No abstract available.
Cells, Cultured* ; Mycoplasma*

Cytokines are hormone-like proteins which mediate and regulate inflammatory and immune responses. The purpose of this study was to investigate the effect of lipopolysaccharide (LPS), Staphylococcus enterotoxin B (SEB), and Mycoplasma lysates on regulation of IL-6 and IL-8 production by human nasal fibroblasts. Primary cultured cells were incubated with LPS (1.0 ug/ml) from E.coli, SEB (1.0 ug/ml) from S.aureus, or Mycoplasma lysates (M.pneumoniae, Mp; M. fermentans, Mf; M. hominis, Mh, each 1.0 ug/ml). The culture supernatants were collected at 2, 6, and 24 hr and assessed for IL-6 and IL-8 production by enzyme-linked immunosorbent assay. The production of IL-6 in the culture supematant was downregulated by LPS, SEB, or Mycoplasma lysates. But IL-6 was upregulated by mixed exposure with Mp+LPS (2 hr), Mp+LPS+SEB (24 hr), Mf+LPS (24 hr), Mf+LPS+SEB (2 hr), Mh+LPS (24 hr), Mh+SEB (24 hr), or Mh+LPS+ SEB (24 hr). The production of IL-8 in the culture supematant was similar to that of IL-6 by same stimulants. But IL-8 was upregulated by mixed exposure with Mf+LPS+SEB (2 hr), Mh+LPS (24 hr), Mh+ SEB (24 hr), or Mh+LPS+SEB (24 hr). These studies show that costimulation of LPS or SEB with Mycoplasma whole cell lysates upregulates the production of IL-6 and IL-8.
Bacterial Toxins* ; Cells, Cultured ; Cytokines ; Enterotoxins ; Enzyme-Linked Immunosorbent Assay ; Fibroblasts* ; Humans* ; Interleukin-6 ; Interleukin-8 ; Mycoplasma* ; Staphylococcus

No abstract available.

No Abstract Available.
Prevalence* ; Ureaplasma urealyticum* ; Ureaplasma* ; Uterine Cervical Neoplasms*

No abstract available.
Femur* ; Fracture Fixation, Intramedullary* ; Tibia*

PURPOSE: The modern era of hypospadias surgery has brought functional and cosmetic results to a high level in children. However, little data are available on the outcome of adult hypospadias repairs. The objective of this study was to evaluate the results of 46 adult patients with hypospadias repairs. MATERIALS AND METHODS: One surgeon performed forty-six consecutive adult hypospadias repairs. The mean age at the time of surgery was 22.1 years (range 13 to 59). Follow up ranged from 3 months to 7 years (mean 11.3 months). We retrospectively analyzed surgical outcomes, with respect to previous repair, surgical procedure and complications. RESULTS: Of 46 adult hypospadias patients, 20 had had previous repairs and 26 had had no previous repairs. Surgical procedures consisted of 7 onlay island flap, 6 pyramid, 4 transverse island flap, 2 King, 3 MAGPI, 2 Mathieu, 3 tubularized incised plate and 1 meatal advance in the group with no previous repairs. In the group that had undergone previous repairs there were 10 Thiersch-Duplay, 2 Johanson, 2 transverse island flap, 1 MAGPI, 1 King, 1 Durham-Smith, 1 Mustard, 1 meatal advance and 1 onlay island flap. Complications developed in 11 patients from the no previous repair group, including 6 fistula, 2 stricture, 4 wound infection, 2 wound dehiscence and 1 ventral curvature, and were as follows for the previous repair group; 12 fistula, 5 stricture, 3 wound infection and 4 wound dehiscence. CONCLUSIONS: For both groups analysed, adult hypospadias repair was much less successful than childhood repair. Further studies are necessary to decrease the complications.
Adult* ; Child ; Constriction, Pathologic ; Female ; Fistula ; Follow-Up Studies ; Humans ; Hypospadias* ; Inlays ; Male ; Mustard Plant ; Retrospective Studies ; Wound Infection ; Wounds and Injuries

No abstract available.
Animals ; Linoleic Acid* ; Macrophages, Peritoneal* ; Mice*

No abstract available.
Carcinoma, Mucoepidermoid*

No abstract available.
Bacteria* ; Tetracycline*

No abstract available.
Bacteria* ; Tetracycline*