No abstract available.
Hyperplasia* ; Leishmania donovani* ; Leishmania*

No abstract available.
Hyperplasia* ; Leishmania donovani* ; Leishmania*

In the present study we conducted a regression analysis of age at menarche against Maximum Peak Velocity (MPV) of height in non-athlete Korean girls which was derived with the wavelet interpolation method, and composed linear to quartic regression polynomials to obtain the best regression polynomial. We then applied the age at menarche and age at MPV of height of athletes to the best polynomial regression evaluation, and investigated the validity of a delayed menarche evaluation that we constructed. Moreover, the relation between delayed menarche and menstrual status was examined by investigating delayed menarche and menstrual pain in individuals for the first time. The subjects were 124 second year female students at a physical education high school in the suburbs of Pusan, South Korea. A questionnaire survey of these girls was conducted, from which their date of birth, age at menarche, and athletic activities in elementary, junior high, and high school were obtained. In addition, health check records were examined retrospectively, and longitudinal growth data for height were obtained from the 1st year of elementary school (7 years old) until the second year of high school (17 years old). Next, the same survey as above was also done for second year students at a general high school in the same area, as a control group. Three hundred forty-five non-athletes for whom all data were available were selected. As the results, the third order polynomial was found to be most suitable for the regression polynomial. When it was applied to individual female Korean athletes with respect to the regression evaluation, positive scores were obtained for many athletes and an overall delay in menarche was seen. Delayed menarche was not seen, however, in archery athletes. A strong delay in menarche was thus found in Korean athletes. And it was shown that 80% had moderate or greater menstrual pain and a close relation with menstrual abnormalities, the effectiveness of the delayed menarche evaluation was further validated.

No abstract available.
Testis*

PURPOSE: We report a case of chronic myelogenous leukemia(CML) involving the retina and optic nerve, in which visual acuity had been improved after radiation therapy combined with steroid pulse therapy. METHODS: In a 44-year-old CML patient with central nervous system involvement who presented with retinal infiltration, initial uncorrected visual acuity was 1.0 in the right eye, 0.9 in the left eye. The patient was given 1% prednisolone acetate eye drops 4 times a day and 40mg of oral prednisolone a day. One month later, papilledema and exudative retinal detachment was developed in the left eye and we raised oral prednisolone dosage to 90mg a day. In spite of those kinds of treatment, papilledema in left eye and retinal infiltration in both eyes were much more aggravated at two months. So we used high-dose steroid(solumedrol 125mg qid) parenterally for initial 3 days followed by tapered oral dosage(prednisolone 60mg/day for 11 days, tapered with 10mg/day and maintain with 10mg/day) and performed simultaneously radiation therapy (total 2340 rads, 13 times) on both eyes through 2 weeks. RESULTS: Uncorrected visual acuity was decreased to 0.3 in the left eye at one month and to 0.6 in the right eye and 0.08 in the left eye at two months, and improved to 1.0 in the right eye and 0.5 in the left eye. Gradual resolution was noted in retinal and optic disc abnormalities at one month after high-dose steroid therapy combined with radiation therapy. CONCLUSIONS: This case suggests that visual acuity in CML patient with the retinal and optic nerve involvement can be improved by using radiation therapy combined with steroid pulse therapy.
Adult ; Central Nervous System ; Humans ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive* ; Ophthalmic Solutions ; Optic Nerve* ; Papilledema ; Prednisolone ; Retina* ; Retinal Detachment ; Retinaldehyde ; Visual Acuity

PURPOSE: To evaluate the expression of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) in response to high glucose concentration in human retinal pigement epithelial (RPE) cells. METHODS: After confluent adult human RPE cells (ARPE-19) were cultured in RPMI 1640 media containing three different concentration of glucose (5.5, 11 and 22 mmol/L) for 3, 7 and 14 days, Western blot analysis was performed. Cell pellets were lysed in lysis buffer and cell lysates were centrifused to collect supernatant fractions for quantifying protein concentration. The proteins were subjected to electrophoresis and transferred to nitrocellulose membranes. After the blots were incubated with primary and secondary antibody to VEGF or HGF consecutively, enhanced chemiluminescence and autoradiography were performed. To evaluate the relationship between protein and mRNA expression, RNA from glucose-treated RPE cells was obtained by using RNAzolB. After cDNA was obtained through reverse transcription of RNA, PCR of cDNA was performed by using primers. RESULTS: We found that incubation with different concentrations of glucose increased the protein expression of VEGF and HGF in concentration-dependent manner in RPE. At 14 days, level of VEGF protein expression was higher in 22 mmol/L glucose group than in other two groups (5.5 mmol/L or 11 mmol/L), but no differences of mRNA expression of VEGF were observed in each group. At 3 days, level of HGF protein expression was higher in 22 mmol/L glucose group than in other groups, but mRNA expression of HGF was not detected in all groups. CONCLUSIONS: These results suggest that RPE cells may participate in angiogenesis in progression of diabetic retinopathy through production of VEGF and HGF.
Adult ; Autoradiography ; Blotting, Western ; Collodion ; Diabetic Retinopathy ; DNA, Complementary ; Electrophoresis ; Glucose* ; Hepatocyte Growth Factor ; Humans* ; Luminescence ; Membranes ; Polymerase Chain Reaction ; Retinaldehyde ; Reverse Transcription ; RNA ; RNA, Messenger ; Vascular Endothelial Growth Factor A*

