1.Expression, Characterization and Chain Shuffling of an Anti-HBsAg Phage Antibody.
In Hak CHOI ; Ik Jung KIM ; Suk Jin CHOI ; Hyo Jung KIM ; Jun Ho CHUNG ; Kye Sook YI ; Sang Do LEE ; Pann Ghill SUH
Journal of the Korean Society for Microbiology 1998;33(3):307-316
In our previous report an anti-HBsAg human monoclonal antibody was generated using antibody phage display library technique. Using pComb3 filamentous phagemid vector, Fab molecule was expressed in fusion form to a phage coat protein in the periplasm of E. coli. A clone of HBsAg binder was selected after panning and designated as B7. In order to select the clones with higher affinity and to examine which chain contributes most to the affinity of B7, the light and heavy chain of B7 was sequentially deleted and replaced with new library. HBsAg-binders were selected and tested by EIA (enzyme immunoassay). It was revealed that the affinity of B7 depends only on the heavy chain of Fd. B7 Fd was constructed without light chain and specificity and affinity was further confirmed by western blot analysis. This human monoclonal Fd antibody was found to react with d antigenic determinant of HBsAg as the original clone did. The nucleotide sequence analysis revealed that VH of B7 could be classified into Kabat's subgroup II and human IgG heavy chain family IV. The CH1 of B7 was IgG1.
Bacteriophages*
;
Base Sequence
;
Blotting, Western
;
Clone Cells
;
Hepatitis B Surface Antigens
;
Humans
;
Immunoglobulin G
;
Periplasm
;
Sensitivity and Specificity
2.Mass Production and Characterization of Anti-HBsAg Human Antibody B7 Fd.
In Hak CHOI ; Ik Jung KIM ; Jun Ho CHUNG ; Suk Jin CHOI ; Jong Bum IM ; Kye Sook YI ; Pann Ghill SUH ; Sung Ho RYU
Journal of the Korean Society for Microbiology 1999;34(3):265-275
We expressed anti-HBsAg human antibody fragment (B7 Fd) using pRSET-A vector and BL21(DE3)pLysS strain of E. coli. B7 Fd is composed only of variable domain (VH) and CH1 constant domain of IgG heavy chain molecule. This Fd molecule was solubilized using guanidine salt and then expressed in the form of inclusion body and successfully refolded into functional antibody molecule by rapid dilution in refolding buffer. B7 Fd reacted with d epitope of hepatitis B virus surface antigen (HBsAg). Its affinity was determined by competition enzyme-linked immunosorbent assay (competition ELISA). The K value of B7 Fd was 3.3 * 10.
Antigens, Surface
;
Enzyme-Linked Immunosorbent Assay
;
Guanidine
;
Hepatitis B virus
;
Humans*
;
Immunoglobulin G
;
Inclusion Bodies
3.A Case of Reflex Sympathetic Dystrophy Syndrome developed after Protein Losing Enteropathy.
Kyoung Ju YI ; Pum Soo KIM ; Won PARK ; Jeong Soo SONG ; Jae Nam CHANG ; Won CHOI ; Don Haeng LEE ; Hyung Gil KIM ; Kye Sook KWON ; Hueon Geun CHO ; Yong Woon SHIN ; Young Soo KIM ; In Young HYUN
Korean Journal of Medicine 1999;56(1):98-102
A 16-year old girl was admitted for chronic diarrhea, generalized edema, severe pain of lower extremities, and general weakness. She could not walk because of burning pain of knee and ankle. 99mTc-MDP bone scan demonstrated increased periarticular uptake in both lower extremity with focal increased activity in proximal portion of both tibias.. Abnormal abdominal activity in ascending, transverse, and descending colon is also seen. Bone densitometry showed severe osteoporosis in lower extremity. 99mTc-HSA scan showed abnormal radioactivity in small bowel, and descending colon indicating protein losing enteropathy. Endoscopic biopsy of terminal ileum showed a few dilated lymphatics in the submucosa. After steroid and supplemental therapy, the symptoms and signs of both protein losing enteropathy and RSDS much improved simultaneously. Reflex sympathetic dystrophy syndrome is a complex of symptoms characterized by severe pain, swelling, autonomic vasomotor dysfunction, and impaired mobility of affected extremities. The important causes are trauma, fracture, and inflammation. We assumed that the reflex sympathetic dystrophy syndrome of this girl was due to protein losing enteropathy. And as far as we know there has not been reported case that protein losing enteropathy assumed as a cause of reflex sympathetic dystrophy syndrome.
