OBJECTIVE:To observe therapeutic efficacy of adjuvant treatment of Xuebijing injection for severe acute pancreati-tis. METHODS:80 cases of severe acute pancreatitis were randomly divided into control group and observation group with 40 cas-es in each group. Control group was given symptomatic and supportive treatment,and observation group was additionally given in-travenous injection of Xuebijing injection 100 ml,twice a day,on the basis of control group. The levels of TNF-α,IL-6 and hs-CRP,organ failure were compared between 2 groups before and after treatment. RESULTS:There was no statistical significance in the levels of TNF-α,IL-6 and hs-CRP in 2 groups before treatment(P>0.05);after treatment,the levels of TNF-α,IL-6 and hs-CRP in observation group was significantly lower than control group and before treatment,with statistical significance (P<0.05). The proportion of renal function,respiration function and circulating failure in observation group were significantly lower than in control group,with statistical significance (P<0.05). CONCLUSIONS:Adjuvant treatment of Xuebijing injection for se-vere acute pancreatitis can significantly reduce the level of inflammation in the body and reduce organ damage.

Objective To study the effects of intra-bone marrow injection of donor bone marrow cells in combination with low dose radiation on the immunologic reaction of composite tissue allotransplantation.Methods The inbred SD rats were chosen as donors and inbred Wistar rats as recipients. Overall 40 recipients were classified into 4 groups randomly after allogeneic leg transplantation: group A received transplantation only; group B irradiation in the sublethal level (4.5 Gy?2 at a 4-h interval) and fludarabine (50 mg/kg, i.p.); group C, bone marrow cells were directly injected into the intra-bone marrow cavity of the recipients; group D, using a combination of the injection of fludarabine (50 mg/kg, i.p.), irradiation (4.5 Gy?2, at a 4-h interval) and injection of donor bone marrow cells. The rejection of grafts was observed. 120 days after induction of tolerance the mixed lymphocyte reaction (MIR) and skin grafting were examined to confirm tolerance status. To determine graft-versus-host disease (GVHD), rats in tolerance status were also histologically examined. Results As compared with other groups, mean rejection time and mean survival time of limb allografts were prolonged obviously in group D. Donor-specific tolerance was confirmed in all limb allograft recipients in group D by skin grafting and by MLR, and no signs of GVHD were also histologically examined. Conclusion Using a combination of injection of fludarabine, irradiation in the sublethal level and donor bone marrow cells, we have induced donor-specific immunological tolerance in allogeneic limb transplantation in rats without using any immnosuppressants after the operation.

Objective To investigate inhibitory effects of Sanguisorba officinalis L.on biofilms of MRSA41577.Methods Congo red agar method and crystal violet semi-quantitative method were used to detect the biofilms-forming ability of tested strains; TTC assay was used to detect inhibitory effects of Sanguisorba officinalis L.on biofilms formation and mature biofilms of MRSA41577,as well as effects of Sanguisorba officinalis L.in combination with vancomycin on mature biofilms of MRSA41577.Results Sanguisorba officinalis L.showed significant inhibitory both on biofilms formation and mature biofilms, minimum inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) of biofilms formation were 1 mg/mL and 8 mg/mL,MIC of mature biofilms was 4 mg/mL.The sensitivity of mature biofilms to vancomycin was greatly increased when Sanguisorba officinalis L.was combined with vancomycin with subinhibitory concentrations.Sanguisorba officinalis L.at 1/4 MIC can inhibit mature biofilms when combined with vancomycin at 4 μg/mL, while vancomycin didn't show inhibitory effects on mature biofilms when concentrations were below 64 μg/mL. Conclusion Sanguisorba officinalis L.has significant inhibitory on biofilms formation, the mechanism may be related to Sanguisorba officinalis L.destroyed biofilms and make vancomycin penetrate into the biofilms to finish the bactericidal activity.

