Epithelioid hemangioendothelioma is a recently described vascular neoplasm characterized by epitheloiod endothelial linings and its borderline biologic behavior. We report a case of epithelioid hemangioendothelioma in a 6-year-old male. The tumor was presented as a non-tender dermal nodule on the left middle finger. The microscopic sections revealed irregular proliferation of vascular channels lined by epithelioid cells with histiocytoid features and frequent intracytoplasmic vacuoles, mimicking so called signet ring cells. Immunohistochemical stainings for factor VIII and vimentin were positive both in epithelioid and vacuolated endothelial cell. Electron microscopic finding reveals abundant intermediate filaments.

Cutaneous chromomycosis is a slow growing verrucous skin infection caused by five species, identified as Philadophara verruscosa, Fonsecaea dermatitidis, Fonsecaea pedrosoi, Fonsecaea compactum, Cladosporium, carionii. We report a case of chromomycosis of the skin causing by Cladosporium genus, in view of its rarity and unique light microscopic and ultrastructural features. This 65-year-old female had suffered from eczematous and lichenified skin lesion for the last two years since she burned her dorsum of the left hand by boiling oil. The lesion was slowly progressed, and ended up to the brownish hardening of the skin covered with crusts. Skin punch biopsy was done. Histologically, the sections revealed typical features of chronic granulomatous inflammation along with the heavy infiltration of the lymphocytes, plasma cells and histiocytes. Pseudocarcinomatous downgrowth of the epidermis and microabscesses in the upper dermis were also found. Numerous thick walled fungal organisms were noted within the giant cells and freely in the dermis, which were positive ant PAS stain, and diastase resistant. Thick mucous capsule is not observed. The organisms showed very faint yellowish walls with a few buddings and a few short elongated hyphae were also noted. Ultrastructurally, the fungus wall was multilayered and inner organelles were sparse. There is no evidence of systemic involvement, especially brain.
Female ; Humans ; Biopsy

Three cases of granular cell tumor in esophagus removed endoscopically are present. Immunohistochemical and electron microscopic studies confirmed that this tumor is originated from schwann cell.

In order to elucidate the possibility of diagnosis in soft tissue cysticercosis in case no parasite in it, immunohistochemical staining with antihuman IgG, IgM, and IgA and anticysticercus antibody in tissue section were carried out. Not only the differential counts of infiltrated plasma cells but also the parasite itself against to the anticysticercus antibody were observed. Materials stained immunohistochemically were consisted of 21 soft tissue cysticercosis with or without parasite. The results were as follows: 1) IgG producing plasma cells were most frequent (45.8+/-18.7) and followed by IgM (14.3+/-0.43) and (4.3+/-5.71). Number of IgG plasma cells were more frequently accompanied in the presence of cysticercus (55.9+/-17.7) than in the absence of parasite (39.7+/-17.1). 2) The mean+/-S.D. of plasma cells positive to the anticysticercus antibody were 28.2+/-18.7. And anticysticercus antibody producing plasma cells were more frequent in the presence of cysticercus (49.2+/-5.16) than in the absence of parasite (15.2+/-9.46). 3) Antigenicity of the cysticercus was strong in inner layer(#) followed by tegument(+) and fibrous layer(-). 4) Therefore, it could be suggested that antibody against to the cysticercus is mainly produced by IgG plasma cells and antigenicity of the worm is strong in the inner layer of cyst wall. In addition, there is a possibility of assisting the diagnosis of cysticercosis with anticysticercus antibody by immunohistochemically.
Cysts

The cutaneous lymphadenoma is a recently described tumor with a distinctive histologic picture representing a basaloid epithelial proliferation and intraepithelial lymphocytic infiltration; it seems to be a benign adnexal neoplasm of uncertain histogenesis. We documented one example of cutaneous lymphadenoma showing typical histologic features. The tumor typically presented as a well circumscribed nodule with scant or no epidermal connections. The proliferating one consisted of multiple rounded lobules of basaloid cells with some degree of peripheral palisading. There was an intense infiltrate of small lymphocytes within the lobules but few in the stroma. No clear adnexal differentiation is noted. Immunohistochemically, the basaloid cells show weak immunoreactivity for high molecular weight keratin and carcinoembryonic antigen, small lymphocytes for T-cell marker and some dendritic cells for S-100 protein. After surgical resection, we found no evidence of local recurrence or distant metastasis for four years, so we considered this tumor as a benign one and diagnosed as cutaneous lymphadenoma by typical histologic features.
Carcinoembryonic Antigen ; Dendritic Cells ; Lymphocytes ; Molecular Weight ; Neoplasm Metastasis ; Recurrence ; S100 Proteins ; Skin ; T-Lymphocytes

No abstract available.
Cell Line* ; Hantaan virus* ; Humans*

No abstract available.
Administration, Oral* ; Animals ; Intestine, Large* ; Rats*

No abstract available.
Administration, Oral* ; Animals ; Intestine, Large* ; Rats*

The bacteria as well as the fungi can build up resistance against different kinds of drugs or can decrease in sensitivity to these drugs. Therefore it is necessary that we use the MIC test for acertain period of time to determine the right concentration of drug to use in therapy. The culturing of P. orbiculare was successful in 1951. Up until that time, there were few drugs available to use in the MIC test. So, treatment for Tinea versicolor was dependent upon ones previous experience in determining the drug's effective concentration. Therefore, we performed the MIC test of clotrimazole, sodium thiosulfate, selenium sulfide, propylene glycol against P. orbiculare. The diagnos is for Tinea versicolor in the patient was confirmed by using KOH smear and by the clinical manifeststion. The fungi were then isolated in a culture from an olive oil rich media Mixture of isolated fungus and olive oil was applied to the lateral side of a healthy male's right upper extremity. After one week, P. orbiculare was confirmed by wood lamp examination, KOH smear and culture. After confirmation of P. orbiculare, a variety of concentrated drugs were used. Then media, containing propylene glycol, sodium thiosulfate, selenium sulfide and clotrimazole with a small amount of olive oil was used in inoculations of 10 viable unit/ml of P.orbiculare in attempt to estblish the MIC test. Test results are as follows: l) Propylene glycol MIC Range; 12. 5mg/ml~7. 5mg/ml. 2) Sodium thiosulfate MIC Range; 500mg/ml~7. 5mg/ml. 3) Selenium sulfide MIC Range; l. 25mg/ml~0. 04mg/ml. 4) Clotrimazole MIC Range; 0. 5mg/ml~ 0. 0005mg/ml.
Bacteria ; Clotrimazole ; Fungi ; Humans ; Malassezia* ; Olea ; Propylene Glycol ; Selenium ; Sodium ; Tinea Versicolor ; Upper Extremity ; Wood ; Olive Oil

No abstract available.
Animals ; Liver* ; Rats* ; Selenium*