Objective This aim of this study was to understand the distribution of pretreatment serum LDH in NPC of each radiotherapy-related typing,and its relationship with prognosis.Methods We collected the pretreatment data of LDH in 2 665 newly diagnosed NPC who underwent radical radiotherapy between January 1,2000 and December 31,2005 at Sun Yat-sen University Cancer Center.Pretreatment serum LDH levels and survival analysis were analyzed in four kinds of radiotherapy-related typing of NPC.Results Distribution of radiotherapy-related types of 2 665 NPC were 74.6％ (1 987 cases),15.1％ (404 cases),8.6％ (229 cases),1.7％ (45 cases) for Types Ⅰ (no primary and regional recurrence and no distant metastasis),Ⅱ (primary or regional and no distant metastasis),ⅢⅡ (no primary and regional recurrence,and distant metastasis),Ⅳ (primary or regional recurrence,and distant metastasis),respectively.Increased LDH patients Ⅰ,Ⅱ,Ⅲ,Ⅳ-type proportion were:9.6％ (191/1 987),15.8％ (64/404),18.8％ (43/229),35.6％ (16/45).274 cases of type Ⅲ and Ⅳ,there were 81 cases of liver metastasis,which increased LDH levels in 34 cases,accounted for 42％.Total follow-up rate was 95.2％.The OS,LRFS,and DMFS curves for the increased LDH level group and the normal LDH level group was significant (P =0.000,0.000,0.000).Further analysis showed that the above main difference caused by the type Ⅱ (P =0.000).Conclusions Pretreatment levels of serum LDH are associated with radiotherapyrelated typing of NPC,particularly associated with type Ⅲ and type Ⅳ liver metastasis.And mainly type Ⅱ has poor prognosis.

Objective To investigate the therapeutic mechanism of Gandou Bushen Decoction(GDBSD)for improving reproductive disorders in male mouse models of Wilson disease(WD).Methods Sixty male homozygous TX mice were randomized equally into 4 groups and treated with daily gavage of saline(WD model group),penicillamine(0.09 g/kg),or GDBSD(0.2 mL/10 g),or with intraperitoneal injection of U0126(20 mg/kg)in addition to GDBSD gavage,with 15 male DL mice as control.After 4 weeks of treatment,copper content in testicular tissue of the mice was detected,and histopathology of the testes and epididymis was examined using HE staining and electron microscopy.TUNEL staining was used to identify apoptotic cells in the testes.The protein expressions of Bcl-2,Cytc,caspase-3,ERK,and p-ERK in the testicular tissue were evaluated with Western blotting,and BrdU-positive cells were detected with immunohistochemical labeling.Sperm density,viability,malformation rate and fertility levels of male mice were studied.Results Treatment with penicillamine and GDBSD obviously improved pathological changes of the testis,increased sperm density and motility,lowered sperm abnormality rate,fertility levels and increased testicular JOHNSEN score of TK mice,but the therapeutic effect of GDBSD was blocked by U0126.GDBSD treatment significantly lowered Cytc and caspase-3 expressions and increased Bcl-2 expression in the testicular tissue of TX mice(P<0.05),while U0126 treatment significantly lowered testicular Bcl-2 expression level.No significant differences were found in total protein expression levels of ERK1/2 among the 5 groups,but p-ERK protein expression was significantly reduced in WD and U0126 groups and increased in penicillamine and GDBSD groups.Conclusion GDBSD can improve spermatogenesis and enhance fertility of male TX mice with WD possibly by activating the ERK signaling pathway to enhance proliferation and reduce apoptosis of the spermatogenic cells.

Objective To investigate the therapeutic mechanism of Gandou Bushen Decoction(GDBSD)for improving reproductive disorders in male mouse models of Wilson disease(WD).Methods Sixty male homozygous TX mice were randomized equally into 4 groups and treated with daily gavage of saline(WD model group),penicillamine(0.09 g/kg),or GDBSD(0.2 mL/10 g),or with intraperitoneal injection of U0126(20 mg/kg)in addition to GDBSD gavage,with 15 male DL mice as control.After 4 weeks of treatment,copper content in testicular tissue of the mice was detected,and histopathology of the testes and epididymis was examined using HE staining and electron microscopy.TUNEL staining was used to identify apoptotic cells in the testes.The protein expressions of Bcl-2,Cytc,caspase-3,ERK,and p-ERK in the testicular tissue were evaluated with Western blotting,and BrdU-positive cells were detected with immunohistochemical labeling.Sperm density,viability,malformation rate and fertility levels of male mice were studied.Results Treatment with penicillamine and GDBSD obviously improved pathological changes of the testis,increased sperm density and motility,lowered sperm abnormality rate,fertility levels and increased testicular JOHNSEN score of TK mice,but the therapeutic effect of GDBSD was blocked by U0126.GDBSD treatment significantly lowered Cytc and caspase-3 expressions and increased Bcl-2 expression in the testicular tissue of TX mice(P<0.05),while U0126 treatment significantly lowered testicular Bcl-2 expression level.No significant differences were found in total protein expression levels of ERK1/2 among the 5 groups,but p-ERK protein expression was significantly reduced in WD and U0126 groups and increased in penicillamine and GDBSD groups.Conclusion GDBSD can improve spermatogenesis and enhance fertility of male TX mice with WD possibly by activating the ERK signaling pathway to enhance proliferation and reduce apoptosis of the spermatogenic cells.