1.A Study on the Morphological Analysis of Sperm.
Jae Seung PAICK ; Seong Soo JEON ; Soo Woong KIM ; Won Jin YI ; Kwang Suk PARK
Korean Journal of Fertility and Sterility 1997;24(2):153-165
In male reproducible health, fertility and IVF (in-vitro fertilization), semen analysis has been most important. Semen analysis can be divided into concentration, motional and morphological analysis of sperm. The existing method which was developed earlier to analyze semen concentrated on the sperm motility analysis. To provide more useful and precise solutions for clinical problems such as infertility, semen analysis must include sperm morphological analysis. But the traditional tools for semen analysis are subjective, imprecise, inaccurate, difficult to standardize, and difficult to reproduce. Therefore, with the help of development of microcomputers and image processing techniques, we developed a new sperm morphology analyzer to overcome these problems. In this study the agreement on percent normal morphology was studied between different observers and a computerized sperm morphology analyzer on a slide-by-slide basis using strict criteria. Slides from 30 different patients from the SNUH andrology laboratory were selected randomly. Microscopic fields and sperm cells were chosen randomly and percent normal morphology was recorded. The ability of sperm morphology analyzer to repeat the same reading for normal and abnormal cells was studied. The results showed that there was no significant bias between two experienced observers. The limits of agreement were 4.1%~-3.8%. The Pearson correlation coefficient between readers was 0.79. Between the manual and sperm morphology analyzer, the same findings were reported. In this experiments the slides were stained by two different methods, PAP and Diff-Quik staining methods. The limits of agreement were 7.2%~-5.7% and 6.0%~-6.3%, respectively. The Pearson correlation coefficients ware 0.76 and 0.91, respectively. The limits of agreement was tighter below 20% normal forms. In the experiments of repeatability, 52 cells stained by PAP and Diff-Quik staining methods were analyzed three times in succession. Estimating pairwise agreement, the kappa statistic for the pairs were 0.76, 0.81, 0.86, and 0.75, 0.88, 0.88 respectively. In this study it was shown that there was good agreement between manual and computerized assessment of normal and abnormal cells. The repeatability and agreement per slide of computerized sperm morphology analyzer was excellent. The computer's ability to classify normal morphology per slide is promising. Based on results obtained, this system can be of clinical value both in andrology laboratories and IVF units.
Andrology
;
Bias (Epidemiology)
;
Fertility
;
Humans
;
Infertility
;
Male
;
Microcomputers
;
Semen
;
Semen Analysis
;
Sperm Motility
;
Spermatozoa*
2.Changes of natural killer cell cytotoxicity after treatment with prednisolone and interferon-alpha of chronic active hepatitis B.
Jeon Soo SHIN ; In Hong CHOI ; Kwan Sik LEE ; Kwang Hub HAN ; Se Jong KIM
Korean Journal of Immunology 1992;14(2):221-229
No abstract available.
Hepatitis, Chronic*
;
Interferon-alpha*
;
Killer Cells, Natural*
;
Prednisolone*
3.A Case of Idiopathic Gingival Fibromatosis and Hypertrichosis.
Seong Jin JEON ; Kwang KIM ; Young Soo CHAE ; Kee Suck SUH ; Sang Tae KIM
Korean Journal of Dermatology 1993;31(1):118-122
Idiopathic gingival fibromatosis is associated with other abnorma ities of which the most striking is hypertrichosis of variable degree. Gorlin and Pindborg described this condition as a distinct syndrome termed idiopathic gingival fibromatosis and hypertrichosis. We report a case of idiopathic gingival fibromatosis and hypertrichosis occuring in 34-year-old woman with enlarged gingival tissue, hypertrichosis, and enlargenment of the breasts, ears and nose. Laboratory tests including hormonal and chromosomal studies were all within normal limits. Histologically, the gingival hyperplastic tissue showed thick bundle of collagen fibers with few fibroblasts or capillaries and little or no inflammatory infiltrate.
Adult
;
Breast
;
Capillaries
;
Collagen
;
Ear
;
Female
;
Fibroblasts
;
Fibromatosis, Gingival*
;
Humans
;
Hypertrichosis*
;
Nose
;
Strikes, Employee
4.A Case of Idiopathic Gingival Fibromatosis and Hypertrichosis.
Seong Jin JEON ; Kwang KIM ; Young Soo CHAE ; Kee Suck SUH ; Sang Tae KIM
Korean Journal of Dermatology 1993;31(1):118-122
Idiopathic gingival fibromatosis is associated with other abnorma ities of which the most striking is hypertrichosis of variable degree. Gorlin and Pindborg described this condition as a distinct syndrome termed idiopathic gingival fibromatosis and hypertrichosis. We report a case of idiopathic gingival fibromatosis and hypertrichosis occuring in 34-year-old woman with enlarged gingival tissue, hypertrichosis, and enlargenment of the breasts, ears and nose. Laboratory tests including hormonal and chromosomal studies were all within normal limits. Histologically, the gingival hyperplastic tissue showed thick bundle of collagen fibers with few fibroblasts or capillaries and little or no inflammatory infiltrate.