Adolescent
;
Ankle
;
Biopsy
;
Burns
;
Colon, Descending
;
Densitometry
;
Diarrhea
;
Edema
;
Extremities
;
Female
;
Humans
;
Ileum
;
Inflammation
;
Knee
;
Lower Extremity
;
Osteoporosis
;
Protein-Losing Enteropathies*
;
Radioactivity
;
Reflex Sympathetic Dystrophy*
;
Reflex*
;
Technetium Tc 99m Aggregated Albumin
;
Technetium Tc 99m Medronate
;
Tibia
4.Effects of Low Dose versus High Dose Statin Therapy on the Changes of Endothelial Function and Carotid Intima-Media Thickness in Patients with Variant Angina.
Kye Hun KIM ; Sook Hee CHO ; Yi Rang YIM ; Kyung Jin LEE ; Ju Hyup YUM ; Hyun Ju YOON ; Nam Sik YOON ; Young Joon HONG ; Hyung Wook PARK ; Ju Han KIM ; Youngkeun AHN ; Myung Ho JEONG ; Jeong Gwan CHO ; Jong Chun PARK
Journal of Cardiovascular Ultrasound 2013;21(2):58-63
BACKGROUND: To compare the effects of low dose and high dose of statin treatment on endothelial function and carotid intima-media thickness (IMT) in patients with variant angina (VAP). METHODS: A total of 70 patients with VAP were divided into two groups; atorvastatin 10 mg treatment group (group I: n = 35, 54.2 +/- 12.5 years) versus atorvastatin 40 mg treatment group (group II: n = 35, 52.6 +/- 9.8 years). Flow mediated vasodilation (FMD) of the brachial artery and IMT of the carotid artery were compared between the groups after 6 months of statin treatment. RESULTS: The baseline FMD and carotid IMT were not different between the groups. After 6 months of statin therapy, FMD was significantly improved in both groups (7.7 +/- 2.5% to 8.9 +/- 2.2% in group I, p = 0.001, 7.9 +/- 2.7% to 9.5 +/- 2.8% in group II, p < 0.001), but the degree of FMD change and FMD at 6 month were not different between the groups. Carotid IMT were not changed in both groups after 6 months of statin therapy. CONCLUSION: The use of statin for 6 months significantly improved endothelial function in patients with VAP, but carotid IMT was not changed. The use of high dose statin did not show significant additional benefit as compared with the use of low dose statin. The present study suggested that statin therapy would be beneficial in the treatment of VAP.
Atorvastatin Calcium
;
Brachial Artery
;
Carotid Arteries
;
Carotid Intima-Media Thickness
;
Heptanoic Acids
;
Humans
;
Hydroxymethylglutaryl-CoA Reductase Inhibitors
;
Pyrroles
;
Vasodilation
5.A Case of Gastric Glomus Tumor with Bleeing.
