Objective? To explore the effects of case combined with Micro-lecture teaching in perioperative nursing teaching among gastrointestinal cancer patients. Methods? From January 2015 to December 2018, we selected 128 nursing students with practice in the Hangzhou First People's Hospital as subjects by convenience sampling. Nursing students with practice from January 2015 to December 2016 were in control group (n=64). A total of 64 practice nursing students from January 2017 to December 2018 were in observation group. Control group adopted case teaching, while observation group carried out case combined with Micro-lecture teaching. The teaching effects were assessed with the Self-Rating Scale for Self-Directedness in Learning (SRSSDL) and the Chinese version of the Competency Inventory for Nursing Students (CINS). Results? After teaching,the total scores of SRSSDL and CINS as well as dimension scores of observation group were higher than those in control group with statistical differences (P＜ 0.05). Conclusions? Case combined with Micro-lecture teaching can improve self-directedness learning ability and core competency of practice nursing students to guarantee the nursing teaching quality.