1.Progression of renal diseases and antihypertensive treatment.
Korean Journal of Medicine 2004;66(1):4-10
No abstract availalbe.
2.Progression of renal diseases and antihypertensive treatment.
Korean Journal of Medicine 2004;66(2):108-119
No abstract available.
3.Genomic analysis of Mycobacterium foruitum by pulsed-filed gel electrophoresis.
Tae Yoon LEE ; In A DO ; Sung Kwang KIM
Yeungnam University Journal of Medicine 1995;12(2):366-385
Epidemiological studies are important in both the prevention and treatment of mycobacterial infections. This study was initiated to establish the pulsed-field gel electrophoresis (PFGE) method, which are not yet extensively studied. The most apprpriate restriction endonucleases included Dral, AsnI, and XbaI. The optimal PFGE condition was different according to the enzymes used. Two stage PFGE was performed, in case of DraI first stage was performed with 10 seconds of initial pulse and 15 seconds of findA pulse, while the second stage was performed with 60 seconds of initial pulse and 70 seconds of final pu',se. The electrophoresis time for DraI-PFGE was 14 hours for each stage. Electrophoresis was performed for 22 hours, in case of XbaI, with 3 seconds of initial pulse and 12 seconds of final pulse. Electrophoresis was performed for 22 hours, in case of AsnI, with 5 seconds of initial pulse and 25 seconds of final pulse. In all cases the voltage of the electrophoresis was maintained constantly at 200 voltage. Standard mycobacterial strains, which included Mycobacterium bovis BCG, M. tuberculosis, and M. fortuitum, could not be differentiated by PFGE analysis. PFGE analysis was performed to differentiate 9 clinically isolated M. fortuitum strains using AsnI. All M. fortuitum strains showed different genotypes except 2 strains. Cluster analysis divided M. fortuitum strains into 2 large groups. PFGE analysis was performed to further differentiate M. fortuitum isolates using XbaI. The undifferentiated 2 M. fortuitum strains showed different PFGE patterns with Xba I. Cluster analysis of the XbaI-PFGE patterns showed more complex grouping than AsnI-PFGE patterns, which showed that XbaI-PFGE analysis was better than AsnI-PFGE in M. fortuitum genotyping. The top dissimilarity values of AsnI-PFGE and XbaI-PFGE were 0.74 and 0.75, respectively. This value was higher than that of arbitrarily primed polymerase chain reaction (AP-PCR) analysis and lower than that of restriction fragment length polymorphism (RFLP) analysis. This suggested that PFGE can be used as a supportive or alternative genotyping method to RFLP analysis.
DNA Restriction Enzymes
;
Electrophoresis*
;
Electrophoresis, Gel, Pulsed-Field
;
Epidemiologic Studies
;
Genotype
;
Mycobacterium bovis
;
Mycobacterium*
;
Polymerase Chain Reaction
;
Polymorphism, Restriction Fragment Length
;
Tuberculosis
4.Cloning and Sequencing of the phoA Gene which is Regulated by the phoP-phoQ operon in Pathogenic Enteric Bacteria.
Yeungnam University Journal of Medicine 1995;12(2):237-245
The DNA fragment containing the phoA of Klebsiella pneumoniae was cloned into pACYC184. The size of the insert. was 4.0 kb and the restriction map showed it contained 3 Pstl sites and 4 PvuLI sites. The nucleotide sequence of the phoA region was determined, which showed strong (80%) sequence similarity with that of Escherichia coli. This suggested that these two species are phylogenetically very close to each other.
Base Sequence
;
Clone Cells*
;
Cloning, Organism*
;
DNA
;
Enterobacteriaceae*
;
Escherichia coli
;
Klebsiella pneumoniae
;
Operon*
5.DNA Diagnosis Using Polymerase Chain Reaction.
Yeungnam University Journal of Medicine 1991;8(2):13-23
No abstract available.
Diagnosis*
;
DNA*
;
Polymerase Chain Reaction*
6.Analysis of gene products induced by phosphate starvation in enteric bacteria.
In A DOH ; Tae Yoon LEE ; Sung Kwang KIM
Journal of the Korean Society for Microbiology 1992;27(6):517-524
No abstract available.
Enterobacteriaceae*
;
Starvation*
7.Familial Congenital Muscular Torticollis: A Case Report
The Journal of the Korean Orthopaedic Association 1986;21(5):955-958
Hereditary theory was first proposed by Joachimsthal in 1905. However, a family history of congenital muscular torticollis is uncommon. This is a report of two female children who are interrelated and have congenital muscular torticollis. They are sisters. No environmental factor could be identified that would predispose the the children to congenital muscular torticollis. In this family, it is apparent that genetic factors are influencing the development of this condition. The cause of congenital muscular torticollis is unknown, but heredity may play a more important role than has previously been supected.
Child
;
Female
;
Heredity
;
Humans
;
Siblings
;
Torticollis
8.Fine needle aspiration cytology of malignant epithelial mesothelioma of the peritoneum.
Korean Journal of Cytopathology 1991;2(1):43-50
A case of malignant epithelial mesothelioma of the peritoneum diagnosed by fine needle aspiration cytology is described. The smear showed many individually scattered or clustered large round malignant epithelial cells intermingled with relatively small nonneoplastic mesothelial and mesenchymal cells. Papillary configurations with thick fibrous core were also seen. The malignant cells were virtually reminiscent of reactive mesothelial cells but they were larger in size and had more prominent nucleoli and more frequent binucleated or multinucleated cell formations than reactive mesothelial cells. The characteristic features of malignant cell of mesothelioma compared with the metastatic adenocarcinoma were relatively uniform cellular size, prominent round nucleoli, large round vesicular nuclei with finely granular chromatin pattern, smooth nuclear membrane, abundant glassy cytoplasm rather than bubbly mucin-containing cytoplasm and fuzzy cell border.
Adenocarcinoma
;
Biopsy, Fine-Needle*
;
Chromatin
;
Cytoplasm
;
Epithelial Cells
;
Mesothelioma*
;
Nuclear Envelope
;
Peritoneum*
9.The preoperative prognostic factors in chronic otitis media.
Korean Journal of Otolaryngology - Head and Neck Surgery 1993;36(3):490-497
No abstract available.
Otitis Media*
;
Otitis*
10.A clinical review of acute appendicitis.
Sung Yun LEE ; Sung Chul KIM ; Kwang Tae KIM
Journal of the Korean Surgical Society 1991;40(1):68-76
No abstract available.
Appendicitis*