1.To Extend the Function of Role Models of Advanced Individuals among College Students——Enlightenment of the Book "55 Proposals for a Successful College Life"on the Moral and Political Instruction for College Students
Chinese Medical Ethics 1995;0(03):-
This paper analyzes shortcomings and insufficiencies in the moral and political instruction for college students,introduces good examples and novel concepts in the book of "55 Proposals for a Successful College Life",and points out a series of enlightenment for the improvement of the moral and political instruction by extending the function of role models of advanced individuals among college students,adapting multiple forms of instruction,stressing the combination of cross-subjects,and making the instruction content and forms closer to students' realistic conditions by.
2. Morphology of A1 segment of anterior cerebral artery and anterior communicating artery aneurysms
International Journal of Cerebrovascular Diseases 2019;27(11):877-880
Anterior communicating artery aneurysms (ACoAA) accounts for about 30% of all intracranial aneurysms, and their occurrence and morphology are closely related to A1 segment of anterior cerebral artery. The dominant blood supply of A1 segment of unilateral anterior cerebral artery and the angle between A1 segment and A2 segment are important factors for the formation and development of ACoAA. The aneurysmal orientation of ACoAA is closely related to the morphological features of the A1 segment of the dominant anterior cerebral artery. The morphology of the A1 segment of anterior cerebral artery has an important effect on treatment and outcome of ACoAA.
3.Protective effect of Epothilone D against traumatic optic nerve injury in rats.
Peng Fei WANG ; Sheng Ping LUO ; Chen SHEN ; Zhe Hao YU ; Zu Qing NIE ; Zhi Wei LI ; Jie WEN ; Meng LI ; Xia CAO
Journal of Southern Medical University 2022;42(4):575-583
OBJECTIVE:
To investigate the therapeutic effect of Epothilone D on traumatic optic neuropathy (TON) in rats.
METHODS:
Forty-two SD rats were randomized to receive intraperitoneal injection of 1.0 mg/kg Epothilone D or DMSO (control) every 3 days until day 28, and rat models of TON were established on the second day after the first administration. On days 3, 7, and 28, examination of flash visual evoked potentials (FVEP), immunofluorescence staining and Western blotting were performed to examine the visual pathway features, number of retinal ganglion cells (RGCs), GAP43 expression level in damaged axons, and changes of Tau and pTau-396/404 in the retina and optic nerve.
RESULTS:
In Epothilone D treatment group, RGC loss rate was significantly decreased by 19.12% (P=0.032) on day 3 and by 22.67% (P=0.042) on day 28 as compared with the rats in the control group, but FVEP examination failed to show physiological improvement in the visual pathway on day 28 in terms of the relative latency of N2 wave (P=0.236) and relative amplitude attenuation of P2-N2 wave (P=0.441). The total Tau content in the retina of the treatment group was significantly increased compared with that in the control group on day 3 (P < 0.001), showing a consistent change with ptau-396/404 level. In the optic nerve axons, the total Tau level in the treatment group was significantly lower than that in the control group on day 7 (P=0.002), but the changes of the total Tau and pTau-396/404 level did not show an obvious correlation. Epothilone D induced persistent expression of GAP43 in the damaged axons, detectable even on day 28 of the experiment.
CONCLUSION
Epothilone D treatment can protect against TON in rats by promoting the survival of injured RGCs, enhancing Tau content in the surviving RGCs, reducing Tau accumulation in injured axons, and stimulating sustained regeneration of axons.
Animals
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Disease Models, Animal
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Epothilones
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Evoked Potentials, Visual
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Nerve Regeneration/physiology*
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Optic Nerve Injuries/metabolism*
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Rats
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Rats, Sprague-Dawley
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Retinal Ganglion Cells/physiology*
4.Effects of cannabidiol on the expression of Occludin and ZO 1 and the permeability of blood brain barrier in the cerebral cortex of rats with traumatic brain injury
Jiali Li ; Yan Cao ; Tenghan Ling ; Aiping Yin ; Hengxi Li ; Jinghui Li ; Ruilin Zhang ; Haiying Wu ; Ping Li
Acta Universitatis Medicinalis Anhui 2024;59(3):478-483
Objective :
To ob serve the expression and trends of tight j unction proteins Occludin and zonula occluden-1 (ZO-1) in blood brain barrier (BBB) of rats with traumatic brain injury (TBI) , and to explore the intervention effect of cannabidiol (CBD) on the BBB .
Methods :
The TBI model of rat was prepared by modified “Feeney free fall method ”and randomly divided into three groups : the sham operated group ( Sham group) , the model group (TBI + vehicle group) and the CBD intervention group ( TBI + CBD group) , with 24 rats in each group . Each group was subdivided into six time points : 8 h , 1 , 2 , 3 , 5 and 7 d after injury. The expression of Occludin and ZO-1 , which are closely related to the permeability of BBB , was detected by immunohistochemistry , immuno fluorescence staining and Western blot at different points . The permeability of BBB was detected by sodium fluores cein assay.
