Rumex japonicus Houttuyn are ubiquitous plants, which are found in marshes or in wet areas. The root of Rumex japonicus Houttuyn has been used for the treatment of skin diseases including fungal infections of the skin since older times in Japan and China. This study was undertaken to investigate the antifungal activity of the alcoholbenzene extract of Rumex japonicus Houttuyn in vitro. During this experiment the extracts of Rumex japonicus Houttuyn were diluted seriaIly in the Sabouraud's dextrose agar and fungal mats or fungal suspensions of isolated strains of dermatophytes were inoculated into each medium containing different concentrations of test materials. Then their growth was observed for 2 weeks or 10 days at room temperature. (countinued...)
Agar ; Arthrodermataceae ; China ; Glucose ; Japan ; Rumex* ; Skin ; Skin Diseases ; Suspensions ; Wetlands

A clinical study was made on 148 cases of cutaneous malignant tumors that visited the Department of Dermatology at Seoul National University Hospital during a 11-year period from 1973 to 1983. The results were summarized as follows: 1) The incidence of cutaneous malignant tumors in the total number of outpatients visiting the Department of Derrnatology was 0. 025g. 2) There were 90 cases of male (60. 8p,) and 58 cases of female (39. 2g). The ratio of male to female was l. 55: l. Among the 148 cases of cutaneous malignant tumors, the most frequently observed type was basal cell carcinoma, 58 cases (3j.2 p:). The next most frequent types were squamous cell carcinoma, 42 cases(28. 4g) and malignant melanoma, ]1 cases (7. 4g). We described the clinical characteristics of the patients with basal cell carcinoma, squamous cell carcinoma and malignant melanoma that have been observed.
Carcinoma, Basal Cell ; Carcinoma, Squamous Cell ; Dermatology ; Female ; Humans ; Incidence ; Male ; Melanoma ; Outpatients ; Seoul

A histopathological observation of drug-induced morbilliform eruption and erythema multiforme was made on 32 patients with drug eruption, We found that many histopathologic features of drug-induced morbilliform eruption of basal cell layer with infiltration of derrnoepidermal junction and necrosis of epidermal keratinocyte were similar to those of drug induced erythema multiforme. Therefore, it seems possible to regard the histopathologic features of druginduced morbilliform eruption and erythema multiforrne as a cornmon tissuc reaction with spectral expression.
Drug Eruptions ; Erythema Multiforme* ; Erythema* ; Humans ; Keratinocytes ; Necrosis

BACKGROUND: Although palmoplantar epidermal cysts have long been associated with develop ment following implantation of an epidermal fragment as a result of a penetrating or blunt injury, the pathogenic mechanism is still not fully understood. Since 1987, many cases have been reported in which human papillomavirus(HPV) could be associated with palmoplantar epidermal cysts. OBJECTIVE: In this study, we evaluated the clinicopathological findings of palmoplantar epidermal cysts and investigated them for the presence of HPV in order to examine the role of HPV in the pathogenesis of this disorder in Korea. METHODS: The clinical, histological, immunohistochemical studies were performed on seven cases of plantar cysts, and two cases of palmar cysts. RESULTS: No previous trauma history was seen. Histopathologically, parakeratotic nuclei, or vacuolar strutures within the keratinous mass in the cyst cavity were found. However, we could not find intracytoplasirnic eosinophilic bodies in the wall, the cyst content, or the overlying epidermis. The dyskeratotic keratinocytes were observed in 3 cases. Papillomavirus common antigens were not detected by immunohissochemical staining. CONCLUSIONS: Palmoplantar epidermal cysts in Korea have some different histological features compared to those of HPV 60-associated cases in Japan and we could not detect the immunohistochemical evidence of HPV infection in our cases.
Eosinophils ; Epidermal Cyst* ; Epidermis ; Humans ; Japan ; Keratinocytes ; Korea ; Wounds, Nonpenetrating

Ex.traosseous osteogenic sarcoma is exceedingly rare although bone formation occurs commonly in different reactive processes or neoplasms. Most of this tumors occurred in the lower extremities of middle aged people. The overall prognosis is grave and local recurrence following simple local excision possibly contributes to the poor prognosis. An agressive surgical approach including major amputation is recommended as the primary form of treatment. We have experienced a case of extraosseous osteogenic sarcoma of left gluteal region at the site of therapeutic irradiation for cervical carcinoma 16 years previously.
Amputation ; Buttocks ; Humans ; Lower Extremity ; Middle Aged ; Osteogenesis ; Osteosarcoma ; Prognosis ; Recurrence

