1.In vitro growth of candida albicans on several resilient dinture liners.
Chae Heon CHUNG ; Kwang Won KIM ; Dong Ki KIM ; Zang Hee LEE
The Journal of Korean Academy of Prosthodontics 1993;31(1):19-27
No abstract available.
Candida albicans*
;
Candida*
2.Clinical Observation in 26 Cases of Indigenous Malaria in 1995.
Kwang Hee KIM ; Chae Seung LIM
Korean Journal of Medicine 1997;52(5):577-583
OBJECTIVE: In Korea, control activities against the endemic malaria due to Plasmodium vivax had been done in 1960s. It is now widely accepted in Republic of Korea that indigenous malaria stopped its endemicity in the mid-1970s. But a vivax malaria case without history of being abroad was recognized in 1993. In addition to this case, 20 cases of indigenous malaria were detected in 1994. And they were progressively increased to about 80 cases in 19%. We report these recently developed cases. METHODS: In a 7-month period between June and December 1995, high fever patient were examined. Patients having a narcotic drug injection or those who had blood transfusion were excluded. Laboratory studies including blood cell count, peripheral blood smear and abdominal sonogaphy were taken. RESULTS: Over 3-month period from July through September 19%, 26 cases of new indigenous malaria were diagnosed. In a blood smear, plasmodium vivax was diagnosed. CONCLUSION: The twentysix new indigenous malaria cases occured. Because all the detected patients had no history of visiting endemic countries, they were not imported cases. Vivax malaria in the present patients can be contracted by infected mosquitos which came across the militarized zone (DMZ). Officially speaking, DMZ is a 4 km wide between the north and south borders, Hut the width is much narrower in many parts. However, we do not have any information on the malaria situation in the north Korea. In order to evaluate a significance of the recent indigenous malario occurrence, a surveillance system should be operated in these areas.
Blood Cell Count
;
Blood Transfusion
;
Culicidae
;
Democratic People's Republic of Korea
;
Fever
;
Humans
;
Korea
;
Malaria*
;
Malaria, Vivax
;
Plasmodium vivax
;
Republic of Korea
3.A Case of Cutaneous Polyarteritis Nodosa.
Hyun Chul CHAE ; Kyung Hee CHOI ; Mi Soo AHN ; Ji Sub OH ; Sin Kwang KANG
Journal of the Korean Pediatric Society 1995;38(10):1422-1428
No abstract available.
Polyarteritis Nodosa*
5.Genome Size Constraint in Replication and Packaging of Turnip Yellow Mosaic Virus.
Hui Bae KIM ; Kwang Hee CHAE ; Tae Ju CHO
Journal of Bacteriology and Virology 2014;44(2):188-196
Turnip yellow mosaic virus (TYMV) is a spherical plant virus that has a single 6.3 kb positive strand RNA as a genome. Previously, we have made the recombinant TYMV construct containing a 0.7 kb eGFP gene or a 1.8 kb GUS gene. The genomic RNAs from these constructs were efficiently encapsidated. To examine in more detail whether size constraint exists for replication and packaging of TYMV, we have inserted into the TY-GUS an extra sequence derived from either eGFP or GUS. We also made a recombinant containing RNA1 sequence of Flock house virus. These TYMV recombinants were introduced into Nicotiana benthamiana leaves by agroinfiltration. Northern blot analysis of the viral RNAs in the agroinfiltrated leaves showed that the genomic RNA band from the recombinant TYMV became weaker as longer sequence was inserted. The result also showed that the efficiency of genomic RNA encapsidation decreased sharply when an extra sequence of 2.2 kb or more was inserted. In contrast, the recombinant subgenomic RNA containing an extra sequence of up to 3.2 kb was efficiently encapsidated. Overall, these results show that size constraint exists for replication and encapsidation of TYMV RNA.
Blotting, Northern
;
Genome
;
Genome Size*
;
Plant Viruses
;
Product Packaging*
;
RNA
;
RNA, Viral
;
Tobacco
;
Tymovirus*
6.N-terminal Extension of Coat Protein of Turnip Yellow Mosaic Virus has Variable Effects on Replication, RNA Packaging, and Virion Assembly Depending on the Inserted Sequence.
Kwang Hee CHAE ; Doyeong KIM ; Tae Ju CHO
Journal of Bacteriology and Virology 2016;46(1):13-21
Turnip yellow mosaic virus (TYMV) is a non-enveloped icosahedral virus composed of 20 kDa single coat proteins. In this study, we modified the TYMV coat protein (CP) ORF by inserting an oligonucleotide linker corresponding to T7, HSV, Tat, (Arg)9, or (RxR)4 peptide at the 5'-end of the CP ORF and examined its effect on replication, RNA packaging, and virion assembly. The results showed that the constructs containing (Arg)9 and (RxR)4 sequences were barely capable of replication. The TYMV constructs containing T7 and Tat peptide produced virions that co-migrated with wild-type virions. However, the insertion of T7 and Tat sequences impaired genomic RNA (gRNA) accumulation and packaging, respectively. When only the CP gene was expressed, CPs with (Arg)9 or (RxR)4 successfully produced virus-like particles whose mobility was comparable to that of wild type. In the case of CP having a HSV tag, the virion band was not detected, although a sufficient amount of CP was produced. This indicates that CP with the HSV tag failed to assemble into virions. Overall, the results suggest that TYMV replication, RNA packaging and virion assembly are strongly influenced by the insertion sequence.
Animals
;
Brassica napus*
;
Capsid Proteins
;
Ecthyma, Contagious
;
Product Packaging*
;
RNA*
;
Tymovirus*
;
Virion*
7.Psychosocial Distress and Its Related Factors among Clerical Public Officers.
