Objective To study the etiology of surgical infection and antibiotic resistance of infective bacteria,so as to provide evidence for clinical treatment.Method The composition and antibiotic resistance in surgical specimens of pathogenic bacteria from January 2008 to December 2010 was analyzed retrospectively.Results A total of 321 pathogens isolated,Gram-negative bacteria 201,accounting for 62.6％,Gram-positive cocci 114,accounting for 35.5％,Fungus 6,accounting for 1.9％.Escherichia coli 116(36.1％),Klebsiella pneumoniae 24(7.5％),Staphylococcus aureus 34(10.6％),Pseudomonas aeruginosa 18(5.6％),Enterococcus faecalis 16(5.0％).Staphylococcus species of Staphylococci methicillinresistant Staphylococcus atureus(MRS),Coagulase-negative staphylococci MRS and ultra wide-spectrum beta-lactamases(ESBLs)detection rate was 52.9％(18/34),83.7％(36/43)and 35.0％(49/140),respectively.The resistance rates of Gram-negative bacilli to piperacillin/tazobactam,ceftazidime,amikacin,imipenem and meropenem were below 20％,Gram-positive cocci,staphylococci to new compound sulfamethoxazole,minocycline,rifampin,vancomycin,teicoplanin and fusidic acid resistance rates were less than 40％,various pathogens had varies resistance.Conclusions Specimens of surgical infection pathogens widely distribute in different drug-resistant bacteria,some strains of multi-drug resistant infection,causing clinical failure of empirical therapy,clinical laboratory susceptibility results should be based on the rational use of antibiotics,to avoid the overuse of antibiotics.

Objective:To investigate the association between anxiety and the change of humoral immune functions and its correlation with HLA-DQB1 polymorphisms.Methods:Total 31 resident doctors were selected randomly and tested by State-Trait Anxiety Inventory(STAI).IgG,IgA,IgM,complement C3 and complement C4 were detected with BECKMAN array360 system;HLA-DQB1*02、*03、*04、*05 and*06 alleles were individually amplified by polymerase chain reaction(PCR)using exon2 group-specific primers.The correlation between immune function and HLA-DQB1 polgmorphisms were investigated.Results:Statistical analysis showed that there was positive correlation with State Anxiety (Ta) and complement C3,either Trait Anxiety (Tc) and complement C3.There was significant difference between HLA-DQB1*02 positive and negative in Ta (P＜0.05),while no difference in complement C3(P＞0.05).There was significant difference between HLA-DQB1*04 positive and negative in Ta and Tc(P＜0.05),while no difference in complement C3(P＞0.05).Conclusion:Anxiety could change some humoral immune functions and this is related with HLA-DQB1 polymorphism.

To establish a new method for the determination of chondroitin sulfate. The method was based on the interaction of sodium chondroitin sulfate with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole to yield a violet product, 6-mercapto-3-substituted-s-triazolo-\[4,3-b\]-s-tetrzaine derivatives. Absorbance at 540 nm obeyed Beer's Law within the concentration range from 0.1 to 1.0 g?L~(-1)and the results were precise and accurate. The method was operationally simple and had a good precision and sensitivity.

Congenital majority teeth absence refers to the number of missing teeth up to 6 and above,which is rare in clinic.Most of these patients have syndromic symptoms of other organs,non syndrome type is more rare.This paper reports 2 cases of non-syndromic majority teeth congenital absence in mother and daughter,and makes related discussion.

Objective To observe the effect of Dahuangzhechongwan on protein excretion, serum cholesterol, TXB_2 and 6-keto-PGF_(1?) in rats with adriamycin-induced nephrosclerosis. Methods The rats received twice-intravenous injections of adriamycin after one kidney was removed, and were fed with hyperlipide food to establish an animal model for diffusion mesangioproliferation following with focal segmental glomerulosclerosis. The rats were randomly divided into shame-operated group, adriamycin nephrosclerosis group, Dahuangzhechongwan group, benazepril group. Urinary protein excretion, blood fat, TXB_2 and 6-keto-PGF_(1?) were observed. Result Proteinuria extract was decreased, the plasma cholesterol was significantly lower, the level of TXB_2 was cut down and 6-Ket-PGF_(1?) was increased by Dahuangzhechongwan. Conclusion Dahuangzhechongwan has the role of simutianeous application of elimination and tonification, promoting the circulation of Qi and blood, eliminating blood stasis, improving the hemodynamics. Dahuangzhechongwan has the renal protection on rat with adriamycin-induced nephrosclerosis, the mechanism may be relate with adjusting the balance of TXB_2 and 6-keto-PGF_(1?).

