Objective To investigate the drug resistance and prevalence of integrons in clinical isolates of Pseudomonas aerugino‐sa(PA) and to analyze the correlation between presence of integrons and drug resistance .Methods A total of 260 non‐repetitive clinical isolates of PA were obtained from this Hospital;antimicrobial susceptibility testing was performed by using the K‐B disk diffusion methods;statistical analysis was performed by using the WHONET5 .0 software .Integrons were detected by using poly‐merase chain reaction(PCR) .Results Among the 260 clinical isolates of PA ,the resistance rates against 14 antibacterial agents from low to high were imipenem(18 .5% ) ,amikacin(18 .8% ) ,cefepime(20 .3% ) ,piperacillin/tazobactam(21 .5% ) ,ceftazidime pen‐tahydrate(22 .6% ) ,aztreonam(31 .9% ) ,tobramycin(31 .9% ) ,cefoperazone(38 .1% ) ,gentamicin(38 .8% ) ,piperacillin(41 .9% ) , ciprofloxacin(49 .2% ) ,cefotaxime(61 .9% ) and sulfamethoxazole/trimethoprim(70 .4% ) ,respectively .A total of 109 isolates pres‐ented Class Ⅰ integrons and 4 isolates presented class Ⅱintegrons ,while no class Ⅲ integrons were found in any isolates .Except for polymyxin ,the resistance rates of class Ⅰ integrons positive isolates against antibacterial agents examined in this study were significantly higher than those of class Ⅰ integrons negative isolates (P<0 .05) .Conclusion The classⅠand class Ⅱ integrons are the prevalent types in the local region ,which might be closely related to the drug resistance in clinical isolates of PA .