Results :
The results of immunohistochemistry showed that compared with Sham group , the positive ex pression of Occludin and ZO-1 decreased with time after brain trauma ( P < 0.05) , and both reached the lowest level at 2 d . The expression levels of Occludin and ZO-1 were up regulated after 1 d of CBD intervention ( P < 0.05) . Immunofluorescence staining showed a similar trend to Western blot results , with Occludin and ZO-1 fluo rescence expression intensity and protein expression reduced after TBI compared with Sham group ( P < 0.05 ) . And the expression levels of Occludin and ZO-1 were up regulated after 2 d of CBD intervention (P < 0.05) . The results of fluorescein sodium experiment showed that the BBB integrity of brain tissue was destroyed after TBI , and the permeability increased after TBI (P < 0.01) . The permeability of BBB decreased after CBD intervention (P < 0.05) .
Conclusion
The expression of tight junction proteins O ccludin and ZO-1 decreases after TBI , and the permeability of BBB is disrupted , and CBD intervention reverses the disruption of the BBB by TBI .
5.Cone-beam computed tomography-synthesized cephalograms for evaluating the vertical dimension of occlusions.
Ya-Nan WEI ; Zhi-Yun CAO ; Gang LI ; Xun SHENG ; Yi-Qing XIONG ; Wei LI
West China Journal of Stomatology 2019;37(1):53-57
OBJECTIVE:
The accuracy of the occlusion vertical dimensions of edentulous Han patients from Yunnan province was compared and analyzed on the basis of cone-beam computed tomography (CBCT)-synthesized cephalograms, closest speaking space method, and interocclusal distance.
METHODS:
A database correlating the CBCT head lateral images of Han patients from Yunnan province with normal occlusal conditions was first constructed. Then, five edentulous Han patients aged 63-78 years old from Yunnan Province were selected. NNT.View software was used to measure and analyze hard tissue cephalometric radiographs that had been transformed by the CBCT marker. The radiographs were then combined with the normal population database for the assessment of occlusion vertical dimensions. The occlusion vertical dimensions determined on the basis of CBCT-synthesized cephalograms, the closest speaking space method, and the free-way space were analyzed.
RESULTS:
The closest speaking space method was used as the standard control group, the differences between seven methods and the closest speaking space method were analyzed. The seven methods include free-way space method and six CBCT-synthesized cephalograms methods (N-ANS/ANS-Me, S-Go/N-Me, ANS-Gn/N-ANS, ANS-FH/Me-FH, ANS-Xi-Pm, and CA/LA). The seven methods were highly consistent with the closest speaking space method (intraclass correlation coefficient>0.986). The absolute values of the differences between the methods of free-way space, N-ANS/ANS-Me, S-Go/N-Me and the closest speaking space method were lower than those of the other four groups (P<0.05), while the differences between ANS-FH/Me-FH and the closest speaking space method was higher than those other groups (P<0.05).
CONCLUSIONS
CBCT-synthesized cephalograms, with the exception of ANS-FH/Me-FH, can provide references for the clinical evaluation of the occlusion vertical dimensions of patients with edentulous jaws.
Aged
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Cephalometry
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China
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Cone-Beam Computed Tomography
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Humans
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Imaging, Three-Dimensional
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Middle Aged
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Mouth, Edentulous
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Vertical Dimension
6.Effects of FasL inhibitors on corneal apoptosis and Treg after corneal transplantation in rats
Qian CAO ; Lan LI ; Yong LI ; Yun-Chuan LI ; Ying ZOU ; Jun-Jun LONG ; Liu-Yu HE ; Hao-Wen LIU
International Eye Science 2023;23(10):1621-1626
AIM:To explore the effect of intravitreal injection FasL inhibitors on corneal apoptosis, Fas, FasL expression, Treg numbers in blood and lymph nodes and rejection index in rats after corneal transplantation.METHODS:A total of 24 SD rats(24 eyes)who received penetrating keratoplasty were randomly divided into two groups: PBS group received intravitreal injection of PBS(12 rats, 12 eyes)and FasL inhibitor group(12 rats, 12 eyes). Rejection index was recorded every week and blood samples and lymph node were collected at 1, 3 and 5wk after surgery to analyze the proportions of Treg. Corneal tissue was collected for detecting the expression of Fas and FasL and number of apoptosis.RESULTS: The expression of Fas, FasL in FasL inhibitor group decreased significantly compared with the PBS group(all P<0.05); Corneal cell apoptosis significantly decreased in FasL inhibitor group, and it was the lowest at 5wk after surgery; Treg numbers in blood and lymph nodes significantly increased in FasL inhibitor group at 3wk after surgery(all P<0.05); rejection index of corneal transplantation in the FasL inhibitor group was significantly lower than that of PBS group(all P<0.05).CONCLUSION:Intravitreal injection of FasL inhibitors after corneal transplantation could reduce the apoptosis in all layers of cornea, increase the number of Tregs in blood and lymph nodes, and alleviate rejection.
