0.05).There were more patients with complete SCI and spasticity in group A(P

ObjectiveTo study microRNA (miRNA) expression and role of cell cycle regulation in decidualized endometrial stormal cells (ESC) in vitro.MethodsESC was induced decasualization in vitro and matched with non-decidualized cells as controls.The expression repertoire of miRNA was measured by microarray chip and was validated by real-time PCR.Flow cytometry was used to identify ESC cycle during decidual reaction in vitro and after miRNA222 inhibitor was transfected into it.Results (1) Between decidualized and undecidualized stromal cells,there were 49 miRNAs significantly different expression by microarray chip,including 16 miRNA up-regulation and 33 miRNA down-regulation.hsa-miR-27b,30c,143,101,181 b,29b,30d,507,23 a,222,221 exhibited significantly differential expression between decicualized and undecidualized stromal cells by real-time PCR (P ＜0.05).(2) After miRNA222 inhibitor (NC-FAM) transfection to decidual ESC,ESC were cultured by FBS medium for 24 hours,the rate of transfection was 70％.ESC were transfected with miRNA 222 inhibitor and cultured for 48 hours,the percentage of ESC at Sphase of (6.2 ± 0.7 ) ％ were significantly lower than ( 10.9 ± 0.8 ) ％ in control group ( P ＜ 0.05 ) ; the percentage of ESC at G0/G1 phase increased at transfection group [ (77.5 ± 1.3 ) ％ vs.(73.0 ± 1.6) ％ at control group ],but there was no significant difference (P ＞ 0.05 ).Decasualization ESC were transfected with miRNA 222 inhibitor and cultured for 48 h,the percentage of ESC at S-phase was ( 3.3 ± 0.6) ％ in transfection group,which were significantly lower than (7.8 ± 0.9 ) ％ in control group ( P ＜ 0.05 ).The percentage of ESC at G0/G1 phase was ( 80.7 ± 1.6 ) ％ in transfection group and ( 74.9 ± 1.1 ) ％.In control group,which did not reached statistical difference ( P ＞ 0.05).ConclusionmiRNA was involved in ESC decidual process in vitro by regulating cell cycle.

Objective:To observe the thrombolytic effect of Naoxuebao in vitro and in vivo.Methods:The changes of thrombus in the carotid arterial,plasma fibrinogen (FIB),thrombin time (TT) and the diameter of blood clot were observed using Naoxuebao (three dose groups) after thrombosis.Results:With Naoxuebao,the time of blood clot was longer,the diameter of blood clot was shortened;carotid arterial thrombosis was dissolved.The contents of FIB decreased,TT was longer,and FIB and TT had negative correlation;whereas,PLG had no obious change.Conclusion:Naoxuebao had thrombolytic effect,but the effect had no relation with plasmin activating.

Objective To improve the understanding of the epidemiology,clinical features,diagnosis and treatment of Tsutsugamushi disease,and reduce the misdiagnosis rate.Methods The clinical data of 56 cases of Tsutsugamushi disease misdiagnosed were analyzed retrospectively.Results 28 cases were misdiagnosed as acute upper respiratory tract infection(50.0%),3 cases were malaria(5.4%),1 case was cholecystitis(1.8%),24 cases were infectious fever(42.8%).The average misdiagnosed time:10.3days.55 patients were found Eschar or ulcer (98.2%),54 cases were found Eschar or ulcer on one part of body,1 case was found two eschars.54 cases had been effectively treated,50 cases were treated with chloramphenicol,4 cases with azithromycin.The average time of temper-ature that dropped to normal were respectively:1.3d,1d.Conclusion awareness of Tsutsugamushi disease should be raised,history seriously should be inquired and specific signs should be identified,which can degrade the misdiagnosis rate and reduce the mortality rate.

AIM and METHODS: To study the effect of Nao-xue-bao at three different doses on blood coagulation,platelet aggregation by observing the changes in activated partial thromboplastin time (APTT), prothrombin time (PT), antithrombin-Ⅲ(AT-III), fibrinogen(Fng), plasminogen(Plg) and platelet aggregation(PAG). RESULTS: Compared with thrombosis group, the rats treated with Naoxuebao showed that the plasma APTT,PT were longer, and the activity of AT-III was increased. The content of Fng was reduced, TT was longer, there was a negative correlation between Fng and TT. Furthermore PAG-1, PAG-5 and PAG-M were reduced. CONCLUSION: Nao-xue-bao could inhibit thrombosis in different keys of blood coagulation.

AIM and METHODS:To study the effect of Nao-xue-bao at three different doses on blood coagulation，platelet aggregation by observing the changes in activated partial thromboplastin time (APTT), prothrombin time (PT), antithrombin-Ⅲ(AT-III), fibrinogen(Fng), plasminogen(Plg) and platelet aggregation(PAG). RESULTS:Compared with thrombosis group, the rats treated with Naoxuebao showed that the plasma APTT,PT were longer, and the activity of AT-III was increased. The content of Fng was reduced, TT was longer, there was a negative correlation between Fng and TT. Furthermore PAG-1, PAG-5 and PAG-M were reduced. CONCLUSION:Nao-xue-bao could inhibit thrombosis in different keys of blood coagulation.

