【Objective】 To explore the psychological experience of blood donors with blood donation-related vasovagal reaction (DRVR), so as to provide basis for improving the quality of blood donation services. 【Methods】 The real experience of 9 DRVR blood donors was analyzed using the phenomenological research method in qualitative research. 【Results】 The occurrence of DRVR caused distinct psychological conflict among blood donor. Blood donors lacked knowledge about blood donation and expected professional evaluation and continuous support. The occurrence of DRVR was an important reason for blood donor lapsing. 【Conclusion】 By resolving the distinct psychological conflicts of DRVR blood donors, establishing DRVR early warning evaluation and feedback system, as well as strengthening the construction of support system, blood collection and supply institutions can create a good humanistic and social environment for sustainable development of voluntary blood donation.

Bardet-Biedl syndrome (BBS) is a rare genetic disease caused by ciliary structure abnormality or dysfunction. To date, more than 21 BBS genes (BBS1 - 21) have been reported to independently cause the disorder. Although the cellular functions of BBS proteins are not yet fully understood, model organisms have revealed that such proteins are involved in ciliary functions and intracellular transport. Among the 21 BBS genes, BBS7 is unique in that its product is a subunit of the BBSome and can directly interact with the BBS chaperonin complex. Previous studies using animal models showed that BBS7 mutation can cause similar phenotypes to human patients, and human disease caused by BBS7 variants are special and more complex. This article reviewed recent progresses on BBS7.
Animals ; Bardet-Biedl Syndrome ; genetics ; Humans ; Mutation ; Proteins ; genetics

OBJECTIVETo investigate the prevalence and distribution of HCV genotypes and the clinical effect of interferon-alpha combined with ribavirin treatment in chronic hepatitis C patients in Kunming.

METHODS60 patients were divided into two groups based on drug therapies: PEG-interferon-a plus ribavirin treatment group for HCV 1b and interferon-a plus ribavirin treatment group for non-HCV-1b. Serum ALT levels and HCV RNA quantitations of the patients were detected during treatment and follow-up.

RESULTSThe HCV genotypes of 60 patients were determined by type specific probe assay, and five different types were found. Their overall prevalence were 21.7% for type 1b, 5% for type 2a, 16.7% for type 3a, 48.3% for type 3b, and 8.3% for type 6a. Sustained viral response rates for PEG-interferon treatment group were 46.1%, for interferon treatment group were 74.4%. The abnormal rate of serum ALT after the treatment had no significant difference between HCV-1b and non-HCV-1b patients (P>0.05). All patients with early viral responses got sustained viral response.

CONCLUSIONHCV-3b is the most dominant genotype in Kunming. The effect of PEG-interferon-a plus ribavirin treatment for genotype 1b is unsatisfactory. The early viral response is a good predictor for the responses to antiviral therapy in chronic hepatitis C patients.

Adult ; Aged ; Antiviral Agents ; pharmacology ; therapeutic use ; Drug Therapy, Combination ; Female ; Follow-Up Studies ; Genotype ; Hepacivirus ; genetics ; Hepatitis C, Chronic ; drug therapy ; Humans ; Interferon-alpha ; therapeutic use ; Male ; Middle Aged ; RNA, Viral ; Ribavirin ; therapeutic use ; Young Adult

Abstract Unhealthy eating behaviors among adolescents are common, and psychological behavior problems are prominent. The paper examines the status and associations underlying unhealthy eating behaviors and psychological behavior problems in adolescents from border multi ethnic areas, and further discusses the importance of research in border area involving multi ethnic adolescents, and highlights its significance in the context of constructing healthy schools in border areas,so as to provide a basis for promoting effectively healthy school environments in border areas of China.

Objective: To further improve the proficiency and accuracy of physical diagnosis of medical students. Methods: On March 16th, 2018, a random questionnaire survey were conducted and Excel used to investigate and analyze the needs of junior medical students for the teaching of physical diagnosis from the perspectives of topic selection of micro-lecture, presentation, teaching hours etc.. Results: A total of 174 questionnaires were distributed and were all took back. In the survey, 139 students (79.89%) hoped to apply micro-lecture to the teaching of physical diagnosis, 132 students (75.86%) thought that the proper time of micro-lecture for each physical examination item should be 2-5 minutes, 150 students (86.21%) expected to learn micro-lecture of physical diagnosis via WeChat. Physical examination items selected mostly by students were on heart, chest and nervous system. Conclusion Theoretically and technologically speaking, it is feasible to make micro-lecture of physical diagnosis. Therefore, we should actively carry out the micro-lecture of physical diagnosis to meet students' learning demands and improve their operation skills of physical diagnosis.

