BACKGROUND: Onychomycosis is very common nail problem, so an in xpensive, quick and sensitive test is essential for screening nail specimens. Recently, there is a report of new method for diagnosing onychomycosis u.;ing KOH treated nail clippings which vri then crushed and finally stained with periodic acid-Schifft(PAS) stain (KONCPA). OBJECTIVE: We evaluate the susefulness of the new methods using KOH treated nail clippings and nail debris which were then crushed and finally stained with FS (KONPA) or chlorazol black E(KONBE), for the diagriosis of onychomycosis. METHODS: We compare different methods for diagnosing onychonycisis such as KOH stains, fungal cultures, histologic evaluation, SEM, KONCPA, KONBE, and KOHJPA. RESULTS: KONPA was proved to be more effective indentifying uiigal hyphae in comparison with conventional KOH nail scraping preparation, fungal culture, and FONBE. The positive rates of each method were 74%, 46%, 43%, and 63%, respectively. Also, KENPA proved to be more rapid and easy to perform in cotnparison to the histologic evaluation of tiail clippings and SEM. CONCLUSION: KONPA is a sensitive, quick, and readily available teled for use in clinical settings in cases that are highy siispected to be onychomycosis clinicaly, but show negative results using conventional methods
Coloring Agents ; Hyphae ; Mass Screening ; Onychomycosis*

Pulmonary adenocarcinoma of fetal type is a very uncommon tumor of the lung which simulates an early stage of lung differentiation. This is a primitive appearing epithelial tumor similar to the epithelial component of pulmonary blastoma but lacking the sarcomatous features. Since the report of Kradin et al, 8 more cases have been reported by a variety of name. These tumors are composed of glycogen-rich, non-ciliated tubular epithelial cells forming irregularly shaped tubules or arranged in a papillary pattern. A very remarkable findings of this tumor is the presence of endocrine cells which is confirmed by argyrophilia, immunohistochemistry or electron microscopy. We experienced a case of this tumor which showed hepatocytoid differentiation in addition to the characteristic histologic findings. Immunohistochemical studies performed on a resected tumor tissue showed immunoreactivity for alpha-fetoprotein, neuron-specific enolase and somatostatin, and endocrine type granules were found ultrastructurally. Although this tumor seems to have a relation with pulmonary blastoma in its histology, immunohistochemistry and ontogeny, a distinction between these should be attained because the average survival of the former group is longer as 23 months, while that of the latter is only 4 months.
Adenocarcinoma

A 25 year old man presinted with erythematous and inderated plaques on the upper and lower extermities that were preceded by insect bites while traveling in central Africa. The patient had a past histrory of Kimura's disease, asthma, and allergic rhinitis, Histologic examination revealed panniculitls showing massive eosinophilic infiltration. The dermis showed eosinopbilic infilreatiom without flame figyres. The patient responded well to oral conrticosteroids. This patient did not fit the diagnosis of eosinoplilic pannicylitis, eosinophilic cellylitis, hypereosinophilic syndrome or eosinophilic vascylitis.
Africa, Central ; Asthma ; Dermis ; Diagnosis ; Eosinophils* ; Humans ; Hypereosinophilic Syndrome ; Insect Bites and Stings ; Rhinitis, Allergic ; Skin*

No abstract available.
Allografts* ; Femur* ; Giant Cell Tumors* ; Giant Cells*

No abstract available.
Carpal Tunnel Syndrome*

Buschke-Lowenstein tumor is a rare disease in the category of designated as verrucous carcinoma characterized by its invasive downward penetration of underlying tissues in the perineum and perianal regions. Viruses, unclean sanitation and cytotoxic immune reaction have been proposed as the etiology of the tumor. However, among all the causes, recent studies have emphasized on the associaton of the tumor and human papilloma virus (HPV). Expecially, HPV also has been discovered in several cutaneous and anogenital lesions of solid organ transplant recipients. We herein report a case of Buschke-Lowenstein tumor in a renal transplant recipient with HPV 6 and 16 coinfection proved by HPV genotyping of DNA extracted from the biopsy specimen of the tumor.
Biopsy ; Buschke-Lowenstein Tumor* ; Carcinoma, Verrucous ; Coinfection ; DNA ; Human papillomavirus 6 ; Humans ; Papilloma ; Perineum ; Rare Diseases ; Sanitation ; Transplantation* ; Transplants

We present a 40-year-old woman who had a well circumscribed, pea sized red-brown glistening erosive papule on the foreskin of clitoris for four months which had been resistant to various forms of treatment. Histopathologic examination revealed flattened epidermis with lozenge keratinocytes and watery spongiosis, In the upper dermis, dense bandlike infiltration of plasma cells, endothelial proliferation, and capillary dilatation were noted. Peroxidase-antiperoxidase stain showed polyclonal pattern of reaction in the cytoplasm of plasma cells.
Adult ; Capillaries ; Clitoris ; Cytoplasm ; Dermis ; Dilatation ; Epidermis ; Female ; Foreskin ; Humans ; Keratinocytes ; Peas ; Plasma Cells ; Vulvitis*

A 14-year-old boy and his 16-year-old elder brother, who had generalized tinea corporis for 1 and 3 years respectively, developed multiple discrete, non tender soft tumors on the scalp, forehead, neck and extremities. The boys were well nourished and had no systemic diseases. Histopathologic examination of the subcutaneous nodules revealed a well encapsulated granuloma containing lobulated granules characteristic of mycetoma; these granules consisted of septated fungal hyphae with vesicles. The dermatophyte isolated from the tumors was identified as Trichophyton schoenleinii.
Adolescent ; Arthrodermataceae ; Extremities ; Forehead ; Granuloma ; Humans ; Hyphae ; Male ; Mycetoma ; Neck ; Scalp ; Siblings* ; Tinea ; Trichophyton*

An immunoperoxdase technique in electron microscopy was used to investigate the ultrastructural site of keratinolytic proteinase (KPase) of M crosporum cans in sections of skin from guinea pigs infected with the same organism. Ultrastructurally, the KPase was present only in the cell walls of the invading dermatophytes as a continuous deposition of the electron-dense reaction product on the inner and outer aspects of the cell wall of the fungal hyphae without deposition in the keratin surrounding the invading hyphae. Our results suggest that the KPase may not play an absolute role in the invasion of dermatophytes into keratinized tissue in vivo.
Animals ; Arthrodermataceae ; Cell Wall ; Guinea Pigs* ; Guinea* ; Hyphae ; Immunoenzyme Techniques* ; Microscopy, Electron* ; Microsporum* ; Skin* ; Tinea

No abstract available.
Hernia, Inguinal* ; Humans