1.Research on chemical reactions during ginseng processing.
Miao ZHANG ; Kun-Ming QIN ; Wei-Dong LI ; Fang-Zhou YIN ; Hao CAI ; Bao-Chang CAI
China Journal of Chinese Materia Medica 2014;39(19):3701-3706
As a kind of commonly used traditional Chinese medicine, ginseng has a high reputation at home and abroad. The research of ginseng has been expanded to medicine, pharmacy, biology, food science and other fields, with great achievements in recent years. Ginseng contains ginsenosides, volatile oil, carbohydrates, amino acids, polypeptides, inorganic elements and othser chemical constituents. Each component has extensive physiological activity, and is the base of ginseng's effect. After processing, the complicated changes are taken place in the constituents of ginseng, and some new substances produced. This paper aims to review the studies on chemical constituents and their mechanisms during ginseng processing, and the ideas, methods and the direction of the development of traditional Chinese medicine processing in the future.
Chemistry, Pharmaceutical
;
methods
;
Drugs, Chinese Herbal
;
chemistry
;
Panax
;
chemistry
;
Plants, Medicinal
;
chemistry
2.Changes of IL-12、 IFN-? and IL-4 in peripheral blood of postoperative gastric cancer patients vaccined with dendritic cells
Hao WANG ; Peiwu YU ; Qun HAO ; Kun ZHANG ; Yuehe WANG ; Yan SHI ; Zhimin CAI
Chinese Journal of General Surgery 2000;0(11):-
Objective To investigate effects of dendritic cells (DC) vaccine on immune function in postoperative gastric cancer patients Methods DCs were extracted and pulsed with self tumor antigens in vitro Fifty patients with gastric cancer were divided into 2 groups randomly Four times of DC vaccinations were administered at 7 days intervals to the postoperative patients in treatment group after they underwent chemotherapy The control group were treated with chemotherapy only The level of IL 12 、 IFN ? and IL 4 in peripheral blood were analysed Results In DCs group, the levels of IL 12 were 37?4、 68?6、 96?12 and 59?9 pg/ml, the levels of IFN ? were 61?12、 134?19、 145?20 and 111?15 pg/ml, and levels of IL 4 were 55?7、 49?6、 46?5 and 50?8 pg/ml before and 2, 4 and 8 weeks after vaccination, respectively In control group, the levels of IL 12 were 39?7、 45?9、 44?10 and 44?6 pg/ml, the levels of IFN ? were 63?10、 61?13、 62?11、 61?7 pg/ml, and levels of IL 4 were 52?11、 55?9、 53?10、 55?8 pg/ml before and 2, 4 and 8 weeks after vaccination, respectively The levels of IL 12 and IFN ? increased significantly in DCs group No severe side effects were found during treatment Conclusion DC vaccine improves immune function of posroprative patients with gastric cancer
4.Research status and prospect of primary processing of traditional Chinese medicinal materials.
Lin-wei CHEN ; Kun-ming QIN ; Yan-hui ZHU ; Hao CAI ; Wei-dong LI ; Bao-chang CAI
China Journal of Chinese Materia Medica 2015;40(4):602-606
The primary processing is important links and closely related to the quality of traditional Chinese medicinal materials, and is not only cleaning of remove the non-officinal parts, drying for termination the physiological status of organisms, but also retaining the most active substances, decreasing the toxic components, and promoting the transformation among chemical ingredients through primary processing. So the traditional primary processing endows with characters, quality, specifications and properties of traditional Chinese medicine, and embodies some important science truth. The traditional primary processing method and technology systems are derived from the long-term practices and experiences, which are distinctive, colorful, diverse, and scientific, which are helpful to development and utilization of traditional Chinese medicine resources. This paper systemically expounds the research status of the Chinese medicine processing method, summarizes the problems in the primary processing of traditional Chinese medicinal materials research, and prospects its bright future.
Chemistry, Pharmaceutical
;
methods
;
trends
;
Drugs, Chinese Herbal
;
chemistry
;
pharmacology
;
Medicine, Chinese Traditional
5.Prenatal diagnosis of prelingual deafness by determination of SLC26A4 gene mutation
Hao HU ; Lingqian WU ; Desheng LIANG ; Yong FENG ; Fang CAI ; Kun XIA ; Qian PAN ; Zhigao LONG ; Heping DAI ; Jiahui XIA
Chinese Journal of Obstetrics and Gynecology 2000;0(09):-
G mutation of SLC26A4, the parents and the second child were carriers of the same mutation, while the fetus had a wild-type form. Conclusion It is feasible to identify deafness related genes by screening for GJB2 and SLC26A4 mutation, thus providing correct prenatal diagnosis and avoiding deaf delivery of baby.
6.Progress in research of processing of fructus Evodiae-Rhizoma Coptidis and research thoughts and methods of its processing mechanism.
Xiaobin JIA ; Jun JIANG ; Bin CHEN ; Yan CHEN ; Kun HAO ; Baochang CAI ; Hao WU ; Guangming YANG
China Journal of Chinese Materia Medica 2009;34(10):1314-1317
This article ascended the history and successive changes of the processing research of Fructus Evodiae-Rhizoma Coptidis, and its application in classic prescription; made a summary on the research of the processing technology and substances of Fructus Evodiae-Rhizoma Coptidis; discussed the progress in the research of processing of Fructus Evodiae-Rhizoma Coptidis; expounded the overview on mechanism research of the processing of Fructus Evodiae-Rhizoma Coptidis, and proposed new thoughts and methods for the processing of Fructus Evodiae-Rhizoma Coptidis.