BACKGROUND: The immortalized human keratinocyte line, HaCaT cells have been widely used as substitutes for normal epidermal keratinocytes. Recently, reconstruction of a skin equivalent using HaCaT cells showed a multilayered epithelium,but somewhat different tissue architecture as compared with normal epidermis. OBJECTIVE: In this study, using HaCaT cells we tried to reconstruct an epidermis resembling more closely to normal epidermis than the previous results. MATERIALS AND METHODS: HaCaT cells were cultured in air-liquid interface on a recently developed dermal substrated in our laboratory, de-epidermized dermis (DED) raised on fibroblast-populated collagen matrix and the result was compared with those on DED or fibroblast-populated collagen matrix alone. RESULTS: HaCaT cells on the new dermal substrate formed a multilayered epithelium with rete ridges, showing rather orderly cellular organization compared with those on fibroblast-populated collagen matrix. However, horny and granular layers were not observed contrary to normal epidermis. Immunohistochemical studies revealed that differentiation markers such as keratin 1, keratin 6 and involucrin showed the similar pattern to those in HaCaT cells cultured on fibroblast-populated collagen matrix. Markers of terminal differentiation, loricrin and filaggrin were not expressed contrary to normal epidermis. CONCLUSION: These results suggest that organotypic culture HaCaT cells on the dermal substrate combines DED with fivroblast-populated collagen matrix results in incomplete differentiation of HaCaT cells contrary to normal keratinocytes.
Antigens, Differentiation ; Collagen* ; Dermis* ; Epidermis ; Epithelium ; Humans ; Keratin-1 ; Keratin-6 ; Keratinocytes ; Skin

Lymphomatoid papulosis(LyP) is a chronic recurrent dermatosi characterized by involuting and recurring papules, plaques, and nodules showing histologic feaurs suggesting rnalignant lymphoma. On histologic ground, it is divided into two types. type A and type B. In type A, large atypical lymphocytes are the main cellular cornponent and most of these express CD 30(Ki-1) antigen, which has been p!reviously thought to be specific for Read Sternberg cells of Hodgkins disease. In type B, cerebriform mononuclear lymphocytes sirr la to those in mycosis fungoides predominates. We report two cases of lymphomatoid papulosis, type A and type B, respectively. The large atypical cells in type A LyP expressed Ki 1 antigen. Both case were treated with PUVA and the outcome of the treatment has been fair.
Antigens, CD30 ; Hodgkin Disease ; Lymphocytes ; Lymphoma ; Lymphomatoid Papulosis ; Mycosis Fungoides

Herpes zoster is a viral infection of the dorsal nerve root causing painful vesicular eruptions over the skin or mucous membrane of the affected nerve. It is less well known to the dermatologists that berpes zoster causcs bladder dysfunction when the zoster affects the lumbar and sacral spinal ganglia. The authors observed a case of herpes zoster involving the S 2~4 dermatomes, associated with bladder atony in a 58 year old male who had received rad.iotherapy and chemotherapy for his malignant Iymphoma of lymphocytic type. Conservative treatment with urinary catheterization and antibiotics resulted in complete recovery of the bladder function about 3 weeks later. He had also ichthyosiform skin lesions on his back and lower extremities which, by carefuI history and histopathological examination, proved to be acquired ichthyosis.
Anti-Bacterial Agents ; Drug Therapy ; Ganglia, Spinal ; Herpes Zoster* ; Humans ; Ichthyosis* ; Lower Extremity ; Lymphoma* ; Male ; Middle Aged ; Mucous Membrane ; Skin ; Urinary Bladder ; Urinary Bladder, Neurogenic* ; Urinary Catheterization ; Urinary Catheters

We investigated the distribution of S-100 protein positive dendritic cells in the skin lesions with tuberculoid sturcture. For this study, we selected the paraffin blocks of biopsied specimens with the characteristic histopathology of lupus vulgaris (5cases), tubereulosis verrucosa cutis (1 case), lupus milaris disseminatus faciei (4 cases), and erythema induratum (7 cases). The cells were identified by immunohistochemical demonstration in paraffin sections. The results were as follows: 1. S-100 protein positive dendritic cells were regularly visualized in all lesions examined. 2. S-100 protein positive dendritic cells appeared usually between the lymphohistiocytic infiltrates around the tuberculoid granulomas in contrast to the cells of monocyte-macrophage system which were within the granulomas. And they appeared occasionally (e.g. in a case of lupus vulgaris) between epitheloid cells in the granulomas. 3. S-100 protein positive dendritic cells were more numerous in the granulomatous lesions which showed the well-formed tuberculoid sturcture. From these results, we suggested the S-100 protein positive dendritic cells act as accessory cells in the pathogenesis of the granulomatous lesions by the delayed type hypersensitivity.
Dendritic Cells* ; Erythema Induratum ; Granuloma ; Hypersensitivity ; Lupus Vulgaris ; Paraffin ; S100 Proteins ; Skin*

We report a case of pigmented spindle cell nevus occurred in a 4-year-old girl. The lesion was well-demarcated, 2.5 x 3mm sized black macule on the left thenar area. The histopathologic findings of excisional biopsy specimen revealed the prociferation of uniform spindle-shaped pigmented melanocytes at the dermoepidermal junction and sharply definded lateral margins.
Biopsy ; Child, Preschool ; Female ; Humans ; Melanocytes ; Nevus, Spindle Cell*