A method of preparation of Chondroitin su(?)fate from Raja porosa cartilage was presented, using the extraction process of increasing concentration of strong bases, the hydrolysis of complex enzyme and the alcohol precipitat(?)on method. The technologic condition was researched. The white powder of Chondroitin sulfate was obtained at the yield rate of 2.82% and the purity of 93.6%.

Objective:To investigate the association between anxiety and the change of humoral immune functions and its correlation with HLA-DQB1 polymorphisms.Methods:Total 31 resident doctors were selected randomly and tested by State-Trait Anxiety Inventory(STAI).IgG,IgA,IgM,complement C3 and complement C4 were detected with BECKMAN array360 system;HLA-DQB1*02、*03、*04、*05 and*06 alleles were individually amplified by polymerase chain reaction(PCR)using exon2 group-specific primers.The correlation between immune function and HLA-DQB1 polgmorphisms were investigated.Results:Statistical analysis showed that there was positive correlation with State Anxiety (Ta) and complement C3,either Trait Anxiety (Tc) and complement C3.There was significant difference between HLA-DQB1*02 positive and negative in Ta (P＜0.05),while no difference in complement C3(P＞0.05).There was significant difference between HLA-DQB1*04 positive and negative in Ta and Tc(P＜0.05),while no difference in complement C3(P＞0.05).Conclusion:Anxiety could change some humoral immune functions and this is related with HLA-DQB1 polymorphism.

In the hospital administration,the establishment and the implementation of the strategic planning of the hospital occupies a very important position.This paper,combined with the actual situation of a children's hospital in Chongqing,describes the hospital rational use of three level management system of the hospital,office,department,in order to get the full investigation of the national macro policy,the development of the medical industry and the status of the hospital and other aspects,and then supports efficient workflow to complete the hospital strategic planning.This paper also focuses on the target implementation,program implementation and project implementation of three levels,in order to illustrate the process for the implementation of the strategy to provide a reference of.hospital strategic planning formulation and implementation.

Humanism care meets the need of the development of the whole society, the need of transformation of modern medicine pattern it is also the great need for uremia patients who thirst for health.Uremia patients with psychological distress will be greatly improved through following aspects: enhancing quality of medical staff,making more communications with patients and providing health education to them.In a word,humanism care for uremia patients will make them feel more love and improve their living states.

Objective:To observe the clinical efficacy of electroacupuncture and rehabilitation exercises in the treatment of cerebral infarction.Methods:Seventy-four patients with cerebral infarction were randomized into treatment group and control group in visit sequence.Thirty-seven cases in treatment group were treated with electroacupuncture and rehabilitation exercises,and other 37 cases in control group were treated with electroacupuncture alone.The treatment was given once a day and there was a 2-day interval after any consecutive 5 treatments.After total 30 treatments,Fugl-Meyer scale was used to evaluate motor function and Barthel Index to evaluate the activities of daily living.Results:After treatments,the motor function of paralyzed limb and activities of daily living were obviously improved in both groups,with more significant improvement in treatment group than in control group (P＜0.05).Conclusion:Combination of electroacupuncture and rehabilitation exercises has better effects than simple electroacupuncture in the treatment of cerebral infarction.

Objective To investigate the clinic effect of hypocellular acute myelogenous leukemia (AML) patients with CAG regimen. Methods 29 cases with hypocellular AML were treated with CAG regimen. Results There were 14 cases achieved complete remission (CR), accounting for 48.3 %; 7 cases partial remission (PR), accounting for 24.1%. The total effective rate was 72.4 %, inducing 7 cases of NR, one case dead in early time. Conclusion CAG regimen has been proved to be effective in shortening the period of reduced peripheral blood granulocytes and decreasing the death rate related to chemotherapy, hence,a secure and effective therapy in treating hypocellular AML.

Objective To investigate the expression of CIP2A and c‐Myc and their correlation in cervical carcinoma .Methods We detected CIP2A and c‐Myc expression using immunohistochemistry in 72 samples of cervical carcinoma and 12 samples of normal cervix tissues .Correlation between proteins and clinicopathologic features and relation between CIP2A and c‐Myc expression were analyzed .Results The positive expression of CIP2A and c‐Myc in cervical carcinoma tissues were 52 .8% and 56 .9% ,respec‐tively .While their positive expression in normal cervix tissues were 8 .3% and 25 .0% ,respectively .The differences had statistical significance(χ2 =8 .169 ,P=0 .004 ;χ2 =4 .208 ,P=0 .040 ,respectively ) .Clinicopathological analysis suggested that CIP2A and c‐Myc protein expression were associated with histopathological differentiation and clinical stage(P<0 .05) in cervical carcinoma ,but the protein expression was not related to age ,lymph node metastasis and pathological type .CIP2A was significantly positive correla‐ted with c‐Myc protein in cervical carcinoma(r=0 .673 ,P=0 .001) .Conclusion The high‐expression of CIP2A is correlated with malignant clinicopathologic characteristics ,and CIP2A is positively associated with c‐Myc ,suggesting that CIP2A may promote tumor initiation and development through maintaining c‐Myc protein in cervical carcinoma .

The pathogenesis of diabetic retinopathy (DR) is more complex. For the upstream of traditional pathogenesis, to looking for unifying mechanism theory which proposed in foundation of common promoters and the latest view of DR may be the result of chronic inflammation. Both of them provide the basic and clinical theraby of DR with new direction. Therefore, there are many related issuess till needs to intensive study.

AIM To study the effect of Topotecan(TPT) on cell cycle and apoptosis of hunman lung cancer SPC A 1 cells and the role of Caspase 3 and bcl 2 involved in TPT induced apoptosis. METHODS Cells were incubated with TPT(5,10,15 and 20 mg?L -1 ) for 24 h. Apoptosis was detected by TUNEL staining, electronic microscope and DNA agarose gel electropheresis. Flow cytometer was used to analyse cell cycle changes and to detect apoptotic rates and bcl 2 expression of cells treated with TPT or TPT and 50 ?mol?L -1 Ac DEVD CHO(specific Caspase 3 inhibitor). RESULTS Apoptosis of cells treated with TPT(5 mg?L -1 ) was not detected, but apoptosis rates increased with the increase of TPT concentration and there was significant difference between each concentration group( P

Objective To investigate the effects of hyperoxic-hyperosmotic sodium chloride solution(HHSCS) on arterial blood gas and blood lactate in rabbits with hemorrhagic shock, and evaluate the HHSCS effects on hemorrhagic shock resuscitation.Methods Hemorrhagic shock models were induced in 30 male rabbits.The median arterial pressure(MAP) was declined to 40mmHg within 10 minutes,then maintained for 60(minutes).The animals were then randomly divided into 3 groups:normal saline hyperoxic solution(NSO)group,hyperosmotic sodium chloride solution(HS)group and HHSCS group.Sixty minutes later,NSO,HS or HHSCS 6ml/kg was infused intravenously in 5 minutes.Heart rate(HR) 、respiratory rate(RR)、 MAP and urine drops(UD) before shock and after fluid infusion were recorded;blood lactate(BL) and blood gas were measured before shock,60min after shock,and 30min,60min,120min postinfusion.Finally,the(rabbits) were killed by bleeding,the lungs were observed in autopsy,and the lung coefficient was measured.Results HS and HHSCS more effectively raised MAP,enhanced heart function,and increased UD than NSO.HHSCS group significantly reduced BL.At 60,120min after infusion,SaO_2 in HHSCS group was(significantly) higher than that in HS group and NSO group(P