This study was designed to answer the question; (1) How does the DNA ploidy pattern change in hepatocarcinogenesis? (2) How does the nuclear morphology change in hepatocarcinogenesis? Diethylnitrosamine(DEN) (16.5 mg per kg) was subcutaneously injected to female Sprague-Dawley rats(150~200g) by weekly interval for 30 weeks. DNA ploidy and parameters of nuclear morphology were measured by image analyser(IBAS 200, Kontron, FRG). The DNA ploidy pattern was divided into three basic patterns(diploid, polyploid, and aneuploid modes). In 8 cases of saline-injected control rats, the DNA histograms showed all polyploid pattern. Inhepatocyte nodules(hyperplastic nodules), DNA diploidy was the most frequent pattern, being followed by polyploid and aneuploid DNA patterns, contrast to hepatocelular carcinomas in which polyploid DNA pattern was most frequently noted being followed by diploid and aneuploid DNA pattern. Although the nuclei of hepatocytes in hepatocyte nodules and hepatocellular carcinomas were larger and more pleomorphic than those of normal hepatocytes, they were as same as those of normal hepatocytes in regard to nuclear hyperchromasia. DNA content, which was increased in hepatocarcinogenesis, was significantly related to the nuclear area.
Female ; Humans ; Carcinoma, Hepatocellular

Pancreatic pseudocysts are a common complication of both acute and chronic pancreatitis. But fistular formation or spontaneous perforation of a pancreatic pseudocyst through adjacent organs is rare. Even rarer is bleeding through the fistula between stomach and pancreatic pseudocyst with splenic artery pseudoaneurysm. Therapeutic modalities for ruptured splenic artery pseudoaneurysm with pancreatic pseudocyst include surgical correction and/or angiographic intervention likely splenic artery embolization. However, there seem to be a debate about which treatments are superior. We experienced a case of massive gastric bleeding through a gastrocystic fistula that was controlled surgically by distal pancreatectomy and gastric wedge resection. We report the case with a literature review.
Aneurysm ; Aneurysm, False ; Fistula ; Hemorrhage ; Pancreatectomy ; Pancreatic Pseudocyst ; Pancreatitis, Chronic ; Splenic Artery ; Stomach

BACKGROUND AND OBJECTIVES: This study was performed to evaluate coronary flow reserve (CFR) the relation between CFR and exercise capacity and the effects of verapamil, on the CFR in patients with hypertrophic cardiomyopathy (HCMP) using transthoracic doppler echocardiography (TTE). SUBJECTS AND METHODS: 21 patients with HCMP, and 29 normal controls, were enrolled. The mean diastolic coronary flow velocity (CFmv), and time velocity integral of diastolic coronary flow (CFtvi), were measured in the distal left anterior descending coronary artery, both before, and after dipyridamole infusion. The CFR was defined as the post-dipyridamole CFmv/baseline CFmv ratio. Treadmill tests (TMT) were performed, on 14 patients, to evaluate the relationship between exercise capacity and CFR. The CFR in 7 patients was measured before, and after, verapamil administration. There were no differences in baseline hemodynamics for the 21 patients with HCMP, compared to the 29 normal controls. The baseline CFmv, and Cftvi, in the 21 patients, were significantly higher than those of the controls (0.40+/-0.09 vs 0.31+/-0.06 m/sec, p<0.001, 0.25+/-0.07 vs 0.16+/-0.04 m, p<0.001), while the CFR was lower (2.01+/-0.42 vs 3.06+/-0.39, p<0.001). The CFR showed negative correlation with the baseline CFmv in patients (correlation coefficient=-0.522, p=0.015). In 14 patients, who performed TMT, neither the CFR nor CFmv correlated with the maximal exercise time. In 7 patients, verapamil administration did not increase CFR. CONCLUSION: Because of elevated resting CFmv, and CFtvi, the CFR in patients with HCMP, were reduced. Reduced exercise capacity in patients with HCMP cannot be explained by the reduced CFR. Treatment with verapamil did not increase the CFR.
Cardiomyopathy, Hypertrophic* ; Coronary Vessels ; Dipyridamole ; Echocardiography, Doppler* ; Exercise Test ; Hemodynamics ; Humans ; Verapamil

BACKGROUND AND OBJECTIVES: Little is known about the relationship between the apolipoprotein (apo) B-100, or the apo B-100/apo A-I ratio, and coronary artery disease (CAD). The aim of this study was to investigate this association. SUBJECTS AND METHODS: Our study was carried out on 194 patients who had undergone elective coronary angiography (CAG), but had received no lipid-lowering medication. Patients with acute myocardial infarction were excluded. Stenosis of >or=50% in 1 or more coronary arteries was classified as CAD (+). RESULTS: HDL-C and apo A-I were significantly higher in females than in males (p=0.009 and 0.036). In our population we found that the apo A-I, HDL-C and the apo B-100/apo A-I ratio were significantly related to CAD (p=0.001, 0.006, and 0.007 respectively). In the male group (n=111), the apo B-100/apo A-I ratio was the only parameter statistically significant to CAD after correcting for age, diabetes mellitus and hypertension. Whereas, in the female group (n=83), the apo B/apo A-I ratio, apo B-100/apo A-I ratio, apo B-100, nonHDL-C, triglyceride, apo B, total cholesterol and low-density lipoprotein cholesterol were all significantly related to CAD (p=0.002, 0.003, 0.003, 0.007, 0.007, 0.009, 0.012 and 0.012 respectively). Of these parameters only the apo B-100/apo A-I ratio was significantly related to CAD in both female and male group. CONCLUSION: The apo B-100/apo A-I ratio is an useful indicator for discriminating between CAD (+) and CAD (-).
Apolipoprotein A-I ; Apolipoprotein B-100 ; Apolipoproteins B ; Apolipoproteins* ; Cholesterol ; Constriction, Pathologic ; Coronary Angiography ; Coronary Artery Disease* ; Coronary Disease ; Coronary Vessels* ; Diabetes Mellitus ; Female ; Humans ; Hypertension ; Lipoproteins ; Male ; Myocardial Infarction ; Triglycerides