Wound repair is a complex process involving multiple disciplines, cell factors, cell types, and signaling pathways. In recent years, many researches showed that circulating fibroblasts (cFbs) deriving from bone marrow and residing in peripheral blood, played a role in wound repair by migrating to wound sites, differentiating into fibroblasts and myofibroblasts, and secreting cytokines, extracellular matrix, chemokines, growth factors, and antigen-presenting molecules, etc. cFbs also involve in three phases of inflammation, proliferation, and remodeling in wound repair of diabetes, ulcers, burns and other wounds, which indicate that they could be a potential treatment for wound repair. This article aims to review the latest development in circulating fibroblasts and wound repair.

OBJECTIVE: The aim of this study was to explore the theoretical framework of cells and the forms of osteogenesis in the mechanism by which demineralized dentin matrix (DDM) induces osteogenesis. METHODS: A total of 24 New Zealand rabbits were used in this study. A total of 4 erector spinae bags were created in each animal. A total of 3 erector spinae bags were implanted with DDM by random selection, whereas the remaining one erector spinae bag was not implanted with DDM. The rabbits were sacrificed after 1, 2, 3, 4, 8, 12, 16, and 20 weeks, and the samples were obtained. The samples were examined by hematoxylin-eosin (HE), tartrate-resistant acid phosphatase (TRAP), and immunohistochemical staining to identify the mesenchymal stem cells, osteoblasts, chondrocytes, and osteoclasts. RESULTS: The results of HE staining showed that in the third week, cartilage- and bone-like matrices, as well as the osteoblast-like cells, were observed. The results of immunohistochemical staining showed that the expressions of CD44, alkaline phosphatase (ALP), and collagen Ⅱ were statistically significant 
(P<0.05). CONCLUSIONS DDM has good histocompatibility and osteoinduction. In addition, induced ectopic osteogenesis mode mainly occurs in the endochondral bone.
Animals ; Bone Matrix ; Dentin ; Osteoblasts ; Osteogenesis ; Rabbits ; Tooth Demineralization

Adult ; Female ; Humans ; Magnetic Resonance Imaging ; Neuroimaging ; methods ; Pituitary Neoplasms ; diagnosis

【Objective】 To analyze the effects of different fortified feeding methods on nutritional metabolism and growth rate of preterm very low birth weight infants (VLBWI), in order to provide new clues for improving the prognosis of the preterm infants. 【Methods】 A total of 115 cases of premature VLBWI admitted to Department of Neonatology, The First Affiliated Hospital of Kunming Medical University from January 2019 to December 2020 were included in this study, and were divided into fortified breastfeeding group (HFM group), mixed feeding group, and premature formula feeding group (PF group) based on their feeding methods. The effects of different feeding methods on the nutritional metabolism and growth rate of premature VLBWI were analyzed. 【Results】 1) The hospitalization time of infants in the HFM group was shorter than that in PF group and mixed feeding group (t=7.185, 6.924, P<0.05). 2) The proportion of necrotizing enterocolitis (NEC) in the HFM group during hospitalization was lower than that in the PF group (P<0.05); the proportions of late onset septicemia(LOS) and extra uterine growth restriction(EUGR) in the HFM group during hospitalization were lower than those in the PF group (χ²=5.030, 4.147, P<0.05); the proportion of LOS was lower than that of the mixed feeding group(χ²=6.589, P<0.05). 3) During hospitalization, the proportions of abdominal distension, bloody stools and increased eosinophils in the HFM group were lower than those in the PF group (P<0.05), which in mixed feeding group was lower than those in PF group (Fisher exact test, P<0.05). 4) At discharge, the weight and length growth rate of the HFM group were higher than those of the mixed feeding group (t=3.722, 0.425, P<0.001) and the PF group (t =6.015, 0.496, P< 0.001). 【Conclusion】 Fortified breastfeeding can more effectively increase the growth rate of VLBWI in premature infants, improve nutritional metabolism, reduce complications and adverse feeding reactions related to premature infants, and is safer and more effective.

Objective: To study the lignans in the leaves of Magnolia denudata. Methods: The constituents were separated and purified by silica gel, Sephadex LH-20 and semi-preparative HPLC. The structures were elucidated on the basis of spectroscopic data analysis. All compounds were evaluated for their inhibitory activity against LPS-activated NO production in RAW 264.7 cell model in vitro. Results: Two tetrahydrofuran-type lignans and three dihydrobenzofuran-type lignans were isolated from 95% ethanol extract of M. denudate. Their structures were identified as (7S,7'R,8S,8'S)-3,4,5-trimethoxy-3',4'-methylenedioxy-7,7'-epoxylignan (1), veraguensin (2), liliflol A (3), liliflol B (4) and 5-methoxyliliflol B (5). Conclusion: Compound 1 is a new compound and named as magnodatin A. Compound 5 is a new natural product. The 13C-NMR spectroscopic data of compounds 3-5 are reported for the first time. Compound 5 exhibited weak inhibitory effect on NO production in LPS-induced macrophages with a inhibiton rate of 48.7% at 50 μmol/L.

