Objective To explore the application of association rules with Constraints in medical data analysis.Methods Constraints were applied in mining association rules of medical data by analyzing the characteristics of the data.An effective algorithm for mining association rules with constraints from medical data called CAMD was developed.The algorithm was applied to the analysis ofcoronary heart disease dataset.Results Assigning minimum support and minimum confidence as 0.20 and 0.60,respectively,with data from 303 cases of coronary heart dieses analyzed,8 association rules were tained.Crrelations between age,sex,electrocardiograph results,pathogeny and diagnosis of coronary heart disease were discovered.Conclusion Results from our experiments show that CAMD scans the database fewer times and has no need of pruning candidate itemsets.Thus,it greatly decrease the amount of useless rules produced and rapidly discover interesting association rules of medical data.

Objective To investigate the effect of salvia Miltiorrhiza on the patients with chronic allograft nephropathy.Method 20 cases were treated by the combination of salvia miltiorrhiza and routine anti-rejection therapy.Results The effect of the combination in salvia group was better than that in the control group .The significant difference was found between the two groups of the patients (P

Objective To explore the application effect of the model of clinical nursing path management in clinical nursing. Methods A comparative study was conducted of the clinical nursing paths respectively with regard to patients who had received thyroid adenoma resection and with regard to patients who had received hysteromyomectomy. Results In the experimental group involving clinical nursing path management, the average length of stay was shortened, pre operation days were reduced, and the average hospitalization expenses were lowered while the satisfaction rate of patients with thyroid adenoma rose by 2.18%, reaching 98.96% and that of patients with hysteromyoma rose by 2.87%, reaching 99.55%. At the same time, clinical nursing path management also enhanced the job satisfaction of nurses. Conclusion It is viable to adopt the model of clinical nursing path management in clinical nursing in China.

Objective To evaluate the effect of indomethacin in the prevention of post ERCP pancreatitis (PEP) .Methods The literatures and corresponding references of randomized controlled trials (RCT ) on indomethacin in the prevention of PEP were searched from Cochrane Library (Jan 1970 to June 2013) ,Pubmed(Jan 1966 to June 2013) ,EMBASE(Jan 1966 to June 2013)and China Biology Medicine disc(Jan 1978 to June 2013) .Literature screening ,data extraction and methodological quality assessment were individually performed by two researchers .The software RevMan 5 .2 of Cochrane Collaboration was used for statistical analy‐sis .Results A total of 7 trails ,2170 patients were included in this study .Meta analysis results showed:indomethacin could effec‐tively prevent from PEP (RR=0 .45 ,95% CI:0 .33-0 .60 ,P<0 .05) and Hyperamylasemia (RR=0 .51 ,95% CI:0 .37-0 .70 ,P<0 .05) compared with placebo ,and could effectively alleviate the severity of pancreatitis after ERCP (RR=0 .43 ,95% CI:0 .23 -0 .78 ,P<0 .05) .Conclusion Indomethacin could effectively prevent from PEP and Hyperamylasemia ,and could effectively alleviate the severity of PEP .

Humans ; Lymphocytes ; cytology ; drug effects ; Micronucleus Tests ; Occupational Exposure ; prevention & control ; Polycyclic Aromatic Hydrocarbons ; toxicity ; Population Surveillance ; methods