Kwang Hee CHO ; Dong Bae LEE ; Young Chae CHO
Korean Journal of Occupational and Environmental Medicine 2007;19(1):26-37
OBJECTIVES: This study was performed to determine the levels of psychosocial distress among clerical public officers to reveal the various related factors. METHODS: A self-administered questionnaires was administered between Dec. 5th, 2005 and Jan. 31st, 2006, to 1,005 individuals in the service of general administration located in Daejeon City. The survey items included the subjects'socio-demographic and job-related characteristics, health-related behavior, degree of job demand, job autonomy, social support in work, type A behavior pattern, locus of control, sense of self-esteem, and psychosocial distress. RESULTS: The results showed that 24.8% of subjects were categorized as high risk stress, 64.9% as latent stress, and 10.3% as normal. The level of psychosocial distress was influenced by various sociodemographic characteristics, such as personality traits (type A behavior pattern, locus of control, selfesteem) and whether performing health-related behavior or not, as well as according to job-related characteristics such as rank in work, job career, job specifications, job demand, job autonomy, social support in work. CONCLUSIONS: The effective strategy for stress reduction among governmental employees requires additional programs focusing on innovated job specifications and managed personality traits.
Internal-External Control
;
Psychology
;
Questionnaires
8.Bond-strength of several metal-meramic alloys and meneered-porcelain.
Kwang Hoon LEE ; Young Bum CHO ; Chae Heon CHUNG ; Hee Jung KIM
The Journal of Korean Academy of Prosthodontics 2011;49(3):191-196
PURPOSE: In this study, we evaluated the bond-strength between a few metal ceramic-alloys and veneered-porcelain and found if the bond strength of metal-ceramic alloy with lower gold content than the presently used gold alloy was high enough. MATERIALS AND METHODS: For this study, after plasticizing porcelain only for gold alloy, metal specimen was fabricated using Ni-Cr alloy and gold alloy with different gold content. Three point flexural test were performed to measure their bond-strength. RESULTS: The bonding strength of Group 1 to porcelain was 40.62 +/- 3.32 MPa, which was the highest (P<.05). In sequence of decreasing value, Group 2 (75%) was 37.47 +/- 1.57 MPa, Group 3 (52%) 35.85 +/- 1.48 MPa, Group 4 (51.5%) 35.04 +/- 1.34 MPa, Group 5 (32%) 33.17 +/- 1.62 MPa, Group 6 (10%) 30.75 +/- 1.21 MPa. Bonding strength of Group 3 and Group 4 to porcelain did not show statistically significant difference with comparison to that of Group 5 (Duncan's test, P>.05), while there was a significant difference between that of Group 2, Group 3 and Group 4 and that of Group 6 (Duncan's test, P<.05). The bonding strength between gold alloy and porcelain increased according to the content of gold. In all experimental groups showed higher value than 25 MPa, which is the least value recommended by ISO 9693. CONCLUSION: In all groups, bonding strength was higher than 25 MPa, which is the least value of ISO9693. Therefore, it is considered that metal gold alloy with low gold content is clinically usable.
Alloys
;
Dental Porcelain
;
Plastics
9.Simultaneous bilateral tubal pregnancies following in - vitro fertilization and embryo transfer.
Hee Chul KIM ; Bum Chae CHOI ; Jae Hoon LEE ; Kwang Moon YANG ; Soo Jung HONG ; Hye Sun KIM ; Seog Hun KIM ; Chang Heon KIM ; Inn Soo KANG
Korean Journal of Obstetrics and Gynecology 2000;43(4):751-754
Over the last 20 years, the frequency of multiple pregnancy has increased mainly because of the introduction of exogenous pituitary gonadotropins in the treatment of infertility. Since the advent of assisted reproductive technology, the concern about ectopic implantation of embryos has increased dramatically and it continues to be a major complication of in vitro fertilization and embryo transfer (IVF-ET). Bilateral tubal pregnancy is the least common type of ectopic implantation of two embryos. Of all extrauterine pregnancies, 1:725~1:1580 are bilateral1,2. Simultaneous tubal pregnancies have been reported in natural cycles, recently, after ovulation induction, in-vitro fertilization2,3. The high incidence of ectopic pregnancy associated with IVF-ET continues to be a problem and frequencies of between 2.4 and 12.4% have been only a few reports of simultaneous bilateral tubal pregnancies following IVF-ET4. This paper describes a case of a simultaneous bilateral tubal pregnancy after IVF-ET in a 33 year old patient. The diagnosis was confirmed by diagnostic laparoscopy performed 25 days after embryo transfer(sixth week of gestation), which revealed bilateral tubal pregnancy. Bilateral salpingectomy was performed. With a review of the literature on this topic, diagnostic aspects and treatment options are discussed.
Adult
;
Diagnosis
;
Embryo Transfer*
;
Embryonic Structures*
;
Female
;
Fertilization in Vitro
;
Fertilization*
;
Gonadotropins, Pituitary
;
Humans
;
Incidence
;
Infertility
;
Laparoscopy
;
Ovulation Induction
;
Pregnancy
;
Pregnancy, Ectopic
;
Pregnancy, Multiple
;
Pregnancy, Tubal*
;
Reproductive Techniques, Assisted
;
Salpingectomy
10.A case of primary hepatic angiosarcoma.
Sang Jin PARK ; Chae Yoon CHON ; Hyun Seung SHIN ; Hee Yong MOON ; Kwang Hyub HAN ; Yung Myung MOON ; Jin Kyung KANG ; In Suh PARK ; Chan Il PARK
Korean Journal of Medicine 1993;45(6):818-823
No abstract available.
Hemangiosarcoma*