1.Analysis of DNA Ploidy Patterns and Nuclear Morphometry in Diethylnitrosamine Induced Hepatocyte Nodules and Hepatocellular Carcinoma of Rats.
Chan CHOI ; Myung Kwan KIM ; Kwan Mook CHAE ; Eun Cheol KIM ; Hyung Bae MOON
Korean Journal of Pathology 1993;27(3):226-234
This study was designed to answer the question; (1) How does the DNA ploidy pattern change in hepatocarcinogenesis? (2) How does the nuclear morphology change in hepatocarcinogenesis? Diethylnitrosamine(DEN) (16.5 mg per kg) was subcutaneously injected to female Sprague-Dawley rats(150~200g) by weekly interval for 30 weeks. DNA ploidy and parameters of nuclear morphology were measured by image analyser(IBAS 200, Kontron, FRG). The DNA ploidy pattern was divided into three basic patterns(diploid, polyploid, and aneuploid modes). In 8 cases of saline-injected control rats, the DNA histograms showed all polyploid pattern. Inhepatocyte nodules(hyperplastic nodules), DNA diploidy was the most frequent pattern, being followed by polyploid and aneuploid DNA patterns, contrast to hepatocelular carcinomas in which polyploid DNA pattern was most frequently noted being followed by diploid and aneuploid DNA pattern. Although the nuclei of hepatocytes in hepatocyte nodules and hepatocellular carcinomas were larger and more pleomorphic than those of normal hepatocytes, they were as same as those of normal hepatocytes in regard to nuclear hyperchromasia. DNA content, which was increased in hepatocarcinogenesis, was significantly related to the nuclear area.
Female
;
Humans
;
Carcinoma, Hepatocellular
2.Correlation between Reverse Redistribution and Subendocardial Myocardial Infarction Observed in Myocardial Contrast Echocardiography.
Sung Eun KIM ; Jun KWAN ; Won Sick CHOE
Korean Journal of Nuclear Medicine 2000;34(3):228-233
PURPOSE: The aim of this study is to better understand the pattern and nature of reverse redistribution (RR) in myocardial perfusion imaging. MATERIALS AND METHODS: In consecutive 20 acute myocardial infarction (MI) patients, frequency of RR was correlated with that of subendocardial MI that was detected by myocardial contrast echocardiography (MCE). RR was judged to be present when there was more than one grade of worsening in perfusion at 24 hr delayed images compared with the initial rest images. MCE evaluated the significant lack of opacification in the subendocardial myocardium relative to the subepi-cardial myocardium to suggest the subendocardial MI. Kendall's nonparametric correlation coefficiency was calculated. RESULTS: Concordant cases were 15 of 20 (75%) and correlation was statistically significant (p=0.0285). CONCLUSION: Our results suggested that RR was correlated with MCE-detected nontransmural MI.
Echocardiography*
;
Humans
;
Myocardial Infarction*
;
Myocardial Perfusion Imaging
;
Myocardium
;
Perfusion
3.Histomorphologic Changes of Small Intestinal Mucosa after Irradiation in Rats.
Chan Hwan KIM ; Eun Sook CHANG ; Keon Young KWON ; Kwan Kyu PARK ; Ok Bae KIM
Korean Journal of Pathology 1999;33(9):639-651
Inadvertent application of ionizing radiation, a valuable tool in diagnostic radiology and radiotherapy, results in injury and death of adjacent normal cells, inducing gene mutations or even producing latent cancers. Captopril, an angiotensin I converting enzyme (ACE) inhibitor, has been reported to prevent the structural and functional changes in variable organs, such as lung and kidney, from radiation injury in different experimental animal models. An experiment was carried out to elucidate the radiation-induced histomorphologic changes of small intestine, especially jejunum, and to determine whether captopril can reduce or prevent the radiation-induced injuries in jejunum. Twenty-six healthy Sprague-Dawley rats were used. Experimental group (n=24) was divided into two large groups: the first one (n=16) was treated with two different single dose (9 Gy, 17 Gy) irradiation only and was sacrificed at 12 hours and at 8 weeks following irradiation; the second one (n=8) received captopril 500 mg/l per oral continuously after same doses of irradiation and was sacrificed at 8 weeks. The control group (n=2) was maintained on a stock diet in a same period of experimental group and sacrificed coincidentally. On light and electron microscopy, the 9 Gy and 17 Gy 12 hours groups revealed frequent apoptosis and necrosis but extremely decreased mitotic figures of the crypt cells. However, the 9 Gy and 17 Gy 8 weeks groups and the combined irradiation with captopril groups showed extremely reduced apoptosis and necrosis with increased mitotic figures. There was good correlation between experimental groups in apoptotic count and mitotic count (p<0.05). In the 9 Gy and 17 Gy 12 hours groups, the mucosal surface was focally or diffusely fragmented and the villi were slightly to moderately distorted. Collagen deposition was very mild and confined to the lower portion of the lamina propria. The 9 Gy and 17 Gy 8 weeks groups showed more severe mucosal surface fragmentation even with foci of erosion, short and distorted villi, and more intense collagen deposition. In contrast, the combined irradiation with captopril groups revealed complete regeneration of the mucosal surface epithelium and absent collagen deposition. These findings suggest that the acute radiation injuries to small intestine occur principally in the mucosal crypt cells. Captopril, the ACE inhibitor, might provide a useful intervention in the radiation injuries of intestinal mucosa.
