Gastric cancer(GC) is deeply threatening the health of our people, the mortality rate of GC is the second death cause of carcinoma i n our country. Since the lack of special clinical symptom at the early state of GC, detecting GC at the molecular biological level is expected to diagnose early gastric cancer. This article discusses some markers such as PCNA, p53, telomera se, ras protein and so on to assess the possibility of diagnosing the early gas tric cancer with these molecular biological markers. According to analyzing rela ted papers published, PCNA and p53 were found to be the molecular biological mar kers which should be firstly taken into account in diagnosing gastric cancer. Bo th of them can be easily detected by using immunohistochemical method. Telomeras e is also a good marker, but because of its difficult technology and high cost, it is not so practicable as the former two as the diagnostic marker of early gas tric cancer. Besides, ras potein can also be taken into account as the early det ecting auxiliary marker for GC.

Changes in heart rate of fetal is function regulating performance of the circulatory system and the central nervous system, it is significant to detect heart rate of fetus in perinatal fetal. This paper puts forward the fetal heart rate detection method based on short time Fourier transform and blind source separation. First of all, the mixed ECG signal was preprocessed, and then the wavelet transform technique was used to separate the fetal ECG signal with noise from mixed ECG signal, after that, the short-time Fourier transform and the blind separation were carried on it, and then calculated the correlation coefficient of it, Finally, An independent component that it has strongest correlation with the original signal was selected to make FECG peak detection and calculated the fetal instantaneous heart rate. The experimental results show that the method can improve the detection rate of the FECG peak (R), and it has high accuracy in fixing peak(R) location in the case of low signal-noise ratio.
Electrocardiography ; Fetus ; Heart Rate, Fetal ; Humans

Objective To investigate the changes of blood cell distribution width and D-dimer in patients with chronic obstructive pulmonary disease.Methods 60 cases with chronic obstructive pulmonary disease were selected and according to the course of disease were divided into two groups.30 healthy people were selected to be the control group.MCV, RDW and D-dimer were detected and compared.ResuIts Compared with the control group, MCV and RDW of acute phase group and remission stage group are higher(P<0.05), compared with the remission stage group, MCV and RDW of acute phase group were higher(P<0.05), and RDW and MCV in the acute stage group were positively correlated with the remission stage group (r=0.717, 0.902, P<0.05).Compared with the control group, D-dimer of acute phase group and remission stage group were higher(P<0.05), compared with the remission stage group, D-dimer of acute phase group were higher(P<0.05), and D-dimer in the acute stage group were positively correlated with the remission stage group (r=0.727, P<0.05).ConcIusion The distribution of blood cell distribution width and D-dimer levels in patients with chronic obstructive pulmonary disease in acute stage and remission stage group were increased, and the increasing of patients with chronic obstructive pulmonary disease in the acute stage is more obvious.

A computerized fetal ECG(FECG)system is presented,w hi ch is composed of two components.One is the data collection device with a PIC m icrocomputer as the main hardware,which has low power consumption and gets power supply through serial port.The other is a software platform based on PC,which i s built through LabView6.1,a graphic programming language.Plenty of modules prov ided by LabView6.1utilized,it is efficient to develop the software platform.In particular,the implementation of the matched filtering algorithms is introduced ,which is the core of the software platform.A new method of updating templates o f matched filter is described.

BACKGROUND: The meniscal allograft transplantation has been proved good outcome in clinical, however, the source and matching restrict its application. By means of a patent technique of separating antigen, a new biological meniscus can be obtained.OBJECTIVE: To observe the histological changes of transplanted biological meniscus in goat's knee joint, in addition, to investigate the feasibility of reconstituting meniscus by using biological meniscus. DESIGN, TIME AND SETTING: The animal experiment of goats was performed at the Central Laboratory in the Third Affiliated Hospital of Sun Yat-sen University between June 2005 and June 2006.MATEAIALS: A total of 15 healthy goats were used in the experiment. Fresh porcine meniscus was harvested for the preparing of biological meniscus.METHODS: The medial meniscus of goat was excised, and then the absent part was repaired using biological meniscus. MAIN OUTCOME MEASURES: The animals were sacrificed at months 1, 3, 6 and 12 after operation. And the samples were detected by histology, scanning electron microscopy (SEM) and Indian ink perfusion. RESULTS: The incision of all animals healed well, without infection or death. The transplanted meniscus healed well with the surrounding tissue, and the connection was tight. With time prolonged, the transplanted meniscus slowly soaked, and live cells existed inside the meniscus, but there were no live cells in about 1/4 district at 1 year after the surgery. Indian ink perfusion showed that the surrounding tissue grew inside and set up microcirculation, but only limited to 10% periphery of the meniscus (normal goat approximately 15%). The transplanted meniscus began the necrosis at 6 months after surgery. Under SEM, the surface of the meniscus became crude and cracked in some places, but still maintains the gross shape at 1 year after the surgery.CONCLUSION: There is no obvious rejection after the biological meniscus was transplanted into knee joint of goats. During the process of absorption, autologous tissue of goats can grow into and set up microcirculation in the transplanted meniscus. The transplanted meniscus protects the'knee cartilage notably, only slightly damage in cartilage can be observed.