Adult
;
Breast
;
Capillaries
;
Collagen
;
Ear
;
Female
;
Fibroblasts
;
Fibromatosis, Gingival*
;
Humans
;
Hypertrichosis*
;
Nose
;
Strikes, Employee
5.A Clinical Observation on the Ureteral Obstruction.
Korean Journal of Urology 1974;15(4):311-317
A clinica1 observation was made on the 14 inpatient cases of ureteral obstruction which were not originated from calculi or tuberculous nature in the Department of Urology, Korea University College of Medicine during the period of 1 year from Sep. 1973 to Aug. 1974, and following results were obtained: 1. As to the origin of the obstruction, 4 cases of ureteral fibrosis, 2 cases of aberrant vessel, and one congenital ureteral stricture, one ureteral tumor, one ureterocele, one periureteral fibrosis, and one case of ureteral ligation during the radical total hysterectomy. 2. As to the location of the ureteral stricture, the upper ureter in 9 cases. middle ureter in o2e case and lower ureter in 4 cases. 3. The surgical treatment employed were nephrectomy in 6 cases, pyeloplasty in 5, ureteroneocystostomy in 1, ureteral dilation in 1 and transurethral resection in 1 case.
Calculi
;
Constriction, Pathologic
;
Fibrosis
;
Humans
;
Hysterectomy
;
Inpatients
;
Korea
;
Ligation
;
Nephrectomy
;
Ureter*
;
Ureteral Obstruction*
;
Ureterocele
;
Urology
6.Clinical study of noncemented using AML femoral stem hip arthroplasty.
Seong Ho YUNE ; Kwang Jin RHEE ; June Kyu LEE ; Deuk Soo HWANG ; Dae Sik JEON
The Journal of the Korean Orthopaedic Association 1993;28(5):1495-1504
No abstract available.
Arthroplasty*
;
Hip*
7.Influence of Sodium Ascorbate on Microtensile Bond Strengths to Pulp Chamber Dentin treated with NaOCl.
Soo Yeon JEON ; Kwang Won LEE ; Mi Kyung YU
Journal of Korean Academy of Conservative Dentistry 2008;33(6):545-552
The purpose of this study was to evaluate the influence of sodium ascorbate on microtensile bond strengths of total-etching adhesive system to pulp chamber dentin treated with NaOCl. Pulp chambers of extracted human non-caries permanent molars were treated as follows: group 1, with 0.9% NaCl; group 2, with 5.25% NaOCl; group 3, with 5.25% NaOCl and 10% sodium ascorbate for 1min; group 4, with 5.25% NaOCl and 10% sodium ascorbate for 1 min and 10ml of water; group 5, with 5.25% NaOCl and 10% sodium ascorbate for 5 min; group 6, with 5.25% NaOCl and 10% sodium ascorbate for 5 min and 10ml of water; group 7, with 5.25% NaOCl and 10% sodium ascorbate for 10 min; group 8, with 5.25% NaOCl and 10% sodium ascorbate for 10 min and 10ml of water. Treated specimens were dried, bonded with a total-etching adhesive system (Single bond), restored with a composite resin(Z250) and kept for 24h at 100% humidity to measure the microtensile bond strength. NaOCl-treated group (group 2) demonstrated significantly lower strength than the other groups. No significant difference in microtensile bond strengths was found between NaCl-treated group (group 1) and sodium ascorbate-treated groups (group 3-8). The results of this study indicated that dentin treated with NaOCl reduced the microtensile bond strength of Single bond. Application of 10% sodium ascorbate restored the bond strength of Single bond on NaOCl-treated dentin. Application time of sodium ascorbate did not have a significant effect.
Adhesives
;
Ascorbic Acid
;
Bisphenol A-Glycidyl Methacrylate
;
Dental Pulp Cavity
;
Dentin
;
Humans
;
Humidity
;
Molar
;
Sodium
;
Water
8.Effects of Exogenous Extracellular Matrix Proteins on the Reattachment of Retinal Pigment Epithelial Cells.