Hyun Joo SHIN ; Kyung Soo PARK ; Jae Su YI ; Kye Sook KWON ; Hyeon Geun CHO ; Won CHOI ; Don Haeng LEE ; Pum Soo KIM ; Hyong Kil KIM ; Yong Woon SHIN ; Young Soo KIM ; Jun Mi KIM
Korean Journal of Gastrointestinal Endoscopy 2000;20(2):123-126
A glomus tumor is a benign neoplasm arising from the neuromyoarterial glomus body, a specialized arteriovenous communication. It functions as a temperature regulator, predominantly in the skin. The glomus tumor occurs rarely in the stomach. The common presenting symptoms are upper gastrointestinal bleeding, epigastric discomfort, nausea, and vomiting. This benign tumor is seen as an intramural mass and is most frequently located in the gastric antrum. Because of its highly cellular nature, the frozen section is often misinterpreted as malignant, and an unduly extensive resection is sometimes performed. A 57-year-old woman was recently admitted due to hematemesis and melena for one day. A gastrofibroscopy was performed and a 3 4 cm sized submucosal mass with central ulceration was found on the anterior wall of the antrum. Fresh blood clots were noted on the ulcer base. Due to recurrent bleeding, a hemigastrectomy was performed. Pathologic findings, including immunoreactivity to alpha-smooth muscle actin and vimentin, was consistent with a glomus tumor of the stomach.
Actins
;
Female
;
Frozen Sections
;
Glomus Tumor*
;
Hematemesis
;
Hemorrhage
;
Humans
;
Melena
;
Middle Aged
;
Nausea
;
Pyloric Antrum
;
Skin
;
Stomach
;
Ulcer
;
Vimentin
;
Vomiting
6.Impact of Age on Clinical Outcomes in Middle-aged Korean Female Patients with Acute Myocardial Infarction - Based on a Cut-off Age of 55 Years.
Mi Sook OH ; Myung Ho JEONG ; Seung Hun LEE ; Jung Ae RHEE ; Jin Su CHOI ; In Hyae PARK ; Chung KIM ; Eun Jung KIM ; Hyun Yi KOOK ; Ki Hong LEE ; Doo Sun SIM ; Kye Hun KIM ; Young Joon HONG ; Hyung Wook PARK ; Ju Han KIM ; Young Keun AHN ; Jeong Gwan CHO ; Jong Chun PARK ; Sang Hyung KIM
Korean Journal of Medicine 2016;91(2):158-165
BACKGROUND/AIMS: It is well known that the menopause is related to interference in lipid metabolism, obesity, and a hypercoagulable state. The aim of the present study was to examine the impact of the menopause in middle-aged Korean females with acute myocardial infarction (AMI). METHODS: A total of 1,781 middle-aged females (aged < 65 years) in the Korean Acute Myocardial Infarction registry were enrolled into this study between November 2005 and December 2013. The patients were divided into two groups; the pre-menopause group (≤ 55 years old) and the menopause group (56-64 years old). Major adverse cardiac events (MACE) were analyzed over a one-year follow-up period. RESULTS: The pre-menopause and menopause groups comprised 669 patients (mean age, 49.1 ± 5.6 years) and 1,112 patients (mean age, 60.6 ± 2.6 years), respectively. The incidence of hypertension (42.2% vs. 59.4%, p < 0.001), diabetes mellitus (DM) (27.4% vs. 35.7%, p < 0.001), and dyslipidemia (12.9% vs. 17.7%, p = 0.008) were more frequent in menopausal patients. Additionally, the rates of smoking (20% vs. 12.7%, p < 0.001) and familial history (12% vs. 6.8%, p < 0.001) were higher in the pre-menopause group. The cumulative rates of MACE did not show any differences between the two groups. A history of atrial fibrillation, previous AMI and DM, higher Killip class, and multi-vessel disease were independent risk factors for predicting one-year MACE. CONCLUSIONS: The survival analysis demonstrated that there was no significant difference in MACE rates between the pre-menopause and menopause groups during the one-year follow-up. Therefore, middle-aged pre-menopausal women should be treated more intensively, regardless of whether they are menopausal.
Atrial Fibrillation
;
Diabetes Mellitus
;
Dyslipidemias
;
Female*
;
Follow-Up Studies
;
Humans
;
Hypertension
;
Incidence
;
Lipid Metabolism
;
Menopause
;
Myocardial Infarction*
;
Obesity
;
Premenopause
;
Prognosis
;
Risk Factors
;
Smoke
;
Smoking