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BACKGROUND: The human keratinocyte can synthesize interleukin 6 (IL-6) under certain conditions, and the IL-6 synthesis is inhibited by interleukin 4 (IL-4) in the human monocyte. OBJECTIVE: To find out what kind of stimulating agents can induce the IL-6 production and whether IL-4 affects the production of IL-6 in the human cultured keratinocytes. METHODS: We stimulated the keratinocytes with either lipopolysaccharide (LPS), fetal bovine serum (FBS), human recombinant interferon-gamma(IFN-gamma) to induce the IL-6 production, and treated the keratinocytes, which stimulated either with 10% FBS or human recombinant IFN-gamma, with human recombinant IL-4. RESULTS: The LPS stimulation resulted in no increase of IL-6 levels in the keratinocyte supernatants. When the keratinocytes were stimulated either with 1%, 5%, 10% FBS with or without 5 microgram/ml LPS, significantly increased amounts of IL-6 were detected. The level of IL-6 in the keratinocytes treated with the human recombinant IFN-gamma increased, too. The human recombinant IL-4 downregulates the secretion of IL-6 by the keratinocytes which were activated either with 10% FBS or human recombinant IFN-gamma. CONCLUSION: We have shown that it is possible to induce the IL-6 synthesis by stimulating the keratinocytes and that human recombinant IL-4 profoundly inhibits the synthesis of IL-6. So we suggest that there may be a cytokine network which regulates the primary immune response in the skin.
Humans* ; Interleukin-4* ; Interleukin-6* ; Interleukins* ; Keratinocytes* ; Monocytes ; Skin

10.The effects of monokines on neutrophil activation.

Su Yeon KIM ; Hyung Sik KANG ; Jeong Sang LEE ; Suk Ran YOON ; Choong Eun LEE ; Kwang Ho PYUN

Korean Journal of Immunology 1991;13(2):135-142

1.Stress and Psychoneuroendoimmunology.

2.Interation of Immune and Nervous Systmes.

3.Regulation of transforming growth factor-beta1 synthesis by nurine T lymphocytes and its effect of IgA antibody response.

4.The roles of interleukin-6 in fibrosis.

5.Effects of IL-6 on the proliferation and ICAM-1 expression of keratinocytes.

6.Regulation mechanisms of inflammatory response induced by Fc? stimulation in human monocytes and monocytic cell line, thp1.

7.Studies on the role of IgE-receptor in the pathogenesis of minimal change nephrotic syndrome and development of new immuno-therapeutic regimen.

8.Differentiation of human B lymphocytes by B cell stimulating cytokines.

9.Effects of Interleukin 4 on the Production of Interleukin 6 in Human Keratinocytes.

10.The effects of monokines on neutrophil activation.

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