Intracerebral hemorrhage (ICH) is the second most common type of stroke,and its fatality is high.The baseline hematoma volume and hematoma growth are the predictive factors for the poor outcome of the patients.Previous studies have shown that surgical evacuation of hematoma can reduce the hematoma volume and improve the outcome.However,several recent randomized controlled trials of craniotomy hematoma evacuation for ICH and a Meta-analysis have shown that it is no more beneficial than conservative treatment.The previous evidence of minimally invasive evacuation of hematoma for the treatment of ICH has suggested that it has potential benefits for patients with ICH; however,further research is needed to confirm it.

Objective To investigate the antimicrobial and antioxidant activity of the water extract and ethanol extract of the powder of G.lucidum.Analysis and comparison of two kinds of antibacterial and antioxidative effects of the extracts.Methods G.lucidum powder was prepared by using a pulverizer and extracted with water and ethanol respectively.The inhibitory effects of water extract and alcohol extract of G.lucidum on the tested strains were determined by TTC method.The antioxidant activities of aqueous extract and ethanol extract of G.lucidum were determined by DPPH and FRAP.Results The minimum inhibitory concentration ( MIC) of the aqueous extracts and ethanol extracts of G.lucidum were 32 mg/mL and 16 mg/mL, respectively.The MIC for S.Aureus respectively was 64 mg/mL and 32 mg/mL, the MIC for M.luteus respectively was 32 mg/mL and 8 mg/mL, MIC for B.terom respectively was 64 mg/mL and 8 mg/mL, The MIC of B.subtilis respectively was 32 mg/mL and 8 mg/mL.The antioxidant activity of G.lucidum powder extract and ethanol extract showed good antioxidant activity, the scavenging radical capacities on DPPH of the water extract and ethanol extract were 56.22%and 20.67% at a concentration of 2 mg/mL ( P<0.5 ) , the FRAP value respectively were 3.52 and 3.77 mmol/mL. Conclusion The powder of G.lucidum has the effective antimicrobial activity and antioxidant activity.The two kinds of extracts extract of G.lucidum had good antibacterial activity and antioxidant activity.The ethanol extract of G.lucidum powder antimicrobial effect and total antioxidative ability in water extracts, aqueous extract of Ganoderma lucidum granules on DPPH free radical scavenging rate better than that of ethanol extract.

Objective To summarize the experience of clinical diagnosis and treatment for recurrence and progress of relieved myastbenia gravis after thymectomy. Methods 22 recurrent and progressive after relieved pa-tients with myasthenia gravis who underwent thymectomy were retrospectively analyzed. The remission therapy was conducted with combined glucocorticoid and anticholinesterase and its effectiveness was estimated. Results It was 1,17,4 as better Osserman scale Ⅰ , Ⅱ , Ⅲ respectively before operation but 15,6,1 as better Osserman scale Ⅱ , Ⅲ, Ⅳ respectively in recurrence and progress of relieved myasthenia gravis after thymectomy besides 6 with myas-thenic crisis. Complete remission and partial remission were gained in 9 patients and 12 patients respectively. There was 1 hospital-death. Conclusions Recurrence and progress can occur in any patient of relieved myasthenia gravis after thymectomy. Bulbar myasthenia gravis is usually presented as dysphagia. Reasonable administration of glucocor-tieoid could improve majority of recurrence and progress of relieved myasthenia gravis after thymectomy but responses poorly to the anticholinesterases.

Baicalein (BAI) is an effective bactericide. The antibacterial activity and mechanism experiments were carried out by determining conductivity and content of macromolecules of membrane penetrability, the oxidative respiratory metabolism and protein synthesis changes and the inhibition of DNA topoisomerase activities. Electrical conductivity and the number of large molecules of BAI increased 2.48% and 1.8%, respectively, than that of the control. However, the membrane integrity did not destroyed by BAI directly. With BAI treatment, inhibition rates of activities for SDH and MDH were 56.2% and 57.4%, respectively, demonstrating that BAI could inhibit cell respiratory. After treated with BAI for 20 h, the total soluble content of proteins decreased by 42.83%. Moreover, the activities of DNA topoisomerase I and II were inhibited completely by 0.2 mmol x L(-1) BAI. These results indicated that BAI had obvious antibacterial activity on Staphylococcus aureus. The mechanism is that it could affect bacterial membrane penetrability, inhibit protein synthesis and influence SDH, MDH and DNA topoisomerase I and II activities to exert its antibacterial functions.