Animals
;
Apoptosis
;
Captopril
;
Collagen
;
Diet
;
Epithelium
;
Intestinal Mucosa*
;
Intestine, Small
;
Jejunum
;
Kidney
;
Lung
;
Microscopy, Electron
;
Models, Animal
;
Mucous Membrane
;
Necrosis
;
Peptidyl-Dipeptidase A
;
Radiation Injuries
;
Radiation, Ionizing
;
Radiotherapy
;
Rats*
;
Rats, Sprague-Dawley
;
Regeneration
4.Current Immune Status of Children and Pregnant Women in Korea(I).
Eun Jung KIM ; Ju Kwan PARK ; Chang Hwi KIM ; Sang Jhoo LEE
Journal of the Korean Pediatric Society 1989;32(5):620-629
No abstract available.
Child*
;
Female
;
Humans
;
Pregnant Women*
5.A Short-Term In-Vitro Chemosensitivity Test for Ovarian Cancer: Nuclear Damage Assay.
Young Tak KIM ; Kwan Ja JI ; Yong Man KIM ; Joo Hyun NAM ; Jung Eun MOK
Korean Journal of Gynecologic Oncology and Colposcopy 1997;8(1):47-54
No abstract available.
Ovarian Neoplasms*
6.Light Electron Microscopic Study in Rat Livers Following Cadmium Chloride Administration.
Kwan Kyu PARK ; Young Ho KIM ; Kun Young KWON ; Eun Sook CHANG ; Moo Ung CHANG
Korean Journal of Pathology 1992;26(1):28-39
This study was carried out to investigate the light and electron microscopic findings of the livers of rats after an intraperitoneal injection of cadmium chloride. The Sprague-Dawley rats were intraperitoneally injected with cadmium chloride dissolved in water, once a day for three days. These animals were sacrificed at 1, 3, 8, and 24 hr after the last injection. Control groups of the rats were also sacrificed in the same manner. The liver was extirpated and examined by both light and electron microscopy. The results obtained are as follows: The parenchyma of the liver shows focal neutrophilic infiltration and spotty necrosis. The hepatocytes show fatty change, ballooning degeneration, swelling of the endoplasmic reticulum and mitochondria, increase numbers of secondary lysosomes and residual bodies. Focal patic venules and sinusoids of the liver are congested. The Kupffer cells are increase in number. Therefore, it can be concluded that the cadmium is directly acted to hepatocytes resulting in cellular injuries and deposits in the fat droplets of the cytoplasm of the hepatocytes, not Ito cells as previously suggeted.
Rats
;
Animals
7.Reexpansion pulmonary edema: report of 1 case.
Dong Kwan KIM ; Myung Sub HYUN ; Eun A LEE ; Seung Kyun LIM
The Korean Journal of Thoracic and Cardiovascular Surgery 1993;26(9):718-721
No abstract available.
Pulmonary Edema*
8.Hemodynamics and Pathophysiology of Hypertension in Different Stages of Chronic Renal Parenchymal Disease.
Yonsei Medical Journal 1986;27(3):178-199
No abstract available.
Adolescent
;
Adult
;
Comparative Study
;
Female
;
Hemodynamics*
;
Human
;
Hypertension/etiology*
;
Hypertension/physiopathology
;
Kidney Failure, Chronic/physiopathology*
;
Kidney Failure, Chronic/surgery
;
Kidney Transplantation
;
Male
;
Middle Age
;
Nephrectomy
9.Assesment of tubal patency : Transvaginal sonosalpingography(TV-SSG) versus hysterosalpingography(HSG).
Jae Young LEE ; Byung Young LEE ; Eun Kwan LEE ; Byung Ik LEE ; Hyun Chan KIM
Korean Journal of Obstetrics and Gynecology 1993;36(7):1962-1966
No abstract available.
Equidae*
10.Renal amyloidosis (a case report).
Kwan Kyu PARK ; Kun Young KWON ; Eun Sook CHANG ; Sung Bae PARK ; Hyun Chul KIM
Korean Journal of Nephrology 1991;10(4):625-631
No abstract available.
Amyloidosis*