The method of ratio coefficient of amino acid, based on the theory of amino acid balance, is a new one designed to evaluate nutritional value of food protein. The calculation and the meaning of Ratio Coefficient (RC) and Score of RC (SRC) are described. RC can be used to judge limit amino acid and to calculate the amount that shall be added to a fortified food. SRC can be used to evaluate nutritional quality of protein. A high correlation was obtained between SRC and Biological Value (BV), P

Objective To investigate the effect of salvia Miltiorrhiza on the patients with chronic allograft nephropathy.Method 20 cases were treated by the combination of salvia miltiorrhiza and routine anti-rejection therapy.Results The effect of the combination in salvia group was better than that in the control group .The significant difference was found between the two groups of the patients (P

ObjectiveTo compare the early rehabilitation of child with brain impair syndrome and cerebral palsy executed in hospital and in family.Methods180 children of 0～2 years old with brain impair syndrome and cerebral palsy were divided into group A and group B randomly. The children of 0～6 months old were in group A1 and group B1, while that of 6 months～2 years old were in group A2 and group B2, correspondingly. Children in group A accepted early formal strengthen rehabilitation training in hospital, and that in group B accept family rehabilitation through guidance. Development quotient (DQ) were assess with Japan Yuanchengsi infant analysis growth scale half a year after.ResultsThe DQ of group A showed higher than that of group B(P<0.01), and DQ of group A1 was higher than DQ of group A2(P<0.05).ConclusionEarly rehabilitation training in hospital is more effective on early cerebral palsy.

Objective To identify the correlative and risk factors of non-oxygenation factors associated with postoperative prolonged mechanical ventilation (PMV) of aortic dissection(AD).Methods AD patients undergoing surgery during January 2010 and January 2015 were enrolled.Prolonged mechanical ventilation was defined that duration of ventilation more than 48 h.Results There were 240 patients,average age was (50 ± 12)years.The correlative factors with postoperative PMV were:pre-opervative white blood cell (WBC) (r =0.241,P =0.003),emergency operation (r =0.263,P =0.004),Debakey type (r =-0.379,P =0.000),duration of operation (r =0.329,P =0.000),postoperative diastolic pressure (r =-0.205,P =0.007),heart rate (r =0.246,P =0.001),postoperative hemoglobin (r =-0.213,P =0.005),calcium(r =-0.262,P =0.001),glucose (r =0.274,P =0.000),lactate(r =0.272,P =0.000) and pericardial effusion (r =0.239,P =0.032).Logistic analysis indicated that:the duration of operation,WBC and postoperative blood calcium were:2.063,1.285,0.016,respectivly(all P ＜ 0.05).Conclusions The correlative factors were:preoperative WBC,emergency operation,Debakey Type,duration of operation,heart rate,postoperative diastolic pressure,hemoglobin,calcium,glucose,lactate,and pericardial effusion.Duration of operation,WBC and postoperative blood calcium were risk factors predicting PMV.

Neovascular age-related macular degeneration(nAMD)and polypoidal choroidal vasculopathy (PCV)are leading causes of blindness and visual impairment in older population.They are both with a hemorrhagic and exudative macular disorder.PCV has been proposed to be a sub-type of nAMD,hut this is a matter of controversy.Although several genes are found to be associated with PCV and nAMD(CFH,ARMS2/HTRA1),but more genes show no common association with the two diseases.such as BF/C2,Elastin,PEDF,SERPlNG1,VEGF,APOE genes.All these Results might imply that PCV and nAMD have different pathogenesis.But in the current state of our knowledge for PCV and nAMD.we still could not conclude if they are the same diseases.The purpose of these review is to discuss the recent progress in the molecular biology of PCV and nAMD.

This paper analyzed the main problems in the thesis of postgraduates with specialized master degree of public health.The problems existed were the poor quality of students,untargeted topics of the thesis and unsound evaluation system,etc.Specific measures were proposed for the construction of quality assurance system for postgraduates with specialized master degree of public health including four focus management,multiple evaluation system and post thesis evaluation.

