Objective To compare the clinical effect of the conventional detection and detection of hepatitis B virus large protein , aiming at improving the detection rate of hepatitis B .Methods According to the principle of random selection ,80 cases of hepatitis B patients ,from September 2016 to February 2017 in our hospital ,were selected and were randomly divided into two groups ,40 ca-ses in the control group ,40 cases in the observation group .The observation group was adopted the detection of hepatitis B virus large protein while the control group were treated by conventional detection method .The detection rate of two groups was com-pared .Results The positive rate of hepatitis B virus infection by Real-timePCR for detection in observation group was 86 .25%(69/80) ,which was higher than 72 .50% (58/80) in control group by ELISA ,and the difference was statistically significant (P<0 .05) .The positive rate of HBV-DNA in control group was 77 .50% (31/40) which was lower than 90 .00% (36/40) in observa-tion group ,and the difference was statistically significant (P<0 .05) .Conclusion The conventional detection method and detection of hepatitis B virus large protein both can detect the hepatitis B virus ,and detection rate of detection of hepatitis B virus large pro-tein was higher .Detection of hepatitis B virus large protein is recommended for the detection of hepatitis B virus in order to reduce the misdiagnosis rate .