AIM: To study the chemical constituents of the roots of Asparagus cochinchinensis (Asparagaceae). METHODS: The compounds were isolated with Diaion HP20, silica gel, and ODS chromatography, and their structures were determined on the basis of chemical methods, HR-ESI-MS, and 1D- and 2D-NMR techniques. RESULTS: Seven compounds were isolated from the n-butanol fraction of the roots of A. cochinchinensis, and their structures were elucidated as (25S)-26-O-β-D-glucopyranosyl-5β-furostan-3β, 22α, 26-triol-12-one-3-O-β-D-glucopyranoside (1), (25S)-26-O-β-D-glucopyranosyl-22α-methoxy-5β-furostan-3β, 26-diol-12-one-3-O-β-D-glucopyranoside (2), (25S)-26-O-β-D-glucopyranosyl-5β-furostan-3β, 22α, 26-triol (3), (25S)-26-O-β-D-glucopyranosyl-5β-furstan-3β, 22α, 26-triol-3-O-β-D-glucopyranoside (4), (25S)-26-O-β-D-glucopyranosyl-5β-furostan-3β, 22α, 26-triol-3-O-α-L-rhamnopyranosyl-(1, 4)-β-D-glucopyranoside (5), (25S)-5β-spirostan-3β-ol-3-O-α-L-rhamnopyranoside (6), and (25S)-5β-spirostan-3β-ol-3-O-β-D-glucopyranoside (7). CONCLUSION Compounds 1 and 2 were two new furostanol saponins.
Asparagus Plant ; chemistry ; Molecular Structure ; Plant Extracts ; chemistry ; Plant Roots ; chemistry ; Saponins ; chemistry ; isolation & purification

Spinocerebellar ataxia type 2 (SCA2) is a rare autosomal dominant progressive degenerative disease of the nervous system, which is characterized by a progressive cerebellar syndrome associated with saccadic eye scan, peripheral neuropathy, cognitive disorders, and other multisystem features. The gene predisposing to SCA2 has been mapped, which encodes the ataxin 2 protein. A CAG repeat expansion in the coding region of ATXN2 gene can cause extension of polyglutamine chain in the protein. This paper reviews recent progress made in the research on SCA2 in regard to its clinical features, pathology, etiology, pathogenesis and treatment.
Animals ; Ataxin-2 ; genetics ; Humans ; Spinocerebellar Ataxias ; etiology ; genetics ; pathology ; therapy

OBJECTIVE: In order to make a basis for the treatment of nasal sinuses of children and the correlated disease, we investigate the incidence rate of nasal sinusitis of children and explore the correlation between nasal sinusitis of children and CT in Kunming. METHOD: Two thousand one hundred and fourteen healthy children and 1535 children with sinusitis were selected randomly. Form whom were undergone nasal sinus computer tomography for different medical reasons. The incidence rates of nasal sinusitis in different age-groups were calculated and statistically analyzed. RESULT: The incidence rate of sinusitis was highest in 4 to 8 years old. There was moderate to high consistency between clinical diagnosis and nasal CT results (P<0.01). CONCLUSION These results demonstrate that the key stage of children's sinusitis prevention would be school-age children, and nasal CT scanning is helpful to evaluate the clinical condition.
Adolescent ; Child ; Child, Preschool ; China ; epidemiology ; Female ; Humans ; Incidence ; Infant ; Male ; Sinusitis ; epidemiology

OBJECTIVETo evaluate the efficacy of the implantable loop recorder (ILR) in establishing symptom-rhythm correlation in patients with unexplained syncope.

METHODSImplantable loop recorders (ILR, Reveal Plus(9526), Medtronic Inc.) were implanted in 10 patients [aged 14 - 78 (41 +/- 22) years, 6 female] with unexplained syncope from October 2002 to May 2005. Syncopal episodes were (4.5 +/- 1.4) patients.

RESULTSDuring the monitoring period [8 - 21 (15.3 +/- 3.6) months], there were 24 times syncopal episodes in 6 patients. A total of 211 arrhythmia events were documented by ILR in 7 patients and symptom-rhythm correlation could be established in these 7 patients. In 2 patients, there were no recurrent syncopes and no arrhythmia events could be recorded. In 1 patient, syncope was caused by reasons other than arrhythmia.

CONCLUSIONILR is useful in determining the presence or absence of an arrhythmia during symptoms of syncope when conventional diagnostic testing, such as electrocardiogram, Holter monitoring, and/or external loop recording, is inconclusive.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Arrhythmias, Cardiac ; complications ; diagnosis ; Electrocardiography, Ambulatory ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Syncope ; diagnosis ; etiology

OBJECTIVEThis study aimed to evaluate the effects of the microarc oxidation surface on cell morphology and cytoskeleton.

METHODSPure titanium with a diameter of 15 mm and a thickness of 1 mm was divided into four groups: grooved surfaces (group G); sandblasted surfaces (group SB); grooved microarc oxidation surfaces (group GMAO); and sandblasted microarc oxidation surfaces (group SBMAO). Osteoblast cells were cultured in each group. The morphology and proliferation of the cells on the titanium surface were observed by scanning electron microscope (SEM). The cytoskeleton was evaluated by laser scanning confocal microscope (LSCM).

RESULTSThe osteoblasts were inoculated after 12 h; these osteoblasts then spread along the surface of the titanium plate. In GMAO and SBMAO groups, the osteoblasts converged in the hole. The actin fibers in each group were clearly visible. In particular, the actin fibers in GMAO and SBMAO groups were arranged in parallel and formed bundles that extended into the holes.

CONCLUSIONAfter microarc oxidation, the titanium surface significantly affected the morphology and cytoskeleton of osteoblasts.

Actins ; Cytoskeleton ; Humans ; Microscopy, Electron, Scanning ; Microtubules ; Osteoblasts ; Oxidation-Reduction ; Surface Properties ; Titanium