Objective To study the chemical constituents from the leaves of Magnolia delavayi. Methods The chemical constituents were isolated and purified by column chromatography on silica gel, Sephadex LH-20, MCI, and HPLC. Their structures were identified based on spectroscopic data. Results Fourteen compounds were isolated from 95% ethanol aqueous extract of M. delavayi and the structures were identified as (2E)-3,7,11-trimethyl-2,10-dodecadien-l,6,7-triol (1), (2E,6E)-3,7,11-trimethyl-2,6-dodecadien-l,10,11- triol (2), loliolide (3), pinoresinol (4), syringaresinol (5), medioresinol (6), eudesmin (7), phillygenin (8), coniferaldehyde (9), 3,4,5-trimethoxycinnamyl alcohol (10), indole-3-carboxaldehyde (11), indole-3-ethanol (12), 3,4-dimethoxy-benzoic acid (13), and 3,4-dimethoxyphenol (14). Conclusion All compounds are isolated from this plant for the first time. In addition, the 1H- and 13C-NMR spectroscopic data are reported for the first time in the current study.

Imaging diagnosis of intracranial vertebral artery dissection aneurysm (VADA) is difficult due to its deep location and complex lesion structure. Conventional MRI is difficult to accurately observe and evaluate VADA, while high resolution MRI (HRMRI) has high spatial and tissue resolution, and vascular wall imaging technology can directly display the structural and pathological changes of the vessel wall, which plays an important role for the diagnosis of VADA and the evaluation of prognosis. The application progresses of HRMRI in VADA was reviewed in this article.

Abstract Emotion ability has substantial influence on individuals survival and development as well as mental health. Emotion ability development disorder has become a prominent performance of hearing impaired children, which has attracted the attention of researchers at home and abroad. From the four aspects of emotion recognition, emotion understanding, emotion expression and emotion regulation, this paper sorts out and comments on the previous studies, puts forward the intervention measures in the light of the concrete problems existing in the development, and proposes some suggestions for the curtent study limitations, in order to provide fundamental evidance for promotion of emotion ability of hearing impaired children.

Abstract There are many problems in emotion ability development of hearing impaired children, such as emotion recognition, emotion expression obstacle, emotion understanding limitation and emotion regulation impropriety, etc., which seriously affect their social adaptation and mental health. Making systematic and individualized intervention programs can effectively promote the development of emotion ability of hearing impaired children. Based on the analysis of the characteristics and influencing factors of the emotion ability development of hearing impaired children, this paper reviews the existing intervention studies, and proposes future research directions in view of the deficiencies of existing studies, in order to provide reference for the research on emotion ability intervention of hearing impaired children.

OBJECTIVE： To study the antimicrobial activity of ampelopsin combined with 8 kinds of antibiotic against Pseudomonas aeruginosa （PA） in vitro. METHODS： Chessboard trace dilution method was used to determine minimum inhibitory concentration （MIC） of ampelopsin combined with ceftriaxone， cefoperazone/sulbactam， piperacillin/tazobactam， cefoperazone， ciprofloxacin， amikacin， piperacillin and cefepime to PA strain ATCC27853 and 7 isolated strains PA135， PA216， PA276， PA281， PA291， PA314 and PA319. Fractional inhibitory concentration index （FICI） was used to evaluate its effects of drug combination. Clinically isolated strain PA319 were taken as target strain and then divided into normal control group， ampelopsin alone group， antibiotics alone group and ampelopsin+antibiotic combination group. Using MIC of ampelopsin and antibiotics during drug combination as active concentration， the number of colonies cultured for 0， 4， 8， 12 and 24 h was counted， and the time-sterilization curve was drawn. RESULTS： For above 8 kinds of strains， MIC of ampelopsin alone was 128-256 mg/L； FICI of ampelopsin combined with ceftriaxone， cefoperazone/sulbactam， piperacillin/tazobactam， cefoperazone， ciprofloxacin， amikacin， piperacillin and cefepime to 8， 8， 7， 6， 4， 4， 6 and 6 strains were equal to or lower than 1， respectively. In time-antibacterial curve， compared with antibiotics alone， the number of colonies decreased by 2.65， 2.30， 0.42， 0.47， 0.53， 1.19， 1.74， 1.04 lgCFU/mL respectively after ampelopsin combined with ceftriaxone， cefperazone/sulbactam， piperacillin/tazobactam， cefoperazone， ciprofloxacin， amikacin， piperacillin and cefepime. CONCLUSIONS： Ampelopsin combined with ceftriaxone and cefoperazone/sulbactam show better antibacterial effect on PA.