Including gut microbiota and oral microbiota, various microorganisms in different human ecosystem constitute the human microbiota, which play an important role in human metabolism, immunity and maintaining microecological homeostasis. Abnormal changes in gut microbiota known as dysbiosis may lead to metabolic abnormalities and inflammatory changes, which are closely related to disease states including hypertension, diabetes, inflammatory bowel disease, and autoimmune diseases. The main cause of coronary artery disease is coronary atherosclerosis, a chronic and progressive inflammatory disease. Many evidences have shown that there is a correlation between gut microbiota and coronary artery disease. Therefore, we aim to review the relationship between gut microbiota and coronary artery disease, and discuss the possible research directions and application prospects.

Objective:To explore the feasibility of applying quantitative flow ratio(QFR) to assess the degree of coronary artery functional stenosis before surgery, and to guide coronary artery bypass grafting(CABG) revascularization strategy.Methods:The study prospectively included a total of 154 patients who were electively treated with CABG in the 11th ward of the Department of Cardiac Surgery of Beijing Anzhen Hospital from January 2019 to September 2020, and their coronary angiography visually showed stenosis of the coronary artery to perform QFR analysis to know the diseased blood vessels. For functional stenosis, the surgeon was blinded to the results of QFR analysis before surgery. Collect its baseline data, perioperative data and recent clinical outcomes for summary analysis.Results:One year later, the coronary artery CTA showed that the occlusion rate of functionally significant disease(QFR<0.8) was 5.5%, and that of non-functionally significant disease(QFR≥0.8) was 15.6%. There was no difference in angina class or repeat interventions between patients with or without occluded bypass grafts.Conclusion:According to QFR analysis, coronary arteries with functional non-significant disease have a higher risk of grafts failure than those with functionally significant disease. For coronary arteries with negative QFR lesions, the risk of occlusion of arterial grafts is higher than that of venous. However, this finding is not significantly related to clinical prognosis, because patients with patency or occlusion of the grafts in non-significant lesions have not found excessive angina pectoris or repeated coronary interventions. QFR-guided selection of coronary surgery strategies is safe and feasible.

Objective     To investigate the early and long-outcomes of coronary artery bypass grafting(CABG) in acute myocardial infarction (AMI) patients with coronary artery disease(CAD)(age≤45 years). Methods     Data of 596 adult CAD patients (include AMI and Angina) who underwent CABG in our hospital were collected retrospectively from May 2010 to October 2018. In an AMI group, 234 were male patients with an average age of 41.59±3.79 years; 26 were female patients with an average age of 41.64±3.03 years. In an angina group, 280 were male patients with an average age of 42.19±2.90 years; 56 were female patients with an average age of 41.54±3.52 years. Preoperative baseline variables, perioperative mortality, major adverse cardiac and cerebrovascular events (MACCE) were compared between two group.  Results    There was no significant difference in all preoperative variables. Seven patients were died and the hospital mortality rate was 1.23% (1.54% vs. 0.89%, P=0.477). The complications including reoperation for bleeding, cerebral infarction, renal failure and atrial fibrillation arrhythmia were without significant difference between two group  (P>0.05). The intensive care unit stay duration (30.66±27.46 h vs. 23.96±15.11 h), intubation duration (22.54±22.31 h vs. 18.64±11.81 h) and hospitalization costs (97 186±33 741¥ vs. 90 081±24 537¥, P=0.003) were greater in the AMI group. The hospital mortality rate and complications rate were without significant difference between STEMI (ST segment elevated myocardial infarction) and NSTEMI (non-ST-segment elevated myocardial infarction) subgroups (P>0.05). The follow-up rate was 92.6% (546 patients) and the follow-up time was 4 (0.5 to 8.5) years. All cause-mortality rate was 3.85%(21 patients), and freedom MACCE was 72.2%. The freedom from MACCE, recurred angina and cerebral infarction were without significant difference, but AMI was associated with higher rate of PCI procedure.  Conclusion     CABG procedure in CAD patients under 45 years accompanied AMI is safety and reliable both in early and the long-term outcomes.

