Apocrine hidrocystoma is an uncommon benign tumor derived from the secretory segment of apocrine sweat glands and another name for it is apocrine cystadenoma. A 48-year-old female patient had a solitary, translucent red-brownish cystic nodule, 10 × 6 × 7mm in size, on the periorbital area of her left eye. The lesion had enlarged very slowly for 25 years. We excised the lesion and identified it as apocrine hidrocystoma. This is a case of apocrine hidrocystoma with typical clinical and histopathological findings.
Cystadenoma ; Female ; Hidrocystoma* ; Humans ; Middle Aged ; Sweat Glands

BACKGROUND: Laser resurfacing has beneficial effects for the treatment of several skin conditions. Recently, the pulsed erbium:YAG laser has been shown to be a highly effective treatment for several kinds of pitted facial scars. OBJECTIVE: The purpose of this study was to assess the efficacy and safety of pulsed erbium:YAG laser skin resurfacing at the setting of low or high energy for pitted acne scars. METHODS: 65 patients with pitted acne scars were included in this study. All patients had skin types III or IV. All patients were instructed to use tretinoin cream 0.05% nightly for 2-4 weeks prior to the laser treatment. The pulsed erbium:YAG laser with a 2 mm handpiece at the setting of 500 mJ/pulse, 3.5-5 W in 45 patients and 1,000 mJ/pulse, 7-10 W in 20 patients was used. Two weeks after laser treatment, topical application of hydroquinone 4%, tretinoin 0.05%, and hydrocortisone 1% cream was recommended for 2-4 weeks. Facial photographs were obtained at baseline and 2 week intervals postoperatively. The results of treatment were evaluated for the changes of skin texture and color at 1 month, 3 months, and 6 months. RESULTS: In 45 cases treated with 500 mJ/pulse energy, pitted acne scars were improved about 46.7 % on average and no erythema and postinflammatory hyperpigmentation was observed after 6 months. In 20 cases treated with 1,000 mJ/pulse energy, pitted acne scars were improved about 64% on average and erythema in two patients and postinflammatory hyperpigmentation in one patient were observed after 6 months. CONCLUSION: The results of laser treatment for pitted facial acne scars at the setting of high energy are better than those of laser treatment at the setting of low energy.
Acne Vulgaris* ; Cicatrix* ; Erbium* ; Erythema ; Humans ; Hydrocortisone ; Hyperpigmentation ; Lasers, Solid-State* ; Skin ; Tretinoin

No abstract available.
Adenocarcinoma*

No abstract available.
Mongolian Spot* ; Nevus, Blue*

No abstract available.
Breast* ; Lichen Sclerosus et Atrophicus* ; Lichens* ; Vitiligo* ; Vulva*

No abstract available.
Adult* ; Humans ; Myofibroma* ; Thigh*

No abstract available.
Adult* ; Humans ; Myofibroma* ; Thigh*

BACKGROUND: Multidrug-resistant (MDR) Acinetobacter spp. acquire antimicrobial agent-resistance genes via class 1 integrons. In this study, integrons were characterized to investigate the antimicrobial resistance mechanisms of MDR Acinetobacter isolates. In addition, the relationship between the integron type and integron-harboring bacterial species was analyzed by using epidemiological typing methods. METHODS: Fifty-six MDR Acinetobacter spp.-A. baumannii (N=30), A. bereziniae (N=4), A. nosocomialis (N=5), and A. pittii (N=17)-were isolated. The minimum inhibitory concentrations (MICs) were determined on the basis of the results of the Epsilometer test (Etest). PCR and DNA sequencing was performed to characterize the gene cassette arrays of class 1 integrons. Multilocus sequence typing (MLST) and repetitive extragenic palindromic sequence (REP)-PCR were performed for epidemiological typing. RESULTS: Class 1 integrons were detected in 50 (89.3%) of the 56 isolates, but no class 2 or 3 integron was found within the cohorts. The class 1 integrons were classified into 4 types: 2.3-kb type A (aacA4-catB8-aadA1), 3.0-kb type B (aacA4-blaI(MP-1)-bla(OXA-2)), 3.0-kb type C (bla(VIM-2)-aacA7-aadA1), and 1.8-kb type D (aac3-1-bla(OXA-2)-orfD). Type A was most prevalent and was detected only in A. baumannii isolates, except for one A. bereziniae isolate; however, type B was amplified in all Acinetobacter isolates except for A. baumannii isolates, regardless of clone and separation time of the bacteria. CONCLUSIONS: Although class 1 integron can be transferred horizontally between unrelated isolates belonging to different species, certain types of class 1 integrons tend to transfer horizontally and vertically among A. baumannii or non-baumannii Acinetobacter isolates.
Acinetobacter/drug effects/isolation & purification/*metabolism ; Acinetobacter Infections/epidemiology/microbiology ; Acinetobacter baumannii/drug effects/isolation & purification/metabolism ; Anti-Bacterial Agents/pharmacology ; DNA, Bacterial/chemistry/metabolism ; Drug Resistance, Multiple, Bacterial ; Humans ; Integrons/*genetics ; Microbial Sensitivity Tests ; Multilocus Sequence Typing ; Polymerase Chain Reaction ; Republic of Korea