[Objective]To determine the incidence and factors associated with pyrexia after total knee replacement(TKR).[Method]A retrospective review of 200 patients in departments of orthopaedics of our hospital and West China hospital who underwent TKRs from August 1998 and April 2006 was performed.The clinical,surgical and follow-up data were abstracted from the patients' records and were analyzed with SPSS 12.0.[Result]There was a statistically significant increase of the mean temperature post-operations,which remained significant throughout the first 7 days after surgeries(P

Objective To investigate effect of autologous hematopoietic stem cell transplantation on serum trace elements and lactate dehydrogenase activity in patients with malignant lymphoma.Methods 80 patients with malignant lymphoma collected in our hospital from May 2013 to May 2014,40 cases in each group, Hodgkin lymphoma ( HL) patients were treated with ABVD ( doxorubicin, bleomycin, vincristine, dacarbazine) regimen of chemotherapy; Non-Hodgkin,s lymphoma ( NHL) patients were treated with standard CHOP ( cyclophosphamide, vincristine, epirubicin, dexamethasone) regimen of chemotherapy of two group.Chemotherapy drugs combined with granulocyte colony stimulating factor ( G-CSF) was used to mobilize APBSC.Application of 10μg/(kg? d)G-CSF 5 in bone marrow suppression after chemotherapy, detected white cell increased 3.0 ×109/L , platelets rose to 50 ×109/L, collected APBSC,The frozen stock solution of cell was joined and frozen.24 h after pre-treatment, the frozen peripheral blood hematopoietic stem cells of the subclavian vein was transfused for patients in experimental group.Control group continued to receive chemotherapy. After treatment, the Cu level, Zn level and lactate dehydrogenase activity were detected in all patients.ResuIts After treatment, Zn levels of two groups increased (P<0.05), Cu and Cu/Zn levels decreased (P<0.05).Compared with control group, the level of Zn was higher in experimental group (P<0.05), and the level of Cu and Cu/Zn was lower (P<0.05).After treatment, the lactate dehydrogenase activity of two groups decreased (P<0.05), and experimental group was more lower than control groupy (P<0.05).ConcIusion Autologous hematopoietic stem cell transplantation can significantly reduce the level of Cu in patients with malignant lymphoma , elevate Zn level and decrease the activity of lactate dehydrogenase, which has guiding significance for clinical.

Objective To evaluate the clinical effects of AO clavicular hook plates in treatment of dislocation of acromioclavicular joint and/or fracture of distal clavicle in comparison with traditional means. Methods A retrospective analysis was done to compare the outcomes and complications between the treatment of dislocation of acromioclavicular joint and/or the fracture of distal clavicle by AO clavicular hook plates and the treatment by conventional devices (e.g. Kirschner’s wire, wire-cerclage, bolt, et al). Results 40 cases were followed up for 12 to 20 months and the average time was 14 months. According to Lazzcano’s criterion, the rate of excellent and fine of the clinical outcomes of the 2 groups was 100%and 78.6%respectively. After statistical Chi-square test, P=0.025. Therapeutic outcomes and complications of the former were obviously better than the latter. Conclusion Since AO clavicular hook plates display advantages of rigid fixation, non-invasion into articular surface, and possibility of early functional exercise in the treatment of acromioclavicular dislocation and fractures of distal clavicle, they are worthy of recommending.

Bee/wasp sting associated ischemia stroke is very rare.Its early clinical manifestation,especial level of consciousness,is serious than general cerebral infarction.There is no specificity with laboratory examination.Most of the infarction lesions involve two or two more regions detection by brain computerized tomographic scanning/magnetic resonance imaging (CT/MRI).Parts of the occlusion vessels could recanalization.Therapy with hormones and antihistamine has certain effect.Most of patients have approving prognosis.The pathological mechanism is mainly associated with doctrine such as vascular inflammation,vascular spasm,immune hyperfunction,brain tissue hypoperfusion and retrograde stimulation of the superior cervical ganglion.

Objective To investigate the relationship between HBV genotypes and YMDD motif mutations or BCP mutations in Xiangtan of Hunan Province. Methods HBV genotypes, YMDD motif mutations and BCP mutations were analyzed in 952 HBV infected patients. Results HBV genotyping showed that 698 HBV type B patients and 115 HBV type C patients accounted for 73.32% and 12.08% respectively of all the participants. The rest 139( 14.60% )were genotype B and C mixed infection( B + C ). The analysis of YMDD motif mutations showed that 844 YMDD wild-type which accounted for 88.66% of all the subjects and the remainder were YMDD mutation types, of which 54( 5.67% ) carried YVDD, 53( 5.57% ) YIDD,and 1 YVDD and YIDD mixed infection. Basic Core Promoter mutations showed that 1762A/1764G ( wild type )accounted for 70.59% and 1762T/1764A( mutant ) accounted for 19.75%. The rest 92 patients were 1762T/1764A and 1762A/1764G mixed infection. This study showed no significant difference in the rate of YMDD mutation( 10.04% vs 10.43% ,χ2 =0.017,P＞0.05 ) ,but a significant difference in the types of YMDD mutation(χ2 = 4.836, P ＜ 0.05 )between HBV types B and C. The YVDD mutation was more commonly seen in genotype C( 9.57% ) than in genotype B( 5.88% ). The BCP mutation rate showed a significant difference( 27.36% vs 46.09%, χ2 = 16.478, P ＜ 0.01 ). Genotype C was more frequent than genotype B. The BCP mutation rate showed no significant difference between YMDD Wild-type and YMDD mutation types( 28.67% vs 35.51%, χ2 = 2.139, P ＞ 0.05 ), but most of BCP mutations happened in YVDD mutant type( 61.11% ). Conclusions ( 1 ) The predominant HBV genotypes in Xiangtan were genotype B and genotype C, the major genotype was type B, which display the characteristics of epidemiology in Southern China. ( 2 ) Determination of HBV genotypes before lamivudine therapy was probably not an important pretreatment investigation to predict antiviral responses. ( 3 ) Detection of HBV genotypes, YMDD motif mutations and BCP mutations will contribute to the correct evaluation of prognosis and timely proper management of HBV patients.