in the stress group (p<0.05). Rho-kinase was significantly increased in the rats exposed to stress stimuli for 14 days (p<0.05). Collagen types I and III in the bladder tissue were significantly higher in rats exposed to stress for 14 days and 28 days (collagen type I in the 14-day group, p<0.01; collagen type I in the 28-day group, p<0.05; collagen type III in the 14-day and 28-day groups, p<0.05). Voiding frequency increased significantly as the duration of stress exposure was prolonged, in addition to a significant decrease in volume per voiding (p<0.05). CONCLUSIONS: The changes observed in micturition pattern, factors that contribute to smooth muscle contraction, and relaxation in the female rat bladder support the hypothesis that stress affects bladder stability.
Animals
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Collagen
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Collagen Type I
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Collagen Type III
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Contracts
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Dopamine
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Estrogens
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Female
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Humans
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Muscle, Smooth
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Rats
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Rats, Sprague-Dawley
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Relaxation
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rho-Associated Kinases
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Testosterone
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Urinary Bladder
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Urination