No abstract available.
Child* ; Humans ; Mass Screening* ; Seoul*

No abstract available.
Bone Marrow Transplantation* ; Bone Marrow*

BACKGROUND: The use of human umbilical cord blood(CB) as a source of hematopoietic stem cell transplantation for the treatment of pediatric diseases has been well established. In previous study, we reported the method of improving the recovery rate of cryopreserved CB mononuclear cells after thawing and the possibility of long-term cryopreservation. In this study we compared the recovery rate of the cryopreserved mononuclear cells from large volume of CB according to various freezing conditions and stored CB mononuclear cells as a source of transplantation. METHODS: Twenty five CB from Kyung-pook National University Hospital and the Hana Obstetric/Gynecology Hospital, Taegu, Korea were used. The mononuclear cell separation was done with Hypaque and CD34+ cells were collected by Fenwal with the monoclonal antibody and immunobeads within 6 hours after collection. The cell count and the viability rate was done by hemocytometer with trypan blue exclusion method. The mononuclear cells were cryopreserved with two different concentrations of fetal bovine serum and of dimethylsulfoxide and with two different methods of direct and programmed freezing in the liquid nitrogen. The recovery rate was compared after thawing. The differentiation ability of thawed mononuclear cell was performed with in vitro colony culture using methylcellulose medium and GM-CSF. The family history of inherited blood cell diseases, tests for viral(HIV, HBV, HCV and CMV) and bacterial (syphilis, etc) contamination and HLA and blood typing were done for the future transplantation source. RESULTS: The mean volume of collected CB was 117(49-150)ml and the mean number of mononuclear cells was 5.0(2.8-7.5) x 10(6)/ml. The viability rate was 95.3-98.6%. The recovery rate of mononuclear cells was higher in the group of medium containing 10% of fetal bovine serum and 10% of dimethylsulfoxide after 30 days programmed freezing in liquid nitrogen(-196 degrees C). The recovery rate of long-term(180 days) cryopreserved mononuclear cells was 72.8%. The colony formations in culture of mononuclear cells were not different between before-and 14 days after-freezing. The seven total volume of CB mononuclear cells which had no family history of inherited blood cell diseases, no viral and bacterial contamination were cryopreserved after HLA and blood typing for the future transplantation. CONCLUSION: In summary, because the long-term cryopreservation of CB mononuclear cells was possible with good recovery rate we expect the establishment of CB bank will overcome the limitation of donor in Korea in the near future.
Blood Cells ; Blood Grouping and Crossmatching ; Cell Count ; Cell Separation ; Cryopreservation* ; Cytomegalovirus ; Daegu ; Diatrizoate ; Dimethyl Sulfoxide ; Fetal Blood* ; Freezing ; Granulocyte-Macrophage Colony-Stimulating Factor ; Hematopoietic Stem Cell Transplantation* ; Hematopoietic Stem Cells* ; HIV ; Humans ; Korea ; Methylcellulose ; Nitrogen ; Syphilis ; Tissue Donors ; Trypan Blue ; Umbilical Cord

No abstract available.
Hemostasis* ; Thrombosis*

No abstract available.
Pre-Eclampsia* ; Pregnancy*

No abstract available.
Epilepsy*

No abstract available.
Complementary Therapies* ; Humans

There is inherent diffi culty in identifying the epileptogenic zone in nonlesional neocortical epilepsy, which leads to the incomplete resection. However, with careful interpretation of other studies including functional neuroimaging and concordant results, surgical treatment can benefi t patients with nonlesional neocortical epilepsy. Two recent large studies including ours demonstrated that seizure free outcomes were 47 and 55% for nonlesional TLE, and 41 and 43% for nonlesional extratemporal lobe epilepsy patients. Concordance with two or more presurgical evaluations among interictal EEG, ictal EEG, FDG-PET, and ictal SPECT was signifi cantly related to a seizure-free outcome. However, we should always the possibility of false localization of ictal EEG or functional neuroimaging in nonlesional neocortical epilepsy. Careful placement of intracranial electrodes on adjacent areas should be needed for these patients. The repositioning of intracranial electrodes might identify a new ictal onset zone. Consideration of one-week interval repositioning of intracranial electrodes could be helpful in selected patients. Intracranial EEG is one of the most important procedures in planning surgery and achieving a good surgical outcome in resective epilepsy surgery. Slow propagation and focal or regional ictal onset were associated with a seizure-free outcome. Resection that includes more electrodes with ictal rhythm or interictal abnormalities predicts a good surgical outcome.

No abstract available.
Superoxide Dismutase* ; Superoxides*

The purpose of this study is to observe the changes in 99mTc-MDP uptake of the tibia during blood-flow and skeletal phases and determine the clear borderline between both phases. Serial 99mTc-MDP uptake measurements were performed, as control, in ten matured rabbits with skin incision only and in thirty matured rabbits with fractures of middle and distal one-third of tibia, at ten minutes, thirty minutes, one hour, two hours and four hours after injection of bolus. A value in uptake ratio was calculated by measurements of 99mTc-MDP uptake at each observation time for analogous regions of normal and fractured tibia applied with or without tourniquet. The results obtained were as follows: 1. Group I (skin incision only in ten rabbits) 1) Without tourniquet, the mean 99mTc-MDP uptake ratio tibia was value of 1.05 at ten minutes, 0.97 at thirty minutes, 0.99 at one hour, 1.01 at two hours, 0.97 at three hours and 1.08 at four hours. 2) With tourniquet, the mean 99mTc-MDP uptake ratio of tibia was value of 0.61 at ten minutes, 0.64 at thirty minutes, 0.76 at one hour, 0.98 at two hours, 0.94 at three hours and 1.05 at four hours. 2. Group II (fracture with K-wire fixation in twenty rabbits) 1) Without tourniquet, the mean 99mTc-MDP uptake of tibia was value of 0.73 at ten minutes and 0.80 at two hours. 2) With tourniquet, the mean 99mTc-MDP uptake ratio of tibia was value of 0.45 at ten minutes and 0.76 at two hours. 3) There were no significant change of the 99mTc-MDP uptake ratio in two to four hours after fracture whether the tourniquet was applied on or not. 3. In cases with tourniquet application, there were no significant changes of 99mTc-MDP uptake ratio during two to four hours in group I anda II. This experiments suggest the optimal time for evaluation of the skeletal phase is in two hours following the injection of bolus (99mTc-MDP).
Rabbits ; Skin ; Technetium Tc 99m Medronate ; Tibia ; Tourniquets