In this article,the writer analyzed the current situation and problem of novelty research of traditional Chinese medicine.The main body of this article is complied with how to apply knowledge management in novelty research of traditional Chinese medicine.

The novelty research is very important for the new drug declaration. This article points out the methods on how to fill in the application, how to grasp the research content correctly and how to formulate retrieval methods in the process of novelty research.

The Document Retrieval Center of TCM, founded in 1992, plays an important role in the novelty retrieval of TCM scientific researching projects and clinic researches. But there are still some problems needed to be improved. The author suggests that relative governmental agents and staffs who engage in novelty retrieval should think highly of the novelty retieval of TCM to establish and consummate relative rules and regulations in order to provide the qualified services.

Objective To evaluate the role of different kinds of nitric oxide synthase(NOS)in the growth and angiogenesis of bladder transitional cell carcinoma. Methods Bladder transitional cell carcinoma tissue specimens were procured from 25 patients undergoing cystectomy, and compared with 6 normal bladder tissue specimens from donors in renal transplantation. Immunohistochemical staining was performed to detect the expression of 3 kinds of NOS. Results The malignant epithelial cells showed strong positive on immunohistochemistry whereas normal bladder showed only a weak positive iNOS immunostaining. The endothelial cells of precapillary vessels in the stroma of carcinoma showed a highly positive endothelial NOS (eNOS) immunostaining as compared with the stroma of normal bladder tissue. The expression of neuronal NOS(nNOS) was found in fibers in the fibromuscular stroma of the two groups. Conclusions Bladder transitional carcinoma tissue had a higher content of iNOS and which might be related to the genesis and development of bladder carcinoma.

This report investigated the development of technological searching-novelty, discussed the issues existing in technological searching-novelty, proposed the pattern and methods for constructing converge platform of TCM data searching-novelty, and finally emphasized its importance.

Objective To systematically review the efficacy and safety of mini-percutaneous nephrolithotomy (MPCNL) and flexible ureteroscopic lithotripsy (FURL) in management of lower pole kidney stones.Methods PubMed, Embase, Scopus, Cochrane library, CNKI, VIP and Wanfang Data were searched from the beginning of database to September 2016 and clinical trials that compared the two above operation for treatment of lower pole stones were collected.Two researchers independently filtered literature,extracted data and evaluated the methodological quality of research papers.The meta-analysis was performed using the RevMan 5.3 software.Results One randomized and seven non-randomized studies were analyzed, which consists of 621 patients including MPCNL group 327 cases, FURL group 294 cases.The results of meta-analysis showed that MPCNL was better than FURL in stones clearance (OR =2.65,95％ CI 1.58-4.46,P ＜ 0.01) and operative time (WMD =-21.86,95％ CI-28.52--15.20, P ＜ 0.01).FURL was better in hospital stay time (WMD =2.28,95％ CI 0.29-4.28, P =0.02), decrease in haemoglobin levels (WMD =0.78,95 ％ CI 0.68-0.89, P ＜ 0.01), bleeding (OR =5.11,95 ％ CI 1.12-23.31,P =0.04), transfusion(OR =7.04,95％ CI 1.59-31.15, P =0.01).There was no significant difference in fever,urinary tract infection, hematuria (P ＞ 0.05).Conclusions Both MPCN and FURL are safe and effective for the treatment of lower pole stones, MPCNL can get higher stone clearance rates and shorter operation time.However, FURL can get shorter hospitalization time and lower complication rates.

Objective To evaluate the efficacy and safety of dorsal onlay buccal mucosa urethro-plasty for the treatment of long anterior urethral stricture. Methods From October 2005 to SeDtem-ber 2007,57 patients(17-52 years old)underwent buccal mucosal dorsal onlay urethroplastv for the treatment of anterior urethral stricture.The mean urethral stricture length was 3.0cm(2.5-7.0 cm).Previously,29(51%)patients had experienced one direct vision internal urethrotomy(DVIU),20(35%)patients had accepted twice DVIU and 8(14%)patient had 3 times DVIU for the treatment of urethral stricture.All patients recurred urethral stricture after DVIU.The length of urethral stric-ture was measured by combined retrograde urethrography and voiding cystourethrography before ure-throplasty.All patients accepted urethroplasty at more than 6 months after last failed surgery.Buccal mucosa was harvested from right cheek.The urethra was exposed and dors'al side was rotated to out-side. A longitudinal incision was made on urethra. Buccal mucosal free grafts were sutured to the open edges of urethra with 6-0 absorbable suture. 1 8 F silicon fenestrated urethral catheter was Dlaced and kept for 4 weeks. Urethrography was used at 4 weeks and 3 months after the urethroplastv,and thenevery 6 months to evaluate the urethral recovery.Dysuria and urethral caliber less than 1 6 F which was confirmed by urethrography and urethroscopy were regarded as stricture recurrence. Resuits The mean fellow-up time were 11.2 months(1-23 months).Fifty-four(95%) patients remained stricture free.Three(5%)patients recurred at the 2 to 3 months after the operation and were treated with DVIU.Three patients had wound infection and recovered without other complication.There was no fistula,diverticulum in all patients.The pain of cheek lasted for 2 5 days(average 2.3 days).No case had hypoesthesia of cheek,obstruction of stenseffs duct or cheek scar.Conclusions Dorsal on-1ay buccal mucosal urethroplasty for long anterior urethral stricture provides stable results with few and acceptable complications.

