Due to the latent and highly invasive characteristics of liver carcinoma,a majority of patients are frequently not diagnosed until moderate or advanced stage in the disease process,and usually complicate with cirrhosis.Only 15％ -30％ of the patients are eligible for radical resection.The overall survival time is about 3-4 months.Therefore,on the basis of early diagnosis and treatment of liver carcinoma,how to improve the treatment effect of the moderate and advanced liver carcinoma,ameliorate the quality of life and prolong the survival time,becomes the focuses and difficulties both at home and abroad.

Objective:To identify the expression of egfp-hri fusion on NIH3T3 cells which is carried by recombinant retrovirus. Methods:The egfp-hri fusion gene was integrated intoNIH3T3 cells by the infection of recombinant viral supernatant from the G418 resistent PA317 cells transfected stably with the plasmid of pLNCX-EGFP-C1-hri. The expression effect of egfp-hri fusion was visualized under the fluorescent microscope, while the expression levels of egfp-hri fusion protein by Western blot. Results:The observation from fluorescent microscope showed the green fluorescent was obviously observed in the cytoplasm , western-blotting showed the expression level of egfp-hri fusion gene increased by 83%and 81%, as compared with those uninfected cells and in the cells infected with empty vector, respectively. Conclusion:The infected NIH3T3 cells can express egfp-hri fusion protein successfully.

Objective: To prepare and characterize Indigo Naturalis-Alumen (INA) composite particles with core-shell structure based on the particle design principle. Methods: The preparation time of shell particles and composition time of composite particles were studied using particle size and contact angle as evaluation indexes, respectively. Four kinds of powders including Indigo Naturalis fine powder, Alumen fine powder, INA mixture, and INA composite particles were prepared respectively; Their properties such as particle size, powder color, microstructure, distribution of surface elements, and crystal structure were compared, so as to study the core-shell structure of composite particles. Results: During the period of the preparation, the shell particles were added into vibromill and ground for 17 min separately, and then the core particles were added and ground with shell particles together for 5 min. There was no difference between the composite particles and shell particles in surface wettability. The median diameter (d0.9) of the composite particles was 32.818 μm, which was much smaller than that of the mixture. The color and surface elements distribution were highly similar to the shell particles. In SEM, the microstructure that the surface of big particles coated with a large number of small particles could be observed. In X-ray diffractogram, many characteristic absorption b ands of the core particles were disappeared or decreased. Conclusion: INA composite particles with core-shell structure are prepared successfully, the surface properties are similar to the shell particles and are different with the core particles.

Objective To investigate the clinical features and CT findings of cystitis glandularis.Methods The cystoscopic findings,clinical data and CT findings in 12 cases of cystitis glandularis patiens confirmed by cystoscopy and pathology were analyzed retrospectively with literatures review. Results The clinical symptoms included hematuria (8 cases),symptoms of urinary tract infection(3 cases) and dysuria(1 case).The lesions predominataly located at the trigone, neck of the bladder and around the entrances of the ureter.CT showed normality in 3 cases,bladder wall locally thickening in 7 cases( the ranges were 1.6～ 5.5 cm),extensive bladder wall thickening in 1 case. The lesions were isodensity on plain CT images and slightly enhanced after administration of contrast medium, the CT value increased 13.1 HU averagely.Conclusion Cystitis glandularis is of certain CT characteristics,however,no abnormalities are found by CT that can not be ruled out cystitis glandularis.

Objective To investigate the efficacy of intensity-modulated radiation therapy(IMRT)and the prognosis factors in advanced cervical cancer.Methods A total of 218 cases of cervical cancer patients treated in Affiliated Tumor Hospital,Guangxi Medical University,between July 2007 and July 2012,were divided into IMRT group and routine radiotherapy group (conventional group,94 vs 124 cases).To compare the short-term effects of two groups by Chi-square test,and the incidence rate of 1,3,5-year survival by rank sum test ; to compare the acute and chronic toxicity of the two groups by rank sum test.And to analysis prognostic factors by log-rank test and Cox proportional hazard model and multiple linear regression analysis.Results (1) The effective rate in IMRT group was 95.7％ (90/94),which in the conventional group was 86.3％ (107/124; P ＜0.01).The 1,3,5-year overall survival rates in IMRT group were not significant difference than those in conventional group (87.6％ vs 90.1％,73.4％ vs 81.5％,and 72.3 ％ vs 75.7％ ;all P ＞ 0.05).The rate of acute gastrointestinal toxicity was 41.5 ％ (39/94) in IMRT group,vs 42.7％ (53/124) in conventional group(P ＜ 0.01); and the incidence of acute hematopoietic toxicity in IMRT group was 76.6％ (72/94),which was 78.2％ (97/124) in conventional group (P ＜0.01) ; the incidence of acute genitourinary toxicity was 12.8％ (12/94) in IMRT and 8.1％ (10/124) in conventional group(P =0.248).The late gastrointestinal,genitourinary and hematopoietic toxicities were 18.1％ (17/94),16.0％ (15/94) and 25.5％ (24/94),which were lower than those in conventional group 91.9％ (114/124),47.6％ (59/124) and 56.4％ (70/124),respectively (all P ＜0.01).(2) Univariate analysis showed that the International Federation of Gynecology and Obstetrics (FIGO) stage,histological grade and tumor size were closely associated with 5 years disease-free survival rates and overall survival rates (all P ＜ 0.05).Multivariate analysis showed that FIGO stage and histological grade were closely associated with 5 year overall survival rates (all P ＜ 0.01).FIGO stage and histological grade were independent factors to patients' 5-year overall survival rates in cervical cancer (P ＜0.01).Conclusion IMRT would be to reduce the acute and chronic toxicity,and to improve the quality of patients' life,but did not improve the 5 years survival rate in advanced cervical carcinoma.

