Objective: Sponges (phylum Porifera) are sessile marine invertebrates and are known to be the richest source of pharmacologically-active compounds. This work was taken to investigate the antibacterial, antifungal activity and cytotoxicity from marine sponge. Method: In this study the marine sponge Spongosorites halichondrioides crude extracts were investigated for three bioassays. The first is an antimicrobial test against Proteus vulgaris, Bacillus subtilis, Staphylococcus aureus, Salmonella typhi, Klebsiella pneumonia, Escherichia coli, P. aeruginosa and the second is an antifungal test against three pathogenic fungi, Aspergillus flavus, Aspergillusniger and Metarhizium anisopliae. The third is a cytotoxicity test using larva of Artemia salina, for detection of cytotoxic activity in the extracts. Result: For all the three bioassays, extracts were found to be bioactive. This result suggests that this marine sponge is able to produce biologically active agents required for an overall defense against their predators. Conclusions: Further GC MS was done and the fragmentation pattern, showed the presence of sterol esters and terpenoids in the active extracts.