AIM　To study the effects of vitamin E succinate (VES) on the cell growth and the DNA synthesis of human gastric carcinoma cell (SGC-7901). METHODS　The growth curve was determined with counting viable cell numbers. The colony formations were counted with Giemsa dye staining. The cell cycle was analyzed using flow cytometry (FCM) and the DNA synthesis was observed with the 3H-TdR incorporation method. RESULTS　VES could inhibit the growth and colony formation of SGC-7901 cells. Growth curve display：after SGC-7901 cells were treated with 5 mg*L-1、10 mg*L-1 and 20 mg*L-1 VES for seven days, the inhibition rate are 41.2%、98.3% and 100%, respectively. The colony formation of SGC-7901 cell at 24 h was inhibited 6.7%、50.4%、87.2%, and at 48 h was 24.7%、73.4%、100%, respectively. FCM analysis revealed that VES could decrease the percentage of cells in G2-M phase after treated 48 h in a dose-dependent manner, while increase the percentage of cells in S pheise. The assays of 3H-TdR incorporation into DNA showed obvious inhibition dose-dependently after exposure to VES for 48 h. CONCLUSION　VES could inhibit gastric carcinoma cell growth by arresting DNA synthesis in vitro.

Objectives To evaluate the mechanism of mitral regurgitation after mitral valve surgery by quantitative assessment of mitral valve three-dimensional parameters using real-time three-dimensional echocardiography (RT-3DE). Me-thods According to the improved degree of mitral regurgitation (MR), 30 children were divided into two groups after mitral valve repair:MR improved (I) group and residual MR (II) group. And the pre-operative 3-DE parameters were compared with post-operative parameters in two groups respectively. Results In group I, three dimensional parameters, including mitral valve annular area in end-systole and end-dilate (AA1, AA2), PM papillary muscle annular distance (AL-A), AL papillary muscle annular distance (PM-A), inter-papillary distance (PM-AL), the anterior leaflet and posterior leaflet tethering angles (a1, a2, argAL-C-PM), left ventricular volume end-systole and end-dilate (LVEDV, LVESV) were significantly improved after surgery (P<0.05) while the parameters PM-A, PM-AL, argAL-C-PM, LVEDV, LVESV and left ventricular ejection fraction (LVEF) were not significantly changed after surgery in group II, except for the parameters AA1, AA2 and a2 (P>0.05). Conclusions Most of the mitral valve parameters were much better in group I after surgery. However, the malformation of papillary appa-ratus, leaflet tethering strength and left ventricular function were unchanged in group II, which may be the key mechanism of mitral regurgitation after surgery.

Objective To establish a animal model for diffusion mesangioproliferation complicating focal segmentally glomerulosclerosis.Methods The models were established in the seventieth day by repetitive injection of 4mg/kg and 3mg/kg adriamycin to the SD rats in the 7th and 30th day after removal of their right kidneys.Results Serious edema, profuse albuminuria, hypoalbuminemia, hyperlipemia and renal failure were presented. Simultaneously, diffuse medium- or serious-grade proliferation of mesangial matrix, slight-grade proliferation of mesangial cells, segmental sclerosis of 22% of glomeruli, and overexpression of PDGF-B and bFGF in glomeruli were showed by pathologic examination, computerized image analysis and immunohistochemistry staining.Conclusions The models established in the transition stage from mesangioproliferation to glomerulosclerosis are helpful to study mechanisms and take measures for prevention and cure of chronic glomerulonephritis.

[Objective]To evaluate the results of intraoperative autologous blood transfusion in pediatric orthopaedic operation.[Method]Intraoperative blood salvage was reinfusioned in 17 patients by Cell Saver 5.The volume of reinfusion blood and complications were recorded.[Result]Among 17 patients,an average of(171?53)ml autologous blood was transfused intraopratively.The use of Cell Saver 5 was directly responsible for a 80% reduction in the total amunts of homologous blood.Four patients has experienced transient hemoglobinuria.No other complications appeared.[Conclusion]Reinfusion of blood salvage during pediatric orthopaedic operation is safe and effective.It is able to reduce the amount of homologous blood transfusion.