Kwang Soo KIM ; Yu Cheol KIM ; Se Jin JEON
Journal of the Korean Ophthalmological Society 2007;48(11):1537-1547
PURPOSE: To evaluate the effect of adding exogenous extracellular matrix (ECM) proteins on the reattachment of retinal pigment epithelium (RPE) to the damaged surface of Bruch's membrane (BM). METHODS: Porcine BM explants were divided into six groups: BMs with an intact basal lamina (bl-BM) and five damaged BMs (d-BM: bare & four ECM-coated). The d-BM was coated with ECM proteins (either fibronectin, laminin, collagen IV, or all). Primary RPE sheets were plated and cultured for each group of BM explants. The attached live cells were counted and examined with a scanning electron microscope after three days, as well as at 1, 2 and 4 weeks. RESULTS: The RPE reattachment rate was highest in bl-BM and lowest in uncoated d-BM. ECM-coated groups showed a lower reattachment rate than bl-BM, but when compared with the uncoated group, the reattachment rate was significantly increased (p<0.05). ECM-exposure time did not influence the reattachment rate of any of the groups. RPE cells plated on bl-BMs and ECM-coated d-BMs attached and proliferated well and achieved confluence over time. Even though most cells were flat and large in shape, some cells revealed a good morphology with microvilli on their surface. On the other hand, only some of the RPE sheets plated on the uncoated d-BM attached loosely and most cells remained round and clumped. CONCLUSIONS: These results show that the addition of ECM proteins may increase the ability of RPE cells to reattach to the damaged BM surface, which would likely create a good morphology.
Basement Membrane
;
Bruch Membrane
;
Collagen
;
Epithelial Cells*
;
Extracellular Matrix Proteins*
;
Extracellular Matrix*
;
Fibronectins
;
Hand
;
Laminin
;
Microvilli
;
Retinal Pigment Epithelium
;
Retinaldehyde*
9.A Case of Angiosarcoma Presenting Pleural Effusion.
Kwae Soo KYUNG ; Sung Kwang JUNG ; Ho Kee YUM ; Dong Soon KIM ; Hye Kyung LEE ; Woo Ki JEON
Tuberculosis and Respiratory Diseases 1994;41(1):36-41
Angiosarcoma is a very rare malignant tumor of endothelial cell origin. We experienced a case of angiosarcoma presented with massive pleural effusion, which was considered as a metastasis from right kidney. A 44-year-old male patient was admitted due to dyspnea for one month. He had a history of transient hematuria 3 months before admission, which disappeared spontaneously Chest roentgenography showed total haziness in left hemithorax with multiple nodular shadows in right lung. Abdominal ultrasonogram showed a single heterogeneous hyperechoic mass, measuring about 7.3×7.1×6.5cm in size in the upper and mid-pole of the right kidney, involving. renal sinus. Computed tomography of the chest revealed highly enhanced multiple pulmonary and subpleural nodules with loculated pleurisy. In bronchoscopic finding, a fungating, hypervascular tumor mass was noticed at the orifice of anterior basal segment of left lower lung after removal of tenaceous mucus Pleural and bronchoscopic biopsies showed findings of angiosarcoma confirmed by immunochemical stains with factor VIII related antigen(+), laminin(+) and vimentin(+), and by characteristic electronmicroscopic findings. Massive pleural effusion was controlled with several times of pleurodesis in both pleural spaces.
Adult
;
Biopsy
;
Coloring Agents
;
Dyspnea
;
Endothelial Cells
;
Factor VIII
;
Hemangiosarcoma*
;
Hematuria
;
Humans
;
Kidney
;
Lung
;
Male
;
Mucus
;
Neoplasm Metastasis
;
Pleural Effusion*
;
Pleurisy
;
Pleurodesis
;
Radiography
;
Thorax
;
Ultrasonography
10.Postoperative Intubation Time in Patients Undergoing Open Heart Surgery.
Ka Young RHEE ; Yun Seok JEON ; Woo Sik EOM ; Sang Hwan DO ; Chong Soo KIM ; Kwang Woo KIM
Korean Journal of Anesthesiology 1997;33(4):660-663
BACKGROUND: Cardiac patients undergoing open heart surgery usually require ventilatory support that involves ICU admission in the postoperative period. We tried to find out determinants of postoperative ventilatory support time. METHODS: We reviewed the medical records of 56 open heart surgery patients retrospectively in terms of their disease, preoperative physical status, age, post-bypass arterial oxygen tension/inspired oxygen fraction (PaO2/FIO2) ratio, number of inotropics used and searched the relationship between each factor and postoperative intubation time. RESULTS: None of the factors except the number of inotropics used had an significant influence on the postoperative intubation time. CONCLUSION: In open heart surgical patients their disease, preoperative physical status, age, postbypass PaO2/FIO2 ratio do not affect postoperative intubation time rather than number of inotropics used does.
Heart*
;
Humans
;
Intubation*
;
Medical Records
;
Oxygen
;
Postoperative Period
;
Retrospective Studies
;
Thoracic Surgery*