BACKGROUND:The mechanical environment of skin tissue and spreading state of epithelial cels are closely related with the wound healing and scar formation process. OBJECTIVE:To analyze the effect of extracelular mechanical stimulation on cel spreading, to test the cel proliferation in order to analyze the effect of spreading form on cel proliferation and other physiological activities. METHODS: Cyclic sine wave mechanical stretching was exerted on immortalized human keratinocyte by using FX-4000 flexible substrate loading system, on the condition of 0.2 Hz and at frequency of 10% amplitudes. The spreading form was compared at 0, 24 and 48 hours, the cel proliferation was analyzed with flow cytometry, and the distribution of vinculin was analyzed with immunofluorescence staining. RESULTS AND CONCLUSION: human keratinocyte would keep the spreading state and could induce more cel proliferation by 24 hours mechanical stretching stimulation. Conversely, after stimulated for 48 hours, the morphology of the human keratinocyte was significantly changed, and the number of human keratinocyte in the division stage was larger than that in the static control group; under tensile stress, the distribution of vinculin was transformed from the surrounding nucleus membrane area to the cel edge. The results indicate that proper mechanical stimulate can increase cel proliferation with keeping cel spreading and adhesion state; the stimulating time of continuous cyclic stretching is the major factor to determine cel spreading morphology and adhesion regions of immortalized human keratinocyte.

OBJECTIVE:To study the effects of carbamylated erythropoietin(CEPO)on cardiovascular microcirculation in rats with diabetes mellitus. METHODS:Rats were randomly divided into blank control group,model group,CEPO low-dose,medi-um-dose and high-dose groups(500,1 000,2 000 u/kg)with 12 in each group. The rats in the last 4 groups were reduced diabetes mellitus model. All rats were given relevant medicine intragastrically twice a week,coronary microcirculation endothelial cells were separated after consecutive 4 weeks. Enzyme-linked immunosorbent assay was conducted to detect levels of peripheral serum prosta-cyclin (PGI2),vasoconstrictor endothelin-1 (ET-1),angiotensin Ⅱ(AngⅡ) and von Willebrand factor (vWF) of rats in each group;in vitro CCK 8 test was used to detect endothelial cell activity(OD value);real-time quantitative polymerase chain reaction was adopted to detect proliferation-related genes(Ki67,p16),poptosis-related genes(Bad,Bax),and expressions of protein vas-cular endothelial growth factor(VEGF)and AngⅠ. RESULTS:Compared with blank control group,levels of PGI2,ET-1,AngⅡand vWF in serum in model group increased;OD value deceased;Ki67,p16,Bax and VEGF expression decreased;the difference was statistically significant (P<0.05). Compared with model group,levels of PGI2,ET-1,AngⅡ and vWF in serum in CEPO low-dose,medium-dose and high-dose groups increased;OD value increased;Ki67,p16 and VEGF expression increased;expres-sions of Bad and Bax decreased;the difference was statistically significant(P<0.05). The others had no significant difference(P>0.05). CONCLUSIONS:CEPO maybe improve the coronary microcirculation function by upregulating VEGF expression in coro-nary microcirculation endothelial cells and promoting endothelial cells’regeneration.

AIM: Tumor necrosis factor-?(TNF-?) participates in the establishment of inflammatory lesions in pneumonia.High production of TNF-? may relate to the severity of pneumonia.There have already been several studies examining the association between pneumonia and single nucleotide polymorphisms(SNPs) that affect cytokine productivity.SNPs of TNF-?,-1 031,-863,-857,-308 and-238 have been identified.The variant alleles of these SNPs have suggested to be related to high TNF-? production and the severity of pneumonia.Therefore,the aim of this study is to examine the association between the severity of pneumonia in Chinese and the following SNPs: five in the TNF-? gene promoter(-1 031,-863,-857,-308,-238).METHODS: A total of 117 Chinese individuals were enrolled in this study.They were 67 patients with pneumonia and 50 healthy subjects.TNF-? was genotyped by polymerase chain reaction-restriction fragment length polymorphism for all subjects.The frequency distributions of genotypes in different groups were analyzed by SPSS 11.5 program.RESULTS: Frequency of subjects who carried at least one variant allele in TNF-?-1 031,-863,-857,-308,-238 SNPs among pneumonia patients was significantly higher than that in healthy subjects.And frequency of subjects who carried variant allele in TNF-?-863,and-308 SNPs among severe adult community acquired pneumonia patients was significantly higher than that in common pneumonia patients.CONCLUSION: TNF-?-863 and-308 SNPs appear to be associated with severe adult community acquired pneumonia in Chinese populations.