In recent several decades, studies have reported the representation of attention deficit/hyperactivity disorder(ADHD) symptoms could co-morbid in children with autism spectrum disorders(ASD).Compared with the ASD-only group, children with both ADHD and ASD often have a more obvious representation of autism symptom as well as more impaired cognitive functions which could deeply impact on their daily life.There is a brief review of observation and executive functions in ASD children co-morbid ADHD-symptoms.

Objective To observe the efficacy and safety of Compound Kaliziran Tincture combined with 0.1%Mometasone Furoate Cream in the treatment of vitiligo. Methods A total of 120 patients of vitiligo were randomly recruited into a control group (54 cases) and a treatment group (66 cases). The treatment group was treated with compound kaliziran tincture combined with 0.1% mometasone furoate cream for external use, while the control group was treated with topical 0.1% mometasone furoate cream. Both groups received the treatment for one course of three months. Efficacy was assessed at the end of the course. Results The total effective rate was 60.94% and 32.69% in the treatment group and the control group respectively, the difference showing statistical significance (χ2=7.86, P＜0.05) . Conclusion It is safe and effective for compound kaliziran tincture combined with 0.1% mometasone furoate in the treatment of vitiligo with few side effects. The combined therapy is better than single using 0.1% mometasone furoate cream.

Coronary heart disease has become a major threat of human health, and its incidence rate remains surprisingly high.In recent years, technology on its diagnosis and treatment has developed greatly.The present article made a review on related new diagnosis and treatment so as to provide reference for clinical diagnosis and treatment of coronary heart disease.

Bilateral ablation of the cerebral parietal cortex in adult rats was performed. After appropriate days, the tissue surrounding the wound was removed and the brain wound tissue extract (EWTE) was prepared. Newborn rat cerebral cortical neurons were used as a culture model to test the neuronotrophic factors (NTFs) and neuritepromoting factors (NPFs) in EWTE. In order to investigate the origin of above mentioned factors whether related to the macrophages which appeared in the brain wound region at early stage, we designed to culture macrophages and collected the macrophage conditioned medium (M?CM) to measure their NTF and NPF activities for cultured cerebral cortical neurons. On the other hand, we also observed the effect of EWTE and M?CM on PC 12 (phehrmytema) cells and further studied the action of NPFs. Cur experimental results show that EWTE and M?CM contained NTFs and NPFs for cultured cerebral cortical neurons. These factors appeared in EWTE at 4 days post-lesion, with maximal level of their activities reached between 5 and 6 days post-lesion and there was another peak of NPF activity at 9 days post-lesion, until 13 days post-lesion also detected their activities. The NTF activity in M?CM was lower than that in BWTE, in contrast, the NPF activity in M?CM was higher than that in BWTE. There was NPF activity to PC 12 cells in BWTE and M?CM. According to the experimental assays, we suppose that the neurotrophic factors in BWTE mainly come from the macrophages which appear in the lesion site at early stage of injured brain, subsequently, it may relate to the astrocytes. The components of these factors may be complexity and multiplicity that remain to be solved.

Spinal giant cell tumor of bone (GCTB) is a kind of primary benign bone tumors in the spine. It is rich in blood supply,aggressive,and easily recurring and lung metastasizing. So the benign GCTBs of the spine remains a challenge to treat.This article reviews the therapeutic methods in spinal GCTBs,including surgery,radiation therapy,arterial embolization. The treatment for the tumor with lung metastasis is also covered in this review. It is established that En bloc resection with wide margins is the most effective method to spinal GCTBs. To eliminate the residual tumor cells,adjuvant radiation should be done when complete resection is not available. Arterial embolization can be used to treat the huge sacral GCTBs.For those with lung metatasis,they can be controlled by lobectomy and /or chemotherapy.

Objective:To observe the effect of ischemic preconditioning(IP)on hepatocellular apoptosis caused by ischemia-reperfus ion(IR)injury and expression of apoptosis regulatory genes(Survivin,HIF-1?)protein,to explore the protective mechanisms of IP on IR injury in rat liver.Methods:Ninty SD rats were randomly divided into sham operation(SO)group,ischemia-reperfusion(IR)group,ischemic preconditioning(IP)group.In SO group,the hepatoduodenal ligament of rat was only pulled and separated,and then closed the abdomen;IR group subjected to 30 minutes of ischemia;IP group was achieved by ischemia for 5 minutes,followed by reperfusion for 5 minutes before continuous to ischemia for 30 minutes.Ten rats of each group were killed after reperfusion for 2,6,12,24 hours respectively,then liver tissues were taken out.The rats in SO group were killed after operation for 2 hours.To detect the Apotosis index(A)Iand Survivin,HIF-1? protein levels.Results:AI of hepotocytes significantly increased in IR group and IP group than that in SO group(P

Objective To explore the effects of antagonist of angiotensin II(Ang II) receptors on acute pancreatitis(AP) of rats. Methods The Sprague-Dawley rats were divided randomly into normal group, AP group, AP+lower dose of valsartan(5mg/kg) group , AP+higher dose of valsartan(8mg/kg) group, lower dose of valsartan group and higher dose of valsartan group, and each group containing 6 animals. AP animal models were induced by intraperitoneal injection of cerulein. Valsartan was given by intragastric administration. The histopathological changes of pancreas were observed and scored, and the pancreatic edema was evaluated. The levels of plasma amylase (AMY) and pancreatic myeloperoxidase (MPO) were measured. Results Compared with AP group, the pathologic lesions of pancreas and pancreatic edema decreased in the group AP+ valsartan, and the levels of plasma AMY and MPO also reduced. Conclusion Pretreatment with antagonist of angiotensin II receptors in rat acute pancreatitis can markedly reduce pancreatic injury and inflammation.

Objective To investigate the sensitivity and specificity of different methods for the detection of mycoplasma pneu‐moniae .Methods The serum and throat swab specimens were collected from 199 pneumonia patients .ELISA and real‐time fluores‐cence quantitative polymerase chain reaction (FQ‐PCR) assays were used to detect the serum mycoplasma pneumoniae IgM antibod‐y and DNA .Results Among 199 pneumonia patients ,61 cases were diagnosed as mycoplasma pneumoniae pneumonia according to the diagnosis standard .The sensitivity and specificity of ELISA and FQ‐PCR for detecting mycoplasma pneumoniae were 86 .9%and 96 .7% ,and 78 .3% and 97 .1% ,respectively .Conclusion The sensitivity and specificity of FQ‐PCR for detecting mycoplasma pneumoniae are higher than those of ELISA .

Objective:To identify the expression of egfp-hri fusion on NIH3T3 cells which is carried by recombinant retrovirus. Methods:The egfp-hri fusion gene was integrated intoNIH3T3 cells by the infection of recombinant viral supernatant from the G418 resistent PA317 cells transfected stably with the plasmid of pLNCX-EGFP-C1-hri. The expression effect of egfp-hri fusion was visualized under the fluorescent microscope, while the expression levels of egfp-hri fusion protein by Western blot. Results:The observation from fluorescent microscope showed the green fluorescent was obviously observed in the cytoplasm , western-blotting showed the expression level of egfp-hri fusion gene increased by 83%and 81%, as compared with those uninfected cells and in the cells infected with empty vector, respectively. Conclusion:The infected NIH3T3 cells can express egfp-hri fusion protein successfully.