Objective　To investigate the expression of LPS receptor-CD14(CD14) on the membrane of Kupffer′s cells (KCs) induced by Lipopolysaccharide (LPS )and its role in activation of KCs and production of cytokines. Methods　KCs were isolated by collagenase perfused Wistar rats and routinely cultured in 24-well dishes for 12 h. Cells were harvested and adjusted to a concentration of 1×106/ml/well and were devided into two groups. Group of LPS: KCs were induced with different concentration of LPS (0, 100 ng/ml, 1 μg/ml and 100 μg/ml). Group of PI-PLC: KCs were pre-incubated for 30 min with one unit of phophatidy linositol specific phospholipase C (PI-PLC) before different concentrations of LPS were added. KCs were cultured for 30 and 60 min respectively. Supernatants were then collected for measuring the level of TNFα and IL-6. Cells were stained by indirect immunofluorescent method ( rabbit anti-CD14 antibody and goat anti-rabbit IgG conjugated with FITC ) and analyzed with flow cytometer (FCM). The percentage and mean fluorescence intensity (FI) of CD14-positive cells were taken as the indexes. Results　In LPS group, after incubation of cells with increasing concentration of LPS, a significant increase in the percentage of CD14 positive KCs were found and the mean FI was stronger when compared with the control points or the group of PI-PLC. The levels of TNFα and IL-6 in supernatant also increased (P<0.01). In group of PI-PLC, decreased percentage of CD14 positive KCs and weakened mean FI were found when compared with group of LPS. The increasing production of TNFα and IL-6 slowed down in the group of PI-PLC. Conclusion　CD14 expression of KCs might be up-regulated by LPS with increase of some cytokines. The production of cytokines in KCs induced by LPS is partially inhibited by PI-PLC.

Objective To evaluate the curative effects of carbon nanoparticles on central lymphnode dis -section in papillary thyroid carcinoma ( PTC) .Methods 72 PTC patients were randomly divided into two groups:carbon nanoparticle group(n=32)and the control group(n=32).Patients in the carbon nanoparticle group were injected with carbon nanoparticles during surgery .Patients in the control group had conventional surgery .The number of lymph nodes being dissected ,lymph node metastasis , and the rate of hypoparathyroidism were compared between the two groups .Results The number of lymph nodes dissected in nanoparticle group ( n =312 ) was much bigger than that in the control group (n=189)(P<0.01;t=8.476).The incidence of hypoparathyroidism in nanoparticle group(n=1)was much lower than that in the control group (n=8)(P<0.05;χ2 =4.571).The metastasis of lymph nodes has no significant difference between the two groups (P>0.01;χ2 =1.048).Conclu-sions The lymphatic tracer technique may improve the number of lymph nodes dissected in central region of PTC and reduce parathyroid gland damage .

Objective To study the expression of signal transducer and activator of transcription 3 (STAT3) and Suppressor of cytokine signaling 3(SOCS3) in the middle ear cholesteatoma epithelium ,and the possible roles of STAT3 and SOCS3 in middle ear cholesteatoma .Methods The immunohistochemical assay was used to detect ex-pression of STAT3 and SOCS3 protein in 30 cases of middle ear cholesteatoma epithelial tissue and 20 cases of nor-mal external auditory canal skin tissues as the control group .Results STAT3 immunoreactivity was detected in the nuclei and cytoplasm of epithelial cells .The expression rates of STAT3 in middle ear cholesteatoma epithelial tissue were 76 .7% and higher than in the normal epithelium (25 .0% ) .The differences between the two groups were sta-tistically significant (P<0 .05) .SOCS3 immunoreactivity was detected in the cytoplasm of epithelial cells .The ex-pression rates of SOCS3 in middle ear cholesteatoma epithelial tissue were 33 .3% and lower than in the normal epi-thelium (65 .0% ) .The differences were statistically significant (P<0 .05) .The expression of STAT3 and SOCS3 in the middle ear cholesteatoma had negative correlation (r= - 0 .476 ,P<0 .05) .Conclusion The abnormal ex-pression of STAT3 and SOCS3 in the middle ear cholesteatoma may be involved in hyper proliferation and anti -ap-optosis of cholesteatoma cell ,and play an important role in the formation and development of middle ear cholesteatoma .

Objective To investigate the related factors of postoperative hypocalcemia after thyroid carc-er surgery.Methods 346 cases of thyroid carcer patients undergoing surgery from Jan .2013 to Dec.2013 in the First Affiliated Hospital of Zhengzhou University were retrospectively analyzed .Results 129 cases developed hepocalcemia after thyroid surgery .Among the related factors that may cause postoperative hypocalcemia , the scope of surgery , parathyroid injury and secondary surgery might play important roles .Conclusions The inci-dence of postoperative hypocalcaemia is high after total thyroidectomy .Patients with reoperation and lymph node dissection have an increased rate of postoperative hypocalcaemia .In order to reduce hypocalcaemia incidence ,sur-gons need to protect parathyroid blood supply in thyroid surgery and give calcium after surgery .