Chromatography, High Pressure Liquid
;
Coptis
;
chemistry
;
Drug Screening Assays, Antitumor
;
Drugs, Chinese Herbal
;
Evodia
;
chemistry
;
Free Radical Scavengers
;
Phytotherapy
;
Plant Extracts
;
therapeutic use
;
Prescriptions
;
Research
;
Technology, Pharmaceutical
7.Observation on therapeutic effect of acupoint application of Chinese medicine on bronchial asthma.
Wei-Hua CHEN ; Kun XIN ; Chen-An CAI ; Feng HAO ; Yong CAO
Chinese Acupuncture & Moxibustion 2009;29(4):272-274
OBJECTIVETo compare therapeutic effects of acupoint application of Chinese medicine and routine acupuncture on bronchial asthma.
METHODSOne hundred cases were randomly divided into an observation group and a control group, 50 cases in each group. The observation group was treated with application of Chinese medicine at Dazhui (GV 14), Feishu (BL 13), Gaohuang (BL 43), Danzhong (CV 17), Qihai (CV 6) in the dog days; and the control group was treated with acupuncture at Zhongfu (LU 1), Tiantu (CV 22), Danzhong (CV 17), Dingchuan (EX-B 1), Fenglong (ST 40). The long-term therapeutic effects were observed.
RESULTSThe total effective rate was 94.0% in the observation group and 76.0% in the control group with a significant difference between the two groups (P < 0.05).
CONCLUSIONThe therapeutic effect of acupoint application of Chinese medicine in the dog days on bronchial asthma is significantly superior to that of routine acupuncture.
Acupuncture Points ; Acupuncture Therapy ; methods ; Adolescent ; Adult ; Aged ; Asthma ; pathology ; therapy ; Child ; Female ; Humans ; Male ; Medicine, Chinese Traditional ; methods ; Middle Aged ; Time Factors ; Treatment Outcome ; Young Adult
8.Effect of early endovascular embolizafion using detachable coils for management of intraeranial aneurysm rupture
Kun-Hao CAI ; Li-Meng DAI ; Dong CHEN ; Hong CHEN ; Yong-Yang ZHA
Chinese Journal of Neuromedicine 2008;7(9):950-952
Objective To evaluate the therapeutic effect of endovascular embolization using Guglielmi detachable coils(GDCs)or electrolytically detachable coils(EDCs)for early treatment of ruptured intracranial aneurysms. Methods A total of 208 patients with 220 intraeranial aneurysms were treated in the emergency setting using GDCs or EDCs after aneurysm rupture. Results All the 220 aneurysms were successfully embelized with the detachable coils.Complete embolization(>95%)of the aneurysms was achieved in 128 of the 220 eases,and 80%-95%embolization in 80 cases,with the other 12 having an partial embolization less than 79%.Aneurysm rupture occurred in 2 patients during the embolization procedure,one of which was cured and the other died.Five patients sustained cerebral infarction,resulting in permanent hemiplegia or monoplegia after local thrombolytic therapy.In 3 cases,a small portion of the coil remained in the parent artery,but no complication occurred after anticoagulant therapy.Twelve patients experienced aneurysm recurrence,but Was successfully managed with a second coil embolization procedure.Conclusion GDCs and EDCs Can be safe,reliable and effective for early endovascular embolization of ruptured intracranial aneurysrns.
9.Optimization of extraction process for Baizhu Shaoyao San by multi-index orthogonal experiment.
Li-Juan ZHENG ; Kun-Ming QIN ; Hao CAI ; Gang CAO ; Bao-Chang CAI
China Journal of Chinese Materia Medica 2013;38(10):1504-1509
Baizhu Shaoyao San is a famous traditional Chinese medicine formula, which is efficient to diarrhea clinically. However, there are very few reports for extraction process of Baizhu Shaoyao San at present. In this article, an orthogonal test was conducted, with 10 major components and extractum of Baizhu Shaoyao San as indexes, and data was processed by the compound weight method, in order to detect four factors, including the concentration of ethanol, the dosage of ethanol, the soaking and extracting times, and optimize the extraction process of Baizhu Shaoyao San. The results showed that the optimal extraction process was adding 12-fold ethanol of 70% concentration, soaking for 1 hours, and extracting for twice, 2 hours each time. Among the indexes, the extracting time made significant impact. The optimized method is stable and reliable, and can provide reference for further development and utilization of the formula.
Chemical Fractionation
;
methods
;
Chromatography, High Pressure Liquid
;
Drugs, Chinese Herbal
;
analysis
;
isolation & purification
;
Plants, Medicinal
;
chemistry
10.Study on quality evaluation of cimicifugae rhizoma from different producing areas by HPLC fingerprint.
Bao-Jia SHEN ; Kun-Ming QIN ; Xing-Hai ZHANG ; Qi-Di LIU ; Hao CAI ; Xiao LIU ; Bao-Chang CAI
China Journal of Chinese Materia Medica 2013;38(13):2155-2158
To establish a fingerprint for Cimicifugae Rhizoma from different producing areas. Column kromasil (4.6 mm x 250 mm, 5 microm) was employed with acetonitrile-0.1% formic acid solution as the mobile phase for gradient elution. The flow rate was 1.0 mL x min(-1), the detection wavelength was 254 nm. Twenty chromatographic peaks were extracted as the common peaks of fingerprint, and 21 batches of samples were compared and classified with such methods as similarity evaluation, cluster analysis and principle component analysis. The results showed 12 common peaks and three categories of samples. The method was so highly reproducible, simple and reliable that it could provide basis for quality control and evaluation of Cimicifugae Rhizoma from different producing areas.
Chromatography, High Pressure Liquid
;
methods
;
Cimicifuga
;
chemistry
;
Drug Stability
;
Quality Control