No abstract available.

Calvarium protects the brain, the most important organ. The defect of calvarium results in not only deformity but also fatal injury from the trauma. The cranial bone defects result from 1) removal of bone flap for intracranial decompression or infection 2) fracture 3) excision of tumor 4) craniectomy for craniosynostosis. The goals of cranioplasty are to protect the brain from trauma and make the aesthetically acceptable contour. From 1990 to 1995, we experienced twelve cases of cranioplasty using autogenous bone graft; 5 cases with rib bone, 3 cases with iliac bone, 2 cases with calvarial bone, and 2 cases with rib and calvarial bones. The result was very excellent without any significant complications.
Brain ; Congenital Abnormalities ; Craniosynostoses ; Decompression ; Ribs ; Skull ; Transplants*

No abstract available.
Cerebrospinal Fluid* ; Cholesteatoma*

TGF-beta1 is a potent chemotactic factor for inflammatory cells and fibroblasts. It also stimulates the celluar source and components of extracellular matrix and the production of proteinase inhibitors. Collectively, these biologic activities lead to the accumulation and stabilization of the nascent matrix, which is vital to wound healing. The objective of this study is to investigate production of TGF-beta1 in vitro fibroblast culture in the presence of Staphylococcus enterotoxin B(SEB) and/or lipopolysaccharide(LPS) and to elucidate the role of TGF-beta1 which may be responsible for wound healing The fibroblasts were originated from facial dermis and hypertrophic scar in 26 year-old male patient. In the presence of LPS(0.0l microgram, 0.1 microgram, 1.0 microgram), SEB(0.0l microgram, 0.1 microgram, 1.0 microgram) respectively, cells(5x103ml) were cultivated in vitro. At 1, 3, and 5 days after incubation, cells were counted. Also, cells(2.5x105ml) were cultivated in EMEM with LPS(0.01, 0.1 and 1.0 microgram), SEB(0.01, 0.1 and 1.0 microgram) respectively and LPS(0.1 microgram) and SEB(0.1 microgram) in combination for 24, 48, and 72 hours respectively. Culture supernatants were harvested at 1, 2, and 3 days after incubation period and triplicate culture supernatants were pooled and TGF-beta1 was assayed in duplicate. The results were as follows. 1. In facial dermal fibroblast induced with SEB and LPS respectively or in combination, the suppression of cell proliferation occurred very significantly at 1 day after incubation, compared with the control. In SEB exposure, the production of TGF-beta1 was decreased very significantly at 1 day after incubation, compared with the control. However, in LPS, SEB and LPS exposure, the production of TGF-beta1 was increased very significantly at 1 day after incubation, compared with the control. 2. In hypertrophic scar fibroblast induced with SEB and LPS respectively or in combination, the suppression of cell proliferation did not occur at 1 day after incubation, compared with the control. In SEB and LPS exposure in combination, the production of TGF-beta1 was increased very significantly at 1 day after incubation, compared with the control. However, the production of TGF-beta1 did not occur in SEB and LPS exposure respectively. In conclusion, the concentration of bacterial toxins and the incubation period correlated with cell proliferation and production of TGF-beta1 very significantly and both fibroblasts have different phenotype each other in this regard. This data suggest that the significant production of TGF-beta1 may develope abnormal wound healing associated with tissue fibroproliferative disorder, such as hypertrophic scar and keloid formation.
Adult ; Bacterial Toxins* ; Cell Proliferation ; Cicatrix, Hypertrophic ; Dermis ; Enterotoxins ; Extracellular Matrix ; Fibroblasts* ; Humans* ; Keloid ; Male ; Phenotype ; Staphylococcus ; Transforming Growth Factor beta1 ; Wound Healing

No abstract available.
Animals ; Linoleic Acid* ; Macrophages, Peritoneal* ; Mice*

In order to study the efficacy of a 0.2% ciprofloxacin-soaked collagen shield for the treatment of Staphylococcus aureus keratitis, an experimental study was performed on 40 eyes of 20 rabbits. Twenty hours after intrastromal injection with S. aureus, the rabbits were divided into four groups: Group 1(10 eyes) was treated with a 0.2% ciprofloxacin-soaked collagen shield with an additional instillation of 0.2% ciprofloxacin drops every 30 minutes for 4 hours; Group 2 (10 eyes) was treated with a 0.2% ciprofloxacin-soaked collagen shield for 4 hours; Group 3 (10 eyes) was treated with 0.2% ciprofloxacin drops every 30 minutes for 4 hours; Group 4 (10 eyes) received BSS every 30 minutes for 4 hours as a control. Bacterial killing was quantitated by culturing corneal homogenates and calculating the number of viable bacteria (colony-forming units) per cornea. Groups 1, 2, and 3 showed significantly reduced numbers of bacteria compared with the control (p<0.01). The order of efficacy in the treatment for S. aureus keratritis were groups 1, 2 and 3, respectively, but the statistical difference was not significant among groups 1, 2, and 3(p>0.05). These results suggested that a 0.2% ciprofloxacin-soaked collagen shield may be an effective and convenient mode of therapy for S. aureus keratitis.
Bacteria ; Ciprofloxacin ; Collagen* ; Cornea ; Homicide ; Keratitis* ; Rabbits* ; Staphylococcus aureus* ; Staphylococcus*

No abstract available.
Biopsy, Fine-Needle* ; Breast*

Solid pseudopapillary tumor (SPT) is known to be cured in more than 95% of patients after surgery. However, it is reported that 7% of this tumor can recur after resection, and continued surveillance is needed after surgery for SPT. In this case report, a pancreatic mass which had been detected during a routine health checkup was followed up and surgical resection (distal pancreatectomy) was performed due to an increase in size. Postoperative pathology revealed synchronous SPT and neuroendocrine tumor (NET). At twelve months after surgery, a recurrence of the mass in the head of the remnant pancreas was noted and further surgical resection (total pancreatectomy) was performed. Postoperative pathology confirmed the diagnosis of malignant SPT. To date, there are only a few reports of the synchronous SPT and NET, and there are no reports in Korea. Therefore, we report a case of the synchronous SPT and nonfunctional NET diagnosed after surgery.

PURPOSE: The aim of this study is to compare two different gingival depigmentation techniques using an erbium:yttrium-aluminum-garnet (Er:YAG) laser and rotary instruments. METHODS: Two patients with melanin pigmentation of gingiva were treated with different gingival depigmentation techniques. Ablation of the gingiva by Er:YAG laser was performed on the right side, and abrasion with a rotary round bur on the opposite side. RESULTS: The patients were satisfied with the esthetically significant improvement with each method. However, some pigment still remained on the marginal gingival and papilla. The visual analog scale did not yield much difference between the two methods, with slightly more pain on the Er:YAG laser treated site. CONCLUSIONS: The results of these cases suggest that ablation of the gingiva by an Er:YAG laser and abrasion with a rotary round bur is good enough to achieve esthetic satisfaction and fair wound healing without infection or severe pain. Prudent care about the gingival condition, such as the gingival thickness and degree of pigmentation along with appropriate assessment is needed in ablation by the Er:YAG laser procedure.
Gingiva ; Humans ; Hyperpigmentation ; Melanins ; Pigmentation ; Wound Healing

No abstract available.
Renal Artery* ; Ureter* ; Veins*