No abstract available.
Burns* ; Humans ; Interleukin-2*

The properties of ideal root canal sealers include the ability of sealing the total root canal system and no toxic effects to periradicular tissues. Cytotoxicity test using cell culture is a common screening method for evaluation of the biocompatibility of root canal sealers. The purpose of this study was to investigate the cytotoxic effect of newly developed resin-based sealer (Adseal 1, 2, and 3) comparing with those commercial resin-based sealers (AH26 and AH Plus), ZOE-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT) and calcium hydroxide based sealer (Sealapex). An indirect contact test of cytotoxicity by agar diffusion was performed according to the international standard ISO 10993-5. L929 fibroblast cells were incubated at 37degrees C in humidified 5% CO2-containing air atmosphere. The freshly mixed test materials were inserted into glass rings of internal diameter 5 mm and height 5 mm placed on the agar. After the 24 hrs incubation period, the decolorization zones around the test materials were assessed using an inverted microscope with a calibrated screen. A Decolorization Index was determined for each specimen. Adseal 1, 2, and 3 did not exert any cytotoxic effects, whereas AH26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, and Sealapex produced mild cytotoxicity.
Agar ; Atmosphere ; Calcium Hydroxide ; Cell Culture Techniques ; Dental Pulp Cavity* ; Diffusion ; Fibroblasts ; Glass ; Mass Screening

Neuroendocrine neoplasm of the larynx is a morphologically heterogeneous group of tumors. This unusual neoplasm includes the four different type of tumors. Paraganglioma is the best-known neural origin tumor. The epithelial origin tumor is further divided based on the degree of differentiation into well, moderately, and poorly differentiated carcinoma. The diverse biological behavior of neuroendocrine neoplasm of larynx makes an accurate diagnosis of paramount importance, since treatment depends on diagnostic accuracy. The diagnosis is based primarily on light microscopy, and immunohistochemical and/or ultrastructural investigations are needed to diagnose correctly. The mainstay of treatment of moderately differentiated neuroendocrine carcinoma of the larynx is surgery. Elective neck treatment should be performed considering the high likelihood of cervical lymph node metastases. The 5-yr survival rate approximates 50%, and 10-yr survival rate is only 30%.
Carcinoma, Neuroendocrine ; Larynx ; Light ; Lymph Nodes ; Microscopy ; Neck ; Neoplasm Metastasis ; Paraganglioma ; Survival Rate

PURPOSE: To evaluate anatomical locations and distributions of supraorbital notch and foramen using facial 3D computed tomography in the Korean adult population. METHODS: The study sample was composed of 87 adult patients with no history of trauma or ocular disease. The horizontal position of the supraorbital foramen or notch was recorded in relation to a vertical line defined by a reproducible hypothetical point, such as the nasion and mid-maxilla and the midpoint of the horizontal supraorbital plane. The distance and angle for each supraorbital foramen and notch were calculated from the defined vertical line. Furthermore, vertical distance from supraorbital plane, which was established using the highest points of both supraorbital rims, was obtained from the supraorbital foramen. RESULTS: The mean age of the 87 patients was 45.44 +/- 8.34 years (range, 30-59 years). There were 66 eyes in the supraorbital notch and 108 eyes in the supraorbital foramen. There were no distributional differences between the 2 sides. The mean horizontal distance of both types was 23.95 +/- 3.93 mm (range, 16.41-38.94 mm). The horizontal distance of male patients was longer than the female patients (25.18 +/- 4.16 mm vs. 22.63 +/- 3.19 mm, p < 0.001, based on independent t-test) and the horizontal distance of supraorbital notch was shorter than the supraorbital foramen (22.59 +/- 3.18 mm vs. 26.18 +/- 4.04 mm, respectively, p < 0.001, based on independent t-test). The mean vertical distance and mean angles of the supraorbital foramen were 3.02 +/- 1.119 mm and 6.81 +/- 2.31 degrees (degrees), respectively. CONCLUSIONS: The present study described the anatomical location of each supraorbital opening type in Korean adults. According to horizontal distance, a surgeon can avoid iatrogenic injury of the supraorbital neurovascular complex, especially during brow surgery. In addition, the anatomy can aid in targeting supraorbital neurovascular complex in cases of nerve block.
Adult ; Female ; Humans ; Male ; Nerve Block