House dust mites, Dermatophagoides farinae and Dermatophagoides pteronyssius have been identified as the main sources of house dust allergen and are considersed the most important causative allergen in the ethology of allergic respiaratory disease and allergic rhinitis. To evaluate the incidence of positive skin reactivity to house dust mites and relationship between total serum IgE levels and specific IgE antibody to house dust mites, we performed allergic skin test (scratch) in 182 children with allergic respiatory disease and atopic dermatitis. We also checked serum total IgE by PRIST method and specific IgE antibody to D. farinae and D. pteronyssinus by ELISA. Our results showed that positive skin reaction was observed in 79% of patients in D. farinae, 71% in D. pternyssinus. There was a significant correlation between serum total IgE and IgE antibody specific to D. farinae (r=0.55, p<0.001). Serum total IgE and specific IgE antibody to D. petronyssinus had no significant correlation (r=0.39, p<0.001). D. farinae and D. pteronyssinus had close correlation in the skin test (r=0.82, p<0.0001) and relative concentration of specific IgE antibody (r=0.73, p<0.0001). The agreement between skin reaction and relative concentration of specific IgE to House dust mites was 68% in D. farinae and 63% in D.pteronyssinus.
Child* ; Dermatitis, Atopic ; Dermatophagoides farinae ; Dust* ; Enzyme-Linked Immunosorbent Assay* ; Ethology ; Humans ; Immunoglobulin E* ; Incidence ; Pyroglyphidae* ; Rhinitis ; Skin Tests ; Skin*