Objective To induce the formation of hair follicles in vivo and in vitro with primary human hair follicle outer root sheath cells (ORSCs),human hair dermal papilla cells (DPCs) and fibroblasts seeded on three-dimensional alginate scaffolds at different sequences.Methods Second-passage ORSCs,DPCs and mitomycin-c-treated human fibroblasts were seeded into three-dimensional alginate scaffolds at different sequences and a certain ratio to reconstruct hair follicles in vitro.After 8 weeks of culture,some of the substitutes were subjected to hematoxylin and eosin (HE) staining,and some were transplanted into the subcutaneous tissue of bal/bcl nude mice followed by additional culture for 8 weeks.Then,the mice were sarcrificed and transplants were harvested and subjected to HE staining,immunohistochemical staining and transmission electron microscopy for the observation of hair follicle formation.Results After 8 weeks of culture in vitro,hair follicle cells were evenly scattered in three-dimensional scaffolds,with no keratinized material or hair follicle formation.When the substitutes were cultured in vivo for 8 weeks,DPCs and ORSCs gathered together to form cell aggregates,with the visualization of hair follicle-like structure in circular arrangement,which was positive for cytokeratin (CK) 14,CK15,β1-integrin and vimentin.Electron microscopy showed hair follicle cells and erythrocytes adhering to the scaffolds.One-week preculture of mitomycin-c-trcatcd human fibroblasts in the scaffolds followed by the seeding of the suspension of ORSCs and DPCs at a ratio of 1∶5 resulted in increased follicular like structures with more typical appearance in mice compared with the other strategies for cell inoculation and culture.Conclusions In the reconstructed hair follicle models,DPCs keep the capability to induce the formation of hair follicles,and ORSCs keep the characteristics of hair follicle stem cells.The optimal cell density,ratio and process were determined in the experiment,which may lay a methodological and theoretical basis for hair follicle reconstruction.

This paper expounds the needs analysis and system design of traditional Chinese medical science and technology of sci-tech novelty search management system,discusses the architecture of the system and each functional module,and summarizes and discusses the meaning of this system when applied in sci-tech novelty search.

Objective To study the effect of euthyrox on blood lipids in patients with hypothyroidism throngh the meta analysis.Methods Patients with clinical hypothyroidism theated by euthyrox on blood lipid effect of randomized controlled trials were retried by Pubmed(2000/2014-07),EMbase (2000/2014-07),the Cochrane library(2014,7),the Chinese biomedical literature database(2000/2014-07),retrieved the time limit from January 2000 to July 2014.The paper were objective evaluated of the quality,Meta analysis with Rev Man 5.3 software system applicated by Cochrane collaboration.Results Euthyrox could significantly reduce cholesterol levels of hypothyroidism patients ,it was statistically significant difference(OR=0.63,95%CI:0.71 ~0.55,P<0.001);Euthyrox could significantly reduce triglyceride levels of hypothyroidism patients,difference was statistically significant(OR =0.29,95%CI:0.34 ~0.25,P <0.001 );Euthyrox could significantly reduce low-density lipoprotein cholesterol levels of hypothyroidism patients,it was statistically significant difference(OR=0.22,95%CI:0.30 ~0.14,P<0.001);euthyrox could obviously increase high-density lipoprotein cholesterol levels, it was statistically significant difference (OR =0.21, 95% CI:0.17 ~0.26, P <0.001 ). Conclusion Euthyrox could significantly reduce total cholesterol,triglyceride,low-density lipoprotein cholesterol(hdl-c)of hypothyroidism patients,and promote the synthesis of high-density lipoprotein cholesterol(hdl-c),could effectively promote the lipid metabolism,reduce blood fat level,prevent the happening of the disease of heart head blood-vessel hypothyroidism patients,have the guide meaning to clinical treatment.