0.05). While the significant differences existed between postoperative hypoxemia group and non-hypoxemia group (P

Objective To study the influences of chitosan on swimming time, changes of blood glucose and lactic acid in mice. Methods The test groups were given chitosan by oral administration,and eighteen days later, the swimming time,blood glucose and lactic acid were determined. Results The swimming time of test groups was obviously longer than that of the control group.After thirty-minutes swimming, the elimination of blood lactic acid and the recover of blood glucose were distinctly faster than those of the control group. Conclusion Chitosan had anti-fatigue effect on mice.

Objective: To analyze the application value of radionuclide myocardial perfusion imaging in the localization and diagnosis for stable angina pectoris in clinical practice. Methods: A total of 70 patients who had been diagnosed with stable angina pectoris (SAP) were enrolled, and all patients underwent ATP-loaded ATP-99Tcm-MIBI (99Tcm-MIBI) myocardial perfusion imaging (MPI) and coronary angiography (CAG) were used to compare the accuracy of MPI imaging in localization and diagnosis for SAP. Results:In the 70 cases of patients with two methods of examination: ①In MPI diagnosis, positive patients were 50 cases, and the positive predictive value of SAP, sensitivity, negative predictive value and specificity were, respectively, 92.0%, 90.2%, 75.0% and 78.9%. There were 45 patients were consistently diagnosed as SAP by the two methods and the total compliance rate was 90.0%. ②in 70 patients, 51 cases were diagnosed as SAP by CAG examination, the positive rate was 72.9%; ③in 50 MPI positive patients, 46 patients were consistent with the results of CAG diagnosis, and the positive rate was 92.0%. The difference between the two methods was statistically significant (x2= 5.72, P<0.05). Conclusion: 99Tcm-MIBI myocardial perfusion imaging can be used as a gatekeeper in patients with stable angina pectoris, and has high application value in the location and diagnosis for stable angina pectoris. The diagnosis and treatment links of stable angina pectoris can save costs and provide a reliable evidencet for clinical practice.

Objective To evaluate the laparoscopic hysterectomy surgery continuous infusion of low-dose sufentanil combined with propofol and remifentanil anesthesia postoperative hyperalgesia influence.Methods Patients were collected from June 2013 to June 2015 in our hospital undergoing laparoscopic hysterectomy 60 cases.According to the principles of randomized, divided into sufentanil group (SF group) and 30 cases of normal saline group (NS group) 30 cases.Two intraoperative remifentanil 0.1-0.2 μg/(kg? min) and propofol 4-8 mg/(kg? h) to maintain anesthesia.After the operation began, SF group and NS group were sufentanil and saline.Record calls eyes, recovery of spontaneous breathing, extubation time;after waking 5 min, 30 min, 12 h, 24 h, 48 h pain (visual analogue scales, VAS) score; before induction (T0), after induction (T1), after intubation (T2), surgery (T3), extubation (T4), after extubation 10 min (T5) heart rate (HR) and mean arterial pressure (MAP); excessive sedation, respiratory depression, intraoperative awareness, restless when awake, intraoperative after nausea/vomiting, tramadol and so on.Results SF group and NS group recovery of spontaneous breathing, eye opening and extubation call time was not statistically significant;after SF group wake 5 min, 30 min and 4 h VAS scores lower than the NS group (P <0.05); in T4, T5 point comparison, SF group MAP and HR lower than the NS group (P <0.05); SF group restless when awake, pain (VAS score≥3), postoperative use of tramadol was lower than NS group (P<0.05).Conclusion Intraoperative continuous infusion of low-dose sufentanil improve laparoscopic hysterectomy in patients under anesthesia using propofol combined with remifentanil hyperalgesia, with good prospects for clinical application of anesthesia.