Objective To study the removal effect of Moringa seedprotein on turbidity water. Methods The protein of Moringaoleifera seed was extracted by salting out and salting out methodand the protein concentration of Moringa oleifera seed wasdetermined by Coomassie blue staining;The removal effect of Moringa seed protein on turbidity water was determined by coagulation test.The contents of COD, ammonia nitrogen and nitrate nitrogen in the water were determined to determine the effect of Moringa seed protein on water quality. Results The experimental results showed that Moringa oleifera seed protein has good removal effect on high and medium turbidity water, and its removal effect is in a dose - dependent manner.The removal rate of 7 mg/L of Moringa seed protein to high and medium turbidity water reached 92.25 ％ and 64.71 ％ respectively. But the removal efficiency of low turbidity water was less than 7 mg/L, the removal rate of low turbidity water was only 31.91％.The results of determination of COD, ammonia nitrogen and nitrate nitrogen showed that the Moringa seed protein did not increase the content of organic matter in the water while removing turbidity effectively. Conclusion Moringa oleifera seed protein has a certain removal effect on turbidity water, among which the removal effect of high and medium turbidity water is strong, and the removal effect of low turbidity water is poor.Moringa oleifera seed protein had little effect on water quality.

Objective To investigate the effects of adiponectin (APN) on proliferation,migration and tube formation of RF/6A cells,and explore the effects of APN on choroidal and retinal angiogenesis.Methods Well cultured RF/6A cells were randomly divided into the control group and three groups with different concentrations of recombinant adiponectin (5 pg · mL-1,50 pg · mL-1,500 pg · mL-1) for 1 hour.After 24 hours,cell proliferation,migration and tube formation were detected by MTT assay,wound scratch assay and seeding cells in matrigel,respectively.Results The cell proliferation in all APN groups was weaker than that of control group (P ＜ 0.05),the cell migration area (pixels) of all APN groups was significantly smaller than that of the control group (P ＜ 0.05),and the number of tube formation of all APN groups was significantly less than the control group (P ＜ 0.05).Cell proliferation,migration and tube formation decreased along with the increase of APN concentration.Conclusion APN can obviously inhibit the angiogenesis process of RF/6A cells.This inhibitive effect indicates the protective role of APN in choroidal and retinal angiogenesis.

AIM: To investigate the inhibitory effect of Co-SZ eye drops on apoptosis of lens epithelial cells(LEC) induced by H_2O_2 and to study the cellular and molecular mechanisms.METHODS:(1) All lenses of Sprague-Dawley rats were incubated with H_2O_2 and Co-SZ eye drops.The apoptosis rates of LEC were determined by TUNEL method.The changes of LEC ultrastructure and the formation of apoptotic body were observed by electron microscopy.(2) Bovine LEC were incubated with H_2O_2 and Co-SZ eye drops.The inhibitory of LEC apoptosis was detected by MTT after incubation.The changes of fractional DNA content in LEC were detected by flow cytometry(FCM).[Ca~(2+)]i,cAMP and cGMP of LEC were determined by spectrofluoremeter and radioimmunoassay,respectively.RESULTS: The LEC apoptosis rates in Co-SZ eye drops group were decreased significantly compared with H_2O_2 group by TUNEL.The ultrastructure changes in LEC of Co-SZ eye drops group were lighter than that in H_2O_2 group.The LEC apoptosis rates of Co-SZ eye drops group were dose-dependently decreased significantly compared with H_2O_2 groups via MTT assay.LEC apoptosis induced by H_2O_2 was inhibited by Co-SZ eye drops,and showing dose-dependent.The DNA contents in LEC of Co-SZ group were increased.The [Ca~(2+)]i and cAMP in Co-SZ group were decreased obviously.The cGMP was increased.CONCLUSION: The LEC apoptosis induced by H_2O_2 was inhibited by Co-SZ eye drops.The mechanism of apoptosis inhibition by Co-SZ eye drops maybe contribute to the increase in DNA content.The signal transduction mechanisms are related to the decrease in [Ca~(2+)]i and cAMP and the increase in cGMP.