1.The Significance of Expression of Matrix Metalloproteinases (MMPs) in Intimal Hyperplasia after Ballon Injured Rat Aorta.
Kwaung Su KIM ; Woo Hung KWUN ; Bum Seok KIM ; Bo Yang SUH ; Koing Bo KWUN ; Suk Hwan BAEK
Journal of the Korean Society for Vascular Surgery 2002;18(1):7-14
PURPOSE: Vascular smooth muscle cells (VSMCs) migration and proliferation are important for neointimal formation after arterial injury. Migration of VSMCs requires degradation of basement membrane and extracelluar matrix surrounding the cell, and there is increasing evidence that VSMCs produce extracelluar matrix-degradating proteinases, such as matrix metalloproteinases (MMPs) after arterial injury. To assess the role of MMPs in VSMCs proliferation, migration and intimal thickening, we measured the expression of MMP-2 and MMP-9 in the balloon-injured rat aorta model. METHOD: Twenty-five male Sprague-Dawley rats weighting of 250~300 gm were underwent aortic intimal denudation with 2F balloon catheter. Aorta was harvested at various time intervals of 1, 3, 5, 7, 21 days and then analyzed the MMP expression used by gelatin zymography. Intimal hyperplasia caused by balloon injury was confirmed by microscopic examination. RESULT: MMP-2 (72 kD) was constitutively expressed in the normal aorta and was not increased substantially after injury. But the expression of 62 kd forms, which is activated form of MMP-2, was significantly increased during the period of 5 through 7 days after injury (P<0.05). The expression of MMP-9 (92 kD) was significantly increased at 1st day after injury and diminished thereafter (P<0.05). CONCLUSION: These results suggest that activated MMP-2 (62 kD) and MMP-9 (92 kD) may play an important role in VSMCs migration and formation of intimal hyperplasia after arterial injury. And the activated form of MMP-2 (62 kD) seems to be involved mainly in degradation of basement membrane and matrix.
Animals
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Aorta*
;
Basement Membrane
;
Catheters
;
Gelatin
;
Humans
;
Hyperplasia*
;
Male
;
Matrix Metalloproteinases*
;
Muscle, Smooth, Vascular
;
Peptide Hydrolases
;
Rats*
;
Rats, Sprague-Dawley
2.Arm Span-Height Relationship for Prediction of Spirometric Values in Korean Adult Women.
Won Jung KOH ; Young Su JU ; Tae Yub KIM ; Jae Sung PARK ; Seung Do YU ; Kwaung Soo CHOI ; Domyung PAEK ; Sung Koo HAN ; Young Soo SHIM
Tuberculosis and Respiratory Diseases 1999;46(6):786-794
BACKGROUND: Arm span measurements provide a practical substitute for standing height to predict normal spirometric values in subjects unable to stand or those with a skeletal deformity such as kyphoscoliosis. The relationship between arm span and height has previously been reported as either a fixed ratio unaffected by age or as a regression equation in which the ratio varies as a function of age. The fixed ratio or regression equation is known to be specific for sex and race. METHODS: We studied the relationship between standing height, arm span, and age in 381 Korean adult female subjects (ages 20 to 69 yrs) sampled in a general population. RESULTS: The mean ratio for arm span to height is 1.004. Multiple linear analysis found arm span and age to be predictive of standing height (p=0.0001, r2=0.76). We performed the analysis of the difference between the predicted height using either fixed ratio or regression equation and actual height. At the extremes of arm span and age, the ratio method either underestimated (at smaller arm span or younger age) or overestimated (at larger arm span or older age) as compared with actual height (p=0.0001). CONCLUSION: This results indicate that the estimated height using the fixed ratio method provides a less acceptable method of estimating height for the prediction of lung volumes in the Korean adult women when compared with the regression equations, especially at the extremes of stature or age.
Adult*
;
Arm*
;
Congenital Abnormalities
;
Continental Population Groups
;
Female
;
Humans
;
Lung
;
Spirometry