7.Cannabidiol inhibits the express of apoptosis-related proteins in the testis of rats with traumatic brain injury
Jiali Li ; Tenghan Ling ; Yan Cao ; Aiping Yin ; Hengxi Li ; Li Yang ; Haiying Wu ; Ping Li
Acta Universitatis Medicinalis Anhui 2024;59(7):1128-1133
Objective :
To investigate testicular damage in traumatic brain injury (TBI) rats and to analyze the in- terventional effects of cannabidiol ( CBD) on TBI-induced testicular damage.
Methods :
18 Sprague Dawley ( SD) rats were randomly divided into three groups : the sham operation group ( Sham group) ,model group (TBI group) and treatment group (TBI + CBD group) .HE staining was used to observe the testicular histopathological changes in the rat testis.ELISA was used to detect testosterone level in rat serum.TUNEL staining was utilized to observe apoptosis,while immunofluorescence staining,Western blot and RT-qPCR were employed to evaluate Bax,Bcl-2, Cleaved Caspase-3 and TNF-α protein and mRNA expression.
Results :
HE staining showed pathological changes in the testes of TBI rats compared with those in the Sham group.ELISA assay showed a decrease in testosterone levels in the TBI group compared to the Sham group,but there was no significant difference (P>0. 05) .Immunofluores- cence results showed that the intensity of Bax fluorescence expression increased in the TBI group compared with the Sham group (P<0. 01) ,whereas the intensity of Bax fluorescence decreased and the intensity of Bcl-2 fluorescence increased in the rats after the CBD intervention (P<0. 01) .Western Blot results showed that CBD treatment in rats decreased the protein levels of testicular apoptosis-related proteins (Bax and Cleaved Caspase-3,all P<0. 05) and inflammation-related proteins ( TNF-α , P <0. 01 ) ,and increased the protein level of the anti-apoptotic protein, Bcl-2 (P<0. 05) .The trend of RT-qPCR results was similar,with mRNA expression of Bax(P<0. 05) and TNF-α (P<0. 01 ) decreased and mRNA expression of Bcl-2 increased after CBD intervention compared with the TBI group (P<0. 05) .
Conclusion
TBI induces testis injury,and CBD treatment effectively repairs apoptosis and in- flammatory in testicular tissue of TBI rats.
8. Rehabilitation intervention modes for children with autism spectrum disorder
Chinese Journal of Practical Pediatrics 2019;34(08):652-665
fication of intervention modes,and introduce some methods and their progress in China based on the evidence-based practice(EBP)and mixing methodologies.
9.Research status on the determination of occlusal vertical dimension of edentulous jaws
WEI Yanan ; SHENG Xun ; CAO Zhiyun
Journal of Prevention and Treatment for Stomatological Diseases 2017;25(12):814-816
It is the key step of the occlusal reconstruction to determine the correct occlusal vertical dimension for edentulous patients. Although there are several methods in clinical practice, there is no ideal way. This paper summarizes the following methods: inter-occlusal distance, closest speaking space method, pre-extraction record, face proportion division method and facial appearance observation, cephalometric analysis, electromyography, swallowing, bite force determination and self feeling method. In this paper, the current research status and the feasibility of each method are discussed.
10.Comparative study on the shade matching performance of dental students under D55 and D65 light sources conditions.
Xiong YIQING ; Li GANG ; Lin YUNHONG ; Cao ZHIYUN ; Wei YANAN ; Li WEI
West China Journal of Stomatology 2018;36(1):66-70
OBJECTIVE:
This study aimed to compare the effect of D55 and D65 light sources on the visual colorimetry performance of dental students by using a homemade light-source shelf.
METHODS:
Two Vitapan 3D-Master shade guides were randomly selected. One set was used as shade guides. Ten commonly used shade tabs of 2L2.5, 2M2, 2R2.5, 3M2, 3R2.5, 3L1.5, 3R1.5, 3L2.5, 4R1.5, and 4L1.5 were selected from the other set with covered value marks and numbered from 1 to 10. After the colorimetric training, 49 undergraduate dental students were randomly divided into two groups. Each student randomly selected two of the 10 shade tabs, and the colors were subsequently matched under D65 and D55 light sources from a distance of approximately 40 cm. The average color difference (ΔE) between the color selected by each participant and the actual color of shade tabs was calculated. Paired t test was used for statistical analysis.
RESULTS:
The ΔE values between the color selected by each participant and the actual color of the shade tabs under the D55 light source varied from 0 to 6.540. The average value was 2.501. The ΔE values between the color selected by each participant and the actual color of the shade tabs under the D65 light source varied from 0 to 6.610. The average value was 2.530. No statistically significant difference was observed between the results under the two light sources (P=0.921).
CONCLUSIONS
Both D55 and D65 daylight lamps can be used for daily dental colorimetry. These two different color temperatures showed no significant difference.
Color
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Colorimetry
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Dental Prosthesis Design
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Humans
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Prosthesis Coloring
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Students, Dental