BACKGROUND:It is difficult to make an exact conclusion about the correlation between atherosclerosis and hemodynamic wal shear stress because of the complexity of the hemodynamic environment in the field of atherosclerosis research.
OBJECTIVE:To analyze the relationship between characteristics of mural coronary artery hemodynamics parameters and atherosclerosis through myocardial bridge-coronary artery analog device.
METHODS:Myocardial bridge-coronary artery analog device was used to simulate experiments in vitro. In the condition of keeping any related parameters unchanged, such as system temperature, the average flow rate and heart rate, we observed and recorded the changes of proximal and distal mean value and oscil atory value of shear stress of mural coronary artery by regulating the oppression level of myocardial bridge to mural coronary artery.
RESULTS AND CONCLUSION:Under 0%oppression, there was no significant difference in distal and proximal mean value and oscil atory value of shear stress of mural coronary artery. Under 50%oppression, the proximal oscil atory value of shear stress was significantly larger than that in the distal end. Under 80%oppression, mean shear stress in the distal end was larger than that in the proximal end. Oscil atory value of shear stress in the proximal end was larger than that in the distal end. With increased oppression, the mean shear stress in the distal end was higher than that in the proximal end, but oscil atory value in the proximal end was higher than that in the distal end. These results indicated that the increase of the oscil atory value of the proximal shear stress of mural coronary artery is an important factor for the occurrence of atherosclerosis.

Objective:To identify the expression of plasmid pLNCX-EGFP-C1-hri targeting the gene of Human ribonuclease inhibitor (hri) in PA317 cells which is capable of expression in mammalian cells.Methods: The vector of pLNCX-EGFP-C1-hri was transfected into PA317 cells by Lipofectamine 2000 and then the expression of recombinated plasmid was verified in living cells by observing the transcription level of egfp-hri fusion gene mRNA with RT-PCR method and the expression level of egfp-fusion hRI protein with western blotting method respectively.Results: Both RT-PCR and western blotting showed the egfp-hri fusion gene was obviously expression in PA317 cells.Conclusion: The plasmid of pLNCX-EGFP-C1-hri targeting hRI is successfully constructed and the protein of hRI can be expressed in PA317 cells correctly.

Heart failure is one of the most common acute and critical disease in pediatric,early diagnosis of diseases is an important cli-nical value.The current study found that brain natriuretic peptide(BNP)is a peptide hormone,which is secreted mainly by ventricle and may reflect the state of cardiac function;BNP level of detection is contributive to the diagnosis,treatment of heart failure,etc.J Appl Clin Pediatr,2009,24(1):64-66

Objective To study CT value in diagnosis and differential diagnosis of submandibular tumor.Methods The CT features of submandibular tumors proved by pathology in 20 cases were analyzed retrospectively and compared with pathologic findings. Double-blind was used to evaluate the value of CT in differentiating both malignant and benign submandibular tumors. Results Of 20 patients,8 were benign tumors, 6 appeared as homogeneous density and well-defined, 2 appeared as inhomogeneous density and partial ill-defined.12 case were malignant of them, 10 were irregular in shape, 8 were heterogeneous indensity, 7 were ill-defined, 5 were well-defined in shape, 4 were homogeneous indensity. Double-blind indicated that 75% submandibular tumors could be qualified by CT, but could not determine the histological type of tumors. Conclusion CT can differentiate most of malignant submandibular tumors from benign tumors, but can not be used to categorize the histological type of tumors.

Objective To analyze CT features and CT diagnostic value in lymph node reactive hyperplasia.Methods CT findings of lymph node reactive hyperplasia in 13 cases proved surgically and pathologically were retrospectively analyzed.Results Of 13 cases,7 cases were only involved cervical lateral lymph nodes,2 cases were simultaneously involved cervical lateral,facial and submental lymph nodes,1case was involved axillary and inguinal lymph nodes,1 case demonstrated simultaneously enlarged in cervical,axillary,mediastinal,retroperitoneal and inguinal lymph nodes.Enlarged lymph nodes ranged from 0.6 to 2.6 cm in minimal diameter.with mean of 1.6 cm.Lymph nodes enlaged showed isolated existence in 12 cases,merely 1 case mixed existence and the density was unhomogeneous,12cases showed homogeneous density and obvious enhancement on postcontrast CT,and CT value increased by 19.1~113.2 HU,with mean of 59.1 HU.Conclusion Lmyph node reactive hyperplasia is of characteristic CT appearances,CT examinations is of important value for its qualitative and differential diagnosis.