BACKGROUND: Pseudomonas aeruginosa is a clinically important pathogen that causes opportunistic infections and nosocomial outbreaks. Recently, the type III secretion system (TTSS) has been shown to play an important role in the virulence of P. aeruginosa. ExoU, in particular, has the greatest impact on disease severity. We examined the relationship among the TTSS effector genotype (exoS and exoU), fluoroquinolone resistance, and target site mutations in 66 carbapenem-resistant P. aeruginosa strains. METHODS: Sixty-six carbapenem-resistant P. aeruginosa strains were collected from patients in a university hospital in Daejeon, Korea, from January 2008 to May 2012. Minimum inhibitory concentrations (MICs) of fluoroquinolones (ciprofloxacin and levofloxacin) were determined by using the agar dilution method. We used PCR and sequencing to determine the TTSS effector genotype and quinolone resistance-determining regions (QRDRs) of the respective target genes gyrA, gyrB, parC, and parE. RESULTS: A higher proportion of exoU+ strains were fluoroquinolone-resistant than exoS+ strains (93.2%, 41/44 vs. 45.0%, 9/20; P< or =0.0001). Additionally, exoU+ strains were more likely to carry combined mutations than exoS+ strains (97.6%, 40/41 vs. 70%, 7/10; P=0.021), and MIC increased as the number of active mutations increased. CONCLUSIONS: The recent overuse of fluoroquinolone has led to both increased resistance and enhanced virulence of carbapenem-resistant P. aeruginosa. These data indicate a specific relationship among exoU genotype, fluoroquinolone resistance, and resistance-conferring mutations.
ADP Ribose Transferases/genetics ; Anti-Bacterial Agents/*pharmacology ; Bacterial Proteins/genetics ; Bacterial Toxins/genetics ; Carbapenems/pharmacology ; Drug Resistance, Bacterial/*drug effects ; Fluoroquinolones/*pharmacology ; Genotype ; Humans ; Microbial Sensitivity Tests ; Multilocus Sequence Typing ; Mutation ; Pseudomonas aeruginosa/*genetics/isolation & purification/pathogenicity ; Sputum/microbiology ; Virulence

BACKGROUND: Keratoacanthoma(KA) is common, benign cutaneous tumor that is most likely derived from hair follicle cells, and most often occurs on sun-exposed sites in light-skinned persons of middle age or older. Some authors believed that keratoacanthoma is a variant of squamous cell carcinoma(SCC), which it often resembles clinically and histopathologically. So, the distinction between KA and SCC is still debatable and a matter of speculation. OBJECTIVE: The purpose of this study was to investigate the clinicohistopathologic findings of KA and its expression patterns, compared with SCC, of immunohistochemical staining using Ki-67, p53, bcl-2, and involucrin. METHODS: This clinicohistopathologic study included 20 cases that had confirmed to KA. We performed the immunohistochemical staining with Ki-67, p53, bcl-2 in 14 and with involucrin in 13 KAs. Ki-67, p53, bcl-2, and involucrin staining was also performed in 6 SCCs. RESULTS: All of KAs were solitary lesions. The male to female ratio was 1.2:1. The average age was 63.315.1 years and 11 of 20(55.5%) developed in sixth and seventh decades. The site of predilection were sun-exposed areas(85%) such as face, forearm, neck. Histologically, all of KAs were fully-developed lesions, and 17 of 20(85%) were dome or berry-shaped type II KAs. 17 of 20(85%) KAs showed exo-endophytic growth pattern. Common histologic findings in this study were horn-filled crater formation(80%), epidermal collarette(85%), epidermal proliferation in the perilesional area and the base of crater(100%), lateral extension tendency(80%), individual keratinization(75%), intraepithelial neutrophilic microabscess(65%), and eosinophilic glassy appearance in kertinocytic cytoplasm(95%). In immunohistochemical study using Ki-67, p53, bcl-2, and involucrin, there were no significant differences between KA and SCC except of the distribution pattern of Ki-67 positive cells in tumor. Ten of 14(71.4%) KAs revealed the marginal pattern, whereas no SCCs showed marginal pattern in Ki-67 staining. CONCLUSION: KA is a rapidly growing, cutaneous tumor that most often occurs on sun-exposed sites in sixth and seventh decades. Our results showed that KA is almost similar to SCC immunohistochemically, we suggest that KA is a type of SCC. And also, the characteristic distribution pattern of Ki-67 positve cells is helpful in making differentiation between KAs and SCCs.
Eosinophils ; Female ; Forearm ; Hair Follicle ; Humans ; Keratoacanthoma* ; Male ; Middle Aged ; Neck ; Neutrophils