Charcot foot is a rare disease in clinic, its pathogenesis includes neurotrauma theory, neurovascular theory, comprehensive theory, and inflammatory factor theory. The disease is characterized by progressive joint and bone destruction of foot and ankle joint. Conventional X-ray examination is not sensitive to the early diagnosis of disease, the manifestation of CT and MRI of disease is characteristic and could be used to make a comprehensive evaluation of bone and soft tissue lesions of disease. It is not difficult to make a diagnosis based on characteristic findings of CT and MRI and clinical manifestations such as swelling, pain and skin temperature rising of foot and ankle. Charcot foot has multiple classification methods including anatomy, imaging and clinical classification. Improved Eichenholtz staging classification is most commonly used currently which could make a more comprehensive assessment of disease and guide treatment better. According to the stage of disease, treatment could be carried out including non-weight bearing and brace protection, drugs therapy and surgical treatment, etc. Early diagnosis, brace protection, could protect joint and delaying progression of deformity. There is no clear long-term and generally accepted conclusion about the efficacy of drug therapy. For advanced patients, surgical treatment must be actively performed to preserve a stable and functional ankle joint and reduce amputation rate.
Amputation ; Ankle Joint ; Arthropathy, Neurogenic ; Diabetic Foot ; Humans ; Radiography

OBJECTIVETo investigate the relationship between plasma miR-93-5p and the risk of esophageal cancer, as well as the influence of miR-93-5p on the biological function of esophageal cancer cells, exerted through exosomes.

METHODSThe expression of plasma miR-93-5p in esophageal cancer patients and healthy controls was analysed by real-time quantitative PCR. The influence of miR-93-5p on the risk and prognosis of esophageal carcinoma was analyzed by conditional logistic regression and survival analysis. The effect of miR-93-5p on the biological function of recipient cells was investigated by establishing an in vitro donor cell co-culture model. The target gene of miR-93-5p was validated by luciferase reporter assay and Western Blotting.

RESULTSUpregulation of plasma miR-93-5p expression significantly increases the risk of esophageal cancer and is associated with poor prognosis. miR-93-5p transferred by exosomes promotes the proliferation of recipient esophageal cancer cells and affects the expression of PTEN and its downstream proteins p21 and cyclin D1.

CONCLUSIONOur study provides a reference for the identification of biomarkers for the diagnosis and prognosis of esophageal cancer.

Aged ; Cell Communication ; China ; Esophageal Neoplasms ; physiopathology ; Exosomes ; physiology ; Female ; Humans ; Male ; MicroRNAs ; metabolism ; Middle Aged ; PTEN Phosphohydrolase ; genetics ; metabolism ; Risk

Objective To investigate the effect of Kangfu Xiaoyan suppositories on nuclear factor κB (NF-κB),tumor necrosis factor-α (TNF-α) and intercellular adhesion molecule-1 (ICAM-1) levels of rats with sequela of pelvic inflammatory diseases (SPID) Methods Ninety specific pathogen free (SPF) Wistar rats were randomly divided into normal group,sham group,model group,experimental group and positive control group,18 rats in each group.The normal group was given no intervention and normal feeding.Sham group only switch abdominal surgery.The rats of model group,experimental group and positive control group were given foreign body implantation to induce SPID.After 2 weeks,the experimental group were given Kangfu Xiaoyan suppositories 5.04 g · kg-1 d-1,retention enema;and the positive control group were given clindamycin capsules 0.04 g · kg-1 · d-1,intragastric administration for 21 d.The normal group,sham group and model group were not disposed.The uterine swelling rate and swelling inhibition rate were observed in each group.The immunohistochemical method was used to detect the levels of NF-κB,TNF-α and ICAM-1 of rats in each group.Results The uterine swelling rates in sham group,model group,experimental group and positive control group were (10.09 ±5.05)％,(33.98 ± 15.61)％,(7.48 ±8.21)％,(12.31 ±6.67)％.The uterine swelling rate in model group was higher than that in experimental group,with significant difference (P＜0.05).The levels of NF-κB,TNF-α and ICAM-1 in experimental group were(1.33 ±0.82),(1.83 ±0.75),(0.17 ±0.41) ng · L-1,had significant difference with those in model group,which were (4.00 ± 1.79),(5.17 ±2.40),(2.00 ± 1.10) ng · L-1 (P ＜0.05).Conclusion The therapeutic effect of Kangfu Xiaoyan suppositories on SPID rats is related to the inhibition of swelling of uterus and the decrease the levels of inflammatory factors.

OBJECTIVETo investigate the inductive therapeutic effects of imatinib combined with VP low dose regiment on adult patients with Ph-positive acute lymphoblastic leukemia (Ph(+) ALL).

METHODSFourteen newly diagnosed adult patients with Ph(+) ALL were treated with VP regimen, and imatinib (400 mg/d) was added at the 8(th) day. VP regimen would be stopped when neutropenia lasted for 1 week or complicated with infection, fever, etc. Therapeutic effects were assessed by bone marrow morphology and quantitative analysis of BCR/ABL:ABL at the 28(th) - 33(rd) day. Patients could be treated with imatinib combined with chemotherapy for consolidation and maintenance therapy or were treated with allogeneic hematopoietic stem cell transplantation after complete remission.

RESULTSFourteen cases obtained CR1 after first course of treatment, the median decline of BCR/ABA:ABL was 55.89 (10.25 -180.97) %; during the induction chemotherapy, pulmonary infection occurred in 3 patients, diarrhea in 1 patients, facial edema in 3 patients, however, all these patients were cured after symptomatic treatment, only 1 patient died of relapse after transplantation.

CONCLUSIONIn the period of tyrosine kinase inhibitor (TKI), inductive chemotherapy combined with imatinib and low dose VP can obtaine satisfactory CR rate and decrease the toxicity of the traditional drugs. It is suggested that TKI combined with VP regimen chemotherapy can achieve CR1 and make possible for allo-HSCT, from which patients can achieve the long-term survival.

Adult ; Antineoplastic Combined Chemotherapy Protocols ; Bone Marrow ; Cisplatin ; Fusion Proteins, bcr-abl ; Hematopoietic Stem Cell Transplantation ; Humans ; Imatinib Mesylate ; Induction Chemotherapy ; Neutropenia ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; Protein Kinase Inhibitors ; Recurrence ; Remission Induction ; Transplantation, Homologous ; Vindesine

Objective To compare the pelvis free liquid quantity deepness measured by ultrasound and actual blood volume in abdominal cavity observed during operation in ectopic pregnancy patients, so as to predict the pre-operation intra-abdominal hemorrhage volume. Methods Five hundred patients with ectopic pregnancy receiving emergent operation were included in the present study. The patients took lithotomy position and were examined by vaginal probe before operation. The pelvis free liquid quantity measured by ultrasound (x) and the actual blood volume in abdominal cavity (y) observed during operation were subjected to statistical analysis using SPSS 16. 0 software. Results The pelvis free liquid measured by ultrasound and the actual quantity of blood in abdominal cavity accorded with the following equation:;y = 43. 306 e0.704x R2 = 0. 983, P<0. 05. The actual volume of blood in pelvis could be calculated by the equation. When the quantities of free liquid in pelvis were 3 cm, 3.5 cm, 4.0 cm, and 4.5 cm, the actual volumes of blood in pelvis were 357.91 ml, 508.92 ml, 732.64 ml, and 1028.95 ml, respectively. Conclusion The quantity of intra-abdominal hemorrhage can be predicted by the pelvis free liquid deepness in ectopic pregnancy patients, which may pave a way for more effective treatment of the patients.

Chemokine CXCL12 ; Child ; Humans ; Neoplasm Metastasis ; Neoplasms ; pathology ; Receptors, CXCR4